product summary
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company name :
R&D Systems
product type :
antibody
product name :
Human IL-33 Antibody
catalog :
AF3625
quantity :
100 ug (also 25 ug)
price :
519 USD
clonality :
polyclonal
host :
domestic goat
conjugate :
nonconjugated
reactivity :
human, mouse
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, neutralization, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
R&D Systems product webpage
citations: 32
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; human; 1:400; loading ...; fig 4a
Ercolano G, Gomez Cadena A, Dumauthioz N, Vanoni G, Kreutzfeldt M, Wyss T, et al. PPARɣ drives IL-33-dependent ILC2 pro-tumoral functions. Nat Commun. 2021;12:2538 pubmed publisher
Donahue K, Watkoske H, Kadiyala P, Du W, Brown K, Scales M, et al. Oncogenic KRAS-Dependent Stromal Interleukin-33 Directs the Pancreatic Microenvironment to Promote Tumor Growth. Cancer Discov. 2024;14:1964-1989 pubmed publisher
Jeon Y, Gil C, Won J, Jo A, Kim H. Symbiotic microbiome Staphylococcus epidermidis restricts IL-33 production in allergic nasal epithelium via limiting the cellular necroptosis. BMC Microbiol. 2023;23:154 pubmed publisher
Stephenson K, Porte J, Kelly A, Wallace W, Huntington C, Overed Sayer C, et al. The IL-33:ST2 axis is unlikely to play a central fibrogenic role in idiopathic pulmonary fibrosis. Respir Res. 2023;24:89 pubmed publisher
Yao F, Luo Y, Liu Y, Chen Y, Li Y, Hu X, et al. Imatinib inhibits pericyte-fibroblast transition and inflammation and promotes axon regeneration by blocking the PDGF-BB/PDGFRβ pathway in spinal cord injury. Inflamm Regen. 2022;42:44 pubmed publisher
Jowett G, Read E, Roberts L, Coman D, Vil xe0 Gonz xe1 lez M, Zabinski T, et al. Organoids capture tissue-specific innate lymphoid cell development in mice and humans. Cell Rep. 2022;40:111281 pubmed publisher
Bahri R, Kiss O, Prise I, Garcia Rodriguez K, Atmoko H, Mart xed nez G xf3 mez J, et al. Human Melanoma-Associated Mast Cells Display a Distinct Transcriptional Signature Characterized by an Upregulation of the Complement Component 3 That Correlates With Poor Prognosis. Front Immunol. 2022;13:861545 pubmed publisher
Alam A, Levanduski E, Denz P, Villavicencio H, Bhatta M, Alhorebi L, et al. Fungal mycobiome drives IL-33 secretion and type 2 immunity in pancreatic cancer. Cancer Cell. 2022;40:153-167.e11 pubmed publisher
Tsuda H, Tominaga S, Ohtsuki M, Komine M. Nuclear IL-33 regulates cytokinesis and cell motility in normal human epidermal keratinocytes. J Dermatol Sci. 2022;105:113-120 pubmed publisher
Meng X, Qian X, Ding X, Wang W, Yin X, Zhuang G, et al. Eosinophils regulate intra-adipose axonal plasticity. Proc Natl Acad Sci U S A. 2022;119: pubmed publisher
Miller J, Lingegowda H, Symons L, Bougie O, Young S, Lessey B, et al. IL-33 activates group 2 innate lymphoid cell expansion and modulates endometriosis. JCI Insight. 2021;6: pubmed publisher
Mehrara B, Park H, Kataru R, Bromberg J, Coriddi M, Baik J, et al. Pilot Study of Anti-Th2 Immunotherapy for the Treatment of Breast Cancer-Related Upper Extremity Lymphedema. Biology (Basel). 2021;10: pubmed publisher
Mikuls T, Gaurav R, Thiele G, England B, Wolfe M, Shaw B, et al. The impact of airborne endotoxin exposure on rheumatoid arthritis-related joint damage, autoantigen expression, autoimmunity, and lung disease. Int Immunopharmacol. 2021;100:108069 pubmed publisher
Gaurav R, Anderson D, Radio S, BAILEY K, England B, Mikuls T, et al. IL-33 Depletion in COVID-19 Lungs. Chest. 2021;160:1656-1659 pubmed publisher
Perez F, Ruera C, Miculán E, Carasi P, Dubois Camacho K, Garbi L, et al. IL-33 Alarmin and Its Active Proinflammatory Fragments Are Released in Small Intestine in Celiac Disease. Front Immunol. 2020;11:581445 pubmed publisher
Yangngam S, Thongchot S, Pongpaibul A, Vaeteewoottacharn K, Pinlaor S, Thuwajit P, et al. High level of interleukin-33 in cancer cells and cancer-associated fibroblasts correlates with good prognosis and suppressed migration in cholangiocarcinoma. J Cancer. 2020;11:6571-6581 pubmed publisher
Jeon Y, Gil C, Won J, Jo A, Kim H. Symbiotic microbiome Staphylococcus aureus from human nasal mucus modulates IL-33-mediated type 2 immune responses in allergic nasal mucosa. BMC Microbiol. 2020;20:301 pubmed publisher
Shani O, Vorobyov T, Monteran L, Lavie D, Cohen N, Raz Y, et al. Fibroblast-Derived IL33 Facilitates Breast Cancer Metastasis by Modifying the Immune Microenvironment and Driving Type 2 Immunity. Cancer Res. 2020;80:5317-5329 pubmed publisher
Lu Y, Basatemur G, Scott I, Chiarugi D, Clement M, Harrison J, et al. Interleukin-33 Signaling Controls the Development of Iron-Recycling Macrophages. Immunity. 2020;52:782-793.e5 pubmed publisher
Govatati S, Pichavaram P, Janjanam J, Guo L, Virmani R, Rao G. Myristoylation of LMCD1 Leads to Its Species-Specific Derepression of E2F1 and NFATc1 in the Modulation of CDC6 and IL-33 Expression During Development of Vascular Lesions. Arterioscler Thromb Vasc Biol. 2020;40:1256-1274 pubmed publisher
Moral J, Leung J, Rojas L, Ruan J, Zhao J, Sethna Z, et al. ILC2s amplify PD-1 blockade by activating tissue-specific cancer immunity. Nature. 2020;579:130-135 pubmed publisher
Zhang J, Tao T, Wang K, Zhang G, Yan Y, Lin H, et al. IL-33/ST2 axis promotes glioblastoma cell invasion by accumulating tenascin-C. Sci Rep. 2019;9:20276 pubmed publisher
Izadi D, Layton T, Williams L, McCann F, Cabrita M, Espirito Santo A, et al. Identification of TNFR2 and IL-33 as therapeutic targets in localized fibrosis. Sci Adv. 2019;5:eaay0370 pubmed publisher
Zhou Q, Wu X, Wang X, Yu Z, Pan T, Li Z, et al. The reciprocal interaction between tumor cells and activated fibroblasts mediated by TNF-α/IL-33/ST2L signaling promotes gastric cancer metastasis. Oncogene. 2019;: pubmed publisher
Landskron G, De la Fuente López M, Dubois Camacho K, Díaz Jiménez D, Orellana Serradell O, Romero D, et al. Interleukin 33/ST2 Axis Components Are Associated to Desmoplasia, a Metastasis-Related Factor in Colorectal Cancer. Front Immunol. 2019;10:1394 pubmed publisher
Frisbee A, Saleh M, Young M, Leslie J, Simpson M, Abhyankar M, et al. IL-33 drives group 2 innate lymphoid cell-mediated protection during Clostridium difficile infection. Nat Commun. 2019;10:2712 pubmed publisher
Govatati S, Pichavaram P, Janjanam J, Zhang B, Singh N, Mani A, et al. NFATc1-E2F1-LMCD1-Mediated IL-33 Expression by Thrombin Is Required for Injury-Induced Neointima Formation. Arterioscler Thromb Vasc Biol. 2019;39:1212-1226 pubmed publisher
Razumilava N, Shiota J, Mohamad Zaki N, Ocadiz Ruiz R, Cieslak C, Zakharia K, et al. Hedgehog Signaling Modulates Interleukin-33-Dependent Extrahepatic Bile Duct Cell Proliferation in Mice. Hepatol Commun. 2019;3:277-292 pubmed publisher
Cui G, Ren J, Xu G, Li Z, Zheng W, Yuan A. Cellular and clinicopathological features of the IL-33/ST2 axis in human esophageal squamous cell carcinomas. Cancer Cell Int. 2018;18:203 pubmed publisher
Gour N, Lajoie S, Smole U, White M, Hu D, Goddard P, et al. Dysregulated invertebrate tropomyosin-dectin-1 interaction confers susceptibility to allergic diseases. Sci Immunol. 2018;3: pubmed publisher
Lapérine O, Cloitre A, Caillon J, Huck O, Bugueno I, Pilet P, et al. Interleukin-33 and RANK-L Interplay in the Alveolar Bone Loss Associated to Periodontitis. PLoS ONE. 2016;11:e0168080 pubmed publisher
Ganesan S, Pham D, Jing Y, Farazuddin M, Hudy M, Unger B, et al. TLR2 Activation Limits Rhinovirus-Stimulated CXCL-10 by Attenuating IRAK-1-Dependent IL-33 Receptor Signaling in Human Bronchial Epithelial Cells. J Immunol. 2016;197:2409-20 pubmed publisher
product information
brand :
R&D Systems
master code :
AF3625
SKU :
AF3625
product name :
Human IL-33 Antibody
unit size :
100 ug (also 25 ug)
seo description :
The Human IL-33 Antibody from R&D Systems is a goat polyclonal antibody to IL-33. This antibody reacts with human,mouse,transgenic mouse. The Human IL-33 Antibody has been validated for the following applications: Immunohistochemistry,Western Blot,ELISA Development,Neutralization,Cell Culture,ELISA Capture,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunofluorescence,Immunocytochemistry,ELISA Capture (Matched Antibody Pair),Dual RNAscope ISH-IHC Compatible.
target :
IL-33
category :
Primary Antibodies
buffer :
Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.
clonality :
Polyclonal
concentration :
LYOPH
conjugate :
Unconjugated
dilution :
Western Blot 0.1 ug/mL, Immunohistochemistry 5-15 ug/mL, Neutralization 0.75-3.0 ug/mL, Immunocytochemistry 5-15 ug/mL, Dual RNAscope ISH-IHC 3-25 ug/mL, ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
host :
Goat
immunogen :
E. coli -derived recombinant human IL-33, Ser112-Thr270, Accession # O95760
isotype :
IgG
purity :
Antigen Affinity-purified
species :
Human,Mouse,Transgenic Mouse
specificity :
Detects human IL-33 in ELISAs and Western blots. In sandwich ELISAs, less than 0.05% cross-reactivity with recombinant mouse (rm) IL-33 is observed.
gene symbol :
IL33
endotoxin note :
<0.10 EU per 1 ╡g of the antibody by the LAL method.
Antibody validation :
Orthogonal Validation
top caption :
Cell Proliferation Induced by IL-33 and Neutralization by Human IL-33 Antibody.
accessionNumbers :
O95760
applications :
ELISA Capture (Matched Antibody Pair),Dual RNAscope ISH-IHC Compatible,ELISA Development,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Neutralization,Immunofluorescence,Immunocytochemistry,Cell Culture,ELISA Capture
USD :
519 USD
alt names :
C9orf26, C9orf26chromosome 9 open reading frame 26 (NF-HEV), DKFZp586H0523, DVS27, DVS27-related protein, IL1F11, IL-1F11, IL33, IL-33, interleukin 33, Interleukin-1 family member 11, interleukin-33, NFHEV, NF-HEV, NF-HEVNFEHEV, Nuclear factor from high endothelial venules, RP11-575C20.2
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.
more info or order :
R&D Systems product webpage
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.