Human Legumain/Asparaginyl Endopeptidase Antibody | R&D Systems AF2199 product information

product summary

company name :

R&D Systems

product type :

antibody

product name :

Human Legumain/Asparaginyl Endopeptidase Antibody

catalog :

AF2199

quantity :

100 ug (also 25 ug)

price :

509 USD

clonality :

polyclonal

host :

domestic goat

conjugate :

nonconjugated

reactivity :

human, mouse, zebrafish

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

R&D Systems product webpage

citations: 18

Reference
Wang J, Yu H, Li X, Li F, Chen H, Zhang X, et al. A TrkB cleavage fragment in hippocampus promotes Depressive-Like behavior in mice. Brain Behav Immun. 2024;119:56-83 pubmed publisher
Xu X, Liu M, Peng K, Yu Y, Liu T. Asparaginyl endopeptidase contributes to cetuximab resistance via MEK/ERK signaling in RAS wide-type metastatic colorectal cancer. Clin Transl Oncol. 2023;25:776-785 pubmed publisher
Kovalyova Y, Bak D, Gordon E, Fung C, Shuman J, Cover T, et al. An infection-induced oxidation site regulates legumain processing and tumor growth. Nat Chem Biol. 2022;18:698-705 pubmed publisher
Clees A, Stolp V, Häupl B, Fuhrmann D, Wempe F, Seibert M, et al. Identification of the Cysteine Protease Legumain as a Potential Chronic Hypoxia-Specific Multiple Myeloma Target Gene. Cells. 2022;11: pubmed publisher
Wang Z, Xia Y, Wu Z, Kang S, Zhang J, Liu P, et al. Neuronal ApoE4 stimulates C/EBPβ activation, promoting Alzheimer's disease pathology in a mouse model. Prog Neurobiol. 2022;209:102212 pubmed publisher
Cao L, Xu C, Yi P, Li H, Lin Y, Cai G, et al. Asparaginyl endopeptidase (AEP) regulates myocardial apoptosis in response to radiation exposure via alterations in NRF2 activation. Am J Cancer Res. 2021;11:1206-1225 pubmed
Wang Z, Xia Y, Liu P, Liu X, Edgington Mitchell L, Lei K, et al. ApoE4 activates C/EBPβ/δ-secretase with 27-hydroxycholesterol, driving the pathogenesis of Alzheimer's disease. Prog Neurobiol. 2021;202:102032 pubmed publisher
Wang Y, Zhang S, Wang H, Cui Y, Wang Z, Cheng X, et al. High Level of Legumain Was Correlated With Worse Prognosis and Peritoneal Metastasis in Gastric Cancer Patients. Front Oncol. 2020;10:966 pubmed publisher
de Haan W, Øie C, Benkheil M, Dheedene W, Vinckier S, Coppiello G, et al. Unraveling the transcriptional determinants of liver sinusoidal endothelial cell specialization. Am J Physiol Gastrointest Liver Physiol. 2020;318:G803-G815 pubmed publisher
Schlegel K, Awwad K, Heym R, Holzinger D, Doell A, Barghorn S, et al. N368-Tau fragments generated by legumain are detected only in trace amount in the insoluble Tau aggregates isolated from AD brain. Acta Neuropathol Commun. 2019;7:177 pubmed publisher
Lin Y, Liao K, Miao Y, Qian Z, Fang Z, Yang X, et al. Role of Asparagine Endopeptidase in Mediating Wild-Type p53 Inactivation of Glioblastoma. J Natl Cancer Inst. 2020;112:343-355 pubmed publisher
Wang Z, Xiang J, Liu X, Yu S, Manfredsson F, Sandoval I, et al. Deficiency in BDNF/TrkB Neurotrophic Activity Stimulates δ-Secretase by Upregulating C/EBPβ in Alzheimer's Disease. Cell Rep. 2019;28:655-669.e5 pubmed publisher
Lunde N, Bosnjak T, Solberg R, Johansen H. Mammalian legumain - A lysosomal cysteine protease with extracellular functions?. Biochimie. 2019;: pubmed publisher
Chen X, Wang C, Liao K, Zhou S, Cao L, Chen J, et al. USP17 Suppresses Tumorigenesis and Tumor Growth through Deubiquitinating AEP. Int J Biol Sci. 2019;15:738-748 pubmed publisher
Liu X, Wang Z, Zhang G, Zhu Q, Zeng H, Wang T, et al. Overexpression of asparaginyl endopeptidase is significant for esophageal carcinoma metastasis and predicts poor patient prognosis. Oncol Lett. 2018;15:1229-1235 pubmed publisher
Yao P, Ding Y, Han Z, Mu Y, Hong T, Zhu Y, et al. Suppression of asparaginyl endopeptidase attenuates breast cancer-induced bone pain through inhibition of neurotrophin receptors. Mol Pain. 2017;13:1744806917708127 pubmed publisher
Lunde N, Haugen M, Bodin Larsen K, Damgaard I, Pettersen S, Kasem R, et al. Glycosylation is important for legumain localization and processing to active forms but not for cystatin E/M inhibitory functions. Biochimie. 2017;139:27-37 pubmed publisher
Hetland G, Eide D, Tangen J, Haugen M, Mirlashari M, Paulsen J. The Agaricus blazei-Based Mushroom Extract, Andosan™, Protects against Intestinal Tumorigenesis in the A/J Min/+ Mouse. PLoS ONE. 2016;11:e0167754 pubmed publisher

product information

brand :

R&D Systems

master code :

AF2199

SKU :

AF2199

product name :

Human Legumain/Asparaginyl Endopeptidase Antibody

unit size :

100 ug (also 25 ug)

seo description :

The Human Legumain/Asparaginyl Endopeptidase Antibody from R&D Systems is a goat polyclonal antibody to Legumain/Asparaginyl Endopeptidase. This antibody reacts with gerbil,human,mouse,transgenic mouse,fish - danio rerio (zebrafish). The Human Legumain/Asparaginyl Endopeptidase Antibody has been validated for the following applications: Western Blot,Immunohistochemistry,Immunoprecipitation,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Chromatin Immunoprecipitation (ChIP),Immunocytochemistry.

target :

Legumain/Asparaginyl Endopeptidase

category :

Primary Antibodies

buffer :

Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.

clonality :

Polyclonal

concentration :

LYOPH

conjugate :

Unconjugated

dilution :

Western Blot 1 ug/mL, Immunoprecipitation 25 ug/mL

host :

Goat

immunogen :

Mouse myeloma cell line NS0-derived recombinant human Legumain/Asparaginyl Endopeptidase, Ile18-Tyr433, Accession # Q99538

isotype :

IgG

purity :

Antigen Affinity-purified

species :

Gerbil,Human,Mouse,Transgenic Mouse,Fish - Danio rerio (Zebrafish)

specificity :

Detects human Legumain/Asparaginyl Endopeptidase in direct ELISAs and Western blots.

gene symbol :

LGMN

top caption :

Detection of Human Legumain/ Asparaginyl Endopeptidase antibody by Western Blot.

accessionNumbers :

Q99538

applications :

Immunohistochemistry,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Chromatin Immunoprecipitation (ChIP),Immunocytochemistry

USD :

509 USD

alt names :

AEP, Asparaginyl endopeptidase, cysteine protease 1, legumain, LGMN, LGMN1, Protease, cysteine 1, protease, cysteine, 1 (legumain), PRSC1EC 3.4.22.34

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage