product summary
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company name :
R&D Systems
product type :
antibody
product name :
Mouse/Rat Complement Component C3d Antibody
catalog :
AF2655
quantity :
100 ug (also 25 ug)
price :
509 USD
clonality :
polyclonal
host :
domestic goat
conjugate :
nonconjugated
reactivity :
human, mouse, rat
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
citations: 38
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - paraffin section; mouse; 1:100; loading ...; fig 5c
Pellegrini P, Hervera A, Varea O, Brewer M, L xf3 pez Soldado I, Guitart A, et al. Lack of p62 Impairs Glycogen Aggregation and Exacerbates Pathology in a Mouse Model of Myoclonic Epilepsy of Lafora. Mol Neurobiol. 2022;59:1214-1229 pubmed publisher
Jiao M, Wang C, Tang X, Dai C, Zhang N, Fan A, et al. Active secretion of IL-33 from astrocytes is dependent on TMED10 and promotes central nervous system homeostasis. Brain Behav Immun. 2024;119:539-553 pubmed publisher
Oizumi H, Miyamoto Y, Seiwa C, Yamamoto M, Yoshioka N, Iizuka S, et al. Lethal adulthood myelin breakdown by oligodendrocyte-specific Ddx54 knockout. iScience. 2023;26:107448 pubmed publisher
Feng S, Wu C, Zou P, Deng Q, Chen Z, Li M, et al. High-intensity interval training ameliorates Alzheimer's disease-like pathology by regulating astrocyte phenotype-associated AQP4 polarization. Theranostics. 2023;13:3434-3450 pubmed publisher
He J, Li D, Wen Q, Qin T, Long H, Zhang S, et al. Synergistic effects of lipopolysaccharide and rotenone on dopamine neuronal damage in rats. CNS Neurosci Ther. 2023;29:2281-2291 pubmed publisher
Sun X, Ni Y, Lu Q, Liang Y, Gu M, Xue X, et al. Mechanistic target of rapamycin complex 1 orchestrates the interplay between hepatocytes and Kupffer cells to determine the outcome of immune-mediated hepatitis. Cell Death Dis. 2022;13:1031 pubmed publisher
Zhu X, Schrader J, Irizarry B, Smith S, Van Nostrand W. Impact of Aβ40 and Aβ42 Fibrils on the Transcriptome of Primary Astrocytes and Microglia. Biomedicines. 2022;10: pubmed publisher
Kofoed R, Noseworthy K, Wu K, Sivadas S, Stanek L, Elmer B, et al. The engineered AAV2-HBKO promotes non-invasive gene delivery to large brain regions beyond ultrasound targeted sites. Mol Ther Methods Clin Dev. 2022;27:167-184 pubmed publisher
Zhang W, Xiao D, Li X, Zhang Y, Rasouli J, Casella G, et al. SIRT1 inactivation switches reactive astrocytes to an antiinflammatory phenotype in CNS autoimmunity. J Clin Invest. 2022;132: pubmed publisher
Zhang Q, Liu C, Shi R, Zhou S, Shan H, Deng L, et al. Blocking C3d+/GFAP+ A1 Astrocyte Conversion with Semaglutide Attenuates Blood-Brain Barrier Disruption in Mice after Ischemic Stroke. Aging Dis. 2022;13:943-959 pubmed publisher
Van Zeller M, Sebasti xe3 o A, Valente C. Microglia Depletion from Primary Glial Cultures Enables to Accurately Address the Immune Response of Astrocytes. Biomolecules. 2022;12: pubmed publisher
Wan T, Zhu W, Zhao Y, Zhang X, Ye R, Zuo M, et al. Astrocytic phagocytosis contributes to demyelination after focal cortical ischemia in mice. Nat Commun. 2022;13:1134 pubmed publisher
Li H, Tang Z, Wang Z, Lv J, Liu X, Liang Y, et al. C-Reactive Protein Protects Against Acetaminophen-Induced Liver Injury by Preventing Complement Overactivation. Cell Mol Gastroenterol Hepatol. 2022;13:289-307 pubmed publisher
Reuter S, Kentrup D, Grabner A, Kohler G, Buscher K, Edemir B. C4d Deposition after Allogeneic Renal Transplantation in Rats Is Involved in Initial Apoptotic Cell Clearance. Cells. 2021;10: pubmed publisher
Kofoed R, Heinen S, Silburt J, Dubey S, Dibia C, Maes M, et al. Transgene distribution and immune response after ultrasound delivery of rAAV9 and PHP.B to the brain in a mouse model of amyloidosis. Mol Ther Methods Clin Dev. 2021;23:390-405 pubmed publisher
Bongoni A, Vikstrom I, McRae J, Salvaris E, Fisicaro N, Pearse M, et al. A potent truncated form of human soluble CR1 is protective in a mouse model of renal ischemia-reperfusion injury. Sci Rep. 2021;11:21873 pubmed publisher
Shen F, Liu M, Pei F, Yu L, Yang X. Role of uromodulin and complement activation in the progression of kidney disease. Oncol Lett. 2021;22:829 pubmed publisher
Lu Q, Hou Q, Cao K, Sun X, Liang Y, Gu M, et al. Complement factor B in high glucose-induced podocyte injury and diabetic kidney disease. JCI Insight. 2021;6: pubmed publisher
Enzbrenner A, Zulliger R, Biber J, Pousa A, Sch xe4 fer N, Stucki C, et al. Sodium Iodate-Induced Degeneration Results in Local Complement Changes and Inflammatory Processes in Murine Retina. Int J Mol Sci. 2021;22: pubmed publisher
Zhang L, Qian Y, Li J, Zhou X, Xu H, Yan J, et al. BAD-mediated neuronal apoptosis and neuroinflammation contribute to Alzheimer's disease pathology. iScience. 2021;24:102942 pubmed publisher
de Mattos Barbosa M, Lefferts A, Huynh D, Liu H, Zhang Y, Fu B, et al. TNFRSF13B genotypes control immune-mediated pathology by regulating the functions of innate B cells. JCI Insight. 2021;6: pubmed publisher
Qiu W, Luo S, Ma S, Saminathan P, Li H, Gunnersen J, et al. The Sez6 Family Inhibits Complement by Facilitating Factor I Cleavage of C3b and Accelerating the Decay of C3 Convertases. Front Immunol. 2021;12:607641 pubmed publisher
Kim H, Triplet E, Radulovic M, Bouchal S, Kleppe L, Simon W, et al. The thrombin receptor modulates astroglia-neuron trophic coupling and neural repair after spinal cord injury. Glia. 2021;69:2111-2132 pubmed publisher
Hijaze N, Ledersnaider M, Simanovich E, Kassem S, Rahat M. Inducing regulated necrosis and shifting macrophage polarization with anti-EMMPRIN antibody (161-pAb) and complement factors. J Leukoc Biol. 2020;: pubmed publisher
Jabri Y, Biber J, Díaz Lezama N, Grosche A, Pauly D. Cell-Type-Specific Complement Profiling in the ABCA4-/- Mouse Model of Stargardt Disease. Int J Mol Sci. 2020;21: pubmed publisher
Lu Y, Sareddy G, Wang J, Zhang Q, Tang F, Pratap U, et al. Neuron-Derived Estrogen is Critical for Astrocyte Activation and Neuroprotection of the Ischemic Brain. J Neurosci. 2020;: pubmed publisher
Hou B, Zhang Y, Liang P, He Y, Peng B, Liu W, et al. Inhibition of the NLRP3-inflammasome prevents cognitive deficits in experimental autoimmune encephalomyelitis mice via the alteration of astrocyte phenotype. Cell Death Dis. 2020;11:377 pubmed publisher
Alawieh A, Langley E, Feng W, Spiotta A, Tomlinson S. Complement-Dependent Synaptic Uptake and Cognitive Decline after Stroke and Reperfusion Therapy. J Neurosci. 2020;40:4042-4058 pubmed publisher
Hammond J, Bellizzi M, Ware C, Qiu W, Saminathan P, Li H, et al. Complement-dependent synapse loss and microgliosis in a mouse model of multiple sclerosis. Brain Behav Immun. 2020;87:739-750 pubmed publisher
Smith H, Freeman O, Butcher A, Holmqvist S, Humoud I, Schätzl T, et al. Astrocyte Unfolded Protein Response Induces a Specific Reactivity State that Causes Non-Cell-Autonomous Neuronal Degeneration. Neuron. 2020;: pubmed publisher
Yoon H, Choi C, Triplet E, Langley M, Kleppe L, Kim H, et al. Blocking the Thrombin Receptor Promotes Repair of Demyelinated Lesions in the Adult Brain. J Neurosci. 2020;: pubmed publisher
Campbell S, Muñoz Ballester C, Chaunsali L, Mills W, Yang J, Sontheimer H, et al. Potassium and glutamate transport is impaired in scar-forming tumor-associated astrocytes. Neurochem Int. 2020;133:104628 pubmed publisher
Chao C, Gutiérrez Vázquez C, Rothhammer V, Mayo L, Wheeler M, Tjon E, et al. Metabolic Control of Astrocyte Pathogenic Activity via cPLA2-MAVS. Cell. 2019;179:1483-1498.e22 pubmed publisher
Itto R, Oe Y, Imaruoka K, Sato E, Sekimoto A, Yamakage S, et al. Glomerular Injury Is Exacerbated in Lupus-Prone MRL/lpr Mice Treated with a Protease-Activated Receptor 2 Antagonist. Tohoku J Exp Med. 2019;249:127-133 pubmed publisher
Hu C, Li L, Ding P, Li L, Ge X, Zheng L, et al. Complement Inhibitor CRIg/FH Ameliorates Renal Ischemia Reperfusion Injury via Activation of PI3K/AKT Signaling. J Immunol. 2018;201:3717-3730 pubmed publisher
Alawieh A, Andersen M, Adkins D, Tomlinson S. Acute Complement Inhibition Potentiates Neurorehabilitation and Enhances tPA-Mediated Neuroprotection. J Neurosci. 2018;38:6527-6545 pubmed publisher
Alawieh A, Langley E, Weber S, Adkins D, Tomlinson S. Identifying the Role of Complement in Triggering Neuroinflammation after Traumatic Brain Injury. J Neurosci. 2018;38:2519-2532 pubmed publisher
Platt J, Silva I, Balin S, Lefferts A, Farkash E, Ross T, et al. C3d regulates immune checkpoint blockade and enhances antitumor immunity. JCI Insight. 2017;2: pubmed publisher
product information
brand :
R&D Systems
master code :
AF2655
SKU :
AF2655
product name :
Mouse/Rat Complement Component C3d Antibody
unit size :
100 ug (also 25 ug)
seo description :
The Mouse/Rat Complement Component C3d Antibody from R&D Systems is a goat polyclonal antibody to Complement Component C3d. This antibody reacts with human,mouse,rat,transgenic mouse. The Mouse/Rat Complement Component C3d Antibody has been validated for the following applications: Immunocytochemistry,Western Blot,Immunohistochemistry,ELISA Development,ELISA Capture,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunofluorescence.
target :
Complement Component C3d
category :
Primary Antibodies
buffer :
Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.
clonality :
Polyclonal
concentration :
LYOPH
conjugate :
Unconjugated
dilution :
Western Blot 0.1 ug/mL, Immunohistochemistry 5-15 ug/mL
host :
Goat
immunogen :
E. coli -derived recombinant mouse Complement Component C3d, His1002-Arg1303 (Cys1010Ser), Accession # P01027
isotype :
IgG
purity :
Antigen Affinity-purified
species :
Human,Mouse,Rat,Transgenic Mouse
specificity :
Detects mouse Complement Component C3d in direct ELISAs and Western blots. Detects mouse and rat Complement Component C3d in immunohistochemistry.
top caption :
Complement Component C3d antibody in Mouse Kidney by Immunohistochemistry (IHC-Fr).
accessionNumbers :
P01027
applications :
ELISA Development,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunofluorescence,Immunocytochemistry,ELISA Capture
USD :
509 USD
alt names :
AHUS5;ARMD9;ASP;C3a;C3b;Complement C3;CPAMD1;HEL-S-62p
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.
more info or order :
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.