product summary
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company name :
R&D Systems
product type :
antibody
product name :
Mouse LDLR Antibody
catalog :
AF2255
quantity :
100 ug (also 25 ug)
price :
519 USD
clonality :
polyclonal
host :
domestic goat
conjugate :
nonconjugated
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry, immunocytochemistry, neutralization, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, western blot knockout validation
more info or order :
R&D Systems product webpage
citations: 30
Published Application/Species/Sample/DilutionReference
  • western blot knockout validation; mouse; 1:1000; loading ...; fig 3b
Susan Resiga D, Girard E, Essalmani R, Roubtsova A, Marcinkiewicz J, Derbali R, et al. Asialoglycoprotein receptor 1 is a novel PCSK9-independent ligand of liver LDLR cleaved by furin. J Biol Chem. 2021;297:101177 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:100; loading ...; fig 5d
Lebeau P, Byun J, Platko K, Saliba P, Sguazzin M, Macdonald M, et al. Caffeine blocks SREBP2-induced hepatic PCSK9 expression to enhance LDLR-mediated cholesterol clearance. Nat Commun. 2022;13:770 pubmed publisher
  • immunocytochemistry; mouse; loading ...; fig 7d
  • western blot; mouse; loading ...; fig 7b
Lv J, Wang H, Cui H, Liu Z, Zhang R, Lu M, et al. Blockade of Macrophage CD147 Protects Against Foam Cell Formation in Atherosclerosis. Front Cell Dev Biol. 2020;8:609090 pubmed publisher
Fowler A, Van Rompay K, Sampson M, Leo J, Watanabe J, Usachenko J, et al. A virus-like particle-based bivalent PCSK9 vaccine lowers LDL-cholesterol levels in non-human primates. NPJ Vaccines. 2023;8:142 pubmed publisher
Fowler A, Van Rompay K, Sampson M, Leo J, Watanabe J, Usachenko J, et al. A Virus-like particle-based bivalent PCSK9 vaccine lowers LDL-cholesterol levels in Non-Human Primates. bioRxiv. 2023;: pubmed publisher
Zhou Y, Wei J, Deng G, Hu A, Sun P, Zhao X, et al. Delivery of low-density lipoprotein from endocytic carriers to mitochondria supports steroidogenesis. Nat Cell Biol. 2023;25:937-949 pubmed publisher
Hjorth M, Doncheva A, Norheim F, Ulven S, Holven K, S xe6 ther T, et al. Consumption of salmon fishmeal increases hepatic cholesterol content in obese C57BL/6 J mice. Eur J Nutr. 2022;61:4027-4043 pubmed publisher
Gomes D, Wang S, Goodspeed L, Turk K, Wietecha T, Liu Y, et al. Comparison between genetic and pharmaceutical disruption of Ldlr expression for the development of atherosclerosis. J Lipid Res. 2022;63:100174 pubmed publisher
Louloudis G, Ambrosini S, Paneni F, Camici G, Benke D, Klohs J. Adeno-Associated Virus-Mediated Gain-of-Function mPCSK9 Expression in the Mouse Induces Hypercholesterolemia, Monocytosis, Neutrophilia, and a Hypercoagulative State. Front Cardiovasc Med. 2021;8:718741 pubmed publisher
Haberl E, Pohl R, Rein Fischboeck L, Höring M, Krautbauer S, Liebisch G, et al. Accumulation of cholesterol, triglycerides and ceramides in hepatocellular carcinomas of diethylnitrosamine injected mice. Lipids Health Dis. 2021;20:135 pubmed publisher
Feng J, Jiang W, Cheng X, Zou B, Varley A, Liu T, et al. A host lipase prevents lipopolysaccharide-induced foam cell formation. iScience. 2021;24:103004 pubmed publisher
Kabe Y, Koike I, Yamamoto T, Hirai M, Kanai A, Furuhata R, et al. Glycyrrhizin Derivatives Suppress Cancer Chemoresistance by Inhibiting Progesterone Receptor Membrane Component 1. Cancers (Basel). 2021;13: pubmed publisher
Shi Y, Andhey P, Ising C, Wang K, Snipes L, Boyer K, et al. Overexpressing low-density lipoprotein receptor reduces tau-associated neurodegeneration in relation to apoE-linked mechanisms. Neuron. 2021;109:2413-2426.e7 pubmed publisher
Kunii M, Noguchi Y, Yoshimura S, Kanda S, Iwano T, Avriyanti E, et al. SNAP23 deficiency causes severe brain dysplasia through the loss of radial glial cell polarity. J Cell Biol. 2021;220: pubmed publisher
Benmimoun B, Papastefanaki F, Perichon B, Segklia K, Roby N, Miriagou V, et al. An original infection model identifies host lipoprotein import as a route for blood-brain barrier crossing. Nat Commun. 2020;11:6106 pubmed publisher
Zhang C, Hao C, Shui G, Li W. BLOS1 mediates kinesin switch during endosomal recycling of LDL receptor. elife. 2020;9: pubmed publisher
Chella Krishnan K, Floyd R, Sabir S, Jayasekera D, Leon Mimila P, Jones A, et al. Liver Pyruvate Kinase Promotes NAFLD/NASH in Both Mice and Humans in a Sex-Specific Manner. Cell Mol Gastroenterol Hepatol. 2021;11:389-406 pubmed publisher
Garshick M, Baumer Y, Dey A, Grattan R, Ng Q, Teague H, et al. Characterization of PCSK9 in the Blood and Skin of Psoriasis. J Invest Dermatol. 2021;141:308-315 pubmed publisher
Pigoni M, Hsia H, Hartmann J, Rudan Njavro J, Shmueli M, Müller S, et al. Seizure protein 6 controls glycosylation and trafficking of kainate receptor subunits GluK2 and GluK3. EMBO J. 2020;39:e103457 pubmed publisher
Carrer A, Trefely S, Zhao S, Campbell S, Norgard R, Schultz K, et al. Acetyl-CoA Metabolism Supports Multistep Pancreatic Tumorigenesis. Cancer Discov. 2019;9:416-435 pubmed publisher
Khoshnejad M, Patel A, Wojtak K, Kudchodkar S, Humeau L, Lyssenko N, et al. Development of Novel DNA-Encoded PCSK9 Monoclonal Antibodies as Lipid-Lowering Therapeutics. Mol Ther. 2019;27:188-199 pubmed publisher
Fischer A, Albers K, Krott L, Hoffzimmer B, Heine M, Schmale H, et al. The adaptor protein PID1 regulates receptor-dependent endocytosis of postprandial triglyceride-rich lipoproteins. Mol Metab. 2018;16:88-99 pubmed publisher
Lebeau P, Platko K, Al Hashimi A, Byun J, Lhotak S, Holzapfel N, et al. Loss-of-function PCSK9 mutants evade the unfolded protein response sensor GRP78 and fail to induce endoplasmic reticulum stress when retained. J Biol Chem. 2018;293:7329-7343 pubmed publisher
Bornfeldt K, Kramer F, Batorsky A, Choi J, Hudkins K, Tontonoz P, et al. A Novel Type 2 Diabetes Mouse Model of Combined Diabetic Kidney Disease and Atherosclerosis. Am J Pathol. 2018;188:343-352 pubmed publisher
Peled M, Nishi H, Weinstock A, Barrett T, Zhou F, Quezada A, et al. A wild-type mouse-based model for the regression of inflammation in atherosclerosis. PLoS ONE. 2017;12:e0173975 pubmed publisher
Sirois F, Chretien M, Mbikay M. Comparing expression and activity of PCSK9 in SPRET/EiJ and C57BL/6J mouse strains shows lack of correlation with plasma cholesterol. Mol Genet Metab Rep. 2017;10:11-17 pubmed
Lebeau P, Al Hashimi A, Sood S, Lhotak S, Yu P, Gyulay G, et al. Endoplasmic Reticulum Stress and Ca2+ Depletion Differentially Modulate the Sterol Regulatory Protein PCSK9 to Control Lipid Metabolism. J Biol Chem. 2017;292:1510-1523 pubmed publisher
Pinkosky S, Newton R, Day E, Ford R, Lhotak S, Austin R, et al. Liver-specific ATP-citrate lyase inhibition by bempedoic acid decreases LDL-C and attenuates atherosclerosis. Nat Commun. 2016;7:13457 pubmed publisher
Kim S, Roy S, Chen B, Nguyen T, McMonigle R, McCracken A, et al. Targeting cancer metabolism by simultaneously disrupting parallel nutrient access pathways. J Clin Invest. 2016;126:4088-4102 pubmed publisher
Ramanathan A, Gusarova V, Stahl N, Gurnett Bander A, Kyratsous C. Alirocumab, a Therapeutic Human Antibody to PCSK9, Does Not Affect CD81 Levels or Hepatitis C Virus Entry and Replication into Hepatocytes. PLoS ONE. 2016;11:e0154498 pubmed publisher
product information
brand :
R&D Systems
master code :
AF2255
SKU :
AF2255
product name :
Mouse LDLR Antibody
unit size :
100 ug (also 25 ug)
seo description :
The Mouse LDLR Antibody from R&D Systems is a goat polyclonal antibody to LDLR. This antibody reacts with human,mouse,rat. The Mouse LDLR Antibody has been validated for the following applications: Western Blot,Blocking,ELISA Development,Flow Cytometry,Neutralization,Immunohistochemistry,Immunohistochemistry-Frozen,Immunocytochemistry,Blockade of Receptor-ligand Interaction,CyTOF-ready.
target :
LDLR
category :
Primary Antibodies
buffer :
Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.
clonality :
Polyclonal
concentration :
LYOPH
conjugate :
Unconjugated
dilution :
Western Blot 0.1 ug/mL, Flow Cytometry 2.5 ug/10^6 cells, Immunohistochemistry 5-15 ug/mL, Blockade of Receptor-ligand Interaction, CyTOF-ready
host :
Goat
immunogen :
Mouse myeloma cell line NS0-derived recombinant mouse LDL R, Ala22-Arg790 (Ala23Val, Cys27Gly), Accession # Q6GTJ9
isotype :
IgG
purity :
Antigen Affinity-purified
species :
Human,Mouse,Rat
specificity :
Detects mouse LDL R in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human LDL R is observed.
gene symbol :
LDLR
endotoxin note :
<0.10 EU per 1 ╡g of the antibody by the LAL method.
top caption :
Detection of Mouse LDLR antibody by Western Blot.
accessionNumbers :
Q6GTJ9
applications :
Blockade of Receptor-ligand Interaction,CyTOF-ready,Blocking,ELISA Development,Flow Cytometry,Immunohistochemistry,Western Blot,Immunohistochemistry-Frozen,Neutralization,Immunocytochemistry
USD :
519 USD
alt names :
FH, FHC, LDL R, LDL receptor, LDLCQ2, low density lipoprotein receptor, low-density lipoprotein receptor, low-density lipoprotein receptor class A domain-containing protein 3
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.
more info or order :
R&D Systems product webpage
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.