Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody | R&D Systems AF1935 product information

product summary

company name :

R&D Systems

product type :

antibody

product name :

Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody

catalog :

AF1935

quantity :

100 ug (also 25 ug)

price :

509 USD

clonality :

polyclonal

host :

domestic goat

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation, immunohistochemistry - frozen section

more info or order :

R&D Systems product webpage

citations: 29

Published Application/Species/Sample/Dilution	Reference
western blot; human; loading ...; fig 4e, 5e	Fan X, Zhao Z, Song J, Zhang D, Wu F, Tu J, et al. LncRNA-SNHG6 promotes the progression of hepatocellular carcinoma by targeting miR-6509-5p and HIF1A. Cancer Cell Int. 2021;21:150 pubmed publisher
immunocytochemistry; human; 1:20; loading ...; fig s4	Liu M, Li N, Qu C, Gao Y, Wu L, Hu L. Amylin deposition activates HIF1α and 6-phosphofructo-2-kinase/fructose-2, 6-biphosphatase 3 (PFKFB3) signaling in failing hearts of non-human primates. Commun Biol. 2021;4:188 pubmed publisher
	Sanmarco L, Rone J, Polonio C, Fernandez Lahore G, Giovannoni F, Ferrara K, et al. Lactate limits CNS autoimmunity by stabilizing HIF-1α in dendritic cells. Nature. 2023;620:881-889 pubmed publisher
	Cornuault L, H xe9 rion F, Bourguignon C, Rouault P, Foussard N, Alzieu P, et al. Partial Mural Cell Ablation Disrupts Coronary Vasculature Integrity and Induces Systolic Dysfunction. J Am Heart Assoc. 2023;12:e029279 pubmed publisher
	Aoki Y, Dai H, Furuta F, Akamatsu T, Oshima T, Takahashi N, et al. LOX-1 mediates inflammatory activation of microglial cells through the p38-MAPK/NF-κB pathways under hypoxic-ischemic conditions. Cell Commun Signal. 2023;21:126 pubmed publisher
	Sanmarco L, Rone J, Polonio C, Giovannoni F, Lahore G, Ferrara K, et al. Engineered probiotics limit CNS autoimmunity by stabilizing HIF-1α in dendritic cells. bioRxiv. 2023;: pubmed publisher
	Militaru I, Rus A, Munteanu C, Manica G, Petrescu S. New panel of biomarkers to discriminate between amelanotic and melanotic metastatic melanoma. Front Oncol. 2022;12:1061832 pubmed publisher
	Li P, Shi D, Jin T, Tang D, Wang W, Wang L. MTA1 aggravates experimental colitis in mice by promoting transcription factor HIF1A and up-regulating AQP4 expression. Cell Death Discov. 2022;8:298 pubmed publisher
	Burgess E, Crake R, Phillips E, Morrin H, Royds J, Slatter T, et al. Increased Ascorbate Content of Glioblastoma Is Associated With a Suppressed Hypoxic Response and Improved Patient Survival. Front Oncol. 2022;12:829524 pubmed publisher
	Kjeld T, Isbrand A, Linnet K, Zerahn B, Højberg J, Hansen E, et al. Extreme Hypoxia Causing Brady-Arrythmias During Apnea in Elite Breath-Hold Divers. Front Physiol. 2021;12:712573 pubmed publisher
	Zeng G, Wang T, Zhang J, Kang Y, Feng L. FLI1 mediates the selective expression of hypoxia-inducible factor 1 target genes in endothelial cells under hypoxic conditions. FEBS Open Bio. 2021;: pubmed publisher
	Balukoff N, Ho J, Theodoridis P, Wang M, Bokros M, Llanio L, et al. A translational program that suppresses metabolism to shield the genome. Nat Commun. 2020;11:5755 pubmed publisher
	Di Marco B, Crouch E, Shah B, Duman C, Paredes M, Ruiz de Almodovar C, et al. Reciprocal Interaction between Vascular Filopodia and Neural Stem Cells Shapes Neurogenesis in the Ventral Telencephalon. Cell Rep. 2020;33:108256 pubmed publisher
	Felsenstein M, Blank A, Bungert A, Mueller A, Ghori A, Kremenetskaia I, et al. CCR2 of Tumor Microenvironmental Cells Is a Relevant Modulator of Glioma Biology. Cancers (Basel). 2020;12: pubmed publisher
	Arias Cavieres A, Khuu M, Nwakudu C, Barnard J, Dalgin G, Garcia A. A HIF1a-Dependent Pro-Oxidant State Disrupts Synaptic Plasticity and Impairs Spatial Memory in Response to Intermittent Hypoxia. Eneuro. 2020;7: pubmed publisher
	Becker L, O Connell J, Vo A, Cain M, Tampe D, Bizarro L, et al. Epigenetic Reprogramming of Cancer-Associated Fibroblasts Deregulates Glucose Metabolism and Facilitates Progression of Breast Cancer. Cell Rep. 2020;31:107701 pubmed publisher
	Ho J, Balukoff N, Theodoridis P, Wang M, Krieger J, Schatz J, et al. A network of RNA-binding proteins controls translation efficiency to activate anaerobic metabolism. Nat Commun. 2020;11:2677 pubmed publisher
	Li X, Zhao Y, Chen C, Yang L, Lee H, Wang Z, et al. Critical Role of Matrix Metalloproteinase 14 in Adipose Tissue Remodeling during Obesity. Mol Cell Biol. 2020;40: pubmed publisher
	Tawara K, Scott H, Emathinger J, Ide A, Fox R, Greiner D, et al. Co-Expression of VEGF and IL-6 Family Cytokines is Associated with Decreased Survival in HER2 Negative Breast Cancer Patients: Subtype-Specific IL-6 Family Cytokine-Mediated VEGF Secretion. Transl Oncol. 2019;12:245-255 pubmed publisher
	Billin A, Honeycutt S, McDougal A, Kerr J, Chen Z, Freudenberg J, et al. HIF prolyl hydroxylase inhibition protects skeletal muscle from eccentric contraction-induced injury. Skelet Muscle. 2018;8:35 pubmed publisher
	Yang Q, Xu J, Ma Q, Liu Z, Sudhahar V, Cao Y, et al. PRKAA1/AMPKα1-driven glycolysis in endothelial cells exposed to disturbed flow protects against atherosclerosis. Nat Commun. 2018;9:4667 pubmed publisher
	Liu X, Zhang W, Wu Z, Yang Y, Kang Y. Copper levels affect targeting of hypoxia-inducible factor 1α to the promoters of hypoxia-regulated genes. J Biol Chem. 2018;293:14669-14677 pubmed publisher
	Liu Z, Yan S, Wang J, Xu Y, Wang Y, Zhang S, et al. Endothelial adenosine A2a receptor-mediated glycolysis is essential for pathological retinal angiogenesis. Nat Commun. 2017;8:584 pubmed publisher
	Li L, Zviti R, Ha C, Wang Z, Hill J, Lin F. Forkhead box O3 (FoxO3) regulates kidney tubular autophagy following urinary tract obstruction. J Biol Chem. 2017;292:13774-13783 pubmed publisher
	Li X, Lu J, Kan Q, Li X, Fan Q, Li Y, et al. Metabolic reprogramming is associated with flavopiridol resistance in prostate cancer DU145 cells. Sci Rep. 2017;7:5081 pubmed publisher
	Himmels P, Paredes I, Adler H, Karakatsani A, Luck R, Marti H, et al. Motor neurons control blood vessel patterning in the developing spinal cord. Nat Commun. 2017;8:14583 pubmed publisher
	Campbell E, Vissers M, Dachs G. Ascorbate availability affects tumor implantation-take rate and increases tumor rejection in Gulo-/- mice. Hypoxia (Auckl). 2016;4:41-52 pubmed
	Quaranta M, Spinello I, Paolillo R, Macchia G, Boe A, Ceccarini M, et al. Identification of β-Dystrobrevin as a Direct Target of miR-143: Involvement in Early Stages of Neural Differentiation. PLoS ONE. 2016;11:e0156325 pubmed publisher
	Lopez Haber C, Barrio Real L, Casado Medrano V, Kazanietz M. Heregulin/ErbB3 Signaling Enhances CXCR4-Driven Rac1 Activation and Breast Cancer Cell Motility via Hypoxia-Inducible Factor 1α. Mol Cell Biol. 2016;36:2011-26 pubmed publisher

product information

brand :

R&D Systems

master code :

AF1935

SKU :

AF1935

product name :

Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody

unit size :

100 ug (also 25 ug)

seo description :

The Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody from R&D Systems is a goat polyclonal antibody to HIF-1 alpha. This antibody reacts with human,mouse,rat,transgenic mouse. The Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody has been validated for the following applications: Immunohistochemistry,Western Blot,IHC/IF,IHC-F,ChIP (chromatin immunoprecipit,Simple Western,Direct ELISA,Chromatin Immunoprecipitation (ChIP),Immunocytochemistry,Immunoprecipitation,Knockout Validated.

target :

HIF-1 alpha/HIF1A

category :

Primary Antibodies

buffer :

Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.

clonality :

Polyclonal

concentration :

LYOPH

conjugate :

Unconjugated

dilution :

Western Blot 0.5 ug/mL, Simple Western 5 ug/mL, Immunoprecipitation 2 ug/500 ug cell lysate, Chromatin Immunoprecipitation (ChIP) 5 ug/5 x 10^6 cells, Immunocytochemistry 0.3-15 ug/mL, Knockout Validated 0.3-15 ug/mL

host :

Goat

immunogen :

E. coli -derived recombinant human HIF-1 alpha /HIF1A, Arg575-Asn826, Accession # Q16665.1

isotype :

IgG

purity :

Antigen Affinity-purified

species :

Human,Mouse,Rat,Transgenic Mouse

specificity :

Detects human, mouse and rat HIF-1 alpha /HIF1A.

gene symbol :

HIF1A

top caption :

Detection of Human HIF-1a/HIF1A antibody by Western Blot.

accessionNumbers :

Q16665.1

applications :

Immunoprecipitation,Knockout Validated,IHC/IF,IHC-F,ChIP (chromatin immunoprecipit,Immunohistochemistry,Western Blot,Simple Western,Direct ELISA,Chromatin Immunoprecipitation (ChIP),Immunocytochemistry

USD :

509 USD

alt names :

AINT, anti-HIF-1 alpha, anti-HIF1A, ARNT interacting protein, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, BHLHE78, Class E basic helix-loop-helix protein 78, HIF 1A, HIF1, HIF1 alpha, HIF-1 alpha, HIF-1a, HIF1A, HIF-1alpha, HIF-1-alpha, HIF1-alpha, hypoxia inducible factor 1 alpha subunit, hypoxia inducible factor 1 subunit alpha, hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor), hypoxia-inducible factor 1-alpha, Member of PAS protein 1, member of PAS superfamily 1, MOP1, PAS domain-containing protein 8, PASD8

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage