Human/Mouse Phospho-Tie-2 (Y992) Antibody | R&D Systems AF2720 product information

product summary

company name :

R&D Systems

product type :

antibody

product name :

Human/Mouse Phospho-Tie-2 (Y992) Antibody

catalog :

AF2720

quantity :

50 ug (also 25 ug)

price :

399 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, FACS, immunohistochemistry - frozen section, immunohistochemistry - free floating section

more info or order :

R&D Systems product webpage

citations: 24

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - frozen section; mouse; 1:100; loading ...; fig 5e	Paredes I, Vieira J, Shah B, Ramunno C, Dyckow J, Adler H, et al. Oligodendrocyte precursor cell specification is regulated by bidirectional neural progenitor-endothelial cell crosstalk. Nat Neurosci. 2021;24:478-488 pubmed publisher
western blot; human; loading ...; fig s2a	Juettner V, Kruse K, Dan A, Vu V, Khan Y, Le J, et al. VE-PTP stabilizes VE-cadherin junctions and the endothelial barrier via a phosphatase-independent mechanism. J Cell Biol. 2019;218:1725-1742 pubmed publisher
immunohistochemistry - frozen section; mouse; loading ...; fig 1a	Kim J, Park D, Bae H, Park D, Kim D, Lee C, et al. Impaired angiopoietin/Tie2 signaling compromises Schlemm's canal integrity and induces glaucoma. J Clin Invest. 2017;127:3877-3896 pubmed publisher
immunocytochemistry; human; loading ...; fig 2c western blot; human; loading ...; fig 2d	LeppÃ¤nen V, Saharinen P, Alitalo K. Structural basis of Tie2 activation and Tie2/Tie1 heterodimerization. Proc Natl Acad Sci U S A. 2017;114:4376-4381 pubmed publisher
immunohistochemistry - frozen section; human; 1:100; loading ...; fig 2h immunohistochemistry - frozen section; mouse; 1:100; loading ...; fig 5f	Jenny Zhou H, Qin L, Zhang H, Tang W, Ji W, He Y, et al. Endothelial exocytosis of angiopoietin-2 resulting from CCM3 deficiency contributes to cerebral cavernous malformation. Nat Med. 2016;22:1033-1042 pubmed publisher
immunohistochemistry - free floating section; mouse; 1:50; fig 9 western blot; mouse; 1:400; fig 8	Gurnik S, Devraj K, Macas J, Yamaji M, Starke J, Scholz A, et al. Angiopoietin-2-induced blood-brain barrier compromise and increased stroke size are rescued by VE-PTP-dependent restoration of Tie2 signaling. Acta Neuropathol. 2016;131:753-73 pubmed publisher
	Pan Z, Yao Q, Kong W, Ma X, Tian L, Zhao Y, et al. Generation of iPSC-derived human venous endothelial cells for the modeling of vascular malformations and drug discovery. Cell Stem Cell. 2025;32:227-245.e9 pubmed publisher
	Lee E, Lee E, Kong E, Chon H, Llaiqui Condori M, Park C, et al. An agonistic anti-Tie2 antibody suppresses the normal-to-tumor vascular transition in the glioblastoma invasion zone. Exp Mol Med. 2023;55:470-484 pubmed publisher
	Shirakura K, Baluk P, Nottebaum A, Ipe U, Peters K, McDonald D, et al. Shear stress control of vascular leaks and atheromas through Tie2 activation by VE-PTP sequestration. EMBO Mol Med. 2023;15:e16128 pubmed publisher
	Lask A, Gutbier B, Kershaw O, Nouailles G, Gruber A, M xfc ller Redetzky H, et al. Adjunctive therapy with the Tie2 agonist Vasculotide reduces pulmonary permeability in Streptococcus pneumoniae infected and mechanically ventilated mice. Sci Rep. 2022;12:15531 pubmed publisher
	Choe Y, Yoon J, Joo J, Kim B, Hong S, Koh G, et al. Pericyte Loss Leads to Capillary Stalling Through Increased Leukocyte-Endothelial Cell Interaction in the Brain. Front Cell Neurosci. 2022;16:848764 pubmed publisher
	Griffin M, Sorum A, Miller G, Goddard W, Hsieh Wilson L. Sulfated glycans engage the Ang-Tie pathway to regulate vascular development. Nat Chem Biol. 2021;17:178-186 pubmed publisher
	Gu J, Zhang Y, Han Z, Gao L, Cui J, Sun Y, et al. Targeting the ERβ/Angiopoietin-2/Tie-2 signaling-mediated angiogenesis with the FDA-approved anti-estrogen Faslodex to increase the Sunitinib sensitivity in RCC. Cell Death Dis. 2020;11:367 pubmed publisher
	Singhal M, Gengenbacher N, La Porta S, Gehrs S, Shi J, Kamiyama M, et al. Preclinical validation of a novel metastasis-inhibiting Tie1 function-blocking antibody. EMBO Mol Med. 2020;12:e11164 pubmed publisher
	Besnier M, Shantikumar S, Anwar M, Dixit P, Chamorro Jorganes A, Sweaad W, et al. miR-15a/-16 Inhibit Angiogenesis by Targeting the Tie2 Coding Sequence: Therapeutic Potential of a miR-15a/16 Decoy System in Limb Ischemia. Mol Ther Nucleic Acids. 2019;17:49-62 pubmed publisher
	Kato K, Diéguez Hurtado R, Park D, Hong S, Kato Azuma S, Adams S, et al. Pulmonary pericytes regulate lung morphogenesis. Nat Commun. 2018;9:2448 pubmed publisher
	Lu Y, Tai P, Ai J, Gessler D, Su Q, Yao X, et al. Transcriptome Profiling of Neovascularized Corneas Reveals miR-204 as a Multi-target Biotherapy Deliverable by rAAVs. Mol Ther Nucleic Acids. 2018;10:349-360 pubmed publisher
	Ko D, Kim S. Cooperation between ZEB2 and Sp1 promotes cancer cell survival and angiogenesis during metastasis through induction of survivin and VEGF. Oncotarget. 2018;9:726-742 pubmed publisher
	Higgins S, De Ceunynck K, Kellum J, Chen X, Gu X, Chaudhry S, et al. Tie2 protects the vasculature against thrombus formation in systemic inflammation. J Clin Invest. 2018;128:1471-1484 pubmed publisher
	Shah A, Birdsey G, Peghaire C, Pitulescu M, Dufton N, Yang Y, et al. The endothelial transcription factor ERG mediates Angiopoietin-1-dependent control of Notch signalling and vascular stability. Nat Commun. 2017;8:16002 pubmed publisher
	Alawo D, Tahir T, Fischer M, Bates D, Amirova S, Brindle N. Regulation of Angiopoietin Signalling by Soluble Tie2 Ectodomain and Engineered Ligand Trap. Sci Rep. 2017;7:3658 pubmed publisher
	Shlamkovich T, Aharon L, Barton W, Papo N. Utilizing combinatorial engineering to develop Tie2 targeting antagonistic angiopoetin-2 ligands as candidates for anti-angiogenesis therapy. Oncotarget. 2017;8:33571-33585 pubmed publisher
	Bezabeh B, Fleming R, Fazenbaker C, Zhong H, Coffman K, Yu X, et al. Insertion of scFv into the hinge domain of full-length IgG1 monoclonal antibody results in tetravalent bispecific molecule with robust properties. MAbs. 2017;9:240-256 pubmed publisher
	Shah P, Lueschen N, Ardestani A, Oberholzer J, Olerud J, Carlsson P, et al. Angiopoetin-2 Signals Do Not Mediate the Hypervascularization of Islets in Type 2 Diabetes. PLoS ONE. 2016;11:e0161834 pubmed publisher

product information

master code :

AF2720

SKU :

AF2720

product name :

Human/Mouse Phospho-Tie-2 (Y992) Antibody

unit size :

50 ug (also 25 ug)

description :

The Human/Mouse Phospho-Tie-2 (Y992) Antibody from R&D Systems is a rabbit polyclonal antibody to Tie-2. This antibody reacts with human,mouse,rat. The Human/Mouse Phospho-Tie-2 (Y992) Antibody has been validated for the following applications: Western Blot,FACS,Immunohistochemistry,Immunocytochemistry,Intracellular Staining by Flow Cytometry,CyTOF-ready.

target :

Tie-2

category :

Primary Antibodies

buffer :

Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.

clonality :

Polyclonal

concentration :

LYOPH

conjugate :

Unconjugated

host :

Rabbit

immunogen :

Phosphopeptide containing human Tie-2 Y992 site

isotype :

IgG

purity :

Antigen Affinity-purified

species :

Human,Mouse,Rat

specificity :

Detects human and mouse Tie-2 when phosphorylated at Y992 in Western blots.

gene symbol :

TEK

top caption :

Detection of Mouse Phospho-Tie-2 (Y992) antibody by Western Blot.

applications :

Western Blot,FACS,Immunohistochemistry,Immunocytochemistry,Intracellular Staining by Flow Cytometry,CyTOF-ready

USD :

399 USD

alt names :

angiopoietin-1 receptor, CD202B, CD202b antigen, EC 2.7.10, EC 2.7.10.1, hTIE2, p140 TEK, soluble TIE2 variant 1, soluble TIE2 variant 2, TEK, TEK tyrosine kinase, endothelial, Tie2, TIE-2, TIE2CD202b, Tunica interna endothelial cell kinase, Tyrosine-protein kinase receptor TEK, Tyrosine-protein kinase receptor TIE-2, venous malformations, multiple cutaneous and mucosal, VMCM, VMCM1

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage