product summary
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company name :
R&D Systems
product type :
antibody
product name :
Human VEGFR1/Flt-1 Antibody
catalog :
AF321
quantity :
100 ug (also 25 ug)
price :
499 USD
clonality :
polyclonal
host :
domestic goat
conjugate :
nonconjugated
reactivity :
human, bovine
application :
western blot, immunohistochemistry, immunocytochemistry, neutralization, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, proximity ligation assay, blocking or activating experiments
more info or order :
R&D Systems product webpage
citations: 35
Published Application/Species/Sample/DilutionReference
  • western blot; human; fig 5
Fearnley G, Smith G, Abdul Zani I, Yuldasheva N, Mughal N, Homer Vanniasinkam S, et al. VEGF-A isoforms program differential VEGFR2 signal transduction, trafficking and proteolysis. Biol Open. 2016;5:571-83 pubmed publisher
  • blocking or activating experiments; human; 2 ug/ml; fig 7
Wood L, Cox N, Phelps C, Lai S, Poddar A, Talbot C, et al. Thyroid Transcription Factor 1 Reprograms Angiogenic Activities of Secretome. Sci Rep. 2016;6:19857 pubmed publisher
Yong Q, Dijkstra K, van der Keur C, Bruijn J, Eikmans M, Baelde H. MIF Increases sFLT1 Expression in Early Uncomplicated Pregnancy and Preeclampsia. Int J Mol Sci. 2023;24: pubmed publisher
Huang X, Jia L, Jia Y, Xu X, Wang R, Wei M, et al. sFlt-1-enriched exosomes induced endothelial cell dysfunction and a preeclampsia-like phenotype in mice. Cytokine. 2023;166:156190 pubmed publisher
Liu X, Li Z, Sun J, Zhang Z, Li W. Interaction between PD-L1 and soluble VEGFR1 in glioblastoma-educated macrophages. BMC Cancer. 2023;23:259 pubmed publisher
Zakeri F, Latifi Navid H, Soheili Z, Sadeghi M, Arab S, Samiei S, et al. Design, construction and in vivo functional assessment of a hinge truncated sFLT01. Gene Ther. 2022;: pubmed publisher
Wrona A, Aleksandrovych V, Bereza T, Basta P, Gil A, Ulatowska Bia x142 as M, et al. Oviductal Oxygen Homeostasis in Patients with Uterine Myoma: Correlation between Hypoxia and Telocytes. Int J Mol Sci. 2022;23: pubmed publisher
Mitroi D, Tian M, Kawaguchi R, Lowry W, Carmichael S. Single-nucleus transcriptome analysis reveals disease- and regeneration-associated endothelial cells in white matter vascular dementia. J Cell Mol Med. 2022;26:3183-3195 pubmed publisher
Chen J, Wang Y, Wang S, Zhao X, Zhao L, Wang Y. Salvianolic acid B and ferulic acid synergistically promote angiogenesis in HUVECs and zebrafish via regulating VEGF signaling. J Ethnopharmacol. 2022;283:114667 pubmed publisher
Aleksandrovych V, Wrona A, Bereza T, Pitynski K, Gil K. Oviductal Telocytes in Patients with Uterine Myoma. Biomedicines. 2021;9: pubmed publisher
Cheng S, Luo Y, Malachi A, Ko J, Su Q, Xie J, et al. Low-Dose Recombinant Adeno-Associated Virus-Mediated Inhibition of Vascular Endothelial Growth Factor Can Treat Neovascular Pathologies Without Inducing Retinal Vasculitis. Hum Gene Ther. 2021;32:649-666 pubmed publisher
Alqudah A, Eastwood K, Jerotic D, Todd N, Hoch D, McNally R, et al. FKBPL and SIRT-1 Are Downregulated by Diabetes in Pregnancy Impacting on Angiogenesis and Endothelial Function. Front Endocrinol (Lausanne). 2021;12:650328 pubmed publisher
Ichimura S, Ohara K, Kono M, Mizutani K, Kitamura Y, Saga I, et al. Molecular investigation of brain tumors progressing during pregnancy or postpartum period: the association between tumor type, their receptors, and the timing of presentation. Clin Neurol Neurosurg. 2021;207:106720 pubmed publisher
Rudkouskaya A, Smith J, Intes X, Barroso M. Quantification of Trastuzumab-HER2 Engagement In Vitro and In Vivo. Molecules. 2020;25: pubmed publisher
Dong D, Fu Y, Chen F, Zhang J, Jia H, Li J, et al. Hyperoxia sensitizes hypoxic HeLa cells to ionizing radiation by downregulating HIF‑1α and VEGF expression. Mol Med Rep. 2021;23: pubmed publisher
Wei S, Huang Y, Hsieh M, Lin Y, Shyu W, Chen H, et al. Atypical chemokine receptor ACKR3/CXCR7 controls postnatal vasculogenesis and arterial specification by mesenchymal stem cells via Notch signaling. Cell Death Dis. 2020;11:307 pubmed publisher
Tamura R, Tanaka T, Morimoto Y, Kuranari Y, Yamamoto Y, Takei J, et al. Alterations of the tumor microenvironment in glioblastoma following radiation and temozolomide with or without bevacizumab. Ann Transl Med. 2020;8:297 pubmed publisher
Viallard C, Audiger C, Popovic N, Akla N, Lanthier K, Legault Navarrete I, et al. BMP9 signaling promotes the normalization of tumor blood vessels. Oncogene. 2020;39:2996-3014 pubmed publisher
Zsengellér Z, Lo A, Tavasoli M, Pernicone E, Karumanchi S, Rosen S. Soluble fms-Like Tyrosine Kinase 1 Localization in Renal Biopsies of CKD. Kidney Int Rep. 2019;4:1735-1741 pubmed publisher
Yonekura Collier A, Zsengeller Z, Pernicone E, Salahuddin S, Khankin E, Karumanchi S. Placental sFLT1 is associated with complement activation and syncytiotrophoblast damage in preeclampsia. Hypertens Pregnancy. 2019;38:193-199 pubmed publisher
Cao J, Yang R, Smith T, Evans S, McCollum G, Pomerantz S, et al. Human Umbilical Tissue-Derived Cells Secrete Soluble VEGFR1 and Inhibit Choroidal Neovascularization. Mol Ther Methods Clin Dev. 2019;14:37-46 pubmed publisher
Han K, Chang J, Azar D. MMP14-Containing Exosomes Cleave VEGFR1 and Promote VEGFA-Induced Migration and Proliferation of Vascular Endothelial Cells. Invest Ophthalmol Vis Sci. 2019;60:2321-2329 pubmed publisher
Sato M, Tamura R, Tamura H, Mase T, Kosugi K, Morimoto Y, et al. Analysis of Tumor Angiogenesis and Immune Microenvironment in Non-Functional Pituitary Endocrine Tumors. J Clin Med. 2019;8: pubmed publisher
Schaaf M, Houbaert D, Mece O, To S, Ganne M, Maes H, et al. Lysosomal Pathways and Autophagy Distinctively Control Endothelial Cell Behavior to Affect Tumor Vasculature. Front Oncol. 2019;9:171 pubmed publisher
Blanchard T, Czinn S, Banerjee V, Sharda N, Bafford A, Mubariz F, et al. Identification of Cross Talk between FoxM1 and RASSF1A as a Therapeutic Target of Colon Cancer. Cancers (Basel). 2019;11: pubmed publisher
Lee S, Kim H, Shin O, Choi J, Kim J, Cho Y, et al. Intravitreal Injection of AAV Expressing Soluble VEGF Receptor-1 Variant Induces Anti-VEGF Activity and Suppresses Choroidal Neovascularization. Invest Ophthalmol Vis Sci. 2018;59:5398-5407 pubmed publisher
Capossela S, Bertolo A, Gunasekera K, Pötzel T, Baur M, Stoyanov J. VEGF vascularization pathway in human intervertebral disc does not change during the disc degeneration process. BMC Res Notes. 2018;11:333 pubmed publisher
Gonzales S, Badell M, Cottrell H, Rimawi B, Deepak V, Sidell N, et al. Villous explants from preeclamptic placentas induce sFlt1 in PBMCs: An ex vivo co-culture study. Pregnancy Hypertens. 2018;12:40-46 pubmed publisher
Spiel M, Salahuddin S, Pernicone E, Zsengeller Z, Wang A, Modest A, et al. Placental soluble fms-like tyrosine kinase expression in small for gestational age infants and risk for adverse outcomes. Placenta. 2017;52:10-16 pubmed publisher
Basavarajappa H, Sulaiman R, Qi X, Shetty T, Sheik Pran Babu S, Sishtla K, et al. Ferrochelatase is a therapeutic target for ocular neovascularization. EMBO Mol Med. 2017;9:786-801 pubmed publisher
D Alessio A, Proietti G, Lama G, Biamonte F, Lauriola L, Moscato U, et al. Analysis of angiogenesis related factors in glioblastoma, peritumoral tissue and their derived cancer stem cells. Oncotarget. 2016;7:78541-78556 pubmed publisher
Tamura R, Tanaka T, Miyake K, Tabei Y, Ohara K, Sampetrean O, et al. Histopathological investigation of glioblastomas resected under bevacizumab treatment. Oncotarget. 2016;7:52423-52435 pubmed publisher
Chanakira A, Kir D, Barke R, Santilli S, Ramakrishnan S, Roy S. Hypoxia Differentially Regulates Arterial and Venous Smooth Muscle Cell Migration. PLoS ONE. 2015;10:e0138587 pubmed publisher
Kim H, Kim K, Kim B, Cho M, Lee S. The effect of vascular endothelial growth factor on osteoclastogenesis in rheumatoid arthritis. PLoS ONE. 2015;10:e0124909 pubmed publisher
Szalai G, Romero R, Chaiworapongsa T, Xu Y, Wang B, Ahn H, et al. Full-length human placental sFlt-1-e15a isoform induces distinct maternal phenotypes of preeclampsia in mice. PLoS ONE. 2015;10:e0119547 pubmed publisher
product information
brand :
R&D Systems
catalog number base :
AF321
SKU :
AF321
product name :
Human VEGFR1/Flt-1 Antibody
units size :
100 ug (also 25 ug)
description :
The Human VEGFR1/Flt-1 Antibody from R&D Systems is a goat polyclonal antibody to VEGFR1/Flt-1. This antibody reacts with bovine,chinese hamster,human. The Human VEGFR1/Flt-1 Antibody has been validated for the following applications: Blocking,Proximity Ligation Assay (PLA),Flow Cytometry,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Blockade of Receptor-ligand Interaction,Neutralization,Immunocytochemistry,CyTOF-ready,Functional Assay.
target :
VEGFR1/Flt-1
category :
Primary Antibodies
buffer :
Lyophilized from a 0.2 µm filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
clonality :
Polyclonal
concentration :
LYOPH
conjugate :
Unconjugated
all_available conjugate :
Unconjugated
host :
Goat
immunogen :
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human VEGFR1/Flt-1, Ser27-His687, Accession # NP_001153392
isotype :
IgG
purity :
Antigen Affinity-purified
species :
Bovine,Chinese Hamster,Human
specificity :
Detects human VEGFR1/Flt-1 in direct ELISAs and Western blots.
gene symbol :
FLT1
endotoxin note :
<0.10 EU per 1 µg of the antibody by the LAL method.
top caption :
VEGFR1/Flt-1 antibody in Human Breast Cancer Tissue by Immunohistochemistry (IHC-P).
accessionNumbers :
NP_001153392
applications :
Blocking,Proximity Ligation Assay (PLA),Flow Cytometry,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Blockade of Receptor-ligand Interaction,Neutralization,Immunocytochemistry,CyTOF-ready,Functional Assay
USD :
499 USD
alt names :
EC 2.7.10, EC 2.7.10.1, FLT, FLT1, FLT-1, Fms-like tyrosine kinase 1, fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascularpermeability factor receptor), FRT, Tyrosine-protein kinase FRT, Tyrosine-protein kinase receptor FLT, vascular endothelial growth factor receptor 1, Vascular permeability factor receptor, VEGF R1, VEGFR1, VEGFR-1
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
more info or order :
R&D Systems product webpage
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.