product summary
Loading...
company name :
R&D Systems
product type :
antibody
product name :
Human/Mouse Oct-3/4 Antibody
catalog :
AF1759
quantity :
100 ug (also 25 ug)
price :
499 USD
clonality :
polyclonal
host :
domestic goat
conjugate :
nonconjugated
reactivity :
human, mouse, rat, rhesus macaque
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, chromatin immunoprecipitation, immunohistochemistry - frozen section
more info or order :
citations: 47
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; mouse; 1:500; loading ...; fig 1g
O Shea T, Ao Y, Wang S, Wollenberg A, Kim J, Ramos Espinoza R, et al. Lesion environments direct transplanted neural progenitors towards a wound repair astroglial phenotype in mice. Nat Commun. 2022;13:5702 pubmed publisher
  • immunocytochemistry; human; 1:100; loading ...; fig s1c
Elhussieny A, Nogami K, Sakai Takemura F, Maruyama Y, Takemura N, Soliman W, et al. Mesenchymal stem cells derived from human induced pluripotent stem cells improve the engraftment of myogenic cells by secreting urokinase-type plasminogen activator receptor (uPAR). Stem Cell Res Ther. 2021;12:532 pubmed publisher
  • immunocytochemistry; human; 1:1000; loading ...; fig 1b
Yamamura Y, Furuichi K, Murakawa Y, Hirabayashi S, Yoshihara M, Sako K, et al. Identification of candidate PAX2-regulated genes implicated in human kidney development. Sci Rep. 2021;11:9123 pubmed publisher
  • immunocytochemistry; human; 1:400; fig s7
Grönroos P, Ilmarinen T, Skottman H. Directed Differentiation of Human Pluripotent Stem Cells towards Corneal Endothelial-Like Cells under Defined Conditions. Cells. 2021;10: pubmed publisher
  • immunocytochemistry; human; 1:500; fig 1c
Sarvestani S, SIGNS S, Hu B, Yeu Y, Feng H, Ni Y, et al. Induced organoids derived from patients with ulcerative colitis recapitulate colitic reactivity. Nat Commun. 2021;12:262 pubmed publisher
Viheri xe4 l xe4 T, Hongisto H, Saari L, Oksanen M, Ilmarinen T, V xe4 xe4 r xe4 m xe4 ki S, et al. Novel Human Induced Pluripotent Stem Cell-Based Model for Retinal Pigment Epithelial Cells to Reveal Possible Disease Mechanisms for Macular Degeneration in Pseudoxanthoma Elasticum. J Ophthalmol. 2024;2024:6939920 pubmed publisher
Raabe J, Wittig I, Laurette P, Stathopoulou K, Brand T, Schulze T, et al. Physioxia rewires mitochondrial complex composition to protect stem cell viability. Redox Biol. 2024;77:103352 pubmed publisher
Kojima R, Paslawski W, Lyu G, Arenas E, Zhang X, Svenningsson P. Secretome Analyses Identify FKBP4 as a GBA1-Associated Protein in CSF and iPS Cells from Parkinson's Disease Patients with GBA1 Mutations. Int J Mol Sci. 2024;25: pubmed publisher
Mendonca D, Cappuccio G, Sheppard J, Delacruz M, Bengtsson J, Carvalho C, et al. Generation of five induced pluripotent stem cell lines from patients with MECP2 Duplication Syndrome. Stem Cell Res. 2024;74:103292 pubmed publisher
Hatim O, Pavlinov I, Xu M, Linask K, Beers J, Liu C, et al. Generation of an Alagille syndrome (ALGS) patient-derived induced pluripotent stem cell line (TRNDi032-A) carrying a heterozygous mutation (p.Cys682Leufs*7) in the JAG1 gene. Stem Cell Res. 2023;73:103231 pubmed publisher
Su Y, Gu S, Liu Y, Cheng Y, Wan Q, Sang X, et al. Partial cellular reprogramming stably restores the stemness of senescent epidermal stem cells. Eur Rev Med Pharmacol Sci. 2023;27:5397-5409 pubmed publisher
