Human EphA2 Antibody | R&D Systems AF3035 product information

product summary

company name :

R&D Systems

product type :

antibody

product name :

Human EphA2 Antibody

catalog :

AF3035

quantity :

100 ug (also 25 ug)

price :

509 USD

clonality :

polyclonal

host :

domestic goat

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

R&D Systems product webpage

citations: 9

Reference
Torlot L, Jarzab A, Albert J, P xf3 k Udvari x, Stahler A, Holch J, et al. Proteomics uncover EPHA2 as a potential novel therapeutic target in colorectal cancer cell lines with acquired cetuximab resistance. J Cancer Res Clin Oncol. 2023;149:669-682 pubmed publisher
Trivieri N, Visioli A, Mencarelli G, Cariglia M, Marongiu L, Pracella R, et al. Growth factor independence underpins a paroxysmal, aggressive Wnt5aHigh/EphA2Low phenotype in glioblastoma stem cells, conducive to experimental combinatorial therapy. J Exp Clin Cancer Res. 2022;41:139 pubmed publisher
Jukonen J, Moyano Galceran L, Höpfner K, Pietilä E, Lehtinen L, Huhtinen K, et al. Aggressive and recurrent ovarian cancers upregulate ephrinA5, a non-canonical effector of EphA2 signaling duality. Sci Rep. 2021;11:8856 pubmed publisher
Prinzing B, Schreiner P, Bell M, Fan Y, Krenciute G, Gottschalk S. MyD88/CD40 signaling retains CAR T cells in a less differentiated state. JCI Insight. 2020;5: pubmed publisher
Moyano Galceran L, Pietilä E, Turunen S, Corvigno S, Hjerpe E, Bulanova D, et al. Adaptive RSK-EphA2-GPRC5A signaling switch triggers chemotherapy resistance in ovarian cancer. EMBO Mol Med. 2020;12:e11177 pubmed publisher
Visioli A, Giani F, Trivieri N, Pracella R, Miccinilli E, Cariglia M, et al. Stemness underpinning all steps of human colorectal cancer defines the core of effective therapeutic strategies. EBioMedicine. 2019;: pubmed publisher
Xu M, Wei P, Suo M, Hu Y, Ding W, Su L, et al. Brucine Suppresses Vasculogenic Mimicry in Human Triple-Negative Breast Cancer Cell Line MDA-MB-231. Biomed Res Int. 2019;2019:6543230 pubmed publisher
Reátegui E, van der Vos K, Lai C, Zeinali M, Atai N, Aldikacti B, et al. Engineered nanointerfaces for microfluidic isolation and molecular profiling of tumor-specific extracellular vesicles. Nat Commun. 2018;9:175 pubmed publisher
Barquilla A, Lamberto I, Noberini R, Heynen Genel S, Brill L, Pasquale E. Protein kinase A can block EphA2 receptor-mediated cell repulsion by increasing EphA2 S897 phosphorylation. Mol Biol Cell. 2016;27:2757-70 pubmed publisher

product information

brand :

R&D Systems

master code :

AF3035

SKU :

AF3035

product name :

Human EphA2 Antibody

unit size :

100 ug (also 25 ug)

seo description :

The Human EphA2 Antibody from R&D Systems is a goat polyclonal antibody to EphA2. This antibody reacts with human,primate. The Human EphA2 Antibody has been validated for the following applications: Immunohistochemistry,Flow Cytometry,Western Blot,Functional Assay,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunocytochemistry,CyTOF-ready,Knockout Validated.

target :

EphA2

category :

Primary Antibodies

buffer :

Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.

clonality :

Polyclonal

concentration :

LYOPH

conjugate :

Unconjugated

dilution :

Western Blot 1 ug/mL, Flow Cytometry 0.25 ug/10^6 cells, Immunohistochemistry 5-15 ug/mL, CyTOF-ready, Knockout Validated 1 ug/mL

host :

Goat

immunogen :

Mouse myeloma cell line NS0-derived recombinant human EphA2, Gln25-Asn534, Accession # P29317

isotype :

IgG

purity :

Antigen Affinity-purified

species :

Human,Primate

specificity :

Detects human EphA2 in direct ELISAs and Western blots. In direct ELISAs, less than 45% cross-reactivity with recombinant mouse EphA2 is observed and less than 1% cross-reactivity with recombinant human (rh) EphA1, rhEphA3, rhEphA4, rhEphA5, rhEphA6, rhEphA7 and rhEphA10 is observed.

gene symbol :

EPHA2

top caption :

Detection of Human EphA2 antibody by Western Blot.

accessionNumbers :

P29317

applications :

CyTOF-ready,Knockout Validated,Flow Cytometry,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunocytochemistry,Functional Assay

USD :

509 USD

alt names :

ARCC2, EC 2.7.10, EC 2.7.10.1, Eck, ECKepithelial cell receptor protein tyrosine kinase, EPH receptor A2, EphA2, ephrin type-A receptor 2, Epithelial cell kinase, Myk2, Sek2, soluble EPHA2 variant 1, Tyrosine-protein kinase receptor ECK

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage