product summary
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company name :
R&D Systems
product type :
antibody
product name :
Human Carbonic Anhydrase IX/CA9 Antibody
catalog :
AF2188
quantity :
100 ug (also 25 ug)
price :
509 USD
clonality :
polyclonal
host :
domestic goat
conjugate :
nonconjugated
reactivity :
human, mouse
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
more info or order :
R&D Systems product webpage
citations: 14
Reference
Fischer J, Jackson H, de Souza N, Varga Z, Schraml P, Moch H, et al. Multiplex imaging of breast cancer lymph node metastases identifies prognostic single-cell populations independent of clinical classifiers. Cell Rep Med. 2023;4:100977 pubmed publisher
De Vargas Roditi L, Jacobs A, Rueschoff J, Bankhead P, Chevrier S, Jackson H, et al. Single-cell proteomics defines the cellular heterogeneity of localized prostate cancer. Cell Rep Med. 2022;3:100604 pubmed publisher
Burgess E, Crake R, Phillips E, Morrin H, Royds J, Slatter T, et al. Increased Ascorbate Content of Glioblastoma Is Associated With a Suppressed Hypoxic Response and Improved Patient Survival. Front Oncol. 2022;12:829524 pubmed publisher
Lenferink A, McDonald P, Cantin C, Grothe S, Gosselin M, Baardsnes J, et al. Isolation and characterization of monoclonal antibodies against human carbonic anhydrase-IX. MAbs. 2021;13:1999194 pubmed publisher
Chafe S, Vizeacoumar F, Venkateswaran G, Nemirovsky O, Awrey S, Brown W, et al. Genome-wide synthetic lethal screen unveils novel CAIX-NFS1/xCT axis as a targetable vulnerability in hypoxic solid tumors. Sci Adv. 2021;7: pubmed publisher
Lefort S, El Naggar A, Tan S, Colborne S, Negri G, Pellacani D, et al. De novo and cell line models of human mammary cell transformation reveal an essential role for Yb-1 in multiple stages of human breast cancer. Cell Death Differ. 2021;: pubmed publisher
Bade R, Schehr J, Emamekhoo H, Gibbs B, Rodems T, Mannino M, et al. Development and initial clinical testing of a multiplexed circulating tumor cell assay in patients with clear cell renal cell carcinoma. Mol Oncol. 2021;15:2330-2344 pubmed publisher
Dachs G, Gandhi J, Wohlrab C, Carr A, Morrin H, Pullar J, et al. Vitamin C Administration by Intravenous Infusion Increases Tumor Ascorbate Content in Patients With Colon Cancer: A Clinical Intervention Study. Front Oncol. 2020;10:600715 pubmed publisher
Hedlund E, McDonald P, Nemirovsky O, Awrey S, Jensen L, Dedhar S. Harnessing Induced Essentiality: Targeting Carbonic Anhydrase IX and Angiogenesis Reduces Lung Metastasis of Triple Negative Breast Cancer Xenografts. Cancers (Basel). 2019;11: pubmed publisher
Wagner J, Rapsomaniki M, Chevrier S, Anzeneder T, Langwieder C, Dykgers A, et al. A Single-Cell Atlas of the Tumor and Immune Ecosystem of Human Breast Cancer. Cell. 2019;177:1330-1345.e18 pubmed publisher
Campbell E, Dachs G, Morrin H, Davey V, Robinson B, Vissers M. Activation of the hypoxia pathway in breast cancer tissue and patient survival are inversely associated with tumor ascorbate levels. BMC Cancer. 2019;19:307 pubmed publisher
Damond N, Engler S, Zanotelli V, Schapiro D, Wasserfall C, Kusmartseva I, et al. A Map of Human Type 1 Diabetes Progression by Imaging Mass Cytometry. Cell Metab. 2019;29:755-768.e5 pubmed publisher
Chevrier S, Crowell H, Zanotelli V, Engler S, Robinson M, Bodenmiller B. Compensation of Signal Spillover in Suspension and Imaging Mass Cytometry. Cell Syst. 2018;6:612-620.e5 pubmed publisher
Swayampakula M, McDonald P, Vallejo M, Coyaud E, Chafe S, Westerback A, et al. The interactome of metabolic enzyme carbonic anhydrase IX reveals novel roles in tumor cell migration and invadopodia/MMP14-mediated invasion. Oncogene. 2017;36:6244-6261 pubmed publisher
product information
brand :
R&D Systems
master code :
AF2188
SKU :
AF2188
product name :
Human Carbonic Anhydrase IX/CA9 Antibody
unit size :
100 ug (also 25 ug)
seo description :
The Human Carbonic Anhydrase IX/CA9 Antibody from R&D Systems is a goat polyclonal antibody to Carbonic Anhydrase IX/CA9. This antibody reacts with human,mouse,xenograft. The Human Carbonic Anhydrase IX/CA9 Antibody has been validated for the following applications: Immunocytochemistry,Immunohistochemistry,CyTof,Western Blot,Immunohistochemistry-Paraffin,Flow Cytometry,Immunoprecipitation,CyTOF-reported.
target :
Carbonic Anhydrase IX/CA9
category :
Primary Antibodies
buffer :
Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.
clonality :
Polyclonal
concentration :
LYOPH
conjugate :
Unconjugated
dilution :
Western Blot 1 ug/mL, Flow Cytometry 0.25 ug/10^6 cells, Immunohistochemistry 5-15 ug/mL, Immunoprecipitation 25 ug/mL, Immunocytochemistry 3-15 ug/mL, CyTOF-reported
host :
Goat
immunogen :
Mouse myeloma cell line NS0-derived recombinant human Carbonic Anhydrase IX, Pro59-Asp414, Accession # Q16790
isotype :
IgG
purity :
Antigen Affinity-purified
species :
Human,Mouse,Xenograft
specificity :
Detects human Carbonic Anhydrase IX (CA9) in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse CA9 is observed and less than 1% cross-reactivity with recombinant human CA1, 3, 4, 8, 10, 12, and 14 is observed.
gene symbol :
CA9
top caption :
Detection of Human Carbonic Anhydrase IX/CA9 antibody by Western Blot.
accessionNumbers :
Q16790
applications :
Flow Cytometry,Immunoprecipitation,CyTOF-reported,CyTof,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunocytochemistry
USD :
509 USD
alt names :
CA9, CAIX, CA-IX, Carbonate dehydratase IX, carbonic anhydrase 9, carbonic anhydrase IX, carbonic dehydratase, EC 4.2.1.1, G250, Membrane antigen MN, MN, P54/58N, PMW1, RCC, RCC-associated antigen G250, RCC-associated protein G250, Renal cell carcinoma-associated antigen G250
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.
more info or order :
R&D Systems product webpage
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.