Mouse GLI-2 Antibody | R&D Systems AF3635 product information

product summary

company name :

R&D Systems

product type :

antibody

product name :

Mouse GLI-2 Antibody

catalog :

AF3635

quantity :

100 ug (also 25 ug)

price :

509 USD

clonality :

polyclonal

host :

domestic goat

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

R&D Systems product webpage

citations: 31

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; loading ...; fig 3f	Koeniger A, Brichkina A, Nee I, Dempwolff L, Hupfer A, Galperin I, et al. Activation of Cilia-Independent Hedgehog/GLI1 Signaling as a Novel Concept for Neuroblastoma Therapy. Cancers (Basel). 2021;13: pubmed publisher
	U x15b pie x144 ski T, Niewiadomski P. The Proteasome and Cul3-Dependent Protein Ubiquitination Is Required for Gli Protein-Mediated Activation of Gene Expression in the Hedgehog Pathway. Cells. 2024;13: pubmed publisher
	Baran B, Derua R, Janssens V, Niewiadomski P. PP2A phosphatase regulatory subunit PPP2R3C is a new positive regulator of the hedgehog signaling pathway. Cell Signal. 2024;123:111352 pubmed publisher
	Shi P, Tian J, Mallinger J, Ling D, Deleyrolle L, McIntyre J, et al. Increasing Ciliary ARL13B Expression Drives Active and Inhibitor-Resistant Smoothened and GLI into Glioma Primary Cilia. Cells. 2023;12: pubmed publisher
	Bagka M, Choi H, H xe9 ritier M, Schwaemmle H, Pasquer Q, Braun S, et al. Targeted protein degradation reveals BET bromodomains as the cellular target of Hedgehog pathway inhibitor-1. Nat Commun. 2023;14:3893 pubmed publisher
	Yang F, Rodriguez Blanco J, Long J, Swiderska Syn M, Wynn D, Li B, et al. A Druggable UHRF1/DNMT1/GLI Complex Regulates Sonic Hedgehog-Dependent Tumor Growth. Mol Cancer Res. 2022;20:1598-1610 pubmed publisher
	Sun J, Yoon J, Lee M, Lee H, Hwang Y, Daar I. Zic5 stabilizes Gli3 via a non-transcriptional mechanism during retinal development. Cell Rep. 2022;38:110312 pubmed publisher
	Hwang S, Somatilaka B, White K, Mukhopadhyay S. Ciliary and extraciliary Gpr161 pools repress hedgehog signaling in a tissue-specific manner. elife. 2021;10: pubmed publisher
	Peng H, Zhang J, Ya A, Ma W, Villa S, Sukenik S, et al. Myomegalin regulates Hedgehog pathway by controlling PDE4D at the centrosome. Mol Biol Cell. 2021;32:1807-1817 pubmed publisher
	Wasson C, Ross R, Wells R, Corinaldesi C, Georgiou I, Riobo Del Galdo N, et al. Long non-coding RNA HOTAIR induces GLI2 expression through Notch signalling in systemic sclerosis dermal fibroblasts. Arthritis Res Ther. 2020;22:286 pubmed publisher
	Dias J, Alekseenko Z, Jeggari A, Boareto M, Vollmer J, Kozhevnikova M, et al. A Shh/Gli-driven three-node timer motif controls temporal identity and fate of neural stem cells. Sci Adv. 2020;6: pubmed publisher
	Yoshida S, Aoki K, Fujiwara K, Nakakura T, Kawamura A, Yamada K, et al. The novel ciliogenesis regulator DYRK2 governs Hedgehog signaling during mouse embryogenesis. elife. 2020;9: pubmed publisher
	Somatilaka B, Hwang S, Palicharla V, White K, Badgandi H, Shelton J, et al. Ankmy2 Prevents Smoothened-Independent Hyperactivation of the Hedgehog Pathway via Cilia-Regulated Adenylyl Cyclase Signaling. Dev Cell. 2020;: pubmed publisher
	Hom M, Ondrus A, Sakata Kato T, Rack P, Chen J. Bicyclic Imidazolium Inhibitors of Gli Transcription Factor Activity. ChemMedChem. 2020;15:1044-1049 pubmed publisher
	Constable S, Long A, Floyd K, Schurmans S, Caspary T. The ciliary phosphatidylinositol phosphatase Inpp5e plays positive and negative regulatory roles in Shh signaling. Development. 2020;147: pubmed publisher
	Lospinoso Severini L, Quaglio D, Basili I, Ghirga F, Bufalieri F, Caimano M, et al. A Smo/Gli Multitarget Hedgehog Pathway Inhibitor Impairs Tumor Growth. Cancers (Basel). 2019;11: pubmed publisher
	Martin Hurtado A, Martin Morales R, Robledinos Antón N, Blanco R, Palacios Blanco I, Lastres Becker I, et al. NRF2-dependent gene expression promotes ciliogenesis and Hedgehog signaling. Sci Rep. 2019;9:13896 pubmed publisher
	Bufalieri F, Infante P, Bernardi F, Caimano M, Romania P, Moretti M, et al. ERAP1 promotes Hedgehog-dependent tumorigenesis by controlling USP47-mediated degradation of βTrCP. Nat Commun. 2019;10:3304 pubmed publisher
	Ma P, Song N, Li Y, Zhang Q, Zhang L, Zhang L, et al. Fine-Tuning of Shh/Gli Signaling Gradient by Non-proteolytic Ubiquitination during Neural Patterning. Cell Rep. 2019;28:541-553.e4 pubmed publisher
	Han Y, Wang B, Cho Y, Zhu J, Wu J, Chen Y, et al. Phosphorylation of Ci/Gli by Fused Family Kinases Promotes Hedgehog Signaling. Dev Cell. 2019;50:610-626.e4 pubmed publisher
	Wojcinski A, Morabito M, Lawton A, Stephen D, Joyner A. Genetic deletion of genes in the cerebellar rhombic lip lineage can stimulate compensation through adaptive reprogramming of ventricular zone-derived progenitors. Neural Dev. 2019;14:4 pubmed publisher
	Massa F, Tammaro R, Prado M, Cesana M, Lee B, Finley D, et al. The deubiquitinating enzyme Usp14 controls ciliogenesis and Hedgehog signaling. Hum Mol Genet. 2019;28:764-777 pubmed publisher
	Infante P, Faedda R, Bernardi F, Bufalieri F, Lospinoso Severini L, Alfonsi R, et al. Itch/β-arrestin2-dependent non-proteolytic ubiquitylation of SuFu controls Hedgehog signalling and medulloblastoma tumorigenesis. Nat Commun. 2018;9:976 pubmed publisher
	Rosengren T, Larsen L, Pedersen L, Christensen S, Møller L. TSC1 and TSC2 regulate cilia length and canonical Hedgehog signaling via different mechanisms. Cell Mol Life Sci. 2018;75:2663-2680 pubmed publisher
	Guen V, Chavarria T, Kröger C, Ye X, Weinberg R, Lees J. EMT programs promote basal mammary stem cell and tumor-initiating cell stemness by inducing primary ciliogenesis and Hedgehog signaling. Proc Natl Acad Sci U S A. 2017;114:E10532-E10539 pubmed publisher
	Li J, Cui Y, Xu J, Wang Q, Yang X, Li Y, et al. Suppressor of Fused restraint of Hedgehog activity level is critical for osteogenic proliferation and differentiation during calvarial bone development. J Biol Chem. 2017;292:15814-15825 pubmed publisher
	Han Y, Xiong Y, Shi X, Wu J, Zhao Y, Jiang J. Regulation of Gli ciliary localization and Hedgehog signaling by the PY-NLS/karyopherin-?2 nuclear import system. PLoS Biol. 2017;15:e2002063 pubmed publisher
	Hoelzl M, Heby Henricson K, Gerling M, Dias J, Kuiper R, Trünkle C, et al. Differential requirement of SUFU in tissue development discovered in a hypomorphic mouse model. Dev Biol. 2017;429:132-146 pubmed publisher
	Cai H, Liu A. Spop regulates Gli3 activity and Shh signaling in dorsoventral patterning of the mouse spinal cord. Dev Biol. 2017;432:72-85 pubmed publisher
	Cai H, Liu A. Spop promotes skeletal development and homeostasis by positively regulating Ihh signaling. Proc Natl Acad Sci U S A. 2016;113:14751-14756 pubmed publisher
	Torrado B, Grana M, Badano J, Irigoín F. Ciliary Entry of the Hedgehog Transcriptional Activator Gli2 Is Mediated by the Nuclear Import Machinery but Differs from Nuclear Transport in Being Imp-α/β1-Independent. PLoS ONE. 2016;11:e0162033 pubmed publisher

product information

master code :

AF3635

SKU :

AF3635

product name :

Mouse GLI-2 Antibody

unit size :

100 ug (also 25 ug)

description :

The Mouse GLI-2 Antibody from R&D Systems is a goat polyclonal antibody to GLI-2. This antibody reacts with drosophila,human,mouse,transgenic mouse,frog - xenopus (african clawed frog). The Mouse GLI-2 Antibody has been validated for the following applications: Western Blot,Immunocytochemistry,Immunocytochemistry/Immunofluorescence,Immunoprecipitation,Chromatin Immunoprecipitation,IF/ICC,Immunohistochemistry-Paraffin,Immunohistochemistry.

target :

GLI-2

category :

Primary Antibodies

buffer :

Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.

clonality :

Polyclonal

concentration :

LYOPH

conjugate :

Unconjugated

host :

Goat

immunogen :

E. coli -derived recombinant mouse GLI-2, Met1-Glu416, Accession # NP_001074594

isotype :

IgG

purity :

Antigen Affinity-purified

species :

Human,Mouse,Transgenic Mouse,Frog - Xenopus (African Clawed Frog)

specificity :

Detects mouse GLI-2 in direct ELISAs and Western blots.

gene symbol :

GLI2

top caption :

GLI-2 antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr).

accessionNumbers :

NP_001074594

applications :

Immunoprecipitation,Western Blot,Chromatin Immunoprecipitation,Immunocytochemistry,IF/ICC,Immunohistochemistry-Paraffin,Immunohistochemistry

USD :

509 USD

alt names :

GLI family zinc finger 2, GLI2, GLI-Kruppel family member GLI2, glioma-associated oncogene family zinc finger 2, HPE9tax helper protein 1, oncogene GLI2, Tax helper protein, tax helper protein 2, tax-responsive element-2 holding protein, tax-responsive element-25-bp sequence binding protein, THP, THP1, THP2, zinc finger protein GLI2

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage