product summary
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company name :
R&D Systems
product type :
antibody
product name :
Human/Mouse Brachyury Antibody
catalog :
AF2085
quantity :
100 ug (also 25 ug)
price :
509 USD
clonality :
polyclonal
host :
domestic goat
conjugate :
nonconjugated
reactivity :
human, mouse, chicken
application :
western blot, immunohistochemistry, immunocytochemistry, flow cytometry, chromatin immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
citations: 198
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; human; 1:100; loading ...; fig s1g
Yoo J, Lee D, Park S, Shin H, Lee K, Kim D, et al. Trophoblast glycoprotein is a marker for efficient sorting of ventral mesencephalic dopaminergic precursors derived from human pluripotent stem cells. NPJ Parkinsons Dis. 2021;7:61 pubmed publisher
  • immunohistochemistry - frozen section; chicken; 1:1000; fig 5m
Guillot C, Djeffal Y, Michaut A, Rabe B, Pourquie O. Dynamics of primitive streak regression controls the fate of neuromesodermal progenitors in the chicken embryo. elife. 2021;10: pubmed publisher
Huang C, Qiu L, Zhou W, Shao C, Wang X, Zhang Q, et al. A human-induced pluripotent stem cell (iPSC) line (SMUSHi006-A) from an ALS patient carrying a mutation c.1126C > T in the FUS gene. Stem Cell Res. 2025;82:103604 pubmed publisher
McCreery K, Stubb A, Stephens R, Fursova N, Cook A, Kruse K, et al. Mechano-osmotic signals control chromatin state and fate transitions in pluripotent stem cells. bioRxiv. 2024;: pubmed publisher
Despin Guitard E, Rosa V, Plunder S, Mathiah N, Van Schoor K, Nehme E, et al. Non-apical mitoses contribute to cell delamination during mouse gastrulation. Nat Commun. 2024;15:7364 pubmed publisher
Sun J, Ren L, Canel Rivero G, Xu L, Ladabaum U, C Wu J. Generation of two induced pluripotent stem cell lines from patients with Li-Fraumeni Syndrome carrying TP53 mutation. Stem Cell Res. 2024;81:103527 pubmed publisher
Qin H, Yu Y, Ye S, Alonzo M, Garg V, Zhao M. Generation of an induced pluripotent stem cell line (NCHi016-A) from a 5-year-old female with pulmonary atresia with intact ventricular septum and one-and-half ventricle palliation. Stem Cell Res. 2024;80:103530 pubmed publisher
Tang P, Keshi E, Wilken S, Wutsdorff L, Mougnekabol J, Pratschke J, et al. Generation of an induced pluripotent stem cell (iPSC) line (EXSURGi001-A) from a patient homozygous for the p.Ala165Thr mutation in the MTARC1 gene. Stem Cell Res. 2024;80:103516 pubmed publisher
Sekulovski N, Carleton A, Rengarajan A, Lin C, Juga L, Whorton A, et al. Temporally resolved single cell transcriptomics in a human model of amniogenesis. bioRxiv. 2024;: pubmed publisher
Juchem M, Lehmann N, Behrens Y, B xe4 r C, Thum T, Hoepfner J. CRISPR/Cas9-based GLA knockout to generate the female Fabry disease human induced pluripotent stem cell line MHHi001-A-15. Stem Cell Res. 2024;79:103478 pubmed publisher
Dai Y, Zhu W, Flores Banuelos A, Li J, Mukherjee S, Algaze C, et al. Generation of two induced pluripotent stem cell lines from patients with Williams syndrome. Stem Cell Res. 2024;78:103460 pubmed publisher
Wang J, Bering J, Alonzo M, Ye S, Texter K, Garg V, et al. Generation of iPSC line NCHi015-A from a patient with truncus arteriosus carrying heterozygous variants in KMT2D and NOTCH1. Stem Cell Res. 2024;78:103457 pubmed publisher
Tonin R, Feo F, Falliano S, Giunti L, Calamai M, Procopio E, et al. Generation of a human induced pluripotent stem cell line from a patient with GM3 synthase deficiency using self-replicating RNA vector. Stem Cell Res. 2024;77:103431 pubmed publisher
