product summary
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company name :
R&D Systems
product type :
antibody
product name :
Human Nephrin Antibody
catalog :
AF4269
quantity :
100 ug (also 25 ug)
price :
519 USD
clonality :
polyclonal
host :
domestic sheep
conjugate :
nonconjugated
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
citations: 47
Reference
Shi M, Crouse B, Sundaram N, Pode Shakked N, Ester L, Zhang W, et al. Integrating collecting systems in kidney organoids through fusion of distal nephron to ureteric bud. bioRxiv. 2024;: pubmed publisher
Xi G, Lamba S, Mysh M, Poulton J. Oxidative Stress Contributes to Slit Diaphragm Defects Caused by Disruption of Endocytosis. Kidney Int Rep. 2024;9:451-463 pubmed publisher
Butt L, Unnersj xf6 Jess D, Reilly D, Hahnfeldt R, Rinschen M, Bozek K, et al. In vivo characterization of a podocyte-expressed short podocin isoform. BMC Nephrol. 2023;24:378 pubmed publisher
Melica M, Angelotti M, Antonelli G, Peired A, Conte C, De Chiara L, et al. Preparation of Human Kidney Progenitor Cultures and Their Differentiation into Podocytes. Bio Protoc. 2023;13:e4757 pubmed publisher
Lass xe9 M, El Saghir J, Berthier C, Eddy S, Fischer M, Laufer S, et al. An integrated organoid omics map extends modeling potential of kidney disease. Nat Commun. 2023;14:4903 pubmed publisher
Charrin E, Dabaghie D, Sen I, Unnersj xf6 Jess D, M xf6 ller Hackbarth K, Burmakin M, et al. Soluble Klotho protects against glomerular injury through regulation of ER stress response. Commun Biol. 2023;6:208 pubmed publisher
Park K, Lee J, Lee S, Jeong I, Park S, Kim J, et al. Deep learning predicts the differentiation of kidney organoids derived from human induced pluripotent stem cells. Kidney Res Clin Pract. 2023;42:75-85 pubmed publisher
Gaykema L, van Nieuwland R, Dekkers M, van Essen M, Heidt S, Zaldumbide A, et al. Inhibition of complement activation by CD55 overexpression in human induced pluripotent stem cell derived kidney organoids. Front Immunol. 2022;13:1058763 pubmed publisher
Vandorpe D, Heneghan J, Waitzman J, McCarthy G, Blasio A, Magraner J, et al. Apolipoprotein L1 (APOL1) cation current in HEK-293 cells and in human podocytes. Pflugers Arch. 2022;: pubmed publisher
Wang G, Heijs B, Kostidis S, Rietjens R, Koning M, Yuan L, et al. Spatial dynamic metabolomics identifies metabolic cell fate trajectories in human kidney differentiation. Cell Stem Cell. 2022;29:1580-1593.e7 pubmed publisher
Vanslambrouck J, Wilson S, Tan K, Groenewegen E, Rudraraju R, Neil J, et al. Enhanced metanephric specification to functional proximal tubule enables toxicity screening and infectious disease modelling in kidney organoids. Nat Commun. 2022;13:5943 pubmed publisher
Wang G, Heijs B, Kostidis S, Mahfouz A, Rietjens R, Bijkerk R, et al. Analyzing cell-type-specific dynamics of metabolism in kidney repair. Nat Metab. 2022;4:1109-1118 pubmed publisher
Chung H, Bui Marinos M, Rahmani W, Corcoran J, Chun J. Infecting kidney organoids with a cDNA reporter clone of SARS-CoV-2. STAR Protoc. 2022;3:101617 pubmed publisher
Bejoy J, Farry J, Peek J, Cabatu M, Williams F, Welch R, et al. Podocytes derived from human induced pluripotent stem cells: characterization, comparison, and modeling of diabetic kidney disease. Stem Cell Res Ther. 2022;13:355 pubmed publisher
Westerling Bui A, Fast E, Soare T, Venkatachalan S, DeRan M, Fanelli A, et al. Transplanted organoids empower human preclinical assessment of drug candidate for the clinic. Sci Adv. 2022;8:eabj5633 pubmed publisher
Won N, Castillo Prado J, Tan X, Ford J, Heath D, Mazilescu L, et al. Ex Vivo Perfusion Using a Mathematical Modeled, Controlled Gas Exchange Self-Contained Bioreactor Can Maintain a Mouse Kidney for Seven Days. Cells. 2022;11: pubmed publisher
Vanslambrouck J, Wilson S, Tan K, Groenewegen E, Rudraraju R, Neil J, et al. Enhanced metanephric specification to functional proximal tubule enables toxicity screening and infectious disease modelling in kidney organoids. bioRxiv. 2022;: pubmed publisher
Garreta E, Prado P, Stanifer M, Monteil V, Marco A, Ullate Agote A, et al. A diabetic milieu increases ACE2 expression and cellular susceptibility to SARS-CoV-2 infections in human kidney organoids and patient cells. Cell Metab. 2022;34:857-873.e9 pubmed publisher
Jansen J, van den Berge B, van den Broek M, Maas R, Daviran D, Willemsen B, et al. Human pluripotent stem cell-derived kidney organoids for personalized congenital and idiopathic nephrotic syndrome modeling. Development. 2022;149: pubmed publisher
Lawrence M, Elhendawi M, Morlock M, Liu W, Liu S, Palakkan A, et al. Human iPSC-derived renal organoids engineered to report oxidative stress can predict drug-induced toxicity. iScience. 2022;25:103884 pubmed publisher
Rahmani W, Chung H, Sinha S, Bui Marinos M, Arora R, Jaffer A, et al. Attenuation of SARS-CoV-2 infection by losartan in human kidney organoids. iScience. 2022;25:103818 pubmed publisher
Jansen J, Reimer K, Nagai J, Varghese F, Overheul G, de Beer M, et al. SARS-CoV-2 infects the human kidney and drives fibrosis in kidney organoids. Cell Stem Cell. 2022;29:217-231.e8 pubmed publisher
Miesen L, Bandi P, Willemsen B, Mooren F, Strieder T, Boldrini E, et al. Parietal epithelial cells maintain the epithelial cell continuum forming Bowman's space in focal segmental glomerulosclerosis. Dis Model Mech. 2022;15: pubmed publisher
Ungricht R, Guibbal L, Lasbennes M, Orsini V, Beibel M, Waldt A, et al. Genome-wide screening in human kidney organoids identifies developmental and disease-related aspects of nephrogenesis. Cell Stem Cell. 2022;29:160-175.e7 pubmed publisher
Lee K, Lee J, Lee P, Jeon B, Song M, Kwak S, et al. Inhibition of O-GlcNAcylation protects from Shiga toxin-mediated cell injury and lethality in host. EMBO Mol Med. 2022;14:e14678 pubmed publisher
Bejoy J, Qian E, WOODARD L. Accelerated protocol for the differentiation of podocytes from human pluripotent stem cells. STAR Protoc. 2021;2:100898 pubmed publisher
Ueda S, Tominaga T, Ochi A, Sakurai A, Nishimura K, Shibata E, et al. TGF-β1 is involved in senescence-related pathways in glomerular endothelial cells via p16 translocation and p21 induction. Sci Rep. 2021;11:21643 pubmed publisher
Kim J, Kim H, Nam S, Lee J, Cho H, Kim T, et al. Human kidney organoids reveal the role of glutathione in Fabry disease. Exp Mol Med. 2021;53:1580-1591 pubmed publisher
Mangold N, Pippin J, Unnersjoe Jess D, Koehler S, Shankland S, Brähler S, et al. The Atypical Cyclin-Dependent Kinase 5 (Cdk5) Guards Podocytes from Apoptosis in Glomerular Disease While Being Dispensable for Podocyte Development. Cells. 2021;10: pubmed publisher
Sung S. Co-immunostaining of ICAM-1, ICAM-2, and CD31 in Mouse Kidney Glomeruli. Bio Protoc. 2020;10:e3663 pubmed publisher
Wang Q, Xiong Y, Zhang S, Sui Y, Yu C, Liu P, et al. The Dynamics of Metabolic Characterization in iPSC-Derived Kidney Organoid Differentiation via a Comparative Omics Approach. Front Genet. 2021;12:632810 pubmed publisher
Sander V, Przepiorski A, Crunk A, Hukriede N, Holm T, Davidson A. Protocol for Large-Scale Production of Kidney Organoids from Human Pluripotent Stem Cells. STAR Protoc. 2020;1:100150 pubmed publisher
Uchimura K, Wu H, Yoshimura Y, Humphreys B. Human Pluripotent Stem Cell-Derived Kidney Organoids with Improved Collecting Duct Maturation and Injury Modeling. Cell Rep. 2020;33:108514 pubmed publisher
Kim J, Nam S, Seo E, Lee J, Kim D, Ju J, et al. Human kidney organoids model the tacrolimus nephrotoxicity and elucidate the role of autophagy. Korean J Intern Med. 2021;36:1420-1436 pubmed publisher
Usui J, Yaguchi M, Yamazaki S, Takahashi Kobayashi M, Kawamura T, Kaneko S, et al. Transcription factor 21 expression in injured podocytes of glomerular diseases. Sci Rep. 2020;10:11516 pubmed publisher
Monteil V, Kwon H, Prado P, Hagelkr ys A, Wimmer R, Stahl M, et al. Inhibition of SARS-CoV-2 Infections in Engineered Human Tissues Using Clinical-Grade Soluble Human ACE2. Cell. 2020;181:905-913.e7 pubmed publisher
Subramanian A, Sidhom E, Emani M, Vernon K, Sahakian N, Zhou Y, et al. Single cell census of human kidney organoids shows reproducibility and diminished off-target cells after transplantation. Nat Commun. 2019;10:5462 pubmed publisher
Vanslambrouck J, Wilson S, Tan K, Soo J, Scurr M, Spijker H, et al. A Toolbox to Characterize Human Induced Pluripotent Stem Cell-Derived Kidney Cell Types and Organoids. J Am Soc Nephrol. 2019;30:1811-1823 pubmed publisher
Howden S, Vanslambrouck J, Wilson S, Tan K, Little M. Reporter-based fate mapping in human kidney organoids confirms nephron lineage relationships and reveals synchronous nephron formation. EMBO Rep. 2019;20: pubmed publisher
Kumar S, Er P, Lawlor K, Motazedian A, Scurr M, Ghobrial I, et al. Kidney micro-organoids in suspension culture as a scalable source of human pluripotent stem cell-derived kidney cells. Development. 2019;146: pubmed publisher
Garreta E, Prado P, Tarantino C, Oria R, Fanlo L, Marti E, et al. Fine tuning the extracellular environment accelerates the derivation of kidney organoids from human pluripotent stem cells. Nat Mater. 2019;18:397-405 pubmed publisher
Harder J, Menon R, Otto E, Zhou J, Eddy S, Wys N, et al. Organoid single cell profiling identifies a transcriptional signature of glomerular disease. JCI Insight. 2019;4: pubmed publisher
Phipson B, Er P, Combes A, Forbes T, Howden S, Zappia L, et al. Evaluation of variability in human kidney organoids. Nat Methods. 2019;16:79-87 pubmed publisher
Hale L, Howden S, Phipson B, Lonsdale A, Er P, Ghobrial I, et al. 3D organoid-derived human glomeruli for personalised podocyte disease modelling and drug screening. Nat Commun. 2018;9:5167 pubmed publisher
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product information
master code :
AF4269
SKU :
AF4269
product name :
Human Nephrin Antibody
unit size :
100 ug (also 25 ug)
description :
The Human Nephrin Antibody from R&D Systems is a sheep polyclonal antibody to Nephrin. This antibody reacts with human,mouse,rat,transgenic mouse. The Human Nephrin Antibody has been validated for the following applications: Western Blot,Immunohistochemistry,Immunocytochemistry,Flow Cytometry,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunocytochemistry/ Immunofluorescence,Simple Western.
target :
Nephrin
category :
Primary Antibodies
buffer :
Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.
clonality :
Polyclonal
concentration :
LYOPH
conjugate :
Unconjugated
host :
Sheep
immunogen :
Mouse myeloma cell line NS0-derived recombinant human Nephrin, Gln23-Thr1029, Accession # O60500
isotype :
IgG
purity :
Antigen Affinity-purified
species :
Human,Mouse,Rat,Transgenic Mouse
specificity :
Detects human Nephrin in direct ELISAs and Western blots. In direct ELISAs, less than 20% cross-reactivity with recombinant mouse Nephrin is observed.
gene symbol :
NPHS1
top caption :
Detection of Human Nephrin antibody by Western Blot.
accessionNumbers :
O60500
applications :
Immunohistochemistry,Immunocytochemistry,Flow Cytometry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunocytochemistry/ Immunofluorescence,Simple Western
USD :
519 USD
alt names :
CNF, nephrin, nephrosis 1, congenital, Finnish type (nephrin), NPHNCNF, NPHS1, Renal glomerulus-specific cell adhesion receptor
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.
more info or order :
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.