product summary
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company name :
R&D Systems
product type :
antibody
product name :
Human EGFR Antibody
catalog :
AF231
quantity :
100 ug (also 25 ug)
price :
509 USD
clonality :
polyclonal
host :
domestic goat
conjugate :
nonconjugated
reactivity :
human, mouse
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
more info or order :
R&D Systems product webpage
citations: 17
Reference
Joshi M, Stanoev A, Huebinger J, Soetje B, Zorina V, Ro xdf mannek L, et al. The EGFR phosphatase RPTPγ is a redox-regulated suppressor of promigratory signaling. EMBO J. 2023;42:e111806 pubmed publisher
Ramos S, Mussa Z, Falk E, Pai B, Giotti B, Allette K, et al. An atlas of late prenatal human neurodevelopment resolved by single-nucleus transcriptomics. Nat Commun. 2022;13:7671 pubmed publisher
Beauvais D, Nelson S, Adams K, Stueven N, Jung O, Rapraeger A. Plasma membrane proteoglycans syndecan-2 and syndecan-4 engage with EGFR and RON kinase to sustain carcinoma cell cycle progression. J Biol Chem. 2022;298:102029 pubmed publisher
Peterson H, Chin L, Iwamoto Y, Oh J, Carlson J, Lee H, et al. Integrated Analytical System for Clinical Single-Cell Analysis. Adv Sci (Weinh). 2022;9:e2200415 pubmed publisher
Hu C, Leche C, Kiyatkin A, Yu Z, Stayrook S, Ferguson K, et al. Glioblastoma mutations alter EGFR dimer structure to prevent ligand bias. Nature. 2022;602:518-522 pubmed publisher
Yang L, Li Z, Liu G, Li X, Yang Z. Developmental Origins of Human Cortical Oligodendrocytes and Astrocytes. Neurosci Bull. 2022;38:47-68 pubmed publisher
Franco Nitta C, Green E, Jhamba E, Keth J, Ortiz Caraveo I, Grattan R, et al. EGFR transactivates RON to drive oncogenic crosstalk. elife. 2021;10: pubmed publisher
Egnuni T, Ingram N, Mirza I, Coletta P, McLaughlan J. Evaluation of the Targeting and Therapeutic Efficiency of Anti-EGFR Functionalised Nanoparticles in Head and Neck Cancer Cells for Use in NIR-II Optical Window. Pharmaceutics. 2021;13: pubmed publisher
Chakraborty R, Vickery K, Darido C, Ranganathan S, Hu H. Bacterial Antigens Reduced the Inhibition Effect of Capsaicin on Cal 27 Oral Cancer Cell Proliferation. Int J Mol Sci. 2021;22: pubmed publisher
Liu X, Adorno Cruz V, Chang Y, Jia Y, Kawaguchi M, Dashzeveg N, et al. EGFR inhibition blocks cancer stem cell clustering and lung metastasis of triple negative breast cancer. Theranostics. 2021;11:6632-6643 pubmed publisher
Sugita S, Yamato M, Hatabu T, Kataoka Y. Involvement of cancer-derived EMT cells in the accumulation of 18F-fluorodeoxyglucose in the hypoxic cancer microenvironment. Sci Rep. 2021;11:9668 pubmed publisher
Yogo N, Hase T, Kasama T, Nishiyama K, Ozawa N, Hatta T, et al. Development of an immuno-wall device for the rapid and sensitive detection of EGFR mutations in tumor tissues resected from lung cancer patients. PLoS ONE. 2020;15:e0241422 pubmed publisher
Zsengellér Z, Lo A, Tavasoli M, Pernicone E, Karumanchi S, Rosen S. Soluble fms-Like Tyrosine Kinase 1 Localization in Renal Biopsies of CKD. Kidney Int Rep. 2019;4:1735-1741 pubmed publisher
Dagnell M, Cheng Q, Rizvi S, Pace P, Boivin B, Winterbourn C, et al. Bicarbonate is essential for protein-tyrosine phosphatase 1B (PTP1B) oxidation and cellular signaling through EGF-triggered phosphorylation cascades. J Biol Chem. 2019;294:12330-12338 pubmed publisher
Haun F, Neumann S, Peintner L, Wieland K, Habicht J, Schwan C, et al. Identification of a novel anoikis signalling pathway using the fungal virulence factor gliotoxin. Nat Commun. 2018;9:3524 pubmed publisher
Klaesson A, Grannas K, Ebai T, Heldin J, Koos B, Leino M, et al. Improved efficiency of in situ protein analysis by proximity ligation using UnFold probes. Sci Rep. 2018;8:5400 pubmed publisher
Burke S, Zsengellér Z, Khankin E, Lo A, Rajakumar A, Dupont J, et al. Soluble fms-like tyrosine kinase 1 promotes angiotensin II sensitivity in preeclampsia. J Clin Invest. 2016;126:2561-74 pubmed publisher
product information
brand :
R&D Systems
master code :
AF231
SKU :
AF231
product name :
Human EGFR Antibody
unit size :
100 ug (also 25 ug)
seo description :
The Human EGFR Antibody from R&D Systems is a goat polyclonal antibody to EGFR. This antibody reacts with human,mouse. The Human EGFR Antibody has been validated for the following applications: Immunocytochemistry,Co-Immunoprecipitation,ELISA Development,ELISA Microarray Development,IHC/IF,Immunoprecipitation,Western Blot,ELISA Detection,Immunohistochemistry,Immunohistochemistry-Paraffin,Immunofluorescence,Flow Cytometry,Simple Western,ELISA Capture (Matched Antibody Pair),CyTOF-ready,Dual RNAscope ISH-IHC Compatible.
target :
EGFR
category :
Primary Antibodies
buffer :
Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.
clonality :
Polyclonal
concentration :
LYOPH
conjugate :
Unconjugated
dilution :
Western Blot 1 ug/mL, Simple Western 12.5 ug/mL, Flow Cytometry 0.25 ug/10^6 cells, Immunohistochemistry 1-15 ug/mL, Immunoprecipitation 1 ug/mL, Immunocytochemistry 1-15 ug/mL, CyTOF-ready, Dual RNAscope ISH-IHC 5-15 ug/mL, ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
host :
Goat
immunogen :
Mouse myeloma cell line NS0-derived recombinant human EGFR, Leu25-Ser645, Accession # CAA25240
isotype :
IgG
purity :
Antigen Affinity-purified
species :
Human,Mouse
specificity :
Detects human EGFR in ELISAs and Western blots. In sandwich ELISAs, approximately 3% cross-reactivity with recombinant mouse EGFR is observed and less than 0.1% cross-reactivity with recombinant human (rh) ErbB2 and rhErbB3 is observed.
gene symbol :
EGFR
top caption :
Detection of Human EGFR antibody by Western Blot.
accessionNumbers :
CAA25240
applications :
Flow Cytometry,Simple Western,ELISA Capture (Matched Antibody Pair),Immunocytochemistry,CyTOF-ready,Dual RNAscope ISH-IHC Compatible,Co-Immunoprecipitation,ELISA Development,ELISA Microarray Development,IHC/IF,Immunohistochemistry,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Immunofluorescence,ELISA Detection
USD :
509 USD
alt names :
avian erythroblastic leukemia viral (v-erb-b) oncogene homolog, cell growth inhibiting protein 40, cell proliferation-inducing protein 61, EC 2.7.10, EC 2.7.10.1, EGF R, epidermal growth factor receptor, epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b)oncogene homolog), ErbB, ErbB1, ERBB1PIG61, HER1, HER-1, mENA, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.
more info or order :
R&D Systems product webpage
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.