Jahn C, Juchem M, Sonnenschein K, Gietz A, Buchegger T, Lachmann N, et al. Generation of human induced pluripotent stem cell line MHHi029-A from a male Fabry disease patient carrying c.959A > T mutation. Stem Cell Res. 2024;77:103404 pubmed publisher
Weatherbee B, Weberling A, Gantner C, Iwamoto Stohl L, Barnikel Z, Barrie A, et al. Distinct pathways drive anterior hypoblast specification in the implanting human embryo. Nat Cell Biol. 2024;26:353-365 pubmed publisher
Daya N, D xf6 ring K, Zhuge H, Volke L, Stab V, Dietz J, et al. Generation of two hiPSCs lines of two patients carrying truncating mutations in the dimerization domain of filamin C. Stem Cell Res. 2024;76:103320 pubmed publisher
de Jong M, Adegeest E, B xe9 renger Currias N, Mircea M, Merks R, Semrau S. The shapes of elongating gastruloids are consistent with convergent extension driven by a combination of active cell crawling and differential adhesion. PLoS Comput Biol. 2024;20:e1011825 pubmed publisher
H xf6 pperger S, Spathopoulou A, Mayer Suess L, Suarez Cubero M, Sillaber K, Spreiz A, et al. Generation of the human induced pluripotent stem cell line (IBKMOLi003-A) from PBMCs of a vascular Ehlers-Danlos syndrome (vEDS) patient carrying the heterozygous nonsense mutation c.430C > T (p.Q105*) in the COL3A1 gene. Stem Cell Res. 2024;75:103321 pubmed publisher
Tripathi D, Manhas A, Noishiki C, Wu D, Adkar S, Sallam K, et al. Generation of induced pluripotent stem cell line from a patient suffering from arterial calcification due to deficiency of CD73 (ACDC). Stem Cell Res. 2024;75:103285 pubmed publisher
Tang M, Xiong M, Zhou W, Lei J, Huang M, Huang C, et al. Generation of a human induced pluripotent stem cell line (SMUSHi002-A) from an ALS patient carrying a heterozygous mutation c.1562G > A in the FUS gene. Stem Cell Res. 2024;74:103286 pubmed publisher
Park S, Park C, Eom J, Jo M, Hur H, Choi S, et al. Preclinical and dose-ranging assessment of hESC-derived dopaminergic progenitors for a clinical trial on Parkinson's disease. Cell Stem Cell. 2024;31:25-38.e8 pubmed publisher
Driver K, Vo C, Scriba C, Saker S, Larmonier T, Malfatti E, et al. Generation of two induced pluripotent stem cell lines from a 33-year-old central core disease patient with a heterozygous dominant c.14145_14156delCTACTGGGACA (p.Asn4715_Asp4718del) deletion in the RYR1 gene. Stem Cell Res. 2023;73:103258 pubmed publisher
Meyer K, Lammers N, Bugaj L, Garcia H, Weiner O. Optogenetic control of YAP reveals a dynamic communication code for stem cell fate and proliferation. Nat Commun. 2023;14:6929 pubmed publisher
Boeing A, Mavrommatis L, Daya N, Zhuge H, Volke L, Kocabas A, et al. Generation of two human iPSC lines (HIMRi002-A and HIMRi003-A) derived from Caveolinopathy patients with rippling muscle disease. Stem Cell Res. 2023;72:103220 pubmed publisher
Balducci V, Scardigli F, Harakalová M, Peter van Tintelen J, Doevendans P, Costa K, et al. Generation and characterization of novel human induced pluripotent stem cell (iPSC) lines originating from five asymptomatic individuals carrying the PLN-R14del pathogenic variant and a non-carrier relative. Stem Cell Res. 2023;72:103208 pubmed publisher
Liu W, Zeng W, Kong X, Htet M, Yu R, Wheeler M, et al. Generation of two induced pluripotent stem cell lines from Duchenne muscular dystrophy patients. Stem Cell Res. 2023;72:103207 pubmed publisher
Militi S, Nibhani R, Jalali M, Pauklin S. RBL2-E2F-GCN5 guide cell fate decisions during tissue specification by regulating cell-cycle-dependent fluctuations of non-cell-autonomous signaling. Cell Rep. 2023;42:113146 pubmed publisher
Karvas R, Zemke J, Ali S, Upton E, Sane E, Fischer L, et al. 3D-cultured blastoids model human embryogenesis from pre-implantation to early gastrulation stages. Cell Stem Cell. 2023;30:1148-1165.e7 pubmed publisher
Iwatsuki K, Oikawa M, Kobayashi H, Penfold C, Sanbo M, Yamamoto T, et al. Rat post-implantation epiblast-derived pluripotent stem cells produce functional germ cells. Cell Rep Methods. 2023;3:100542 pubmed publisher
Cunningham D, Stanberry I, Ye S, Alonzo M, Zhao M, Garg V, et al. Generation of iPSC line NCHi012-A from a patient with Alagille syndrome and heterozygous pathogenic variant in the JAG1 gene. Stem Cell Res. 2023;71:103177 pubmed publisher
Gattiglio M, Protzek M, Schr xf6 ter C. Population-level antagonism between FGF and BMP signaling steers mesoderm differentiation in embryonic stem cells. Biol Open. 2023;12: pubmed publisher
Yu Y, Alonzo M, Ye S, Fang A, Manickam K, Garg V, et al. Generation of an induced pluripotent stem cell line (NCHi010-A) from a 6-year-old female with Down syndrome and without congenital heart disease. Stem Cell Res. 2023;71:103155 pubmed publisher
Ukaji T, Takahashi Shibata M, Arai D, Tsutsumi H, Tajima S, Akamatsu W, et al. Generation and characterization of a human iPSC line (JUFMDOi007-A) from a patient with Usher syndrome due to mutation in USH2A. Stem Cell Res. 2023;69:103100 pubmed publisher
Zeng W, Kong X, Alamana C, Liu Y, Guzmán J, Pang P, et al. Generation of two induced pluripotent stem cell lines from spinal muscular atrophy type 1 patients carrying no functional copies of SMN1 gene. Stem Cell Res. 2023;69:103095 pubmed publisher
Schottmann N, Klug K, Klopocki E, xdc xe7 eyler N. Generation of induced pluripotent stem cell line (UKWNLi008) derived from a patient carrying a c.1678C>G variant in the transient receptor potential cation channel subfamily A member (TRPA1) gene potentially associated with small fiber neuropathy. Stem Cell Res. 2023;69:103094 pubmed publisher
Cossec J, Traboulsi T, Sart S, Loe Mie Y, Guthmann M, Hendriks I, et al. Transient suppression of SUMOylation in embryonic stem cells generates embryo-like structures. Cell Rep. 2023;42:112380 pubmed publisher
Garg V, Yang Y, Nowotschin S, Setty M, Kuo Y, Sharma R, et al. Single-cell analysis of bidirectional reprogramming between early embryonic states reveals mechanisms of differential lineage plasticities. bioRxiv. 2023;: pubmed publisher
Li J, Zhu Q, Cao J, Liu Y, Lu Y, Sun Y, et al. Cynomolgus monkey embryo model captures gastrulation and early pregnancy. Cell Stem Cell. 2023;30:362-377.e7 pubmed publisher
Almad A, Garcia L, Takanohashi A, Gagne A, Yang W, Ann McGuire J, et al. Generation of three induced Pluripotent Stem Cell lines from individuals with Hypomyelination with Atrophy of Basal Ganglia and Cerebellum caused by a c.745G>A (p.D249N) autosomal dominant mutation in TUBB4A. Stem Cell Res. 2023;69:103083 pubmed publisher
Kim A, Lee H, Kim H, Jung J, Seol H, Choi E, et al. Establishment of TUBB3-mCherry knock-in human pluripotent stem cell line using CRISPR/Cas9 (SNUe003-A-4). Stem Cell Res. 2023;69:103064 pubmed publisher
Fan Y, Hackland J, Baggiolini A, Hung L, Zhao H, Zumbo P, et al. hPSC-derived sacral neural crest enables rescue in a severe model of Hirschsprung's disease. Cell Stem Cell. 2023;30:264-282.e9 pubmed publisher
Luo Q, Pui H, Chen J, Yu L, Jannig P, Pei Y, et al. Epiblast-like stem cells established by Wnt/β-catenin signaling manifest distinct features of formative pluripotency and germline competence. Cell Rep. 2023;42:112021 pubmed publisher
Pilz R, Skowronek D, Mellinger L, Bekeschus S, Felbor U, Rath M. Endothelial Differentiation of CCM1 Knockout iPSCs Triggers the Establishment of a Specific Gene Expression Signature. Int J Mol Sci. 2023;24: pubmed publisher
Madrigal P, Deng S, Feng Y, Militi S, Goh K, Nibhani R, et al. Epigenetic and transcriptional regulations prime cell fate before division during human pluripotent stem cell differentiation. Nat Commun. 2023;14:405 pubmed publisher
Zushin P, Zhou Y, Li A, Ashley E, Wheeler M, Wu J. Generation of two human iPSC lines with Exon 3 mutations in BCL2-Associated Athanogene 3 (BAG3) from dilated cardiomyopathy patients. Stem Cell Res. 2023;67:103019 pubmed publisher
Klug K, Breyer M, Klopocki E, xdc xe7 eyler N. Generation of two induced pluripotent stem cell lines UKWNLi006 and UKWNLi007 derived from two patients with an active site GLA mutation leading to a pain and no pain phenotype in Fabry disease. Stem Cell Res. 2023;67:103025 pubmed publisher
Adhicary S, Ye S, Lin H, Texter K, Garg V, Zhao M. Establishment of NCHi009-A, an iPSC line from a patient with hypoplastic left heart syndrome (HLHS) carrying a heterozygous NOTCH1 mutation. Stem Cell Res. 2023;66:103013 pubmed publisher
Li L, Si X, Yang J, Lei M, Liu H, Ruan J, et al. Generation of a human iPSC line (CIBi014-A) from a patient with Parkinson's disease carrying a novel heterozygotic PARK8 (LRRK2) mutation. Stem Cell Res. 2023;66:102995 pubmed publisher
Treacy N, Clerkin S, Davis J, Kennedy C, Miller A, Saiani A, et al. Growth and differentiation of human induced pluripotent stem cell (hiPSC)-derived kidney organoids using fully synthetic peptide hydrogels. Bioact Mater. 2023;21:142-156 pubmed publisher
Pantazis C, Yang A, Lara E, McDonough J, Blauwendraat C, Peng L, et al. A reference human induced pluripotent stem cell line for large-scale collaborative studies. Cell Stem Cell. 2022;29:1685-1702.e22 pubmed publisher
Miller D, Lisowski P, Lickfett S, Mlody B, B xfc nning M, Genehr C, et al. Generation of induced pluripotent stem cells from three individuals with Huntington's disease. Stem Cell Res. 2022;65:102976 pubmed publisher
Chen I, Olshausen J, Thomas D, Lai C, McLaughlin T, Wu J. Generation of three induced pluripotent stem cell lines to model and investigate diseases affecting Hispanics. Stem Cell Res. 2022;65:102969 pubmed publisher
Prochazka L, Michaels Y, Lau C, Jones R, Siu M, Yin T, et al. Synthetic gene circuits for cell state detection and protein tuning in human pluripotent stem cells. Mol Syst Biol. 2022;18:e10886 pubmed publisher
Lin H, Ye S, Xu Z, Penaloza J, Aljuhani M, Vetter T, et al. Generation and characterization of a human induced pluripotent stem cell (iPSC) line from a patient with congenital heart disease (CHD). Stem Cell Res. 2022;65:102958 pubmed publisher
Gogolou A, Souilhol C, Granata I, Wymeersch F, Manipur I, Wind M, et al. Early anteroposterior regionalisation of human neural crest is shaped by a pro-mesodermal factor. elife. 2022;11: pubmed publisher
Bao M, Cornwall Scoones J, Sánchez Vásquez E, Chen D, De Jonghe J, Shadkhoo S, et al. Stem cell-derived synthetic embryos self-assemble by exploiting cadherin codes and cortical tension. Nat Cell Biol. 2022;24:1341-1349 pubmed publisher
Chen K, Martens Y, Meneses A, Ryu D, Lu W, Raulin A, et al. LRP1 is a neuronal receptor for α-synuclein uptake and spread. Mol Neurodegener. 2022;17:57 pubmed publisher
Cui G, Feng S, Yan Y, Wang L, He X, Li X, et al. Spatial molecular anatomy of germ layers in the gastrulating cynomolgus monkey embryo. Cell Rep. 2022;40:111285 pubmed publisher
Alonzo M, Contreras J, Ye S, Lin H, Hernandez Rosario L, McBride K, et al. Characterization of an iPSC line NCHi006-A from a patient with hypoplastic left heart syndrome (HLHS). Stem Cell Res. 2022;64:102892 pubmed publisher
Contreras J, Alonzo M, Ye S, Lin H, Hernandez Rosario L, McBride K, et al. Generation of an induced pluripotent stem cell line NCHi003-A from a 11-year-old male with pulmonary atresia with intact ventricular septum (PA-IVS). Stem Cell Res. 2022;64:102893 pubmed publisher
Jiang Z, Gu X, Su W, Duan Q, Lin J, Cao B, et al. Production of a human iPSC line from an early-onset Parkinson's disease patient with a novel CHCHD2 gene truncated mutation. Stem Cell Res. 2022;64:102881 pubmed publisher
Liu J, Black G, Kimber S, Sergouniotis P. Generation of a human induced pluripotent stem cell line carrying the TYR c.575C>A (p.Ser192Tyr) and c.1205G>A (p.Arg402Gln) variants in homozygous state using CRISPR-Cas9 genome editing. Stem Cell Res. 2022;64:102880 pubmed publisher
Gupta S, Kawaguchi R, Heinrichs E, Gallardo S, Castellanos S, Mandric I, et al. In vitro atlas of dorsal spinal interneurons reveals Wnt signaling as a critical regulator of progenitor expansion. Cell Rep. 2022;40:111119 pubmed publisher
Chen Y, Huang P, Wen M, Pan M, Lee D, Chen T. Generation of a homozygous knock-in human embryonic stem cell line expressing mEos4b-tagged CTR1. Stem Cell Res. 2022;63:102845 pubmed publisher
B xe9 renger Currias N, Mircea M, Adegeest E, van den Berg P, Feliksik M, Hochane M, et al. A gastruloid model of the interaction between embryonic and extra-embryonic cell types. J Tissue Eng. 2022;13:20417314221103042 pubmed publisher
Simunovic M, Siggia E, Brivanlou A. In vitro attachment and symmetry breaking of a human embryo model assembled from primed embryonic stem cells. Cell Stem Cell. 2022;29:962-972.e4 pubmed publisher
Shi Y, Wu S, Yang B, Yu B, Yan Y, Yang J, et al. Generation of a human iPSC line CIBi011-A from amniocytes of a healthy fetus. Stem Cell Res. 2022;62:102801 pubmed publisher
Fan Y, Zhao L, Lai Y, Lu K, Huang J. CRISPR-Cas9-mediated loss of function of β-catenin attenuates intervertebral disc degeneration. Mol Ther Nucleic Acids. 2022;28:387-396 pubmed publisher
Lee H, Kim A, Hwang S, Jung J, Seol H, Sung J, et al. Generation of αMHC-EGFP knock-in in human pluripotent stem cell line, SNUe003-A-3 using CRISPR/Cas9-based gene targeting. Stem Cell Res. 2022;61:102779 pubmed publisher
Manhas A, Jahng J, Vera C, Shenoy S, Knowles J, Wu J. Generation of two iPSC lines from hypertrophic cardiomyopathy patients carrying MYBPC3 and PRKAG2 variants. Stem Cell Res. 2022;61:102774 pubmed publisher
Hu X, Xiong S, Zhou X, Sun L. Generation of a human induced pluripotent stem cell line FMUPDCi001-A from a patient with mental retardation, autosomal recessive 36 (MRT36) carrying the variants c.219dupA and c.587C > T in ADAT3. Stem Cell Res. 2022;61:102777 pubmed publisher
Jo K, Teague S, Chen B, Khan H, Freeburne E, Li H, et al. Efficient differentiation of human primordial germ cells through geometric control reveals a key role for Nodal signaling. elife. 2022;11: pubmed publisher
Breyer M, Klein T, Klug K, Klopocki E, Uceyler N. Generation of the induced pluripotent stem cell line UKWNLi005-A derived from a patient with the GLA mutation c.376A > G of unknown pathogenicity in Fabry disease. Stem Cell Res. 2022;61:102747 pubmed publisher
Chiu W, Li A, Wang T, Li W, Zhang X. Generation of a MSX1 knockout human embryonic stem cell line using CRISPR/Cas9 technology. Stem Cell Res. 2022;60:102729 pubmed publisher
Lu V, Roy I, Torres A, Joly J, Ahsan F, Graham N, et al. Glutamine-dependent signaling controls pluripotent stem cell fate. Dev Cell. 2022;57:610-623.e8 pubmed publisher
Chumchuen S, Satirapod C, Tangprasittipap A, Hongeng S. Establishment of human induced pluripotent stem cell line MUi028-A from normal fetal skin fibroblasts. Stem Cell Res. 2022;60:102675 pubmed publisher
Ng W, Johnston E, Tan J, Bliley J, Feinberg A, Stolz D, et al. Recapitulating human cardio-pulmonary co-development using simultaneous multilineage differentiation of pluripotent stem cells. elife. 2022;11: pubmed publisher
Pour M, Kumar A, Farag N, Bolondi A, Kretzmer H, Walther M, et al. Emergence and patterning dynamics of mouse-definitive endoderm. iScience. 2022;25:103556 pubmed publisher
LeBlanc L, Kim M, Kambhampati A, Son A, Ramirez N, Kim J. β-catenin links cell seeding density to global gene expression during mouse embryonic stem cell differentiation. iScience. 2022;25:103541 pubmed publisher
Iannello G, Patel A, Sirabella D, Corneo B, Thaker V. Derivation and characterization of the induced pluripotent stem cell line CUIMCi004-A from a patient with a novel frameshift variant in exon 18a of OCRL. Stem Cell Res. 2021;59:102635 pubmed publisher
Rodina N, Khudiakov A, Perepelina K, Muravyev A, Boytsov A, Zlotina A, et al. Generation of iPSC line (FAMRCi009-A) from patient with familial progressive cardiac conduction disorder carrying genetic variant FLNC p.Val2264Met. Stem Cell Res. 2021;59:102640 pubmed publisher
Perepelina K, Khudiakov A, Rodina N, Boytsov A, Vavilova T, Zlotina A, et al. Generation of iPSC line FAMRCi010-A from patient with restrictive cardiomyopathy carrying genetic variant FLNC p.Gly2011Arg. Stem Cell Res. 2021;59:102639 pubmed publisher
Lee C, Cho S, Lai C, Shenoy S, Vagelos R, Wu J. Generation of three iPSC lines from dilated cardiomyopathy patients carrying a pathogenic LMNA variant. Stem Cell Res. 2021;59:102638 pubmed publisher
Belbachir N, Lai C, Rhee J, Zhuge Y, PEREZ M, Sallam K, et al. Generation of two induced pluripotent stem cell lines from Brugada syndrome affected patients carrying SCN5A mutations. Stem Cell Res. 2021;57:102605 pubmed publisher
Świerczek Lasek B, Dudka D, Bauer D, Czajkowski T, Ilach K, Stremińska W, et al. Comparison of Differentiation Pattern and WNT/SHH Signaling in Pluripotent Stem Cells Cultured under Different Conditions. Cells. 2021;10: pubmed publisher
Labouesse C, Tan B, Agley C, Hofer M, Winkel A, Stirparo G, et al. StemBond hydrogels control the mechanical microenvironment for pluripotent stem cells. Nat Commun. 2021;12:6132 pubmed publisher
Robert S, Flowers M, Ogle B. Kinases of the Focal Adhesion Complex Contribute to Cardiomyocyte Specification. Int J Mol Sci. 2021;22: pubmed publisher
Romanos M, Allio G, Roussigne M, Combres L, Escalas N, Soula C, et al. Cell-to-cell heterogeneity in Sox2 and Bra expression guides progenitor motility and destiny. elife. 2021;10: pubmed publisher
Scaramuzzino L, Lucchino V, Scalise S, Lo Conte M, Zannino C, Sacco A, et al. Uncovering the Metabolic and Stress Responses of Human Embryonic Stem Cells to FTH1 Gene Silencing. Cells. 2021;10: pubmed publisher
Jin G, Floy M, Simmons A, Arthur M, Palecek S. Spatial Stem Cell Fate Engineering via Facile Morphogen Localization. Adv Healthc Mater. 2021;10:e2100995 pubmed publisher
Mackinlay K, Weatherbee B, Souza Rosa V, Handford C, Hudson G, Coorens T, et al. An in vitro stem cell model of human epiblast and yolk sac interaction. elife. 2021;10: pubmed publisher
Shamshirgaran Y, Jonebring A, Svensson A, Leefa I, Bohlooly Y M, Firth M, et al. Rapid target validation in a Cas9-inducible hiPSC derived kidney model. Sci Rep. 2021;11:16532 pubmed publisher
Olmsted Z, Stigliano C, Scimemi A, Wolfe T, Cibelli J, Horner P, et al. Transplantable human motor networks as a neuron-directed strategy for spinal cord injury. iScience. 2021;24:102827 pubmed publisher
Liu B, Liu F, Gao X, Chen Y, Meng Q, Song Y, et al. Global Transcriptional Analyses of the Wnt-Induced Development of Neural Stem Cells from Human Pluripotent Stem Cells. Int J Mol Sci. 2021;22: pubmed publisher
Iannello G, Patel A, Sirabella D, Corneo B, Thaker V. Generation of the iPSC line CUIMCi003-A derived from a patient with severe early onset obesity. Stem Cell Res. 2021;54:102432 pubmed publisher
Ma L, Schmidt M, Morrow E. Human iPSC lines from a Christianson syndrome patient with NHE6 W523X mutation, a biologically-related control, and CRISPR/Cas9 gene-corrected isogenic controls. Stem Cell Res. 2021;54:102435 pubmed publisher
Martens Y, Xu S, Tait R, Li G, Zhao X, Lu W, et al. Generation and validation of APOE knockout human iPSC-derived cerebral organoids. STAR Protoc. 2021;2:100571 pubmed publisher
Huang P, Wen M, Xie X, Xu A, Lee D, Chen T. Generation of a homozygous knock-in human embryonic stem cell line expressing SNAP-tagged SOD1. Stem Cell Res. 2021;54:102415 pubmed publisher
Argenziano M, Burgos Angulo M, Najari Beidokhti M, Yang J, Bertalovitz A, McDonald T. Generation of a heterozygous FLNC mutation-carrying human iPSC line, USFi002-A, for modeling dilated cardiomyopathy. Stem Cell Res. 2021;53:102394 pubmed publisher
Beylina A, Langston R, Rosen D, Reed X, Cookson M. Generation of fourteen isogenic cell lines for Parkinson's disease-associated leucine-rich repeat kinase (LRRK2). Stem Cell Res. 2021;53:102354 pubmed publisher
Yang J, Samal E, Burgos Angulo M, Bertalovitz A, McDonald T. Establishment of an arrhythmogenic right ventricular cardiomyopathy derived iPSC cell line (USFi004-A) carrying a heterozygous mutation in PKP2 (c.1799delA). Stem Cell Res. 2021;54:102398 pubmed publisher
Yang J, Burgos Angulo M, Argenziano M, Bertalovitz A, Najari Beidokhti M, McDonald T. Generation of an iPSC cell line (USFi003-A) from a patient with dilated cardiomyopathy carrying a heterozygous mutation in LMNA (p.R541C). Stem Cell Res. 2021;54:102396 pubmed publisher
Ivanovitch K, Soro Barrio P, Chakravarty P, Jones R, Bell D, Mousavy Gharavy S, et al. Ventricular, atrial, and outflow tract heart progenitors arise from spatially and molecularly distinct regions of the primitive streak. PLoS Biol. 2021;19:e3001200 pubmed publisher
Morgani S, Su J, Nichols J, Massague J, Hadjantonakis A. The transcription factor Rreb1 regulates epithelial architecture, invasiveness, and vasculogenesis in early mouse embryos. elife. 2021;10: pubmed publisher
Sutcliffe D, Dinasarapu A, Visser J, Hoed J, Seifar F, Joshi P, et al. Induced pluripotent stem cells from subjects with Lesch-Nyhan disease. Sci Rep. 2021;11:8523 pubmed publisher
Lucchino V, Scaramuzzino L, Scalise S, Grillone K, Lo Conte M, Esposito C, et al. Generation of human induced pluripotent stem cell lines (UNIMGi003-A and UNIMGi004-A) from two Italian siblings affected by Unverricht-Lundborg disease. Stem Cell Res. 2021;53:102329 pubmed publisher
Ma L, Prada A, Schmidt M, Morrow E. Generation of pathogenic TPP1 mutations in human stem cells as a model for neuronal ceroid lipofuscinosis type 2 disease. Stem Cell Res. 2021;53:102323 pubmed publisher
Devito L, Healy L, Mohammed S, Guillemot F, Dias C. Generation of an iPSC line (CRICKi001-A) from an individual with a germline SMARCA4 missense mutation and autism spectrum disorder. Stem Cell Res. 2021;53:102304 pubmed publisher
Liu L, Shenoy S, Jahng J, Liu Y, Knowles J, Zhuge Y, et al. Generation of two heterozygous MYBPC3 mutation-carrying human iPSC lines, SCVIi001-A and SCVIi002-A, for modeling hypertrophic cardiomyopathy. Stem Cell Res. 2021;53:102279 pubmed publisher
Jahng J, Black K, Liu L, Bae H, Perez M, Ashley E, et al. Generation of three induced pluripotent stem cell lines, SCVIi003-A, SCVIi004-A, SCVIi005-A, from patients with ARVD/C caused by heterozygous mutations in the PKP2 gene. Stem Cell Res. 2021;53:102284 pubmed publisher
Sahni G, Chang S, Meng J, Tan J, Fatien J, Bonnard C, et al. A Micropatterned Human-Specific Neuroepithelial Tissue for Modeling Gene and Drug-Induced Neurodevelopmental Defects. Adv Sci (Weinh). 2021;8:2001100 pubmed publisher
Schmid B, Holst B, Clausen C, Bahnassawy L, Reinhardt P, Bakker M, et al. Generation of a set of isogenic iPSC lines carrying all APOE genetic variants (Ɛ2/Ɛ3/Ɛ4) and knock-out for the study of APOE biology in health and disease. Stem Cell Res. 2021;52:102180 pubmed publisher
Amadei G, Lau K, De Jonghe J, Gantner C, Sozen B, Chan C, et al. Inducible Stem-Cell-Derived Embryos Capture Mouse Morphogenetic Events In Vitro. Dev Cell. 2021;56:366-382.e9 pubmed publisher
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product information
brand :
R&D Systems
master code :
AF2085
SKU :
AF2085
product name :
Human/Mouse Brachyury Antibody
unit size :
100 ug (also 25 ug)
seo description :
The Human/Mouse Brachyury Antibody from R&D Systems is a goat polyclonal antibody to Brachyury. This antibody reacts with avian - chicken,chicken,human,mouse,primate - macaca fascicularis (crab-eating monkey or cynomolgus macaque),rat,transgenic mouse,xenograft. The Human/Mouse Brachyury Antibody has been validated for the following applications: Immunocytochemistry,Immunohistochemistry,IF/ICC,Flow Cytometry,IHC/IF,Immunolocalization,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,In vivo assay,Chromatin Immunoprecipitation (ChIP),Immunocytochemistry/ Immunofluorescence,Intracellular Staining by Flow Cytometry.
target :
Brachyury
category :
Primary Antibodies
buffer :
Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.
clonality :
Polyclonal
concentration :
LYOPH
conjugate :
Unconjugated
dilution :
Western Blot 0.1-1 ug/mL, Immunohistochemistry 5-15 ug/mL, Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells, Chromatin Immunoprecipitation (ChIP) 5 ug/10^6 cells, Immunocytochemistry 5-15 ug/mL
host :
Goat
immunogen :
E. coli -derived recombinant human Brachyury, Ser2-Glu202, Accession # O15178
isotype :
IgG
purity :
Antigen Affinity-purified
species :
Avian - Chicken,Chicken,Human,Mouse,Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque),Transgenic Mouse,Xenograft
specificity :
Detects human Brachyury in direct ELISAs and Western blots.
gene symbol :
T
top caption :
Detection of Recombinant Human Brachyury antibody by Western Blot.
accessionNumbers :
O15178
applications :
Intracellular Staining by Flow Cytometry,IF/ICC,IHC/IF,Immunolocalization,Flow Cytometry,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,In vivo assay,Chromatin Immunoprecipitation (ChIP),Immunocytochemistry,Immunocytochemistry/ Immunofluorescence
USD :
509 USD
alt names :
brachyury protein, MGC104817, Protein T, SAVA, T brachyury (mouse) homolog, T brachyury homolog, T Brachyury Transcription Factor, T, brachyury homolog (mouse), T-Box Transcription Factor T, TBXT, TF, TFT
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.
more info or order :
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.