Human KLF4 Antibody | R&D Systems AF3640 product information

product summary

company name :

R&D Systems

product type :

antibody

product name :

Human KLF4 Antibody

catalog :

AF3640

quantity :

100 ug (also 25 ug)

price :

499 USD

clonality :

polyclonal

host :

domestic goat

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation, immunohistochemistry - paraffin section, western blot knockout validation

more info or order :

R&D Systems product webpage

citations: 30

Published Application/Species/Sample/Dilution	Reference
western blot knockout validation; human; 1:1000; loading ...; fig 6c	Wang P, Zhao L, Gong S, Xiong S, Wang J, Zou D, et al. HIF1α/HIF2α-Sox2/Klf4 promotes the malignant progression of glioblastoma via the EGFR-PI3K/AKT signalling pathway with positive feedback under hypoxia. Cell Death Dis. 2021;12:312 pubmed publisher
immunohistochemistry - paraffin section; human; 4 ug/ml; loading ...; fig 1c, 5h western blot; human; 1 ug/ml; loading ...; fig 5c	Sünderhauf A, Hicken M, Schlichting H, Skibbe K, Ragab M, Raschdorf A, et al. Loss of Mucosal p32/gC1qR/HABP1 Triggers Energy Deficiency and Impairs Goblet Cell Differentiation in Ulcerative Colitis. Cell Mol Gastroenterol Hepatol. 2021;12:229-250 pubmed publisher
	Paredes F, Williams H, Liu X, Holden C, Bogan B, Wang Y, et al. The mitochondrial protease ClpP is a druggable target that controls VSMC phenotype by a SIRT1-dependent mechanism. Redox Biol. 2024;73:103203 pubmed publisher
	Willet S, Thanintorn N, McNeill H, Huh S, Ornitz D, Huh W, et al. SOX9 Governs Gastric Mucous Neck Cell Identity and Is Required for Injury-Induced Metaplasia. Cell Mol Gastroenterol Hepatol. 2023;16:325-339 pubmed publisher
	Borisova E, Nishimura K, An Y, Takami M, Li J, Song D, et al. Structurally-discovered KLF4 variants accelerate and stabilize reprogramming to pluripotency. iScience. 2022;25:103525 pubmed publisher
	Kant S, Tran K, Kvandová M, Caliz A, Yoo H, Learnard H, et al. PGC1α Regulates the Endothelial Response to Fluid Shear Stress via Telomerase Reverse Transcriptase Control of Heme Oxygenase-1. Arterioscler Thromb Vasc Biol. 2022;42:19-34 pubmed publisher
	Pace J, Bronstein R, Guo Y, Yang Y, Estrada C, Gujarati N, et al. Podocyte-specific KLF4 is required to maintain parietal epithelial cell quiescence in the kidney. Sci Adv. 2021;7:eabg6600 pubmed publisher
	Huang X, Park K, Gontarz P, Zhang B, Pan J, McKenzie Z, et al. OCT4 cooperates with distinct ATP-dependent chromatin remodelers in naïve and primed pluripotent states in human. Nat Commun. 2021;12:5123 pubmed publisher
	Lopez Ramirez M, McCurdy S, Li W, Haynes M, Hale P, Francisco K, et al. Inhibition of the HEG1-KRIT1 interaction increases KLF4 and KLF2 expression in endothelial cells. FASEB Bioadv. 2021;3:334-355 pubmed publisher
	Reddy M, Amaram V, Das S, Tanwar V, Ganguly R, Wang M, et al. lncRNA DRAIR is downregulated in diabetic monocytes and modulates the inflammatory phenotype via epigenetic mechanisms. JCI Insight. 2021;6: pubmed publisher
	Siljee S, Milne B, Brasch H, Bockett N, Patel J, Davis P, et al. Expression of Components of the Renin-Angiotensin System by Cancer Stem Cells in Renal Clear Cell Carcinoma. Biomolecules. 2021;11: pubmed publisher
	Zhao A, Qin H, Sun M, Tang M, Mei J, Ma K, et al. Chemical conversion of human epidermal stem cells into intestinal goblet cells for modeling mucus-microbe interaction and therapy. Sci Adv. 2021;7: pubmed publisher
	V xe9 lot L, Lessard F, B xe9 rub xe9 Simard F, Tav C, Neveu B, Teyssier V, et al. Proximity-dependent Mapping of the Androgen Receptor Identifies Kruppel-like Factor 4 as a Functional Partner. Mol Cell Proteomics. 2021;20:100064 pubmed publisher
	Raschdorf A, S xfc nderhauf A, Skibbe K, Ghebrehiwet B, Peerschke E, Sina C, et al. Heterozygous P32/C1QBP/HABP1 Polymorphism rs56014026 Reduces Mitochondrial Oxidative Phosphorylation and Is Expressed in Low-grade Colorectal Carcinomas. Front Oncol. 2020;10:631592 pubmed publisher
	Sünderhauf A, Raschdorf A, Hicken M, Schlichting H, Fetzer F, Brethack A, et al. GC1qR Cleavage by Caspase-1 Drives Aerobic Glycolysis in Tumor Cells. Front Oncol. 2020;10:575854 pubmed publisher
	Liu W, Mrozek Gorska P, Wirth A, Herold T, Schwarzkopf L, Pich D, et al. Inducible transgene expression in PDX models in vivo identifies KLF4 as a therapeutic target for B-ALL. Biomark Res. 2020;8:46 pubmed publisher
	Kim K, Wu Y, Yoon J, Adachi K, Wu G, Velychko S, et al. Reprogramming competence of OCT factors is determined by transactivation domains. Sci Adv. 2020;6: pubmed publisher
	Chen P, Qin L, Li G, Malagon Lopez J, Wang Z, Bergaya S, et al. Smooth Muscle Cell Reprogramming in Aortic Aneurysms. Cell Stem Cell. 2020;26:542-557.e11 pubmed publisher
	Rong Z, Luo Z, Zhang J, Li T, Zhu Z, Yu Z, et al. GINS complex subunit 4, a prognostic biomarker and reversely mediated by Krüppel-like factor 4, promotes the growth of colorectal cancer. Cancer Sci. 2020;111:1203-1217 pubmed publisher
	Paredes F, Williams H, Quintana R, San Martin A. Mitochondrial Protein Poldip2 Controls VSMC Differentiated Phenotype by O-Linked GlcNAc Transferase-Dependent Inhibition of a Ubiquitin Proteasome System. Circ Res. 2019;: pubmed publisher
	Lin Shiao E, Lan Y, Welzenbach J, Alexander K, Zhang Z, Knapp M, et al. p63 establishes epithelial enhancers at critical craniofacial development genes. Sci Adv. 2019;5:eaaw0946 pubmed publisher
	Pontis J, Planet E, Offner S, Turelli P, Duc J, Coudray A, et al. Hominoid-Specific Transposable Elements and KZFPs Facilitate Human Embryonic Genome Activation and Control Transcription in Naive Human ESCs. Cell Stem Cell. 2019;24:724-735.e5 pubmed publisher
	Wang Q, Vossen A, Ikeda Y, Devaux P. Measles vector as a multigene delivery platform facilitating iPSC reprogramming. Gene Ther. 2019;26:151-164 pubmed publisher
	Chen D, Liu W, Zimmerman J, Pastor W, Kim R, Hosohama L, et al. The TFAP2C-Regulated OCT4 Naive Enhancer Is Involved in Human Germline Formation. Cell Rep. 2018;25:3591-3602.e5 pubmed publisher
	Senís E, Mosteiro L, Wilkening S, Wiedtke E, Nowrouzi A, Afzal S, et al. AAVvector-mediated in vivo reprogramming into pluripotency. Nat Commun. 2018;9:2651 pubmed publisher
	Panatta E, Lena A, Mancini M, Affinati M, Smirnov A, Annicchiarico Petruzzelli M, et al. Kruppel-like factor 4 regulates keratinocyte senescence. Biochem Biophys Res Commun. 2018;499:389-395 pubmed publisher
	Antognelli C, Trapani E, Delle Monache S, Perrelli A, Fornelli C, Retta F, et al. Data in support of sustained upregulation of adaptive redox homeostasis mechanisms caused by KRIT1 loss-of-function. Data Brief. 2018;16:929-938 pubmed publisher
	Wang P, Lan C, Xiong S, Zhao X, Shan Y, Hu R, et al. HIF1α regulates single differentiated glioma cell dedifferentiation to stem-like cell phenotypes with high tumorigenic potential under hypoxia. Oncotarget. 2017;8:28074-28092 pubmed publisher
	Sangwung P, Zhou G, Nayak L, Chan E, Kumar S, Kang D, et al. KLF2 and KLF4 control endothelial identity and vascular integrity. JCI Insight. 2017;2:e91700 pubmed publisher
	Wang P, Wan W, Xiong S, Feng H, Wu N. Cancer stem-like cells can be induced through dedifferentiation under hypoxic conditions in glioma, hepatoma and lung cancer. Cell Death Discov. 2017;3:16105 pubmed publisher

product information

brand :

R&D Systems

master code :

AF3640

SKU :

AF3640

product name :

Human KLF4 Antibody

unit size :

100 ug (also 25 ug)

seo description :

The Human KLF4 Antibody from R&D Systems is a goat polyclonal antibody to KLF4. This antibody reacts with human,mouse,transgenic mouse. The Human KLF4 Antibody has been validated for the following applications: Western Blot,Immunocytochemistry,Mobility Shift Assay,Immunoprecipitation,Immunohistochemistry,Immunohistochemistry-Paraffin,Chromatin Immunoprecipitation (ChIP),Simple Western.

target :

KLF4

category :

Primary Antibodies

buffer :

Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.

clonality :

Polyclonal

concentration :

LYOPH

conjugate :

Unconjugated

dilution :

Western Blot 0.5 ug/mL, Simple Western 25 ug/mL, Immunohistochemistry 15 ug/mL, Chromatin Immunoprecipitation (ChIP) 5 ug/5 x 10^6 cells, Immunocytochemistry 5-15 ug/mL

host :

Goat

immunogen :

E. coli -derived recombinant human KLF4, Ala2-Phe470, Accession # AAH29923

isotype :

IgG

purity :

Antigen Affinity-purified

species :

Human,Mouse,Transgenic Mouse

specificity :

Detects human KLF4 in direct ELISAs and Western blots.

gene symbol :

KLF4

top caption :

Detection of Human KLF4 antibody by Western Blot.

accessionNumbers :

AAH29923

applications :

Simple Western,Mobility Shift Assay,Immunohistochemistry,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Chromatin Immunoprecipitation (ChIP),Immunocytochemistry

USD :

499 USD

alt names :

Epithelial zinc finger protein EZF, EZF, EZFendothelial Kruppel-like zinc finger protein, GKLFKrueppel-like factor 4, Gut-enriched krueppel-like factor, Kruppel-like factor 4 (gut)

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage