Human Fibroblast Activation Protein alpha /FAP Antibody | R&D Systems AF3715 product information

product summary

company name :

R&D Systems

product type :

antibody

product name :

Human Fibroblast Activation Protein alpha /FAP Antibody

catalog :

AF3715

quantity :

100 ug (also 25 ug)

price :

509 USD

clonality :

polyclonal

host :

domestic sheep

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

more info or order :

R&D Systems product webpage

citations: 36

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:2000; fig s5	Ray U, Roy D, Jin L, Thirusangu P, Staub J, Xiao Y, et al. Group III phospholipase A2 downregulation attenuated survival and metastasis in ovarian cancer and promotes chemo-sensitization. J Exp Clin Cancer Res. 2021;40:182 pubmed publisher
western blot; human; 1:2000; fig s5	Dwir D, Cabungcal J, Xin L, Giangreco B, Parietti E, Cleusix M, et al. Timely N-Acetyl-Cysteine and Environmental Enrichment Rescue Oxidative Stress-Induced Parvalbumin Interneuron Impairments via MMP9/RAGE Pathway: A Translational Approach for Early Intervention in Psychosis. Schizophr Bull. 2021;47:1782-1794 pubmed publisher
western blot; human; loading ...; fig 1a	Ganig N, Baenke F, Thepkaysone M, Lin K, Rao V, Wong F, et al. Proteomic Analyses of Fibroblast- and Serum-Derived Exosomes Identify QSOX1 as a Marker for Non-invasive Detection of Colorectal Cancer. Cancers (Basel). 2021;13: pubmed publisher
	Wijesinghe S, Badoume A, Nanus D, Sharma Oates A, Farah H, Certo M, et al. Obesity defined molecular endotypes in the synovium of patients with osteoarthritis provides a rationale for therapeutic targeting of fibroblast subsets. Clin Transl Med. 2023;13:e1232 pubmed publisher
	Mori N, Jin J, Krishnamachary B, Mironchik Y, Wildes F, Vesuna F, et al. Functional roles of FAP-α in metabolism, migration and invasion of human cancer cells. Front Oncol. 2023;13:1068405 pubmed publisher
	Pappalardo A, Alvarez Cespedes D, Fang S, Herschman A, Jeon E, Myers K, et al. Engineering edgeless human skin with enhanced biomechanical properties. Sci Adv. 2023;9:eade2514 pubmed publisher
	Baltazar T, Jiang B, Moncayo A, Merola J, Albanna M, Saltzman W, et al. 3D bioprinting of an implantable xeno-free vascularized human skin graft. Bioeng Transl Med. 2023;8:e10324 pubmed publisher
	Fan A, Wu G, Wang J, Lu L, Wang J, Wei H, et al. Inhibition of fibroblast activation protein ameliorates cartilage matrix degradation and osteoarthritis progression. Bone Res. 2023;11:3 pubmed publisher
	Jin J, Barnett J, Krishnamachary B, Mironchik Y, Luo C, Kobayashi H, et al. Evaluating near-infrared photoimmunotherapy for targeting fibroblast activation protein-α expressing cells in vitro and in vivo. Cancer Sci. 2023;114:236-246 pubmed publisher
	Li B, Ding Z, Calbay O, Li Y, Li T, Jin L, et al. FAP is critical for ovarian cancer cell survival by sustaining NF-κB activation through recruitment of PRKDC in lipid rafts. Cancer Gene Ther. 2022;: pubmed publisher
	Zeng W, Xiong L, Wu W, Li S, Liu J, Yang L, et al. CCL18 signaling from tumor-associated macrophages activates fibroblasts to adopt a chemoresistance-inducing phenotype. Oncogene. 2022;: pubmed publisher
	Marangoni R, Datta P, Paine A, Duemmel S, Nuzzo M, Sherwood L, et al. Thy-1 plays a pathogenic role and is a potential biomarker for skin fibrosis in scleroderma. JCI Insight. 2022;7: pubmed publisher
	Lindgren M, Rask G, Jonsson J, Berglund A, Lundin C, Jonsson P, et al. Type IV Collagen in Human Colorectal Liver Metastases-Cellular Origin and a Circulating Biomarker. Cancers (Basel). 2022;14: pubmed publisher
	Ou L, Wang H, Huang H, Zhou Z, Lin Q, Guo Y, et al. Preclinical platforms to study therapeutic efficacy of human γδ T cells. Clin Transl Med. 2022;12:e814 pubmed publisher
	Verginadis I, Avgousti H, Monslow J, Skoufos G, Chinga F, Kim K, et al. A stromal Integrated Stress Response activates perivascular cancer-associated fibroblasts to drive angiogenesis and tumour progression. Nat Cell Biol. 2022;24:940-953 pubmed publisher
	Ye G, Huang M, Li Y, Ouyang J, Chen M, Wen Q, et al. The FAP α -activated prodrug Z-GP-DAVLBH inhibits the growth and pulmonary metastasis of osteosarcoma cells by suppressing the AXL pathway. Acta Pharm Sin B. 2022;12:1288-1304 pubmed publisher
	De Vargas Roditi L, Jacobs A, Rueschoff J, Bankhead P, Chevrier S, Jackson H, et al. Single-cell proteomics defines the cellular heterogeneity of localized prostate cancer. Cell Rep Med. 2022;3:100604 pubmed publisher
	Mathieson L, O Connor R, Stewart H, Shaw P, Dhaliwal K, Williams G, et al. Fibroblast Activation Protein Specific Optical Imaging in Non-Small Cell Lung Cancer. Front Oncol. 2022;12:834350 pubmed publisher
	Chivu Economescu M, Necula L, Matei L, Dragu D, Bleotu C, Sorop A, et al. Collagen Family and Other Matrix Remodeling Proteins Identified by Bioinformatics Analysis as Hub Genes Involved in Gastric Cancer Progression and Prognosis. Int J Mol Sci. 2022;23: pubmed publisher
	Opzoomer J, Anstee J, Dean I, Hill E, Bouybayoune I, Caron J, et al. Macrophages orchestrate the expansion of a proangiogenic perivascular niche during cancer progression. Sci Adv. 2021;7:eabg9518 pubmed publisher
	Friedrich M, Pohin M, Jackson M, Korsunsky I, Bullers S, Rue Albrecht K, et al. IL-1-driven stromal-neutrophil interactions define a subset of patients with inflammatory bowel disease that does not respond to therapies. Nat Med. 2021;27:1970-1981 pubmed publisher
	Shimizu M, Koma Y, Sakamoto H, Tsukamoto S, Kitamura Y, Urakami S, et al. Metallothionein 2A Expression in Cancer-Associated Fibroblasts and Cancer Cells Promotes Esophageal Squamous Cell Carcinoma Progression. Cancers (Basel). 2021;13: pubmed publisher
	O Connor R, Chauhan V, Mathieson L, Titmarsh H, Koppensteiner L, Young I, et al. T cells drive negative feedback mechanisms in cancer associated fibroblasts, promoting expression of co-inhibitory ligands, CD73 and IL-27 in non-small cell lung cancer. Oncoimmunology. 2021;10:1940675 pubmed publisher
	Bruijn L, van den Akker B, van Rhijn C, Hamming J, Lindeman J. Extreme Diversity of the Human Vascular Mesenchymal Cell Landscape. J Am Heart Assoc. 2020;9:e017094 pubmed publisher
	Cho C, Mukherjee R, Peck A, Sun Y, McBrearty N, Katlinski K, et al. Cancer-associated fibroblasts downregulate type I interferon receptor to stimulate intratumoral stromagenesis. Oncogene. 2020;39:6129-6137 pubmed publisher
	Shirasu N, Shibaguchi H, Yamada H, Kuroki M, Yasunaga S. Highly versatile cancer photoimmunotherapy using photosensitizer-conjugated avidin and biotin-conjugated targeting antibodies. Cancer Cell Int. 2019;19:299 pubmed publisher
	Correa Gallegos D, Jiang D, Christ S, Ramesh P, Ye H, Wannemacher J, et al. Patch repair of deep wounds by mobilized fascia. Nature. 2019;576:287-292 pubmed publisher
	Wagner J, Rapsomaniki M, Chevrier S, Anzeneder T, Langwieder C, Dykgers A, et al. A Single-Cell Atlas of the Tumor and Immune Ecosystem of Human Breast Cancer. Cell. 2019;177:1330-1345.e18 pubmed publisher
	Sandberg T, Stuart M, Oosting J, Tollenaar R, Sier C, Mesker W. Increased expression of cancer-associated fibroblast markers at the invasive front and its association with tumor-stroma ratio in colorectal cancer. BMC Cancer. 2019;19:284 pubmed publisher
	Higashino N, Koma Y, Hosono M, Takase N, Okamoto M, Kodaira H, et al. Fibroblast activation protein-positive fibroblasts promote tumor progression through secretion of CCL2 and interleukin-6 in esophageal squamous cell carcinoma. Lab Invest. 2019;99:777-792 pubmed publisher
	Lakins M, Ghorani E, Munir H, Martins C, Shields J. Cancer-associated fibroblasts induce antigen-specific deletion of CD8 + T Cells to protect tumour cells. Nat Commun. 2018;9:948 pubmed publisher
	Chai X, Sun G, Fang Y, Hu L, Liu X, Zhang X. Tumor-targeting efficacy of a BF211 prodrug through hydrolysis by fibroblast activation protein-?. Acta Pharmacol Sin. 2017;: pubmed publisher
	Lo A, Li C, Buza E, Blomberg R, Govindaraju P, Avery D, et al. Fibroblast activation protein augments progression and metastasis of pancreatic ductal adenocarcinoma. JCI Insight. 2017;2: pubmed publisher
	Nagaraja A, Dood R, Armaiz Pena G, Kang Y, Wu S, Allen J, et al. Adrenergic-mediated increases in INHBA drive CAF phenotype and collagens. JCI Insight. 2017;2: pubmed publisher
	Feng X, Wang Q, Liao Y, Zhou X, Wang Y, Liu W, et al. A synthetic urinary probe-coated nanoparticles sensitive to fibroblast activation protein α for solid tumor diagnosis. Int J Nanomedicine. 2017;12:5359-5372 pubmed publisher
	Fan M, Zhu Q, Li H, Ra H, Majumdar S, Gulick D, et al. Fibroblast Activation Protein (FAP) Accelerates Collagen Degradation and Clearance from Lungs in Mice. J Biol Chem. 2016;291:8070-89 pubmed publisher

