product summary
Loading...
company name :
R&D Systems
product type :
antibody
product name :
Mouse Lipocalin-2/NGAL Antibody
catalog :
AF1857
quantity :
100 ug (also 25 ug)
price :
499 USD
clonality :
polyclonal
host :
domestic goat
conjugate :
nonconjugated
reactivity :
human, mouse
application :
western blot, immunohistochemistry, immunocytochemistry, neutralization, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, immunohistochemistry - free floating section
more info or order :
citations: 71
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - free floating section; mouse; 1:200; loading ...; fig s12a
O Shea T, Ao Y, Wang S, Wollenberg A, Kim J, Ramos Espinoza R, et al. Lesion environments direct transplanted neural progenitors towards a wound repair astroglial phenotype in mice. Nat Commun. 2022;13:5702 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 1c
Ponzetti M, Ucci A, Maurizi A, Giacchi L, Teti A, Rucci N. Lipocalin 2 Influences Bone and Muscle Phenotype in the MDX Mouse Model of Duchenne Muscular Dystrophy. Int J Mol Sci. 2022;23: pubmed publisher
  • western blot; mouse; loading ...; fig 4d
Helmrich N, Roderfeld M, Baier A, Windhorst A, Herebian D, Mayatepek E, et al. Pharmacologic Antagonization of Cannabinoid Receptor 1 Improves Cholestasis in Abcb4-/- Mice. Cell Mol Gastroenterol Hepatol. 2022;13:1041-1055 pubmed publisher
  • immunohistochemistry; mouse; fig 1c
Olson B, Zhu X, Norgard M, Diba P, Levasseur P, Buenafe A, et al. Chronic cerebral lipocalin 2 exposure elicits hippocampal neuronal dysfunction and cognitive impairment. Brain Behav Immun. 2021;97:102-118 pubmed publisher
  • western blot; mouse; loading ...; fig 2
Yoneshige A, Hagiyama M, Takashima Y, Ueno S, Inoue T, Kimura R, et al. Elevated Hydrostatic Pressure Causes Retinal Degeneration Through Upregulating Lipocalin-2. Front Cell Dev Biol. 2021;9:664327 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:100; loading ...; fig s10f
Zarb Y, Sridhar S, Nassiri S, Utz S, Schaffenrath J, Maheshwari U, et al. Microglia control small vessel calcification via TREM2. Sci Adv. 2021;7: pubmed publisher
Vlasschaert C, Robinson Cohen C, Chen J, Akwo E, Parker A, Silver S, et al. Clonal hematopoiesis of indeterminate potential is associated with acute kidney injury. Nat Med. 2024;30:810-817 pubmed publisher
Su H, Guo H, Qiu X, Lin T, Qin C, Celio G, et al. Lipocalin 2 regulates mitochondrial phospholipidome remodeling, dynamics, and function in brown adipose tissue in male mice. Nat Commun. 2023;14:6729 pubmed publisher
Beltran Lobo P, Hughes M, Troakes C, Croft C, Rupawala H, Jutzi D, et al. P2X7R influences tau aggregate burden in human tauopathies and shows distinct signalling in microglia and astrocytes. Brain Behav Immun. 2023;114:414-429 pubmed publisher
Marques E, Alves Teixeira M, Nguyen C, Terzi F, Gallazzini M. Lipocalin-2 induces mitochondrial dysfunction in renal tubular cells via mTOR pathway activation. Cell Rep. 2023;42:113032 pubmed publisher
Deng Z, Wang Y, Liu J, Zhang H, Zhou L, Zhao H, et al. WBP2 restrains the lysosomal degradation of GPX4 to inhibit ferroptosis in cisplatin-induced acute kidney injury. Redox Biol. 2023;65:102826 pubmed publisher
Patel S, Sparman N, Arneson D, Alvarsson A, Santos L, Duesman S, et al. Mammary duct luminal epithelium controls adipocyte thermogenic programme. Nature. 2023;620:192-199 pubmed publisher
Berquez M, Chen Z, Festa B, Krohn P, Keller S, Parolo S, et al. Lysosomal cystine export regulates mTORC1 signaling to guide kidney epithelial cell fate specialization. Nat Commun. 2023;14:3994 pubmed publisher
Pepino L, Malapert P, Saurin A, Moqrich A, Reynders A. Formalin-evoked pain triggers sex-specific behavior and spinal immune response. Sci Rep. 2023;13:9515 pubmed publisher
Vlasschaert C, Robinson Cohen C, Kestenbaum B, Silver S, Chen J, Akwo E, et al. Clonal Hematopoiesis of Indeterminate Potential is Associated with Acute Kidney Injury. medRxiv. 2023;: pubmed publisher
Tanaka S, Wakui H, Azushima K, Tsukamoto S, Yamaji T, Urate S, et al. Effects of a High-Protein Diet on Kidney Injury under Conditions of Non-CKD or CKD in Mice. Int J Mol Sci. 2023;24: pubmed publisher
Liu Y, Smith M, Wang Y, D AGOSTINO R, Ruiz J, Lycan T, et al. c-Met Mediated Cytokine Network Promotes Brain Metastasis of Breast Cancer by Remodeling Neutrophil Activities. Cancers (Basel). 2023;15: pubmed publisher
Wang D, Li X, Jiao D, Cai Y, Qian L, Shen Y, et al. LCN2 secreted by tissue-infiltrating neutrophils induces the ferroptosis and wasting of adipose and muscle tissues in lung cancer cachexia. J Hematol Oncol. 2023;16:30 pubmed publisher
Nagayama I, Takayanagi K, Hasegawa H, Maeshima A. Tubule-Derived Follistatin Is Increased in the Urine of Rats with Renal Ischemia and Reflects the Severity of Acute Tubular Damage. Cells. 2023;12: pubmed publisher
Mei T, Wu J, Wu K, Zhao M, Luo J, Liu X, et al. Lipocalin 2 induces visual impairment by promoting ferroptosis in retinal ischemia-reperfusion injury. Ann Transl Med. 2023;11:3 pubmed publisher
Greite R, St xf6 rmer J, Gueler F, Khalikov R, Haverich A, K xfc hn C, et al. Different Acute Kidney Injury Patterns after Renal Ischemia Reperfusion Injury and Extracorporeal Membrane Oxygenation in Mice. Int J Mol Sci. 2022;23: pubmed publisher
McCullough K, Akhter J, Taheri M, Traylor A, Zmijewska A, Verma V, et al. Functional consequence of myeloid ferritin heavy chain on acute and chronic effects of rhabdomyolysis-induced kidney injury. Front Med (Lausanne). 2022;9:894521 pubmed publisher
Zhang C, Guan Y, Zou J, Yang X, Bayliss G, Zhuang S. Histone methyltransferase MLL1 drives renal tubular cell apoptosis by p53-dependent repression of E-cadherin during cisplatin-induced acute kidney injury. Cell Death Dis. 2022;13:770 pubmed publisher
Sheldon J, Himmel L, Kunkle D, Monteith A, Maloney K, Skaar E. Lipocalin-2 is an essential component of the innate immune response to Acinetobacter baumannii infection. PLoS Pathog. 2022;18:e1010809 pubmed publisher
Gohar E, Almutlaq R, Fan C, Balkawade R, Butt M, Curtis L. Does G Protein-Coupled Estrogen Receptor 1 Contribute to Cisplatin-Induced Acute Kidney Injury in Male Mice?. Int J Mol Sci. 2022;23: pubmed publisher
Silva Oliveira Junior M, Schira Heinen J, Reiche L, Han S, de Amorim V, Lewen I, et al. Myelin repair is fostered by the corticosteroid medrysone specifically acting on astroglial subpopulations. EBioMedicine. 2022;83:104204 pubmed publisher
Shin H, Jin Z, An H, Park G, Lee J, Lee S, et al. Lipocalin-2 Deficiency Reduces Hepatic and Hippocampal Triggering Receptor Expressed on Myeloid Cells-2 Expressions in High-Fat Diet/Streptozotocin-Induced Diabetic Mice. Brain Sci. 2022;12: pubmed publisher
P xe9 rez Mart xed A, Ramakrishnan S, Li J, Dugourd A, Molenaar M, De La Motte L, et al. Reducing lipid bilayer stress by monounsaturated fatty acids protects renal proximal tubules in diabetes. elife. 2022;11: pubmed publisher
Xiang X, Tang X, Yu Y, Xie S, Liu L, Chen M, et al. Role of lipocalin-2 in surgery-induced cognitive decline in mice: a signal from neuron to microglia. J Neuroinflammation. 2022;19:92 pubmed publisher
Eckes T, Patyna S, Koch A, Oftring A, Gauer S, Oberm xfc ller N, et al. Sphingosine 1-Phosphate Receptor 5 (S1P5) Knockout Ameliorates Adenine-Induced Nephropathy. Int J Mol Sci. 2022;23: pubmed publisher
Wan T, Zhu W, Zhao Y, Zhang X, Ye R, Zuo M, et al. Astrocytic phagocytosis contributes to demyelination after focal cortical ischemia in mice. Nat Commun. 2022;13:1134 pubmed publisher
Shen T, Stauber J, Xu K, Jacunski A, Paragas N, Callahan M, et al. Snapshots of nascent RNA reveal cell- and stimulus-specific responses to acute kidney injury. JCI Insight. 2022;7: pubmed publisher
Krohn P, Rega L, Harvent M, Festa B, Taranta A, Luciani A, et al. Multisystem involvement, defective lysosomes and impaired autophagy in a novel rat model of nephropathic cystinosis. Hum Mol Genet. 2022;31:2262-2278 pubmed publisher
Sasaki K, Terker A, Tang J, Cao S, Arroyo J, Niu A, et al. Macrophage interferon regulatory factor 4 deletion ameliorates aristolochic acid nephropathy via reduced migration and increased apoptosis. JCI Insight. 2022;7: pubmed publisher
Majumder S, Pushpakumar S, Juin S, Jala V, Sen U. Toll-like receptor 4 mutation protects the kidney from Ang-II-induced hypertensive injury. Pharmacol Res. 2022;175:106030 pubmed publisher
Cheng Z, Akatsuka S, Li G, Mori K, Takahashi T, Toyokuni S. Ferroptosis resistance determines high susceptibility of murine A/J strain to iron-induced renal carcinogenesis. Cancer Sci. 2022;113:65-78 pubmed publisher
Yao F, Deng Y, Zhao Y, Mei Y, Zhang Y, Liu X, et al. A targetable LIFR-NF-κB-LCN2 axis controls liver tumorigenesis and vulnerability to ferroptosis. Nat Commun. 2021;12:7333 pubmed publisher
Ma N, Wei Z, Hu J, Gu W, Ci X. Farrerol Ameliorated Cisplatin-Induced Chronic Kidney Disease Through Mitophagy Induction via Nrf2/PINK1 Pathway. Front Pharmacol. 2021;12:768700 pubmed publisher
Talley S, Valiauga R, Anderson L, Cannon A, Choudhry M, Campbell E. DSS-induced inflammation in the colon drives a proinflammatory signature in the brain that is ameliorated by prophylactic treatment with the S100A9 inhibitor paquinimod. J Neuroinflammation. 2021;18:263 pubmed publisher
Ahn H, Lee G, Kim J, Park J, Kang S, Yoon S, et al. NLRP3 Triggers Attenuate Lipocalin-2 Expression Independent with Inflammasome Activation. Cells. 2021;10: pubmed publisher
Brooks J, Behrendt C, Ruhn K, Lee S, Raj P, Takahashi J, et al. The microbiota coordinates diurnal rhythms in innate immunity with the circadian clock. Cell. 2021;184:4154-4167.e12 pubmed publisher
Zhao S, Wang X, Zheng X, Liang X, Wang Z, Zhang J, et al. Iron deficiency exacerbates cisplatin- or rhabdomyolysis-induced acute kidney injury through promoting iron-catalyzed oxidative damage. Free Radic Biol Med. 2021;173:81-96 pubmed publisher
Olson B, Zhu X, Norgard M, Levasseur P, Butler J, BUENAFE A, et al. Lipocalin 2 mediates appetite suppression during pancreatic cancer cachexia. Nat Commun. 2021;12:2057 pubmed publisher
Heller G, Marshall M, Issa Y, Marshall J, Nguyen D, Rue E, et al. Waning efficacy in a long-term AAV-mediated gene therapy study in the murine model of Krabbe disease. Mol Ther. 2021;29:1883-1902 pubmed publisher
Staurenghi E, Cerrato V, Gamba P, Testa G, Giannelli S, Leoni V, et al. Oxysterols present in Alzheimer's disease brain induce synaptotoxicity by activating astrocytes: A major role for lipocalin-2. Redox Biol. 2021;39:101837 pubmed publisher
Merle N, Leon J, Poillerat V, Grunenwald A, Boudhabhay I, Knockaert S, et al. Circulating FH Protects Kidneys From Tubular Injury During Systemic Hemolysis. Front Immunol. 2020;11:1772 pubmed publisher
Borkham Kamphorst E, Haas U, Van De Leur E, Trevanich A, Weiskirchen R. Chronic Carbon Tetrachloride Applications Induced Hepatocyte Apoptosis in Lipocalin 2 Null Mice Through Endoplasmic Reticulum Stress and Unfolded Protein Response. Int J Mol Sci. 2020;21: pubmed publisher
Mondal A, Bose D, Saha P, Sarkar S, Seth R, Kimono D, et al. Lipocalin 2 induces neuroinflammation and blood-brain barrier dysfunction through liver-brain axis in murine model of nonalcoholic steatohepatitis. J Neuroinflammation. 2020;17:201 pubmed publisher
Xu C, Wang L, Zhang Y, Li W, Li J, Wang Y, et al. Tubule-Specific Mst1/2 Deficiency Induces CKD via YAP and Non-YAP Mechanisms. J Am Soc Nephrol. 2020;31:946-961 pubmed publisher
Liu Y, Li M, Yan Y, Wei W, Li B, Pan H, et al. ERα-dependent stimulation of LCN2 in uterine epithelium during mouse early pregnancy. Reproduction. 2020;159:493-501 pubmed publisher
Buhl E, Djudjaj S, Klinkhammer B, Ermert K, Puelles V, Lindenmeyer M, et al. Dysregulated mesenchymal PDGFR-β drives kidney fibrosis. EMBO Mol Med. 2020;12:e11021 pubmed publisher
Smith H, Freeman O, Butcher A, Holmqvist S, Humoud I, Schätzl T, et al. Astrocyte Unfolded Protein Response Induces a Specific Reactivity State that Causes Non-Cell-Autonomous Neuronal Degeneration. Neuron. 2020;: pubmed publisher
Chella Krishnan K, Sabir S, Shum M, Meng Y, Acin Perez R, Lang J, et al. Sex-specific metabolic functions of adipose Lipocalin-2. Mol Metab. 2019;30:30-47 pubmed publisher
Yammine L, Zablocki A, BARON W, Terzi F, Gallazzini M. Lipocalin-2 Regulates Epidermal Growth Factor Receptor Intracellular Trafficking. Cell Rep. 2019;29:2067-2077.e6 pubmed publisher
Xu Y, Zhu Y, Jadhav K, Li Y, Sun H, Yin L, et al. Lipocalin-2 Protects Against Diet-Induced Nonalcoholic Fatty Liver Disease by Targeting Hepatocytes. Hepatol Commun. 2019;3:763-775 pubmed publisher
Meade K, Sanchez F, Aguayo A, Nadales N, Hamalian S, Uhlendorf T, et al. Secretomes from metastatic breast cancer cells, enriched for a prognostically unfavorable LCN2 axis, induce anti-inflammatory MSC actions and a tumor-supportive premetastatic lung. Oncotarget. 2019;10:3027-3039 pubmed publisher
Noh K, Lee H, Choi T, Joo Y, Kim S, Kim H, et al. Negr1 controls adult hippocampal neurogenesis and affective behaviors. Mol Psychiatry. 2019;: pubmed publisher
Arif A, Jia J, Willard B, Li X, Fox P. Multisite Phosphorylation of S6K1 Directs a Kinase Phospho-code that Determines Substrate Selection. Mol Cell. 2019;73:446-457.e6 pubmed publisher
Yakulov T, Todkar A, Slanchev K, Wiegel J, Bona A, Groß M, et al. CXCL12 and MYC control energy metabolism to support adaptive responses after kidney injury. Nat Commun. 2018;9:3660 pubmed publisher
Langhorst H, Jüttner R, Groneberg D, Mohtashamdolatshahi A, Pelz L, Purfürst B, et al. The IgCAM CLMP regulates expression of Connexin43 and Connexin45 in intestinal and ureteral smooth muscle contraction in mice. Dis Model Mech. 2018;11: pubmed publisher
Ueno S, Yoneshige A, Koriyama Y, Hagiyama M, Shimomura Y, Ito A. Early Gene Expression Profile in Retinal Ganglion Cell Layer After Optic Nerve Crush in Mice. Invest Ophthalmol Vis Sci. 2018;59:370-380 pubmed publisher
Kim Y, Park S, Manson S, Molina C, Kidd K, Thiessen Philbrook H, et al. Elevated urinary CRELD2 is associated with endoplasmic reticulum stress-mediated kidney disease. JCI Insight. 2017;2: pubmed publisher
Al Rifai O, Chow J, LaCombe J, Julien C, Faubert D, Susan Resiga D, et al. Proprotein convertase furin regulates osteocalcin and bone endocrine function. J Clin Invest. 2017;127:4104-4117 pubmed publisher
Trudu M, Schaeffer C, Riba M, Ikehata M, Brambilla P, Messa P, et al. Early involvement of cellular stress and inflammatory signals in the pathogenesis of tubulointerstitial kidney disease due to UMOD mutations. Sci Rep. 2017;7:7383 pubmed publisher
Eskilsson A, Matsuwaki T, Shionoya K, Mirrasekhian E, Zajdel J, Schwaninger M, et al. Immune-Induced Fever Is Dependent on Local But Not Generalized Prostaglandin E2 Synthesis in the Brain. J Neurosci. 2017;37:5035-5044 pubmed publisher
Mosialou I, Shikhel S, Liu J, Maurizi A, Luo N, He Z, et al. MC4R-dependent suppression of appetite by bone-derived lipocalin 2. Nature. 2017;543:385-390 pubmed publisher
Björk Wilhelms D, Mirrasekhian E, Zajdel J, Kumar Singh A, Engblom D. Cyclooxygenase Isoform Exchange Blocks Brain-Mediated Inflammatory Symptoms. PLoS ONE. 2016;11:e0166153 pubmed publisher
Tan R, Chartoumpekis D, Rush B, Zhou D, Fu H, Kensler T, et al. Keap1 hypomorphism protects against ischemic and obstructive kidney disease. Sci Rep. 2016;6:36185 pubmed publisher
Jellbauer S, Perez Lopez A, Behnsen J, Gao N, Nguyen T, Murphy C, et al. Beneficial Effects of Sodium Phenylbutyrate Administration during Infection with Salmonella enterica Serovar Typhimurium. Infect Immun. 2016;84:2639-52 pubmed publisher
Deriu E, Boxx G, He X, Pan C, Benavidez S, Cen L, et al. Influenza Virus Affects Intestinal Microbiota and Secondary Salmonella Infection in the Gut through Type I Interferons. PLoS Pathog. 2016;12:e1005572 pubmed publisher
Song G, Jung M, Kim J, Park H, Rahman M, Zhang S, et al. A novel role for protein tyrosine phosphatase 1B as a positive regulator of neuroinflammation. J Neuroinflammation. 2016;13:86 pubmed publisher
product information
brand :
R&D Systems
master code :
AF1857
SKU :
AF1857
product name :
Mouse Lipocalin-2/NGAL Antibody
unit size :
100 ug (also 25 ug)
seo description :
The Mouse Lipocalin-2/NGAL Antibody from R&D Systems is a goat polyclonal antibody to Lipocalin-2/NGAL. This antibody reacts with human,mouse,transgenic mouse. The Mouse Lipocalin-2/NGAL Antibody has been validated for the following applications: Western Blot,Neutralization,Immunohistochemistry,Westen Blot,Bioassay,ELISA Development,IHC/IF,Immunoprecipitation,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunocytochemistry,Simple Western.
target :
Lipocalin-2/NGAL
category :
Primary Antibodies
buffer :
Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.
clonality :
Polyclonal
concentration :
LYOPH
conjugate :
Unconjugated
dilution :
Western Blot 0.25 ug/mL, Simple Western 5 ug/mL, Immunohistochemistry 3-15 ug/mL, Immunoprecipitation 25 ug/mL
host :
Goat
immunogen :
Mouse myeloma cell line NS0-derived recombinant mouse Lipocalin-2/NGAL, Gln21-Asn200, Accession # P11672
isotype :
IgG
purity :
Antigen Affinity-purified
species :
Human,Mouse,Transgenic Mouse
specificity :
Detects mouse Lipocalin-2/NGAL in direct ELISAs and Western blots.
gene symbol :
LCN2
top caption :
Detection of Mouse Lipocalin-2/NGAL antibody by Western Blot.
accessionNumbers :
P11672
applications :
Simple Western,Bioassay,ELISA Development,IHC/IF,Westen Blot,Immunohistochemistry,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Neutralization,Immunocytochemistry
USD :
499 USD
alt names :
24p3, 25 kDa alpha-2-microglobulin-related subunit of MMP-9, HNL, LCN2, lipocalin 2, lipocalin 2 (oncogene 24p3), Lipocalin2, Lipocalin-2, migration-stimulating factor inhibitor, MSFI, neutrophil gelatinase-associated lipocalin, NGAL, NGALlipocalin-2, Oncogene 24p3, p25, Siderocalin, Uterocalin
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.
more info or order :
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.