Wang Y, Peng J, Song C, Yang Y, Qin T. Zinc finger and SCAN domain-containing 18 suppresses the proliferation, self-renewal, and drug resistance of glioblastoma cells. Heliyon. 2023;9:e17000 pubmed publisher
Li J, Zhu Q, Cao J, Liu Y, Lu Y, Sun Y, et al. Cynomolgus monkey embryo model captures gastrulation and early pregnancy. Cell Stem Cell. 2023;30:362-377.e7 pubmed publisher
Almad A, Garcia L, Takanohashi A, Gagne A, Yang W, Ann McGuire J, et al. Generation of three induced Pluripotent Stem Cell lines from individuals with Hypomyelination with Atrophy of Basal Ganglia and Cerebellum caused by a c.745G>A (p.D249N) autosomal dominant mutation in TUBB4A. Stem Cell Res. 2023;69:103083 pubmed publisher
Shigyo M, Kobayashi Y, Platoshyn O, Marsala S, Kato T, Takamura N, et al. Derivation of Sendai-Virus-Reprogrammed Human iPSCs-Neuronal Precursors: In Vitro and In Vivo Post-grafting Safety Characterization. Cell Transplant. 2023;32:9636897231163232 pubmed publisher
Aguirre V xe1 zquez A, Castorena Torres F, Silva Ram xed rez B, Pe xf1 uelas Urquides K, Camacho Moll M, Salazar Olivo L, et al. Cell-type dependent regulation of pluripotency and chromatin remodeling genes by hydralazine. Stem Cell Res Ther. 2023;14:42 pubmed publisher
Luo Q, Pui H, Chen J, Yu L, Jannig P, Pei Y, et al. Epiblast-like stem cells established by Wnt/β-catenin signaling manifest distinct features of formative pluripotency and germline competence. Cell Rep. 2023;42:112021 pubmed publisher
Liu X, Ye Y, Zhu L, Xiao X, Zhou B, Gu Y, et al. Niche stiffness sustains cancer stemness via TAZ and NANOG phase separation. Nat Commun. 2023;14:238 pubmed publisher
Yelagandula R, Stecher K, Novatchkova M, Michetti L, Michlits G, Wang J, et al. ZFP462 safeguards neural lineage specification by targeting G9A/GLP-mediated heterochromatin to silence enhancers. Nat Cell Biol. 2023;25:42-55 pubmed publisher
Ruan D, Ye Z, Yuan S, Li Z, Zhang W, Ong C, et al. Human early syncytiotrophoblasts are highly susceptible to SARS-CoV-2 infection. Cell Rep Med. 2022;3:100849 pubmed publisher
Nakashima Y, Miyagi Shiohira C, Saitoh I, Watanabe M, Matsushita M, Tsukahara M, et al. Induced hepatic stem cells are suitable for human hepatocyte production. iScience. 2022;25:105052 pubmed publisher
Lotila J, Hyv xe4 rinen T, Skottman H, Airas L, Narkilahti S, Hagman S. Establishment of a human induced pluripotent stem cell line (TAUi008-A) derived from a multiple sclerosis patient. Stem Cell Res. 2022;63:102865 pubmed publisher
Francescangeli F, De Angelis M, Rossi R, Sette G, Eramo A, Boe A, et al. CRIPTO Is a Marker of Chemotherapy-Induced Stem Cell Expansion in Non-Small Cell Lung Cancer. Front Oncol. 2022;12:830873 pubmed publisher
Simunovic M, Siggia E, Brivanlou A. In vitro attachment and symmetry breaking of a human embryo model assembled from primed embryonic stem cells. Cell Stem Cell. 2022;29:962-972.e4 pubmed publisher
Xiong L, Tolen E, Choi J, Velychko S, Caizzi L, Velychko T, et al. Oct4 differentially regulates chromatin opening and enhancer transcription in pluripotent stem cells. elife. 2022;11: pubmed publisher
Sesé B, Íñiguez Muñoz S, Ensenyat Mendez M, Llinàs Arias P, Ramis G, Orozco J, et al. Glioblastoma Embryonic-like Stem Cells Exhibit Immune-Evasive Phenotype. Cancers (Basel). 2022;14: pubmed publisher
Kanno S, Okubo Y, Kageyama T, Yan L, Kitajima S, Fukuda J. Establishment of a developmental toxicity assay based on human iPSC reporter to detect FGF signal disruption. iScience. 2022;25:103770 pubmed publisher
Pagliaroli L, Porazzi P, Curtis A, Scopa C, Mikkers H, Freund C, et al. Inability to switch from ARID1A-BAF to ARID1B-BAF impairs exit from pluripotency and commitment towards neural crest formation in ARID1B-related neurodevelopmental disorders. Nat Commun. 2021;12:6469 pubmed publisher
Ma L, Schmidt M, Morrow E. Human iPSC lines from a Christianson syndrome patient with NHE6 W523X mutation, a biologically-related control, and CRISPR/Cas9 gene-corrected isogenic controls. Stem Cell Res. 2021;54:102435 pubmed publisher
Lee M, Stebbins M, Jiao H, Huang H, Leiferman E, Walczak B, et al. Comparative evaluation of isogenic mesodermal and ectomesodermal chondrocytes from human iPSCs for cartilage regeneration. Sci Adv. 2021;7: pubmed publisher
Mor M, Harvey A, Familari M, St Clair Glover M, Viventi S, de Iongh R, et al. Neural differentiation medium for human pluripotent stem cells to model physiological glucose levels in human brain. Brain Res Bull. 2021;173:141-149 pubmed publisher
Liu N, Maresca M, van den Brand T, Braccioli L, Schijns M, Teunissen H, et al. WAPL maintains a cohesin loading cycle to preserve cell-type-specific distal gene regulation. Nat Genet. 2021;53:100-109 pubmed publisher
Trevellin E, Pirozzolo G, Fassan M, Vettor R. Prognostic value of stem cell markers in esophageal and esophagogastric junction cancer: a meta-analysis. J Cancer. 2020;11:4240-4249 pubmed publisher
Hazelbaker D, Beccard A, Angelini G, Mazzucato P, Messana A, Lam D, et al. A multiplexed gRNA piggyBac transposon system facilitates efficient induction of CRISPRi and CRISPRa in human pluripotent stem cells. Sci Rep. 2020;10:635 pubmed publisher
Hyvärinen T, Hyysalo A, Kapucu F, Aarnos L, Vinogradov A, Eglen S, et al. Functional characterization of human pluripotent stem cell-derived cortical networks differentiated on laminin-521 substrate: comparison to rat cortical cultures. Sci Rep. 2019;9:17125 pubmed publisher
Vlahova F, Hawkins K, Ranzoni A, Hau K, Sagar R, Coppi P, et al. Human mid-trimester amniotic fluid (stem) cells lack expression of the pluripotency marker OCT4A. Sci Rep. 2019;9:8126 pubmed publisher
Sherman Samis M, Onallah H, Holth A, Reich R, Davidson B. SOX2 and SOX9 are markers of clinically aggressive disease in metastatic high-grade serous carcinoma. Gynecol Oncol. 2019;153:651-660 pubmed publisher
Ichisima J, Suzuki N, Samata B, Awaya T, Takahashi J, Hagiwara M, et al. Verification and rectification of cell type-specific splicing of a Seckel syndrome-associated ATR mutation using iPS cell model. J Hum Genet. 2019;64:445-458 pubmed publisher
Cao B, Duan M, Xing Y, Liu C, Yang F, Li Y, et al. O-GlcNAc transferase activates stem-like cell potential in hepatocarcinoma through O-GlcNAcylation of eukaryotic initiation factor 4E. J Cell Mol Med. 2019;23:2384-2398 pubmed publisher
Zhang W, Chronis C, Chen X, Zhang H, Spalinskas R, Pardo M, et al. The BAF and PRC2 Complex Subunits Dpf2 and Eed Antagonistically Converge on Tbx3 to Control ESC Differentiation. Cell Stem Cell. 2019;24:138-152.e8 pubmed publisher
Kaur S, Abu Shahba A, Paananen R, Hongisto H, Hiidenmaa H, Skottman H, et al. Small non-coding RNA landscape of extracellular vesicles from human stem cells. Sci Rep. 2018;8:15503 pubmed publisher
