Human VEGFR2/KDR/Flk-1 Antibody | R&D Systems AF357 product information

product summary

company name :

R&D Systems

product type :

antibody

product name :

Human VEGFR2/KDR/Flk-1 Antibody

catalog :

AF357

quantity :

100 ug (also 25 ug)

price :

489 USD

clonality :

polyclonal

host :

domestic goat

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, neutralization, immunoprecipitation, immunohistochemistry - paraffin section, proximity ligation assay

more info or order :

R&D Systems product webpage

citations: 31

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; human; fig 4c	Veschi V, Mangiapane L, Nicotra A, Di Franco S, Scavo E, Apuzzo T, et al. Targeting chemoresistant colorectal cancer via systemic administration of a BMP7 variant. Oncogene. 2020;39:987-1003 pubmed publisher
proximity ligation assay; human; 1:100; loading ...; fig 4b	Morin E, Sjöberg E, Tjomsland V, Testini C, Lindskog C, Franklin O, et al. VEGF receptor-2/neuropilin 1 trans-complex formation between endothelial and tumor cells is an independent predictor of pancreatic cancer survival. J Pathol. 2018;246:311-322 pubmed publisher
immunoprecipitation; human; fig 3 immunocytochemistry; human; fig 2 western blot; human; fig 1	Fearnley G, Smith G, Abdul Zani I, Yuldasheva N, Mughal N, Homer Vanniasinkam S, et al. VEGF-A isoforms program differential VEGFR2 signal transduction, trafficking and proteolysis. Biol Open. 2016;5:571-83 pubmed publisher
	Essebier P, Keyser M, Yordanov T, Hill B, Yu A, Noordstra I, et al. c-Src-induced vascular malformations require localised matrix degradation at focal adhesions. J Cell Sci. 2024;137: pubmed publisher
	Xing L, Huang G, Chen R, Huang L, Liu J, Ren X, et al. Critical role of mitogen-inducible gene 6 in restraining endothelial cell permeability to maintain vascular homeostasis. J Cell Commun Signal. 2023;17:151-165 pubmed publisher
	Nakashima Y, Miyagi Shiohira C, Saitoh I, Watanabe M, Matsushita M, Tsukahara M, et al. Induced hepatic stem cells are suitable for human hepatocyte production. iScience. 2022;25:105052 pubmed publisher
	Straehla J, Hajal C, Safford H, Offeddu G, Boehnke N, Dacoba T, et al. A predictive microfluidic model of human glioblastoma to assess trafficking of blood-brain barrier-penetrant nanoparticles. Proc Natl Acad Sci U S A. 2022;119:e2118697119 pubmed publisher
	Cano I, Hu Z, AbuSamra D, Saint Geniez M, Ng Y, Arg xfc eso P, et al. Galectin-3 Enhances Vascular Endothelial Growth Factor-A Receptor 2 Activity in the Presence of Vascular Endothelial Growth Factor. Front Cell Dev Biol. 2021;9:734346 pubmed publisher
	Ash D, Sudhahar V, Youn S, Okur M, Das A, O Bryan J, et al. The P-type ATPase transporter ATP7A promotes angiogenesis by limiting autophagic degradation of VEGFR2. Nat Commun. 2021;12:3091 pubmed publisher
	Kempers L, Wakayama Y, van der Bijl I, Furumaya C, De Cuyper I, Jongejan A, et al. The endosomal RIN2/Rab5C machinery prevents VEGFR2 degradation to control gene expression and tip cell identity during angiogenesis. Angiogenesis. 2021;24:695-714 pubmed publisher
	Dallinga M, Habani Y, Schimmel A, Dallinga Thie G, Van Noorden C, Klaassen I, et al. The Role of Heparan Sulfate and Neuropilin 2 in VEGFA Signaling in Human Endothelial Tip Cells and Non-Tip Cells during Angiogenesis In Vitro. Cells. 2021;10: pubmed publisher
	Burger S, Meng J, Zwanzig A, Beck M, Pankonin M, Wiedemann P, et al. Pigment Epithelium-Derived Factor (PEDF) Receptors Are Involved in Survival of Retinal Neurons. Int J Mol Sci. 2020;22: pubmed publisher
	Nitzsche A, Pietilä R, Love D, Testini C, Ninchoji T, Smith R, et al. Paladin is a phosphoinositide phosphatase regulating endosomal VEGFR2 signalling and angiogenesis. EMBO Rep. 2021;22:e50218 pubmed publisher
	Hu Z, Cano I, Saez Torres K, LeBlanc M, Saint Geniez M, Ng Y, et al. Elements of the Endomucin Extracellular Domain Essential for VEGF-Induced VEGFR2 Activity. Cells. 2020;9: pubmed publisher
	Caolo V, Debant M, Endesh N, Futers T, Lichtenstein L, Bartoli F, et al. Shear stress activates ADAM10 sheddase to regulate Notch1 via the Piezo1 force sensor in endothelial cells. elife. 2020;9: pubmed publisher
	Wei S, Huang Y, Hsieh M, Lin Y, Shyu W, Chen H, et al. Atypical chemokine receptor ACKR3/CXCR7 controls postnatal vasculogenesis and arterial specification by mesenchymal stem cells via Notch signaling. Cell Death Dis. 2020;11:307 pubmed publisher
	Morin E, Lindskog C, Johansson M, Egevad L, Sandstrom P, Harmenberg U, et al. Perivascular Neuropilin-1 expression is an independent marker of improved survival in renal cell carcinoma. J Pathol. 2020;250:387-396 pubmed publisher
	Fearnley G, Latham A, Hollstein M, Odell A, Ponnambalam S. ATF-2 and Tpl2 regulation of endothelial cell cycle progression and apoptosis. Cell Signal. 2020;66:109481 pubmed publisher
	Hickman R, Leeman Neill R, Rosenblum M, Anderson R, Goldman J. Co-existent pilocytic astrocytoma with acute B-cell leukemia within the cerebellum. Neuropathology. 2019;39:394-397 pubmed publisher
	Cossutta M, Darche M, Carpentier G, Houppe C, Ponzo M, Raineri F, et al. Weibel-Palade Bodies Orchestrate Pericytes During Angiogenesis. Arterioscler Thromb Vasc Biol. 2019;39:1843-1858 pubmed publisher
	LeBlanc M, Saez Torres K, Cano I, Hu Z, Saint Geniez M, Ng Y, et al. Glycocalyx regulation of vascular endothelial growth factor receptor 2 activity. FASEB J. 2019;:fj201900011R pubmed publisher
	Genet G, Boyé K, Mathivet T, Ola R, Zhang F, Dubrac A, et al. Endophilin-A2 dependent VEGFR2 endocytosis promotes sprouting angiogenesis. Nat Commun. 2019;10:2350 pubmed publisher
	Jha S, Rauniyar K, Chronowska E, Mattonet K, Maina E, Koistinen H, et al. KLK3/PSA and cathepsin D activate VEGF-C and VEGF-D. elife. 2019;8: pubmed publisher
	Fearnley G, Abdul Zani I, Latham A, Hollstein M, Ladbury J, Wheatcroft S, et al. Tpl2 is required for VEGF-A-stimulated signal transduction and endothelial cell function. Biol Open. 2019;8: pubmed publisher
	Blanchard T, Czinn S, Banerjee V, Sharda N, Bafford A, Mubariz F, et al. Identification of Cross Talk between FoxM1 and RASSF1A as a Therapeutic Target of Colon Cancer. Cancers (Basel). 2019;11: pubmed publisher
	Tiede C, Bedford R, Heseltine S, Smith G, Wijetunga I, Ross R, et al. Affimer proteins are versatile and renewable affinity reagents. elife. 2017;6: pubmed publisher
	Volk Draper L, Hall K, Wilber A, Ran S. Lymphatic endothelial progenitors originate from plastic myeloid cells activated by toll-like receptor-4. PLoS ONE. 2017;12:e0179257 pubmed publisher
	Jin Y, Muhl L, Burmakin M, Wang Y, Duchez A, Betsholtz C, et al. Endoglin prevents vascular malformation by regulating flow-induced cell migration and specification through VEGFR2 signalling. Nat Cell Biol. 2017;19:639-652 pubmed publisher
	Heinolainen K, Karaman S, D Amico G, Tammela T, Sormunen R, Eklund L, et al. VEGFR3 Modulates Vascular Permeability by Controlling VEGF/VEGFR2 Signaling. Circ Res. 2017;120:1414-1425 pubmed publisher
	Alghanem A, Wilkinson E, Emmett M, Aljasir M, Holmes K, Rothermel B, et al. RCAN1.4 regulates VEGFR-2 internalisation, cell polarity and migration in human microvascular endothelial cells. Angiogenesis. 2017;20:341-358 pubmed publisher
	Basagiannis D, Zografou S, Murphy C, Fotsis T, Morbidelli L, Ziche M, et al. VEGF induces signalling and angiogenesis by directing VEGFR2 internalisation through macropinocytosis. J Cell Sci. 2016;129:4091-4104 pubmed

