product summary
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company name :
R&D Systems
product type :
antibody
product name :
Human Tie-2 Antibody
catalog :
AF313
quantity :
100 ug (also 25 ug)
price :
509 USD
clonality :
polyclonal
host :
domestic goat
conjugate :
nonconjugated
reactivity :
human, mouse
application :
western blot, immunohistochemistry, immunocytochemistry, neutralization, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
R&D Systems product webpage
citations: 22
Published Application/Species/Sample/DilutionReference
  • western blot; human; loading ...; fig 6c, s5
Hsieh L, Dos Santos S, Hall B, Ogbechi J, Loglo A, Salguero F, et al. Aberrant stromal tissue factor localisation and mycolactone-driven vascular dysfunction, exacerbated by IL-1β, are linked to fibrin formation in Buruli ulcer lesions. PLoS Pathog. 2022;18:e1010280 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:100; loading ...; fig e10g
Ramachandran P, Dobie R, Wilson Kanamori J, Dora E, Henderson B, Luu N, et al. Resolving the fibrotic niche of human liver cirrhosis at single-cell level. Nature. 2019;575:512-518 pubmed publisher
  • immunoprecipitation; human; loading ...; fig 2d
  • western blot; human; loading ...; fig 2d
Leppänen V, Saharinen P, Alitalo K. Structural basis of Tie2 activation and Tie2/Tie1 heterodimerization. Proc Natl Acad Sci U S A. 2017;114:4376-4381 pubmed publisher
Banerjee K, Lin Y, Gahn J, Cordero J, Gupta P, Mohamed I, et al. SMAD4 maintains the fluid shear stress set point to protect against arterial-venous malformations. J Clin Invest. 2023;133: pubmed publisher
Sato Nishiuchi R, Doiguchi M, Morooka N, Sekiguchi K. Polydom/SVEP1 binds to Tie1 and promotes migration of lymphatic endothelial cells. J Cell Biol. 2023;222: pubmed publisher
Jeon S, Han A, Lee S, Lee S, Lee M, Park S, et al. CD34dim cells identified as pluripotent stem cell-derived definitive hemogenic endothelium purified using bone morphogenetic protein 4. Cell Prolif. 2023;56:e13366 pubmed publisher
Jeon S, Han A, Choi Y, Lee A, Lee J. Lymphoid Lineage γδ T Cells Were Successfully Generated from Human Pluripotent Stem Cells via Hemogenic Endothelium. Int J Stem Cells. 2023;16:108-116 pubmed publisher
Reijrink M, Van Ark J, Lexis C, Visser L, Lodewijk M, van der Horst I, et al. Increased frequency of proangiogenic tunica intima endothelial kinase 2 (Tie2) expressing monocytes in individuals with type 2 diabetes mellitus. Cardiovasc Diabetol. 2022;21:72 pubmed publisher
Jo G, Bae J, Hong H, Han A, Kim D, Hong S, et al. Structural insights into the clustering and activation of Tie2 receptor mediated by Tie2 agonistic antibody. Nat Commun. 2021;12:6287 pubmed publisher
Adjuto Saccone M, Soubeyran P, Garcia J, Audebert S, Camoin L, Rubis M, et al. TNF-α induces endothelial-mesenchymal transition promoting stromal development of pancreatic adenocarcinoma. Cell Death Dis. 2021;12:649 pubmed publisher
Villa E, Critelli R, Lasagni S, Melegari A, Curatolo A, Celsa C, et al. Dynamic angiopoietin-2 assessment predicts survival and chronic course in hospitalized patients with COVID-19. Blood Adv. 2021;5:662-673 pubmed publisher
Heil F, Babitzki G, Julien Laferrière A, Ooi C, Hidalgo M, Massard C, et al. Vanucizumab mode of action: Serial biomarkers in plasma, tumor, and skin-wound-healing biopsies. Transl Oncol. 2021;14:100984 pubmed publisher
Griffin M, Sorum A, Miller G, Goddard W, Hsieh Wilson L. Sulfated glycans engage the Ang-Tie pathway to regulate vascular development. Nat Chem Biol. 2021;17:178-186 pubmed publisher
Han Q, Zhang Q, Ying F, Wang Z, Zhang Y, Gong L, et al. Circulating Tie2-Expressing Monocytes: A Potential Biomarker for Cervical Cancer. Onco Targets Ther. 2020;13:8877-8885 pubmed publisher
Chatterjee A, Eshwaran R, Huang H, Zhao D, Schmidt M, Wieland T, et al. Role of the Ang2-Tie2 Axis in Vascular Damage Driven by High Glucose or Nucleoside Diphosphate Kinase B Deficiency. Int J Mol Sci. 2020;21: pubmed publisher
Gu J, Zhang Y, Han Z, Gao L, Cui J, Sun Y, et al. Targeting the ERβ/Angiopoietin-2/Tie-2 signaling-mediated angiogenesis with the FDA-approved anti-estrogen Faslodex to increase the Sunitinib sensitivity in RCC. Cell Death Dis. 2020;11:367 pubmed publisher
Cossutta M, Darche M, Carpentier G, Houppe C, Ponzo M, Raineri F, et al. Weibel-Palade Bodies Orchestrate Pericytes During Angiogenesis. Arterioscler Thromb Vasc Biol. 2019;39:1843-1858 pubmed publisher
Besnier M, Shantikumar S, Anwar M, Dixit P, Chamorro Jorganes A, Sweaad W, et al. miR-15a/-16 Inhibit Angiogenesis by Targeting the Tie2 Coding Sequence: Therapeutic Potential of a miR-15a/16 Decoy System in Limb Ischemia. Mol Ther Nucleic Acids. 2019;17:49-62 pubmed publisher
Kim J, Park J, Choi J, Park I, Hwang Y, Bae H, et al. Tie2 activation promotes choriocapillary regeneration for alleviating neovascular age-related macular degeneration. Sci Adv. 2019;5:eaau6732 pubmed publisher
Tsujimoto T, Sudo H, Todoh M, Yamada K, Iwasaki K, Ohnishi T, et al. An acellular bioresorbable ultra-purified alginate gel promotes intervertebral disc repair: A preclinical proof-of-concept study. EBioMedicine. 2018;37:521-534 pubmed publisher
Sampedro Núñez M, Luque R, Ramos Leví A, Gahete M, Serrano Somavilla A, Villa Osaba A, et al. Presence of sst5TMD4, a truncated splice variant of the somatostatin receptor subtype 5, is associated to features of increased aggressiveness in pancreatic neuroendocrine tumors. Oncotarget. 2016;7:6593-608 pubmed publisher
Hakanpaa L, Sipilä T, Leppänen V, Gautam P, Nurmi H, Jacquemet G, et al. Endothelial destabilization by angiopoietin-2 via integrin β1 activation. Nat Commun. 2015;6:5962 pubmed publisher
product information
master code :
AF313
SKU :
AF313
product name :
Human Tie-2 Antibody
unit size :
100 ug (also 25 ug)
description :
The Human Tie-2 Antibody from R&D Systems is a goat polyclonal antibody to Tie-2. This antibody reacts with human,mouse,primate - papio anubis (olive baboon),rabbit,rat. The Human Tie-2 Antibody has been validated for the following applications: Western Blot,Flow Cytometry,Immunocytochemistry,Neutralization,Blockade of Receptor-ligand Interaction,Immunoprecipitation,Immunohistochemistry,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Simple Western.
target :
Tie-2
category :
Primary Antibodies
buffer :
Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.
clonality :
Polyclonal
concentration :
LYOPH
conjugate :
Unconjugated
host :
Goat
immunogen :
Mouse myeloma cell line NS0-derived recombinant human Tie-2, Ala23-Lys745, Accession # Q02763
isotype :
IgG
purity :
Antigen Affinity-purified
species :
Human,Mouse,Primate - Papio anubis (Olive Baboon),Rabbit
specificity :
Detects human Tie-2 in direct ELISAs and Western blots.
gene symbol :
TEK
endotoxin note :
<0.10 EU per 1 ╡g of the antibody by the LAL method.
top caption :
Detection of Human Tie-2 antibody by Western Blot.
accessionNumbers :
Q02763
applications :
Western Blot,Neutralization,Blockade of Receptor-ligand Interaction,Flow Cytometry,Immunoprecipitation,Immunohistochemistry,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunocytochemistry,Simple Western
USD :
509 USD
alt names :
angiopoietin-1 receptor, CD202B, CD202b antigen, EC 2.7.10, EC 2.7.10.1, hTIE2, p140 TEK, soluble TIE2 variant 1, soluble TIE2 variant 2, TEK, TEK tyrosine kinase, endothelial, Tie2, TIE-2, TIE2CD202b, Tunica interna endothelial cell kinase, Tyrosine-protein kinase receptor TEK, Tyrosine-protein kinase receptor TIE-2, venous malformations, multiple cutaneous and mucosal, VMCM, VMCM1
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.
more info or order :
R&D Systems product webpage
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.