Buskin A, Zhu L, Chichagova V, Basu B, Mozaffari Jovin S, Dolan D, et al. Disrupted alternative splicing for genes implicated in splicing and ciliogenesis causes PRPF31 retinitis pigmentosa. Nat Commun. 2018;9:4234 pubmed publisher
Gao X, Yourick J, Sprando R. Generation of nine induced pluripotent stem cell lines as an ethnic diversity panel. Stem Cell Res. 2018;31:193-196 pubmed publisher
Alsayegh K, Matsuura K, Sekine H, Shimizu T. Dinaciclib potently suppresses MCL-1 and selectively induces the cell death in human iPS cells without affecting the viability of cardiac tissue. Sci Rep. 2017;7:45577 pubmed publisher
Palmero E, Palmero S, Murrell W. Brain tissue banking for stem cells for our future. Sci Rep. 2016;6:39394 pubmed publisher
Bara A, Messana A, Herring A, Hazelbaker D, Eggan K, Barrett L. Generation of a TLE3 heterozygous knockout human embryonic stem cell line using CRISPR-Cas9. Stem Cell Res. 2016;17:441-443 pubmed publisher
Herring A, Messana A, Bara A, Hazelbaker D, Eggan K, Barrett L. Generation of a TLE1 homozygous knockout human embryonic stem cell line using CRISPR-Cas9. Stem Cell Res. 2016;17:430-432 pubmed publisher
product information
brand :
R&D Systems
master code :
AF1759
SKU :
AF1759
product name :
Human/Mouse Oct-3/4 Antibody
unit size :
100 ug (also 25 ug)
seo description :
The Human/Mouse Oct-3/4 Antibody from R&D Systems is a goat polyclonal antibody to Oct-3/4. This antibody reacts with human,mouse,primate - macaca fascicularis (crab-eating monkey or cynomolgus macaque),primate - macaca mulatta (rhesus macaque),rabbit,rat. The Human/Mouse Oct-3/4 Antibody has been validated for the following applications: Bioassay,Immunocytochemistry,Co-Immunoprecipitation,Differentiation,Flow Cytometry,Western Blot,Immunohistochemistry,Immunohistochemistry-Frozen,Chromatin Immunoprecipitation (ChIP),Immunoprecipitation,Simple Western.
target :
Oct-3/4
category :
Primary Antibodies
buffer :
Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.
clonality :
Polyclonal
concentration :
LYOPH
conjugate :
Unconjugated
dilution :
Western Blot 0.5 ug/mL, Simple Western 20 ug/mL, Immunoprecipitation 3 ug/10^6 cells, Chromatin Immunoprecipitation (ChIP) 5 ug/10^6 cells, Immunocytochemistry 5-15 ug/mL
host :
Goat
immunogen :
E. coli -derived recombinant human Oct-3/4, Met1-Asn265 (Met262Leu), Accession # Q01860
isotype :
IgG
purity :
Antigen Affinity-purified
species :
Human,Mouse,Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque),Primate - Macaca mulatta (Rhesus Macaque),Rabbit,Rat
specificity :
Detects human Oct-3/4 in direct ELISAs and Western blots.
gene symbol :
POU5F1
top caption :
Detection of Human and Mouse Oct-3/4 antibody by Western Blot.
accessionNumbers :
Q01860
applications :
Immunoprecipitation,Simple Western,Co-Immunoprecipitation,Differentiation,Flow Cytometry,Immunohistochemistry,Western Blot,Immunohistochemistry-Frozen,Chromatin Immunoprecipitation (ChIP),Immunocytochemistry
USD :
499 USD
alt names :
3-Oct, OCT3;Oct-3;OCT4;Oct-4;OCT4;Oct3/4;OTF3;OTF-3;OTF4;POU domain protein;POU domain, class 5, transcription factor 1;POU5F1, Oct4, Otf3g, Otf4, Pou5f1
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.
more info or order :
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.