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company name :
R&D Systems
product type :
antibody
product name :
Human VASA Antibody
catalog :
AF2030
quantity :
100 ug (also 25 ug)
price :
489 USD
clonality :
polyclonal
host :
domestic goat
conjugate :
nonconjugated
reactivity :
human, mouse, rhesus macaque
application :
western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
citations: 34
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; human; 1:500; fig 2a
Martin Inaraja M, Ferreira M, Taelman J, Eguizabal C, Chuva de Sousa Lopes S. Improving In Vitro Culture of Human Male Fetal Germ Cells. Cells. 2021;10: pubmed publisher
  • immunocytochemistry; human; 1:200; loading ...; fig 2b
Medrano J, Rombaut C, Simon C, Pellicer A, Goossens E. Human spermatogonial stem cells display limited proliferation in vitro under mouse spermatogonial stem cell culture conditions. Fertil Steril. 2016;106:1539-1549.e8 pubmed publisher
  • immunohistochemistry; human; 1:1000
Heeren A, He N, de Souza A, Goercharn Ramlal A, van Iperen L, Roost M, et al. On the development of extragonadal and gonadal human germ cells. Biol Open. 2016;5:185-94 pubmed publisher
Baskin L, Cao M, Li Y, Baker L, Cooper C, Cunha G. Ovotesticular cords and ovotesticular follicles: New histologic markers for human ovotesticular syndrome. J Pediatr Urol. 2024;20:799-809 pubmed publisher
Whiley P, Luu M, O Donnell L, Handelsman D, Loveland K. Testis exposure to unopposed/elevated activin A in utero affects somatic and germ cells and alters steroid levels mimicking phthalate exposure. Front Endocrinol (Lausanne). 2023;14:1234712 pubmed publisher
Bashawat M, Braun B, M xfc ller K, Hermann B. Molecular phenotyping of domestic cat (Felis catus) testicular cells across postnatal development - A model for wild felids. Theriogenology Wild. 2023;2: pubmed publisher
Overeem A, Chang Y, Moustakas I, Roelse C, Hillenius S, Helm T, et al. Efficient and scalable generation of primordial germ cells in 2D culture using basement membrane extract overlay. Cell Rep Methods. 2023;3:100488 pubmed publisher
Zhang X, Liu Y, Sosa F, Gunewardena S, Crawford P, Zielen A, et al. Transcriptional metabolic reprogramming implements meiotic fate decision in mouse testicular germ cells. Cell Rep. 2023;42:112749 pubmed publisher
Bailey A, Fuller M. YTHDC2 serves a distinct late role in spermatocytes during germ cell differentiation. bioRxiv. 2023;: pubmed publisher
Seita Y, Cheng K, McCarrey J, Yadu N, Cheeseman I, Bagwell A, et al. Efficient generation of marmoset primordial germ cell-like cells using induced pluripotent stem cells. elife. 2023;12: pubmed publisher
Zhao S, Huang C, Yang Y, Xu W, Yu Y, Wen C, et al. DNA repair protein FANCD2 has both ubiquitination-dependent and ubiquitination-independent functions during germ cell development. J Biol Chem. 2023;299:102905 pubmed publisher
Lehtiniemi T, Bourgery M, Ma L, Ahmedani A, M xe4 kel xe4 M, Asteljoki J, et al. SMG6 localizes to the chromatoid body and shapes the male germ cell transcriptome to drive spermatogenesis. Nucleic Acids Res. 2022;50:11470-11491 pubmed publisher
Moody S, Whiley P, Western P, Loveland K. The Impact of Activin A on Fetal Gonocytes: Chronic Versus Acute Exposure Outcomes. Front Endocrinol (Lausanne). 2022;13:896747 pubmed publisher
Mi X, Jiao W, Yang Y, Qin Y, Chen Z, Zhao S. HGF Secreted by Mesenchymal Stromal Cells Promotes Primordial Follicle Activation by Increasing the Activity of the PI3K-AKT Signaling Pathway. Stem Cell Rev Rep. 2022;18:1834-1850 pubmed publisher
Windley S, May xe8 re C, McGovern A, Harvey N, Nef S, Schwarz Q, et al. Loss of NEDD4 causes complete XY gonadal sex reversal in mice. Cell Death Dis. 2022;13:75 pubmed publisher
Kojima Y, Yamashiro C, Murase Y, Yabuta Y, Okamoto I, Iwatani C, et al. GATA transcription factors, SOX17 and TFAP2C, drive the human germ-cell specification program. Life Sci Alliance. 2021;4: pubmed publisher
Guo J, Sosa E, Chitiashvili T, Nie X, Rojas E, Oliver E, et al. Single-cell analysis of the developing human testis reveals somatic niche cell specification and fetal germline stem cell establishment. Cell Stem Cell. 2021;28:764-778.e4 pubmed publisher
Shetty G, Mitchell J, Meyer J, Wu Z, Lam T, Phan T, et al. Restoration of functional sperm production in irradiated pubertal rhesus monkeys by spermatogonial stem cell transplantation. Andrology. 2020;8:1428-1441 pubmed publisher
Bagheri Fam S, Chen H, Wilson S, Ayers K, Hughes J, Sloan Béna F, et al. The gene encoding the ketogenic enzyme HMGCS2 displays a unique expression during gonad development in mice. PLoS ONE. 2020;15:e0227411 pubmed publisher
Laurentino S, Heckmann L, Di Persio S, Li X, Meyer zu Horste G, Wistuba J, et al. High-resolution analysis of germ cells from men with sex chromosomal aneuploidies reveals normal transcriptome but impaired imprinting. Clin Epigenetics. 2019;11:127 pubmed publisher
Kojima M, de Rooij D, Page D. Amplification of a broad transcriptional program by a common factor triggers the meiotic cell cycle in mice. elife. 2019;8: pubmed publisher
Shetty G, Wu Z, Lam T, Phan T, Orwig K, Meistrich M. Effect of hormone modulations on donor-derived spermatogenesis or colonization after syngeneic and xenotransplantation in mice. Andrology. 2019;7:257-265 pubmed publisher
Hermann B, Cheng K, Singh A, Roa De La Cruz L, Mutoji K, Chen I, et al. The Mammalian Spermatogenesis Single-Cell Transcriptome, from Spermatogonial Stem Cells to Spermatids. Cell Rep. 2018;25:1650-1667.e8 pubmed publisher
Stringer J, Forster S, Qu Z, Prokopuk L, O Bryan M, Gardner D, et al. Reduced PRC2 function alters male germline epigenetic programming and paternal inheritance. BMC Biol. 2018;16:104 pubmed publisher
Serra N, Velte E, Niedenberger B, Kirsanov O, Geyer C. The mTORC1 component RPTOR is required for maintenance of the foundational spermatogonial stem cell pool in mice†. Biol Reprod. 2019;100:429-439 pubmed publisher
Vértesy Á, Arindrarto W, Roost M, Reinius B, Torrens Juaneda V, Bialecka M, et al. Parental haplotype-specific single-cell transcriptomics reveal incomplete epigenetic reprogramming in human female germ cells. Nat Commun. 2018;9:1873 pubmed publisher
Bothun A, Gao Y, Takai Y, Ishihara O, Seki H, Karger B, et al. Quantitative proteomic profiling of the human ovary from early to mid-gestation reveals protein expression dynamics of oogenesis and folliculogenesis. Stem Cells Dev. 2018;: pubmed publisher
Gell J, Zhao J, Chen D, Hunt T, Clark A. PRDM14 is expressed in germ cell tumors with constitutive overexpression altering human germline differentiation and proliferation. Stem Cell Res. 2018;27:46-56 pubmed publisher
Wang N, Satirapod C, Ohguchi Y, Park E, Woods D, Tilly J. Genetic studies in mice directly link oocytes produced during adulthood to ovarian function and natural fertility. Sci Rep. 2017;7:10011 pubmed publisher
Clark A, Gkountela S, Chen D, Liu W, Sosa E, Sukhwani M, et al. Primate Primordial Germ Cells Acquire Transplantation Potential by Carnegie Stage 23. Stem Cell Reports. 2017;9:329-341 pubmed publisher
Eguizabal C, Herrera L, de Oñate L, Montserrat N, Hajkova P, Izpisua Belmonte J. Characterization of the Epigenetic Changes During Human Gonadal Primordial Germ Cells Reprogramming. Stem Cells. 2016;34:2418-28 pubmed publisher
Fereydouni B, Salinas Riester G, Heistermann M, Dressel R, Lewerich L, Drummer C, et al. Long-Term Oocyte-Like Cell Development in Cultures Derived from Neonatal Marmoset Monkey Ovary. Stem Cells Int. 2016;2016:2480298 pubmed publisher
Hu Y, Nicholls P, Soh Y, Daniele J, Junker J, van Oudenaarden A, et al. Licensing of primordial germ cells for gametogenesis depends on genital ridge signaling. PLoS Genet. 2015;11:e1005019 pubmed publisher
Heeren A, van Iperen L, Klootwijk D, De Melo Bernardo A, Roost M, Gomes Fernandes M, et al. Development of the follicular basement membrane during human gametogenesis and early folliculogenesis. BMC Dev Biol. 2015;15:4 pubmed publisher
product information
master code :
AF2030
SKU :
AF2030
product name :
Human VASA Antibody
unit size :
100 ug (also 25 ug)
description :
The Human VASA Antibody from R&D Systems is a goat polyclonal antibody to VASA. This antibody reacts with human,monkey,mouse,primate - macaca mulatta (rhesus macaque),transgenic mouse,xenograft. The Human VASA Antibody has been validated for the following applications: Immunohistochemistry,Immunocytochemistry,Flow Cytometry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunofluorescence,Immunocytochemistry/ Immunofluorescence.
target :
VASA
category :
Primary Antibodies
buffer :
Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.
clonality :
Polyclonal
concentration :
LYOPH
conjugate :
Unconjugated
host :
Goat
immunogen :
E. coli -derived recombinant human VASA, Met1-Tyr145, Accession # Q9NQI0
isotype :
IgG
purity :
Antigen Affinity-purified
species :
Human,Mouse,Primate - Macaca mulatta (Rhesus Macaque)
specificity :
Detects human VASA in direct ELISAs and Western blots.
gene symbol :
DDX4
top caption :
Detection of Human VASA antibody by Western Blot.
accessionNumbers :
Q9NQI0
applications :
Immunocytochemistry,Immunohistochemistry,Flow Cytometry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunofluorescence,Immunocytochemistry/ Immunofluorescence
USD :
489 USD
alt names :
DDX4, DEAD (Asp-Glu-Ala-Asp) box polypeptide 4, DEAD box protein 4, EC 3.6.1, EC 3.6.4.13, MGC111074, probable ATP-dependent RNA helicase DDX4, Vasa homolog, VASADEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.
more info or order :
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.