Mouse Sonic Hedgehog/Shh C-Terminus Antibody | R&D Systems AF445 product information

product summary

company name :

R&D Systems

product type :

antibody

product name :

Mouse Sonic Hedgehog/Shh C-Terminus Antibody

catalog :

AF445

quantity :

100 ug (also 25 ug)

price :

469 USD

clonality :

polyclonal

host :

domestic goat

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

R&D Systems product webpage

citations: 5

Reference
Mafi A, Purohit R, Vielmas E, Lauinger A, Lam B, Cheng Y, et al. Hedgehog proteins create a dynamic cholesterol interface. PLoS ONE. 2021;16:e0246814 pubmed publisher
Purohit R, Peng D, Vielmas E, Ondrus A. Dual roles of the sterol recognition region in Hedgehog protein modification. Commun Biol. 2020;3:250 pubmed publisher
Matos I, Asare A, Levorse J, Ouspenskaia T, de la Cruz Racelis J, Schuhmacher L, et al. Progenitors oppositely polarize WNT activators and inhibitors to orchestrate tissue development. elife. 2020;9: pubmed publisher
Rodr xed guez Seguel E, Villamayor L, Arroyo N, de Andr xe9 s M, Real F, Mart xed n F, et al. Loss of GATA4 causes ectopic pancreas in the stomach. J Pathol. 2020;250:362-373 pubmed publisher
Andreu Cervera A, Anselme I, Karam A, Laclef C, Catala M, Schneider Maunoury S. The Ciliopathy Gene Ftm/Rpgrip1l Controls Mouse Forebrain Patterning via Region-Specific Modulation of Hedgehog/Gli Signaling. J Neurosci. 2019;39:2398-2415 pubmed publisher

product information

master code :

AF445

SKU :

AF445

product name :

Mouse Sonic Hedgehog/Shh C-Terminus Antibody

unit size :

100 ug (also 25 ug)

description :

The Mouse Sonic Hedgehog/Shh C-Terminus Antibody from R&D Systems is a goat polyclonal antibody to Sonic Hedgehog/Shh. This antibody reacts with human,mouse. The Mouse Sonic Hedgehog/Shh C-Terminus Antibody has been validated for the following applications: Immunohistochemistry,Flow Cytometry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunocytochemistry.

target :

Sonic Hedgehog/Shh

category :

Primary Antibodies

buffer :

Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.

clonality :

Polyclonal

concentration :

LYOPH

conjugate :

Unconjugated

host :

Goat

immunogen :

E. coli -derived recombinant mouse Sonic Hedgehog, Cys25-Ser437 with a Lys122Arg substitution, Accession # Q62226

isotype :

IgG

purity :

Antigen Affinity-purified

species :

Human,Mouse

specificity :

Detects mouse Shh C-terminal peptide in direct ELISAs and Western blots. In these formats, less than 5% cross-reactivity with recombinant mouse (rm) Dhh C-terminal peptide is observed and less than 1% cross-reactivity with a 6X histidine-tagged rmShh N-terminal peptide (amino acids 25-198) is observed. Detects human and mouse Sonic Hedgehog protein in Western blots. Shh C-terminal peptide specific IgG was purified by first passing the goat sera over a mouse Shh N-terminal peptide affinity column. The unbound fraction from the mouse Shh N-terminal peptide affinity column was subsequently purified using a mouse Shh C-terminal peptide affinity column.

gene symbol :

SHH

top caption :

Detection of Human and Mouse Sonic Hedgehog/Shh antibody by Western Blot.

accessionNumbers :

Q62226

applications :

Flow Cytometry,Western Blot,Immunohistochemistry,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunocytochemistry

USD :

469 USD

alt names :

HHG1, HHG-1, HLP3, HPE3, MCOPCB5, MCOPCB5sonic hedgehog (Drosophila) homolog, Shh, ShhNC, SMMCI, SMMCIsonic hedgehog homolog (Drosophila), sonic hedgehog, sonic hedgehog homolog, sonic hedgehog protein, TPT, TPTPS

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage