product summary
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company name :
R&D Systems
product type :
antibody
product name :
Human Neurogenin-3 Antibody
catalog :
AF3444
quantity :
100 ug (also 25 ug)
price :
499 USD
clonality :
polyclonal
host :
domestic sheep
conjugate :
nonconjugated
reactivity :
human, mouse
application :
western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
more info or order :
R&D Systems product webpage
citations: 14
Reference
Jim xe9 nez S, Schreiber V, Mercier R, Gradwohl G, Molina N. Characterization of cell-fate decision landscapes by estimating transcription factor dynamics. Cell Rep Methods. 2023;3:100512 pubmed publisher
Miguel Escalada I, Maestro M, Balboa D, Elek A, Bernal A, Bernardo E, et al. Pancreas agenesis mutations disrupt a lead enhancer controlling a developmental enhancer cluster. Dev Cell. 2022;57:1922-1936.e9 pubmed publisher
Yong H, Xie G, Liu C, Wang W, Naji A, Irianto J, et al. Gene Signatures of NEUROGENIN3+ Endocrine Progenitor Cells in the Human Pancreas. Front Endocrinol (Lausanne). 2021;12:736286 pubmed publisher
Hogrebe N, Maxwell K, Augsornworawat P, Millman J. Generation of insulin-producing pancreatic β cells from multiple human stem cell lines. Nat Protoc. 2021;16:4109-4143 pubmed publisher
Velazco Cruz L, Goedegebuure M, Maxwell K, Augsornworawat P, Hogrebe N, Millman J. SIX2 Regulates Human β Cell Differentiation from Stem Cells and Functional Maturation In Vitro. Cell Rep. 2020;31:107687 pubmed publisher
Vinckier N, Patel N, Geusz R, Wang A, Wang J, Matta I, et al. LSD1-mediated enhancer silencing attenuates retinoic acid signalling during pancreatic endocrine cell development. Nat Commun. 2020;11:2082 pubmed publisher
Hogrebe N, Augsornworawat P, Maxwell K, Velazco Cruz L, Millman J. Targeting the cytoskeleton to direct pancreatic differentiation of human pluripotent stem cells. Nat Biotechnol. 2020;38:460-470 pubmed publisher
Alvarez Dominguez J, Donaghey J, Rasouli N, Kenty J, Helman A, Charlton J, et al. Circadian Entrainment Triggers Maturation of Human In Vitro Islets. Cell Stem Cell. 2020;26:108-122.e10 pubmed publisher
Sharon N, Vanderhooft J, Straubhaar J, Mueller J, Chawla R, Zhou Q, et al. Wnt Signaling Separates the Progenitor and Endocrine Compartments during Pancreas Development. Cell Rep. 2019;27:2281-2291.e5 pubmed publisher
Rosado Olivieri E, Anderson K, Kenty J, Melton D. YAP inhibition enhances the differentiation of functional stem cell-derived insulin-producing β cells. Nat Commun. 2019;10:1464 pubmed publisher
Ramond C, Beydag Tasöz B, Azad A, van de Bunt M, Petersen M, Beer N, et al. Understanding human fetal pancreas development using subpopulation sorting, RNA sequencing and single-cell profiling. Development. 2018;145: pubmed publisher
Aigha I, Memon B, Elsayed A, Abdelalim E. Differentiation of human pluripotent stem cells into two distinct NKX6.1 populations of pancreatic progenitors. Stem Cell Res Ther. 2018;9:83 pubmed publisher
Krentz N, Van Hoof D, Li Z, Watanabe A, Tang M, Nian C, et al. Phosphorylation of NEUROG3 Links Endocrine Differentiation to the Cell Cycle in Pancreatic Progenitors. Dev Cell. 2017;41:129-142.e6 pubmed publisher
Saarimäki Vire J, Balboa D, Russell M, Saarikettu J, Kinnunen M, Keskitalo S, et al. An Activating STAT3 Mutation Causes Neonatal Diabetes through Premature Induction of Pancreatic Differentiation. Cell Rep. 2017;19:281-294 pubmed publisher
product information
brand :
R&D Systems
master code :
AF3444
SKU :
AF3444
product name :
Human Neurogenin-3 Antibody
unit size :
100 ug (also 25 ug)
seo description :
The Human Neurogenin-3 Antibody from R&D Systems is a sheep polyclonal antibody to Neurogenin-3. This antibody reacts with human,mouse. The Human Neurogenin-3 Antibody has been validated for the following applications: Flow Cytometry,Western Blot,Immunohistochemistry-Paraffin,Immunocytochemistry,Immunohistochemistry.
target :
Neurogenin-3
category :
Primary Antibodies
buffer :
Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.
clonality :
Polyclonal
concentration :
LYOPH
conjugate :
Unconjugated
dilution :
Western Blot 1 ug/mL, Immunohistochemistry 1-15 ug/mL
host :
Sheep
immunogen :
E. coli -derived recombinant human Neurogenin-3, Met1-Leu214, Accession # Q9Y4Z2
isotype :
IgG
purity :
Antigen Affinity-purified
species :
Human,Mouse
specificity :
Detects human Neurogenin-3 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse Neurogenin-3 is observed, and less than 1% cross-reactivity with recombinant human Neurogenin-1 is observed.
gene symbol :
NEUROG3
top caption :
Detection of Human Neurogenin-3 antibody by Western Blot.
accessionNumbers :
Q9Y4Z2
applications :
Immunohistochemistry,Flow Cytometry,Western Blot,Immunohistochemistry-Paraffin,Immunocytochemistry
USD :
499 USD
alt names :
Atoh5, BHLHA7, bHLHa7NGN-3, Class A basic helix-loop-helix protein 7, Math4B, NEUROG3, neurogenin 3, neurogenin-3, Neurogenin3, NGN3, Protein atonal homolog 5, Relax
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.
more info or order :
R&D Systems product webpage
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.