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company name :
R&D Systems
product type :
antibody
product name :
Rat Nestin Antibody
catalog :
AF2736
quantity :
100 ug (also 25 ug)
price :
509 USD
clonality :
polyclonal
host :
domestic goat
conjugate :
nonconjugated
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
citations: 12
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; mouse; 1:500; loading ...; fig 1g
  • western blot; mouse; 1:2000; loading ...; fig s1h
O Shea T, Ao Y, Wang S, Wollenberg A, Kim J, Ramos Espinoza R, et al. Lesion environments direct transplanted neural progenitors towards a wound repair astroglial phenotype in mice. Nat Commun. 2022;13:5702 pubmed publisher
  • immunohistochemistry - paraffin section; rat; 1:20; loading ...; fig 1a
Gustorff C, Scheuer T, Schmitz T, Buhrer C, Endesfelder S. GABAB Receptor-Mediated Impairment of Intermediate Progenitor Maturation During Postnatal Hippocampal Neurogenesis of Newborn Rats. Front Cell Neurosci. 2021;15:651072 pubmed publisher
Jim xe9 nez Madrona E, Morado D xed az C, Talaver xf3 n R, Tabernero A, Pastor A, S xe1 ez J, et al. Antiproliferative effect of boldine on neural progenitor cells and on glioblastoma cells. Front Neurosci. 2023;17:1211467 pubmed publisher
Fong B, Chakroun I, Iqbal M, Paul S, Bastasic J, O Neil D, et al. The Rb/E2F axis is a key regulator of the molecular signatures instructing the quiescent and activated adult neural stem cell state. Cell Rep. 2022;41:111578 pubmed publisher
Miloudi S, Valensi M, El Sanharawi M, Abitbol M, Behar Cohen F, Versaux Botteri C. Nestin contributes to laser choroidal and retinal neovascularization. Mol Vis. 2022;28:280-299 pubmed
Lithopoulos M, Toussay X, Zhong S, Xu L, Mustafa S, Ouellette J, et al. Neonatal hyperoxia in mice triggers long-term cognitive deficits via impairments in cerebrovascular function and neurogenesis. J Clin Invest. 2022;132: pubmed publisher
Chen L, Zhang M, Fang L, Yang X, Cao N, Xu L, et al. Coordinated regulation of the ribosome and proteasome by PRMT1 in the maintenance of neural stemness in cancer cells and neural stem cells. J Biol Chem. 2021;297:101275 pubmed publisher
Lavado A, Gangwar R, Par xe9 J, Wan S, Fan Y, Cao X. YAP/TAZ maintain the proliferative capacity and structural organization of radial glial cells during brain development. Dev Biol. 2021;480:39-49 pubmed publisher
Sugiura A, Shimizu T, Kameyama T, Maruo T, Kedashiro S, Miyata M, et al. Identification of Sox2 and NeuN Double-Positive Cells in the Mouse Hypothalamic Arcuate Nucleus and Their Reduction in Number With Aging. Front Aging Neurosci. 2020;12:609911 pubmed publisher
Xi K, Gu Y, Tang J, Chen H, Xu Y, Wu L, et al. Microenvironment-responsive immunoregulatory electrospun fibers for promoting nerve function recovery. Nat Commun. 2020;11:4504 pubmed publisher
Cheng S, Liu T, Hu Y, Xia Y, Hou J, Huang C, et al. Conditional Inactivation of Pen-2 in the Developing Neocortex Leads to Rapid Switch of Apical Progenitors to Basal Progenitors. J Neurosci. 2019;39:2195-2207 pubmed publisher
Takamori Y, Hirahara Y, Wakabayashi T, Mori T, Koike T, Kataoka Y, et al. Differential expression of nuclear lamin subtypes in the neural cells of the adult rat cerebral cortex. IBRO Rep. 2018;5:99-109 pubmed publisher
product information
brand :
R&D Systems
master code :
AF2736
SKU :
AF2736
product name :
Rat Nestin Antibody
unit size :
100 ug (also 25 ug)
seo description :
The Rat Nestin Antibody from R&D Systems is a goat polyclonal antibody to Nestin. This antibody reacts with human,mouse,rat. The Rat Nestin Antibody has been validated for the following applications: Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunofluorescence,Immunocytochemistry.
target :
Nestin
category :
Primary Antibodies
buffer :
Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.
clonality :
Polyclonal
conjugate :
Unconjugated
dilution :
Western Blot 0.1 ug/mL, Immunohistochemistry 5-15 ug/mL, Immunocytochemistry 5-15 ug/mL
host :
Goat
immunogen :
E. coli -derived recombinant rat Nestin, Met544-Glu820 (Gly756Asp, Ile758Met, Arg572Lys, Ala574Pro, Ile802Met, Arg816Lys), Accession # EDM00749
isotype :
IgG
purity :
Antigen Affinity-purified
species :
Human,Mouse,Rat
specificity :
Detects rat Nestin in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse Nestin is observed.
gene symbol :
NES
top caption :
Nestin antibody in Rat Cortical Stem Cells by Immunocytochemistry (ICC).
accessionNumbers :
EDM00749
applications :
Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunofluorescence,Immunocytochemistry
USD :
509 USD
alt names :
FLJ21841, NES, nestin
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.
more info or order :
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.