product information

master code :

AF357

SKU :

AF357

product name :

Human VEGFR2/KDR/Flk-1 Antibody

unit size :

100 ug (also 25 ug)

description :

The Human VEGFR2/KDR/Flk-1 Antibody from R&D Systems is a goat polyclonal antibody to VEGFR2/KDR/Flk-1. This antibody reacts with human,mouse,primate - papio anubis (olive baboon),rat,xenograft. The Human VEGFR2/KDR/Flk-1 Antibody has been validated for the following applications: Immunohistochemistry,Neutralization,Immunocytochemistry,Immunoprecipitation,Western Blot,Functional Assay,Immunohistochemistry-Paraffin.

target :

VEGFR2/KDR/Flk-1

category :

Primary Antibodies

buffer :

Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.

clonality :

Polyclonal

concentration :

LYOPH

conjugate :

Unconjugated

host :

Goat

immunogen :

Mouse myeloma cell line NS0-derived recombinant human VEGFR2/KDR/Flk-1, Ala20-Glu764, Accession # AAC16450

isotype :

IgG

purity :

Antigen Affinity-purified

species :

Human,Mouse,Primate - Papio anubis (Olive Baboon),Rat,Xenograft

specificity :

Detects human VEGFR2/KDR/Flk-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant mouse VEGFR2 is observed and less than 10% cross-reactivity with recombinant human (rh) VEGFR1 and rhVEGFR3 is observed.

gene symbol :

KDR

endotoxin note :

<0.10 EU per 1 ╡g of the antibody by the LAL method.

top caption :

Detection of Human VEGFR2/KDR/Flk-1 antibody by Western Blot.

accessionNumbers :

AAC16450

applications :

Immunoprecipitation,Neutralization,Western Blot,Functional Assay,Immunohistochemistry,Immunohistochemistry-Paraffin,Immunocytochemistry

USD :

489 USD

alt names :

CD309, CD309 antigen, EC 2.7.10, EC 2.7.10.1, Fetal liver kinase 1, fetal liver kinase-1, Flk1, Flk-1, FLK1tyrosine kinase growth factor receptor, KDR, Kinase insert domain receptor, kinase insert domain receptor (a type III receptor tyrosine kinase), KRD1, Ly73, Protein-tyrosine kinase receptor flk-1, soluble VEGFR2, vascular endothelial growth factor receptor 2, VEGF R2, VEGFR, VEGFR2, VEGFR-2

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage