product summary
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company name :
R&D Systems
product type :
antibody
product name :
Human Osteoactivin/GPNMB Antibody
catalog :
AF2550
quantity :
100 ug (also 25 ug)
price :
519 USD
clonality :
polyclonal
host :
domestic goat
conjugate :
nonconjugated
reactivity :
human, mouse, rat
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, FACS, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, immunohistochemistry knockout validation
more info or order :
R&D Systems product webpage
citations: 30
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry knockout validation; mouse; 1:100; loading ...; fig 1c
Nickl B, Qadri F, Bader M. Anti-inflammatory role of Gpnmb in adipose tissue of mice. Sci Rep. 2021;11:19614 pubmed publisher
Yalcin F, Haneke H, Efe I, Kuhrt L, Motta E, Nickl B, et al. Tumor associated microglia/macrophages utilize GPNMB to promote tumor growth and alter immune cell infiltration in glioma. Acta Neuropathol Commun. 2024;12:50 pubmed publisher
Voshart D, Oshima T, Jiang Y, van der Linden G, Ainslie A, Reali Nazario L, et al. Radiotherapy induces persistent innate immune reprogramming of microglia into a primed state. Cell Rep. 2024;43:113764 pubmed publisher
Schmidt L, Vocke C, Ricketts C, Blake Z, Choo K, Nielsen D, et al. PRDM10 RCC: A Birt-Hogg-Dubé-like Syndrome Associated With Lipoma and Highly Penetrant, Aggressive Renal Tumors Morphologically Resembling Type 2 Papillary Renal Cell Carcinoma. Urology. 2023;179:58-70 pubmed publisher
Zhang T, Feng T, Wu K, Guo J, Nana A, Yang G, et al. Progranulin deficiency results in sex-dependent alterations in microglia in response to demyelination. Acta Neuropathol. 2023;146:97-119 pubmed publisher
Lang M, Schmidt L, Wilson K, Ricketts C, Sourbier C, Vocke C, et al. High-throughput and targeted drug screens identify pharmacological candidates against MiT-translocation renal cell carcinoma. J Exp Clin Cancer Res. 2023;42:99 pubmed publisher
Li Z, Belitzky E, Blaha O, Cavaliere A, Katz S, Aboian M, et al. ImmunoPET Imaging Identifies the Optimal Timepoint for Combination Therapy in Xenograft Models of Triple-Negative Breast Cancer. Cancers (Basel). 2023;15: pubmed publisher
Aubin R, Montelongo J, Hu R, Camara P. Clustering-independent estimation of cell abundances in bulk tissues using single-cell RNA-seq data. bioRxiv. 2023;: pubmed publisher
van de Beek I, Glykofridis I, Oosterwijk J, van den Akker P, Diercks G, Bolling M, et al. PRDM10 directs FLCN expression in a novel disorder overlapping with Birt-Hogg-Dubé syndrome and familial lipomatosis. Hum Mol Genet. 2023;32:1223-1235 pubmed publisher
Liu J, Ma P, Lai L, Villanueva A, Koenig A, Bean G, et al. Transcriptional and Immune Landscape of Cardiac Sarcoidosis. Circ Res. 2022;131:654-669 pubmed publisher
Sano T, Fukui T, Makita N, Shimizu K, Kono J, Masui K, et al. A novel missense mutation in the folliculin gene associated with the renal tumor-only phenotype of Birt-Hogg-Dubé syndrome. Cancer Genet. 2022;266-267:28-32 pubmed publisher
Winkler E, Kim C, Ross J, Garcia J, Gil E, Oh I, et al. A single-cell atlas of the normal and malformed human brain vasculature. Science. 2022;375:eabi7377 pubmed publisher
Harris P, Michel C, Yun Y, McGinnis C, Assiri M, Ahmadi A, et al. Proteomic analysis of alcohol-associated hepatitis reveals glycoprotein NMB (GPNMB) as a novel hepatic and serum biomarker. Alcohol. 2022;99:35-48 pubmed publisher
Qin W, Wang H, Zhong W, Bai J, Qiao J, Lin Z. Amyloidosis cutis dyschromica cases caused by GPNMB mutations with different inheritance patterns. J Dermatol Sci. 2021;104:48-54 pubmed publisher
Wang C, Okita Y, Zheng L, Shinkai Y, Manevich L, Chin J, et al. Glycoprotein non-metastatic melanoma protein B functions with growth factor signaling to induce tumorigenesis through its serine phosphorylation. Cancer Sci. 2021;112:4187-4197 pubmed publisher
Netanely D, Leibou S, Parikh R, Stern N, Vaknine H, Brenner R, et al. Classification of node-positive melanomas into prognostic subgroups using keratin, immune, and melanogenesis expression patterns. Oncogene. 2021;40:1792-1805 pubmed publisher
Glykofridis I, Knol J, Balk J, Westland D, Pham T, Piersma S, et al. Loss of FLCN-FNIP1/2 induces a non-canonical interferon response in human renal tubular epithelial cells. elife. 2021;10: pubmed publisher
Lang M, Vocke C, Ricketts C, Metwalli A, Ball M, Schmidt L, et al. Clinical and Molecular Characterization of Microphthalmia-associated Transcription Factor (MITF)-related Renal Cell Carcinoma. Urology. 2021;149:89-97 pubmed publisher
Huang M, Modeste E, Dammer E, Merino P, Taylor G, Duong D, et al. Network analysis of the progranulin-deficient mouse brain proteome reveals pathogenic mechanisms shared in human frontotemporal dementia caused by GRN mutations. Acta Neuropathol Commun. 2020;8:163 pubmed publisher
van Schaik J, Hanemaaijer S, Halmos G, Witjes M, van der Laan B, van der Vegt B, et al. Glycoprotein Nonmetastatic Melanoma Protein B as Potential Imaging Marker in Posttherapeutic Metastatic Head and Neck Cancer. Otolaryngol Head Neck Surg. 2020;163:1202-1208 pubmed publisher
Biswas K, Takahashi A, Mizutani Y, Takayama S, Ishitsuka A, Yang L, et al. GPNMB is expressed in human epidermal keratinocytes but disappears in the vitiligo lesional skin. Sci Rep. 2020;10:4930 pubmed publisher
Xie R, Okita Y, Ichikawa Y, Fikry M, Huynh Dam K, Tran S, et al. Role of the kringle-like domain in glycoprotein NMB for its tumorigenic potential. Cancer Sci. 2019;110:2237-2246 pubmed publisher
Baba M, Furuya M, Motoshima T, Lang M, Funasaki S, Ma W, et al. TFE3 Xp11.2 Translocation Renal Cell Carcinoma Mouse Model Reveals Novel Therapeutic Targets and Identifies GPNMB as a Diagnostic Marker for Human Disease. Mol Cancer Res. 2019;: pubmed publisher
Ashktorab H, Rahi H, Nouraie M, Shokrani B, Lee E, Haydari T, et al. GPNMB methylation: a new marker of potentially carcinogenic colon lesions. BMC Cancer. 2018;18:1068 pubmed publisher
Chen C, Okita Y, Watanabe Y, Abe F, Fikry M, Ichikawa Y, et al. Glycoprotein nmb Is Exposed on the Surface of Dormant Breast Cancer Cells and Induces Stem Cell-like Properties. Cancer Res. 2018;78:6424-6435 pubmed publisher
Ye X, Luke B, Wei B, Kaczmarczyk J, Loncarek J, Dwyer J, et al. Direct molecular dissection of tumor parenchyma from tumor stroma in tumor xenograft using mass spectrometry-based glycoproteomics. Oncotarget. 2018;9:26431-26452 pubmed publisher
Tajima J, Futamura M, Gaowa S, Mori R, Tanahashi T, Tanaka Y, et al. Clinical Significance of Glycoprotein Non-metastatic B and Its Association with EGFR/HER2 in Gastrointestinal Cancer. J Cancer. 2018;9:358-366 pubmed publisher
Yang C, Lin S, Chiang C, Wu Y, H ng W, Chang C, et al. Loss of GPNMB Causes Autosomal-Recessive Amyloidosis Cutis Dyschromica in Humans. Am J Hum Genet. 2018;102:219-232 pubmed publisher
Ma J, Baker A, Calabro A, Derwin K. Exploratory study on the effect of osteoactivin on muscle regeneration in a rat volumetric muscle loss model. PLoS ONE. 2017;12:e0175853 pubmed publisher
Nagahara Y, Shimazawa M, Ohuchi K, Ito J, Takahashi H, Tsuruma K, et al. GPNMB ameliorates mutant TDP-43-induced motor neuron cell death. J Neurosci Res. 2017;95:1647-1665 pubmed publisher
product information
master code :
AF2550
SKU :
AF2550
product name :
Human Osteoactivin/GPNMB Antibody
unit size :
100 ug (also 25 ug)
description :
The Human Osteoactivin/GPNMB Antibody from R&D Systems is a goat polyclonal antibody to Osteoactivin/GPNMB. This antibody reacts with human,mouse,rat,xenograft. The Human Osteoactivin/GPNMB Antibody has been validated for the following applications: Immunohistochemistry,Western Blot,Immunoprecipitation,Immunohistochemistry-Paraffin,FACS,Flow Cytometry,Immunohistochemistry-Frozen,Immunocytochemistry,ELISA Capture (Matched Antibody Pair).
target :
Osteoactivin/GPNMB
category :
Primary Antibodies
buffer :
Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.
clonality :
Polyclonal
concentration :
LYOPH
conjugate :
Unconjugated
host :
Goat
immunogen :
Mouse myeloma cell line NS0-derived recombinant human Osteoactivin isoform b, Lys23-Asn486, Accession # NP_002501
isotype :
IgG
purity :
Antigen Affinity-purified
species :
Human,Mouse,Rat,Xenograft
specificity :
Detects human Osteoactivin in ELISAs and Western blots. In sandwich immunoassays, less than 2% cross-reactivity with recombinant mouse Osteoactivin is observed.
gene symbol :
GPNMB
Antibody validation :
Knockout/Knockdown
top caption :
Detection of Human Osteoactivin/GPNMB antibody by Western Blot.
accessionNumbers :
NP_002501
applications :
Western Blot,Immunoprecipitation,Immunohistochemistry-Paraffin,Immunohistochemistry,FACS,Flow Cytometry,Immunohistochemistry-Frozen,Immunocytochemistry,ELISA Capture (Matched Antibody Pair)
USD :
519 USD
alt names :
DC-HIL, glycoprotein (transmembrane) nmb, glycoprotein NMB, GPNMB, HGFIN, HGFINglycoprotein nmb-like protein, NMBosteoactivin, Transmembrane glycoprotein HGFIN, transmembrane glycoprotein NMB
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.
more info or order :
R&D Systems product webpage
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.