product summary
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company name :
R&D Systems
product type :
antibody
product name :
Human PDX-1/IPF1 Antibody
catalog :
AF2419
quantity :
100 ug (also 25 ug)
price :
489 USD
clonality :
polyclonal
host :
domestic goat
conjugate :
nonconjugated
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
citations: 60
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; human; 1:250; fig 2d
Montaser H, Patel K, Balboa D, Ibrahim H, Lithovius V, Näätänen A, et al. Loss of MANF Causes Childhood-Onset Syndromic Diabetes Due to Increased Endoplasmic Reticulum Stress. Diabetes. 2021;70:1006-1018 pubmed publisher
Hogrebe N, Schmidt M, Augsornworawat P, Gale S, Shunkarova M, Millman J. Depolymerizing F-actin accelerates the exit from pluripotency to enhance stem cell-derived islet differentiation. bioRxiv. 2025;: pubmed publisher
Merz S, Sen xe9 e V, Philippi A, Oswald F, Shaigan M, F xfc hrer M, et al. A ONECUT1 regulatory, non-coding region in pancreatic development and diabetes. Cell Rep. 2024;43:114853 pubmed publisher
Lyu X, Rowley M, Kulik M, Dalton S, Corces V. Regulation of CTCF loop formation during pancreatic cell differentiation. Nat Commun. 2023;14:6314 pubmed publisher
Pollock S, Galicia Silva I, Liu M, Gruskin Z, Alvarez Dominguez J. Scalable generation of 3D pancreatic islet organoids from human pluripotent stem cells in suspension bioreactors. STAR Protoc. 2023;4:102580 pubmed publisher
Souralova T, Hulinova D, Jeseta M, Ventruba P, Hampl A, Koutna I. Truncated vitronectin with E-cadherin enables the xeno-free derivation of human embryonic stem cells. Sci Rep. 2023;13:15062 pubmed publisher
Li X, Ma Z, Lui K. A differentiation protocol for the generation of pancreatic beta-like cells from human embryonic stem cells. STAR Protoc. 2023;4:102407 pubmed publisher
Ito R, Kimura A, Hirose Y, Hatano Y, Mima A, Mae S, et al. Elucidation of HHEX in pancreatic endoderm differentiation using a human iPSC differentiation model. Sci Rep. 2023;13:8659 pubmed publisher
Hermann F, Kj xe6 rgaard M, Tian C, Tiemann U, Jackson A, Olsen L, et al. An insulin hypersecretion phenotype precedes pancreatic β cell failure in MODY3 patient-specific cells. Cell Stem Cell. 2022;: pubmed publisher
Souralova T, Rehakova D, Jeseta M, Tesarova L, Beranek J, Ventruba P, et al. The Manufacture of Xeno- and Feeder-Free Clinical-Grade Human Embryonic Stem Cell Lines: First Step for Cell Therapy. Int J Mol Sci. 2022;23: pubmed publisher
Beucher A, Miguel Escalada I, Balboa D, De Vas M, Maestro M, García Hurtado J, et al. The HASTER lncRNA promoter is a cis-acting transcriptional stabilizer of HNF1A. Nat Cell Biol. 2022;24:1528-1540 pubmed publisher
Nakashima Y, Miyagi Shiohira C, Saitoh I, Watanabe M, Matsushita M, Tsukahara M, et al. Induced hepatic stem cells are suitable for human hepatocyte production. iScience. 2022;25:105052 pubmed publisher
Sim E, Enomoto T, Shiraki N, Furuta N, Kashio S, Kambe T, et al. Methionine metabolism regulates pluripotent stem cell pluripotency and differentiation through zinc mobilization. Cell Rep. 2022;40:111120 pubmed publisher
Burda J, O Shea T, Ao Y, Suresh K, Wang S, Bernstein A, et al. Divergent transcriptional regulation of astrocyte reactivity across disorders. Nature. 2022;606:557-564 pubmed publisher
Ma H, de Zwaan E, Guo Y, Cejas P, Thiru P, van de Bunt M, et al. The nuclear receptor THRB facilitates differentiation of human PSCs into more mature hepatocytes. Cell Stem Cell. 2022;29:795-809.e11 pubmed publisher
Carrasco M, Wang C, S xf8 viknes A, Bj xf8 rlykke Y, Abadpour S, Paulo J, et al. Spatial Environment Affects HNF4A Mutation-Specific Proteome Signatures and Cellular Morphology in hiPSC-Derived β-Like Cells. Diabetes. 2022;71:862-869 pubmed publisher
Liu W, Flanders J, Wang L, Liu Q, Bowers D, Wang K, et al. A Safe, Fibrosis-Mitigating, and Scalable Encapsulation Device Supports Long-Term Function of Insulin-Producing Cells. Small. 2022;18:e2104899 pubmed publisher
Wu M, Lee M, Bahl V, Traum D, Schug J, Kusmartseva I, et al. Single-cell analysis of the human pancreas in type 2 diabetes using multi-spectral imaging mass cytometry. Cell Rep. 2021;37:109919 pubmed publisher
Merkle J, Breunig M, Schmid M, Allgöwer C, Krüger J, Melzer M, et al. CDKN2A-Mutated Pancreatic Ductal Organoids from Induced Pluripotent Stem Cells to Model a Cancer Predisposition Syndrome. Cancers (Basel). 2021;13: pubmed publisher
Aghazadeh Y, Poon F, Sarangi F, Wong F, Khan S, Sun X, et al. Microvessels support engraftment and functionality of human islets and hESC-derived pancreatic progenitors in diabetes models. Cell Stem Cell. 2021;28:1936-1949.e8 pubmed publisher
Hogrebe N, Maxwell K, Augsornworawat P, Millman J. Generation of insulin-producing pancreatic β cells from multiple human stem cell lines. Nat Protoc. 2021;16:4109-4143 pubmed publisher
Liu H, Li R, Liao H, Min Z, Wang C, Yu Y, et al. Chemical combinations potentiate human pluripotent stem cell-derived 3D pancreatic progenitor clusters toward functional β cells. Nat Commun. 2021;12:3330 pubmed publisher
Low B, Lim C, Ding S, Tan Y, Ng N, Krishnan V, et al. Decreased GLUT2 and glucose uptake contribute to insulin secretion defects in MODY3/HNF1A hiPSC-derived mutant β cells. Nat Commun. 2021;12:3133 pubmed publisher
Backx E, Wauters E, Baldan J, Van Bulck M, Michiels E, Heremans Y, et al. MECOM permits pancreatic acinar cell dedifferentiation avoiding cell death under stress conditions. Cell Death Differ. 2021;: pubmed publisher
Horton T, Kraemer B, Annes J. Protocol for determining zinc-dependent β cell-selective small-molecule delivery in mouse pancreas. STAR Protoc. 2021;2:100263 pubmed publisher
Yang K, Lee M, Jones P, Liu S, Zhou A, Xu J, et al. A 3D culture platform enables development of zinc-binding prodrugs for targeted proliferation of β cells. Sci Adv. 2020;6: pubmed publisher
Bernard H, Teijeiro A, Chaves Pérez A, Perna C, Satish B, Novials A, et al. Coxsackievirus B Type 4 Infection in β Cells Downregulates the Chaperone Prefoldin URI to Induce a MODY4-like Diabetes via Pdx1 Silencing. Cell Rep Med. 2020;1:100125 pubmed publisher
Ma H, Jeppesen J, Jaenisch R. Human T Cells Expressing a CD19 CAR-T Receptor Provide Insights into Mechanisms of Human CD19-Positive β Cell Destruction. Cell Rep Med. 2020;1:100097 pubmed publisher
Singh R, Cottle L, Loudovaris T, Xiao D, Yang P, Thomas H, et al. Enhanced structure and function of human pluripotent stem cell-derived beta-cells cultured on extracellular matrix. Stem Cells Transl Med. 2021;10:492-505 pubmed publisher
Augsornworawat P, Maxwell K, Velazco Cruz L, Millman J. Single-Cell Transcriptome Profiling Reveals β Cell Maturation in Stem Cell-Derived Islets after Transplantation. Cell Rep. 2020;32:108067 pubmed publisher
Dettmer R, Cirksena K, Münchhoff J, Kresse J, Diekmann U, Niwolik I, et al. FGF2 Inhibits Early Pancreatic Lineage Specification during Differentiation of Human Embryonic Stem Cells. Cells. 2020;9: pubmed publisher
Velazco Cruz L, Goedegebuure M, Maxwell K, Augsornworawat P, Hogrebe N, Millman J. SIX2 Regulates Human β Cell Differentiation from Stem Cells and Functional Maturation In Vitro. Cell Rep. 2020;31:107687 pubmed publisher
Loo L, Soetedjo A, Lau H, Ng N, Ghosh S, Nguyen L, et al. BCL-xL/BCL2L1 is a critical anti-apoptotic protein that promotes the survival of differentiating pancreatic cells from human pluripotent stem cells. Cell Death Dis. 2020;11:378 pubmed publisher
Helman A, Cangelosi A, Davis J, Pham Q, Rothman A, Faust A, et al. A Nutrient-Sensing Transition at Birth Triggers Glucose-Responsive Insulin Secretion. Cell Metab. 2020;31:1004-1016.e5 pubmed publisher
Blöchinger A, Siehler J, Wißmiller K, Shahryari A, Burtscher I, Lickert H. Generation of an INSULIN-H2B-Cherry reporter human iPSC line. Stem Cell Res. 2020;45:101797 pubmed publisher