product information

brand :

R&D Systems

master code :

AF3715

SKU :

AF3715

product name :

Human Fibroblast Activation Protein alpha /FAP Antibody

unit size :

100 ug (also 25 ug)

seo description :

The Human Fibroblast Activation Protein alpha /FAP Antibody from R&D Systems is a sheep polyclonal antibody to Fibroblast Activation Protein alpha/FAP. This antibody reacts with human,mouse,transgenic mouse. The Human Fibroblast Activation Protein alpha /FAP Antibody has been validated for the following applications: Flow Cytometry,Western Blot,Immunocytochemistry,Immunohistochemistry,CAR-T (Flow Cytometry),Photoimmunotherapy,CAR-T (Flow Cytomety),Immunoprecipitation,Immunohistochemistry-Paraffin,Simple Western,Knockout Validated,Dual RNAscope ISH-IHC Compatible.

target :

Fibroblast Activation Protein alpha/FAP

category :

Primary Antibodies

buffer :

Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.

clonality :

Polyclonal

concentration :

LYOPH

conjugate :

Unconjugated

dilution :

Western Blot 0.5 ug/mL, Simple Western 10 ug/mL, Immunohistochemistry 5-15 ug/mL, Immunoprecipitation 25 ug/mL, Knockout Validated 0.5 ug/mL, Dual RNAscope ISH-IHC 5-15 ug/mL

host :

Sheep

immunogen :

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Fibroblast Activation Protein alpha /FAP, Leu26-Asp760, Accession # Q12884

isotype :

IgG

purity :

Antigen Affinity-purified

species :

Human,Mouse,Transgenic Mouse

specificity :

Detects human Fibroblast Activation Protein alpha /FAP in direct ELISAs and Western blots.

gene symbol :

FAP

top caption :

Detection of Human Fibroblast Activation Protein a/FAP antibody by Western Blot.

accessionNumbers :

Q12884

applications :

Simple Western,Knockout Validated,Dual RNAscope ISH-IHC Compatible,CAR-T (Flow Cytometry),Photoimmunotherapy,CAR-T (Flow Cytomety),Flow Cytometry,Immunohistochemistry,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Immunocytochemistry

USD :

509 USD

alt names :

170 kDa melanoma membrane-bound gelatinase, DKFZp686G13158, DPPIV, EC 3.4.21.-, FAP, FAPA, Fibroblast activation protein alpha, fibroblast activation protein, alpha, Integral membrane serine protease, seprase, vibronectin

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage