product summary
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company name :
R&D Systems
product type :
antibody
product name :
Mouse Nephrin Antibody
catalog :
AF3159
quantity :
100 ug (also 25 ug)
price :
509 USD
clonality :
polyclonal
host :
domestic goat
conjugate :
nonconjugated
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry, immunocytochemistry, neutralization, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
citations: 45
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; mouse; 1:50; loading ...; fig 1a
Kim J, Hwang K, Dang B, Eom M, Kong I, Gwack Y, et al. Insulin-activated store-operated Ca2+ entry via Orai1 induces podocyte actin remodeling and causes proteinuria. Nat Commun. 2021;12:6537 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:10,000; loading ...; fig s5a
Mehta N, Li R, Zhang D, Soomro A, He J, Zhang I, et al. miR299a-5p promotes renal fibrosis by suppressing the antifibrotic actions of follistatin. Sci Rep. 2021;11:88 pubmed publisher
D xf8 rflinger G, Holt C, Thiel S, Bech J, xd8 stergaard J, Bjerre M. Mannan-Binding Lectin Is Associated with Inflammation and Kidney Damage in a Mouse Model of Type 2 Diabetes. Int J Mol Sci. 2024;25: pubmed publisher
Niasse A, Louis K, Lenoir O, Schwarz C, Xu X, Couturier A, et al. Protective Role of the Podocyte IL-15 / STAT5 Pathway in Focal Segmental Glomerulosclerosis. Kidney Int Rep. 2024;9:1093-1106 pubmed publisher
Zhong D, Chen J, Qiao R, Song C, Hao C, Zou Y, et al. Genetic or pharmacologic blockade of mPGES-2 attenuates renal lipotoxicity and diabetic kidney disease by targeting Rev-Erbα/FABP5 signaling. Cell Rep. 2024;43:114075 pubmed publisher
Albrecht M, Sticht C, Wagner T, Hettler S, de la Torre C, Qiu J, et al. The crosstalk between glomerular endothelial cells and podocytes controls their responses to metabolic stimuli in diabetic nephropathy. Sci Rep. 2023;13:17985 pubmed publisher
Tyshynsky R, Sensarma S, Riedl M, Bukowy J, Schramm L, Vulchanova L, et al. Periglomerular afferent innervation of the mouse renal cortex. Front Neurosci. 2023;17:974197 pubmed publisher
Kumar S, Fan X, Rasouly H, Sharma R, Salant D, Lu W. ZEB2 controls kidney stromal progenitor differentiation and inhibits abnormal myofibroblast expansion and kidney fibrosis. JCI Insight. 2023;8: pubmed publisher
Kocylowski M, Aypek H, Bildl W, Helmst xe4 dter M, Trachte P, Dumoulin B, et al. A slit-diaphragm-associated protein network for dynamic control of renal filtration. Nat Commun. 2022;13:6446 pubmed publisher
Jiang S, Alisafaei F, Huang Y, Hong Y, Peng X, Qu C, et al. An ex vivo culture model of kidney podocyte injury reveals mechanosensitive, synaptopodin-templating, sarcomere-like structures. Sci Adv. 2022;8:eabn6027 pubmed publisher
Li L, Feng Y, Zhang J, Zhang Q, Ren J, Sun C, et al. Microtubule associated protein 4 phosphorylation-induced epithelial-to-mesenchymal transition of podocyte leads to proteinuria in diabetic nephropathy. Cell Commun Signal. 2022;20:115 pubmed publisher
Gupta A, Singh K, Fatima S, Ambreen S, Zimmermann S, Younis R, et al. Neutrophil Extracellular Traps Promote NLRP3 Inflammasome Activation and Glomerular Endothelial Dysfunction in Diabetic Kidney Disease. Nutrients. 2022;14: pubmed publisher
Abbad L, Prakoura N, Michon A, Chalghoumi R, Reichelt Wurm S, Banas M, et al. Role of Periostin and Nuclear Factor-κB Interplay in the Development of Diabetic Nephropathy. Cells. 2022;11: pubmed publisher
Bejoy J, Farry J, Peek J, Cabatu M, Williams F, Welch R, et al. Podocytes derived from human induced pluripotent stem cells: characterization, comparison, and modeling of diabetic kidney disease. Stem Cell Res Ther. 2022;13:355 pubmed publisher
Chen X, Wu D, Wu H, Li H, Yang C, Su H, et al. Metformin improves renal injury of MRL/lpr lupus-prone mice via the AMPK/STAT3 pathway. Lupus Sci Med. 2022;9: pubmed publisher
Wang L, Tang Y, Buckley A, Spurney R. Blockade of the natriuretic peptide clearance receptor attenuates proteinuria in a mouse model of focal segmental glomerulosclerosis. Physiol Rep. 2021;9:e15095 pubmed publisher
Ye Q, Zhang Y, Zhuang J, Bi Y, Xu H, Shen Q, et al. The important roles and molecular mechanisms of annexin A2 autoantibody in children with nephrotic syndrome. Ann Transl Med. 2021;9:1452 pubmed publisher
de Ponte M, Cardoso V, Gonçalves G, Costa Pessoa J, Oliveira Souza M. Early type 1 diabetes aggravates renal ischemia/reperfusion-induced acute kidney injury. Sci Rep. 2021;11:19028 pubmed publisher
Sung S. Co-immunostaining of ICAM-1, ICAM-2, and CD31 in Mouse Kidney Glomeruli. Bio Protoc. 2020;10:e3663 pubmed publisher
Wunderlich L, Ströhl F, Ströhl S, Vanderpoorten O, Mascheroni L, Kaminski C. Superresolving the kidney-a practical comparison of fluorescence nanoscopy of the glomerular filtration barrier. Anal Bioanal Chem. 2021;413:1203-1214 pubmed publisher
Ozawa S, Matsubayashi M, Nanaura H, Yanagita M, Mori K, Asanuma K, et al. Proteolytic cleavage of Podocin by Matriptase exacerbates podocyte injury. J Biol Chem. 2020;295:16002-16012 pubmed publisher
Madhusudhan T, Ghosh S, Wang H, Dong W, Gupta D, ElWakiel A, et al. Podocyte Integrin-β 3 and Activated Protein C Coordinately Restrict RhoA Signaling and Ameliorate Diabetic Nephropathy. J Am Soc Nephrol. 2020;31:1762-1780 pubmed publisher
Ganugula R, Arora M, Zou D, Agarwal S, Mohan C, Kumar M. A highly potent lymphatic system-targeting nanoparticle cyclosporine prevents glomerulonephritis in mouse model of lupus. Sci Adv. 2020;6:eabb3900 pubmed publisher
Xiao M, Bohnert B, Aypek H, Kretz O, Grahammer F, Aukschun U, et al. Plasminogen deficiency does not prevent sodium retention in a genetic mouse model of experimental nephrotic syndrome. Acta Physiol (Oxf). 2021;231:e13512 pubmed publisher
Torres A, Muñoz K, Nahuelpán Y, R Saez A, Mendoza P, Jara C, et al. Intraglomerular Monocyte/Macrophage Infiltration and Macrophage-Myofibroblast Transition during Diabetic Nephropathy Is Regulated by the A2B Adenosine Receptor. Cells. 2020;9: pubmed publisher
Lan X, Kumar V, Jha A, Aslam R, Wang H, Chen K, et al. EDA2R mediates podocyte injury in high glucose milieu. Biochimie. 2020;174:74-83 pubmed publisher
Kang H, Lim J, Noh K, Park D, Cho H, Susztak K, et al. Effective reconstruction of functional organotypic kidney spheroid for in vitro nephrotoxicity studies. Sci Rep. 2019;9:17610 pubmed publisher
Qin X, Jiang M, Zhao Y, Gong J, Su H, Yuan F, et al. Berberine protects against diabetic kidney disease via promoting PGC-1α-regulated mitochondrial energy homeostasis. Br J Pharmacol. 2020;177:3646-3661 pubmed publisher
Gao X, Rosales A, Karttunen H, Bommana G, Tandoh B, Yi Z, et al. The HIV protease inhibitor darunavir prevents kidney injury via HIV-independent mechanisms. Sci Rep. 2019;9:15857 pubmed publisher
Majumder S, Hadden M, Thieme K, Batchu S, Niveditha D, Chowdhury S, et al. Dysregulated expression but redundant function of the long non-coding RNA HOTAIR in diabetic kidney disease. Diabetologia. 2019;62:2129-2142 pubmed publisher
He M, Wang J, Yin Z, Zhao Y, Hou H, Fan J, et al. MiR-320a induces diabetic nephropathy via inhibiting MafB. Aging (Albany NY). 2019;11:3055-3079 pubmed publisher
Lin C, Hsu Y, Huang Y, Shih Y, Wang C, Chiang W, et al. A KDM6A-KLF10 reinforcing feedback mechanism aggravates diabetic podocyte dysfunction. EMBO Mol Med. 2019;11: pubmed publisher
Alyodawi K, Vermeij W, Omairi S, Kretz O, Hopkinson M, Solagna F, et al. Compression of morbidity in a progeroid mouse model through the attenuation of myostatin/activin signalling. J Cachexia Sarcopenia Muscle. 2019;10:662-686 pubmed publisher
Chang C, Wang P, Huang T, Taniguchi A. Change in Renal Glomerular Collagens and Glomerular Filtration Barrier-Related Proteins in a Dextran Sulfate Sodium-Induced Colitis Mouse Model. Int J Mol Sci. 2019;20: pubmed publisher
Carota I, Kenig Kozlovsky Y, Onay T, Scott R, Thomson B, Souma T, et al. Targeting VE-PTP phosphatase protects the kidney from diabetic injury. J Exp Med. 2019;: pubmed publisher
Kasinath V, Yilmam O, Uehara M, Yonar M, Jiang L, Li X, et al. Urine podoplanin heralds the onset of ischemia-reperfusion injury of the kidney. Am J Physiol Renal Physiol. 2019;316:F957-F965 pubmed publisher
Li F, Mao X, Zhuang Q, Zhao Z, Zhang Z, Wu H. Inhibiting 4E-BP1 re-activation represses podocyte cell cycle re-entry and apoptosis induced by adriamycin. Cell Death Dis. 2019;10:241 pubmed publisher
Goto T, Hara H, Sanbo M, Masaki H, Sato H, Yamaguchi T, et al. Generation of pluripotent stem cell-derived mouse kidneys in Sall1-targeted anephric rats. Nat Commun. 2019;10:451 pubmed publisher
Yang C, Xue J, An N, Huang X, Wu Z, Ye L, et al. Accelerated Glomerular Cell Senescence in Experimental Lupus Nephritis. Med Sci Monit. 2018;24:6882-6891 pubmed publisher
Wong D, Yiu W, Chan K, Li Y, Li B, Lok S, et al. Activated renal tubular Wnt/β-catenin signaling triggers renal inflammation during overload proteinuria. Kidney Int. 2018;93:1367-1383 pubmed publisher
Alarcón S, Garrido W, Cappelli C, Suárez R, Oyarzun C, Quezada C, et al. Deficient Insulin-mediated Upregulation of the Equilibrative Nucleoside Transporter 2 Contributes to Chronically Increased Adenosine in Diabetic Glomerulopathy. Sci Rep. 2017;7:9439 pubmed publisher
Kato Y, Mori K, Kasahara M, Osaki K, Ishii A, Mori K, et al. Natriuretic peptide receptor guanylyl cyclase-A pathway counteracts glomerular injury evoked by aldosterone through p38 mitogen-activated protein kinase inhibition. Sci Rep. 2017;7:46624 pubmed publisher
Sung S, Ge Y, Dai C, Wang H, Fu S, Sharma R, et al. Dependence of Glomerulonephritis Induction on Novel Intraglomerular Alternatively Activated Bone Marrow-Derived Macrophages and Mac-1 and PD-L1 in Lupus-Prone NZM2328 Mice. J Immunol. 2017;198:2589-2601 pubmed publisher
Mohammed Ali Z, Lu C, Marway M, Carlisle R, Ask K, Lukic D, et al. Endoplasmic reticulum stress inhibition attenuates hypertensive chronic kidney disease through reduction in proteinuria. Sci Rep. 2017;7:41572 pubmed publisher
Fujino T, Hasebe N. Alteration of histone H3K4 methylation in glomerular podocytes associated with proteinuria in patients with membranous nephropathy. BMC Nephrol. 2016;17:179 pubmed
product information
master code :
AF3159
SKU :
AF3159
product name :
Mouse Nephrin Antibody
unit size :
100 ug (also 25 ug)
description :
The Mouse Nephrin Antibody from R&D Systems is a goat polyclonal antibody to Nephrin. This antibody reacts with human,mouse,rat,transgenic mouse. The Mouse Nephrin Antibody has been validated for the following applications: Immunohistochemistry,Flow Cytometry,Western Blot,Neutralization,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunocytochemistry.
target :
Nephrin
category :
Primary Antibodies
buffer :
Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.
clonality :
Polyclonal
concentration :
LYOPH
conjugate :
Unconjugated
host :
Goat
immunogen :
Mouse myeloma cell line NS0-derived recombinant mouse Nephrin, Gln37-Thr1049, Accession # NP_062332
isotype :
IgG
purity :
Antigen Affinity-purified
species :
Human,Mouse,Rat,Transgenic Mouse
specificity :
Detects mouse Nephrin in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human Nephrin is observed.
gene symbol :
NPHS1
top caption :
Nephrin antibody in Mouse Kidney by Immunohistochemistry (IHC-Fr).
accessionNumbers :
NP_062332
applications :
Immunohistochemistry,Flow Cytometry,Western Blot,Neutralization,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunocytochemistry
USD :
509 USD
alt names :
CNF, nephrin, nephrosis 1, congenital, Finnish type (nephrin), NPHNCNF, NPHS1, Renal glomerulus-specific cell adhesion receptor
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.
more info or order :
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.