product summary
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company name :
R&D Systems
product type :
antibody
product name :
Human Otx2 Antibody
catalog :
AF1979
quantity :
100 ug (also 25 ug)
price :
499 USD
clonality :
polyclonal
host :
domestic goat
conjugate :
nonconjugated
reactivity :
human, mouse, chicken, chimpanzee, zebrafish
application :
western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, immunohistochemistry knockout validation
more info or order :
citations: 147
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry knockout validation; mouse; loading ...; fig 1a
Pensieri P, Mantilleri A, Plassard D, Furukawa T, Moya K, Prochiantz A, et al. Photoreceptor cKO of OTX2 Enhances OTX2 Intercellular Transfer in the Retina and Causes Photophobia. Eneuro. 2021;8: pubmed publisher
  • immunocytochemistry; human; 1:2000; loading ...; fig s1c
Aldrin Kirk P, Akerblom M, Cardoso T, Nolbrant S, Adler A, Liu X, et al. A novel two-factor monosynaptic TRIO tracing method for assessment of circuit integration of hESC-derived dopamine transplants. Stem Cell Reports. 2021;: pubmed publisher
  • immunohistochemistry - frozen section; chicken; 1:500; loading ...; fig 3a
Chen X, Emerson M. Notch signaling represses cone photoreceptor formation through the regulation of retinal progenitor cell states. Sci Rep. 2021;11:14525 pubmed publisher
  • immunocytochemistry; human; 1:2000; loading ...
Nilsson F, Storm P, Sozzi E, Hidalgo Gil D, Birtele M, Sharma Y, et al. Single-Cell Profiling of Coding and Noncoding Genes in Human Dopamine Neuron Differentiation. Cells. 2021;10: pubmed publisher
Sun J, Ren L, Canel Rivero G, Xu L, Ladabaum U, C Wu J. Generation of two induced pluripotent stem cell lines from patients with Li-Fraumeni Syndrome carrying TP53 mutation. Stem Cell Res. 2024;81:103527 pubmed publisher
Qin H, Yu Y, Ye S, Alonzo M, Garg V, Zhao M. Generation of an induced pluripotent stem cell line (NCHi016-A) from a 5-year-old female with pulmonary atresia with intact ventricular septum and one-and-half ventricle palliation. Stem Cell Res. 2024;80:103530 pubmed publisher
Bianchini L, Sieber L, Hammad R, Sch xe4 fer R, Kutscher L. Generation of two isogenic patient-derived human-induced pluripotent stem cell clones with 6q27 deletion. Stem Cell Res. 2024;80:103524 pubmed publisher
Tang P, Keshi E, Wilken S, Wutsdorff L, Mougnekabol J, Pratschke J, et al. Generation of an induced pluripotent stem cell (iPSC) line (EXSURGi001-A) from a patient homozygous for the p.Ala165Thr mutation in the MTARC1 gene. Stem Cell Res. 2024;80:103516 pubmed publisher
Schumacher S, Fernkorn M, Marten M, Chen R, Kim Y, Bedzhov I, et al. Tissue-intrinsic beta-catenin signals antagonize Nodal-driven anterior visceral endoderm differentiation. Nat Commun. 2024;15:5055 pubmed publisher
Dai Y, Zhu W, Flores Banuelos A, Li J, Mukherjee S, Algaze C, et al. Generation of two induced pluripotent stem cell lines from patients with Williams syndrome. Stem Cell Res. 2024;78:103460 pubmed publisher
Wang J, Bering J, Alonzo M, Ye S, Texter K, Garg V, et al. Generation of iPSC line NCHi015-A from a patient with truncus arteriosus carrying heterozygous variants in KMT2D and NOTCH1. Stem Cell Res. 2024;78:103457 pubmed publisher
Yarkova E, Grigor eva E, Medvedev S, Tarasevich D, Pavlova S, Valetdinova K, et al. Detection of ER Stress in iPSC-Derived Neurons Carrying the p.N370S Mutation in the GBA1 Gene. Biomedicines. 2024;12: pubmed publisher
Weatherbee B, Weberling A, Gantner C, Iwamoto Stohl L, Barnikel Z, Barrie A, et al. Distinct pathways drive anterior hypoblast specification in the implanting human embryo. Nat Cell Biol. 2024;26:353-365 pubmed publisher
Daya N, D xf6 ring K, Zhuge H, Volke L, Stab V, Dietz J, et al. Generation of two hiPSCs lines of two patients carrying truncating mutations in the dimerization domain of filamin C. Stem Cell Res. 2024;76:103320 pubmed publisher
Tripathi D, Manhas A, Noishiki C, Wu D, Adkar S, Sallam K, et al. Generation of induced pluripotent stem cell line from a patient suffering from arterial calcification due to deficiency of CD73 (ACDC). Stem Cell Res. 2024;75:103285 pubmed publisher
Park S, Park C, Eom J, Jo M, Hur H, Choi S, et al. Preclinical and dose-ranging assessment of hESC-derived dopaminergic progenitors for a clinical trial on Parkinson's disease. Cell Stem Cell. 2024;31:25-38.e8 pubmed publisher
Feng L, Li D, Tian Y, Zhao C, Sun Y, Kou X, et al. One-step cell biomanufacturing platform: porous gelatin microcarrier beads promote human embryonic stem cell-derived midbrain dopaminergic progenitor cell differentiation in vitro and survival after transplantation in vivo. Neural Regen Res. 2024;19:458-464 pubmed publisher
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Driver K, Vo C, Scriba C, Saker S, Larmonier T, Malfatti E, et al. Generation of two induced pluripotent stem cell lines from a 33-year-old central core disease patient with a heterozygous dominant c.14145_14156delCTACTGGGACA (p.Asn4715_Asp4718del) deletion in the RYR1 gene. Stem Cell Res. 2023;73:103258 pubmed publisher
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Liu W, Zeng W, Kong X, Htet M, Yu R, Wheeler M, et al. Generation of two induced pluripotent stem cell lines from Duchenne muscular dystrophy patients. Stem Cell Res. 2023;72:103207 pubmed publisher
Iwatsuki K, Oikawa M, Kobayashi H, Penfold C, Sanbo M, Yamamoto T, et al. Rat post-implantation epiblast-derived pluripotent stem cells produce functional germ cells. Cell Rep Methods. 2023;3:100542 pubmed publisher
Jourdon A, Wu F, Mariani J, Capauto D, Norton S, Tomasini L, et al. Modeling idiopathic autism in forebrain organoids reveals an imbalance of excitatory cortical neuron subtypes during early neurogenesis. Nat Neurosci. 2023;26:1505-1515 pubmed publisher
Cunningham D, Stanberry I, Ye S, Alonzo M, Zhao M, Garg V, et al. Generation of iPSC line NCHi012-A from a patient with Alagille syndrome and heterozygous pathogenic variant in the JAG1 gene. Stem Cell Res. 2023;71:103177 pubmed publisher
Girgin M, Broguiere N, Mattolini L, Lutolf M. A New Approach to Generate Gastruloids to Develop Anterior Neural Tissues. Bio Protoc. 2023;13:e4722 pubmed publisher
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Yu Y, Alonzo M, Ye S, Fang A, Manickam K, Garg V, et al. Generation of an induced pluripotent stem cell line (NCHi010-A) from a 6-year-old female with Down syndrome and without congenital heart disease. Stem Cell Res. 2023;71:103155 pubmed publisher
Nishimura K, xc1 sgr xed msd xf3 ttir E, Yang S, Arenas E. A protocol for the differentiation of human embryonic stem cells into midbrain dopaminergic neurons. STAR Protoc. 2023;4:102355 pubmed publisher
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Zeng W, Kong X, Alamana C, Liu Y, Guzmán J, Pang P, et al. Generation of two induced pluripotent stem cell lines from spinal muscular atrophy type 1 patients carrying no functional copies of SMN1 gene. Stem Cell Res. 2023;69:103095 pubmed publisher
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Almad A, Garcia L, Takanohashi A, Gagne A, Yang W, Ann McGuire J, et al. Generation of three induced Pluripotent Stem Cell lines from individuals with Hypomyelination with Atrophy of Basal Ganglia and Cerebellum caused by a c.745G>A (p.D249N) autosomal dominant mutation in TUBB4A. Stem Cell Res. 2023;69:103083 pubmed publisher
Shigyo M, Kobayashi Y, Platoshyn O, Marsala S, Kato T, Takamura N, et al. Derivation of Sendai-Virus-Reprogrammed Human iPSCs-Neuronal Precursors: In Vitro and In Vivo Post-grafting Safety Characterization. Cell Transplant. 2023;32:9636897231163232 pubmed publisher
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Grigor eva E, Kopytova A, Yarkova E, Pavlova S, Sorogina D, Malakhova A, et al. Biochemical Characteristics of iPSC-Derived Dopaminergic Neurons from N370S GBA Variant Carriers with and without Parkinson's Disease. Int J Mol Sci. 2023;24: pubmed publisher
Mainwaring O, Weishaupt H, Zhao M, Ros xe9 n G, Borgenvik A, Breinschmid L, et al. ARF suppression by MYC but not MYCN confers increased malignancy of aggressive pediatric brain tumors. Nat Commun. 2023;14:1221 pubmed publisher
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Zushin P, Zhou Y, Li A, Ashley E, Wheeler M, Wu J. Generation of two human iPSC lines with Exon 3 mutations in BCL2-Associated Athanogene 3 (BAG3) from dilated cardiomyopathy patients. Stem Cell Res. 2023;67:103019 pubmed publisher
Adhicary S, Ye S, Lin H, Texter K, Garg V, Zhao M. Establishment of NCHi009-A, an iPSC line from a patient with hypoplastic left heart syndrome (HLHS) carrying a heterozygous NOTCH1 mutation. Stem Cell Res. 2023;66:103013 pubmed publisher
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product information
brand :
R&D Systems
master code :
AF1979
SKU :
AF1979
product name :
Human Otx2 Antibody
unit size :
100 ug (also 25 ug)
seo description :
The Human Otx2 Antibody from R&D Systems is a goat polyclonal antibody to Otx2. This antibody reacts with avian - chicken,human,mouse,porcine,primate - macaca fascicularis (crab-eating monkey or cynomolgus macaque),primate - pan troglodytes (chimpanzee),rat,transgenic mouse,fish - danio rerio (zebrafish),fish - oryzias latipes (medaka or japanese rice fish). The Human Otx2 Antibody has been validated for the following applications: Immunocytochemistry,Immunohistochemistry,Bioassay,IHC/IF,Differentiation,Flow Cytometry,Western Blot,Cell Culture,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunofluorescence,Simple Western.
target :
Otx2
category :
Primary Antibodies
buffer :
Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.
clonality :
Polyclonal
concentration :
LYOPH
conjugate :
Unconjugated
dilution :
Western Blot 1 ug/mL, Simple Western 10 ug/mL, Immunohistochemistry 1-15 ug/mL, Immunocytochemistry 5-15 ug/mL
host :
Goat
immunogen :
E. coli -derived recombinant human Otx2, Met1-Leu289, Accession # P32243
isotype :
IgG
purity :
Antigen Affinity-purified
species :
Avian - Chicken,Human,Mouse,Porcine,Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque),Primate - Pan troglodytes (Chimpanzee),Rat,Transgenic Mouse,Fish - Danio rerio (Zebrafish),Fish - Oryzias latipes (Medaka or Japanese Rice Fish)
specificity :
Detects human Otx2 in direct ELISAs and Western blots.
gene symbol :
OTX2
top caption :
Detection of Human Otx2 antibody by Western Blot.
accessionNumbers :
P32243
applications :
Simple Western,Bioassay,IHC/IF,Differentiation,Flow Cytometry,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunofluorescence,Immunocytochemistry,Cell Culture
USD :
499 USD
alt names :
homeobox protein OTX2, MCOPS5, MGC45000, orthodenticle homeobox 2, Orthodenticle homolog 2, orthodenticle homolog 2 (Drosophila), OTX2
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.
more info or order :
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.