Mahaddalkar P, Scheibner K, Pfluger S, Ansarullah -, Sterr M, Beckenbauer J, et al. Generation of pancreatic β cells from CD177+ anterior definitive endoderm. Nat Biotechnol. 2020;38:1061-1072 pubmed publisher
Kim S, Whitener R, Peiris H, Gu X, Chang C, Lam J, et al. Molecular and genetic regulation of pig pancreatic islet cell development. Development. 2020;147: pubmed publisher
Hogrebe N, Augsornworawat P, Maxwell K, Velazco Cruz L, Millman J. Targeting the cytoskeleton to direct pancreatic differentiation of human pluripotent stem cells. Nat Biotechnol. 2020;38:460-470 pubmed publisher
Wang Y, Lifshitz L, Gellatly K, Vinton C, Busman Sahay K, McCauley S, et al. HIV-1-induced cytokines deplete homeostatic innate lymphoid cells and expand TCF7-dependent memory NK cells. Nat Immunol. 2020;21:274-286 pubmed publisher
Alvarez Dominguez J, Donaghey J, Rasouli N, Kenty J, Helman A, Charlton J, et al. Circadian Entrainment Triggers Maturation of Human In Vitro Islets. Cell Stem Cell. 2020;26:108-122.e10 pubmed publisher
Seiron P, Wiberg A, Kuric E, Krogvold L, Jahnsen F, Dahl Jørgensen K, et al. Characterisation of the endocrine pancreas in type 1 diabetes: islet size is maintained but islet number is markedly reduced. J Pathol Clin Res. 2019;5:248-255 pubmed publisher
Augsornworawat P, Velazco Cruz L, Song J, Millman J. A hydrogel platform for in vitro three dimensional assembly of human stem cell-derived islet cells and endothelial cells. Acta Biomater. 2019;97:272-280 pubmed publisher
Lee K, Cho H, Rickert R, Li Q, Pulecio J, Leslie C, et al. FOXA2 Is Required for Enhancer Priming during Pancreatic Differentiation. Cell Rep. 2019;28:382-393.e7 pubmed publisher
Sharon N, Vanderhooft J, Straubhaar J, Mueller J, Chawla R, Zhou Q, et al. Wnt Signaling Separates the Progenitor and Endocrine Compartments during Pancreas Development. Cell Rep. 2019;27:2281-2291.e5 pubmed publisher
Rosado Olivieri E, Anderson K, Kenty J, Melton D. YAP inhibition enhances the differentiation of functional stem cell-derived insulin-producing β cells. Nat Commun. 2019;10:1464 pubmed publisher
Wang X, Sterr M, Ansarullah -, Burtscher I, Böttcher A, Beckenbauer J, et al. Point mutations in the PDX1 transactivation domain impair human β-cell development and function. Mol Metab. 2019;24:80-97 pubmed publisher
WANG Y, Traum D, Schug J, Gao L, Liu C, Atkinson M, et al. Multiplexed In Situ Imaging Mass Cytometry Analysis of the Human Endocrine Pancreas and Immune System in Type 1 Diabetes. Cell Metab. 2019;29:769-783.e4 pubmed publisher
Damond N, Engler S, Zanotelli V, Schapiro D, Wasserfall C, Kusmartseva I, et al. A Map of Human Type 1 Diabetes Progression by Imaging Mass Cytometry. Cell Metab. 2019;29:755-768.e5 pubmed publisher
Diekmann U, Wolling H, Dettmer R, Niwolik I, Naujok O, Buettner F. Chemically defined and xenogeneic-free differentiation of human pluripotent stem cells into definitive endoderm in 3D culture. Sci Rep. 2019;9:996 pubmed publisher
Zhou T, Kim T, Chong C, Tan L, Amin S, Sadat Badieyan Z, et al. A hPSC-based platform to discover gene-environment interactions that impact human β-cell and dopamine neuron survival. Nat Commun. 2018;9:4815 pubmed publisher
Ida H, Akiyama T, Ishiguro K, Goparaju S, Nakatake Y, Chikazawa Nohtomi N, et al. Establishment of a rapid and footprint-free protocol for differentiation of human embryonic stem cells into pancreatic endocrine cells with synthetic mRNAs encoding transcription factors. Stem Cell Res Ther. 2018;9:277 pubmed publisher
Wei R, Yuan F, Wu Y, Liu Y, You K, Yang Z, et al. Construction of a GLI3 compound heterozygous knockout human embryonic stem cell line WAe001-A-20 by CRISPR/Cas9 editing. Stem Cell Res. 2018;32:139-144 pubmed publisher
Tata P, Chow R, Saladi S, Tata A, Konkimalla A, Bara A, et al. Developmental History Provides a Roadmap for the Emergence of Tumor Plasticity. Dev Cell. 2018;44:679-693.e5 pubmed publisher
Robert T, De Mesmaeker I, Stangé G, Suenens K, Ling Z, Kroon E, et al. Functional Beta Cell Mass from Device-Encapsulated hESC-Derived Pancreatic Endoderm Achieving Metabolic Control. Stem Cell Reports. 2018;10:739-750 pubmed publisher
Cogger K, Sinha A, Sarangi F, McGaugh E, Saunders D, Dorrell C, et al. Glycoprotein 2 is a specific cell surface marker of human pancreatic progenitors. Nat Commun. 2017;8:331 pubmed publisher
Hosokawa Y, Toyoda T, Fukui K, Baden M, Funato M, Kondo Y, et al. Insulin-producing cells derived from 'induced pluripotent stem cells' of patients with fulminant type 1 diabetes: Vulnerability to cytokine insults and increased expression of apoptosis-related genes. J Diabetes Investig. 2017;: pubmed publisher
Liu H, Guo D, Ruzi A, Chen Y, Pan T, Yang F, et al. Testosterone improves the differentiation efficiency of insulin-producing cells from human induced pluripotent stem cells. PLoS ONE. 2017;12:e0179353 pubmed publisher
Navarro G, Abdolazimi Y, Zhao Z, Xu H, Lee S, Armstrong N, et al. Genetic Disruption of Adenosine Kinase in Mouse Pancreatic ?-Cells Protects Against High-Fat Diet-Induced Glucose Intolerance. Diabetes. 2017;66:1928-1938 pubmed publisher
Saarimäki Vire J, Balboa D, Russell M, Saarikettu J, Kinnunen M, Keskitalo S, et al. An Activating STAT3 Mutation Causes Neonatal Diabetes through Premature Induction of Pancreatic Differentiation. Cell Rep. 2017;19:281-294 pubmed publisher
Davenport C, Diekmann U, Budde I, Detering N, Naujok O. Anterior-Posterior Patterning of Definitive Endoderm Generated from Human Embryonic Stem Cells Depends on the Differential Signaling of Retinoic Acid, Wnt-, and BMP-Signaling. Stem Cells. 2016;34:2635-2647 pubmed publisher
product information
brand :
R&D Systems
master code :
AF2419
SKU :
AF2419
product name :
Human PDX-1/IPF1 Antibody
unit size :
100 ug (also 25 ug)
seo description :
The Human PDX-1/IPF1 Antibody from R&D Systems is a goat polyclonal antibody to PDX-1/IPF1. This antibody reacts with human,mouse,rat,xenograft. The Human PDX-1/IPF1 Antibody has been validated for the following applications: Immunocytochemistry,Immunohistochemistry,Flow Cytometry,Western Blot,Immunoprecipitation,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Simple Western,Dual RNAscope ISH-IHC Compatible.
target :
PDX-1/IPF1
category :
Primary Antibodies
buffer :
Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.
clonality :
Polyclonal
concentration :
LYOPH
conjugate :
Unconjugated
dilution :
Western Blot 1 ug/mL, Simple Western 10 ug/mL, Immunohistochemistry 5-15 ug/mL, Immunocytochemistry 5-15 ug/mL, Dual RNAscope ISH-IHC 5-15 ug/mL
host :
Goat
immunogen :
E. coli -derived recombinant human PDX-1/IPF1, Ala91-Arg283, Accession # P52945
isotype :
IgG
purity :
Antigen Affinity-purified
species :
Human,Mouse,Rat,Xenograft
specificity :
Detects human PDX-1/IPF1 in direct ELISAs and Western blots. In direct ELISAs, approximately 45% cross-reactivity with recombinant mouse PDX-1 is observed.
gene symbol :
PDX1
top caption :
Detection of Human PDX-1/IPF1 antibody by Western Blot.
accessionNumbers :
P52945
applications :
Simple Western,Dual RNAscope ISH-IHC Compatible,Flow Cytometry,Immunohistochemistry,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunocytochemistry
USD :
489 USD
alt names :
Glucose-sensitive factor, IDX1, IDX-1GSF, Insulin promoter factor 1, insulin promoter factor 1, homeodomain transcription factor, Insulin upstream factor 1, IPF1, IPF1pancreas/duodenum homeobox protein 1, Islet/duodenum homeobox-1, MODY4IUF1, pancreatic and duodenal homeobox 1, pancreatic-duodenal homeobox factor 1, PDX-1IPF-1, somatostatin transcription factor 1, Somatostatin-transactivating factor 1, STF-1IUF-1
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.
more info or order :
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.