product summary
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company name :
R&D Systems
product type :
antibody
product name :
Human Podocalyxin Antibody
catalog :
AF1658
quantity :
100 ug (also 25 ug)
price :
499 USD
clonality :
polyclonal
host :
domestic goat
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
R&D Systems product webpage
citations: 30
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; human; 1:1000; loading ...; fig 2a
Yamamura Y, Furuichi K, Murakawa Y, Hirabayashi S, Yoshihara M, Sako K, et al. Identification of candidate PAX2-regulated genes implicated in human kidney development. Sci Rep. 2021;11:9123 pubmed publisher
Liu M, Zhang C, Gong X, Zhang T, Lian M, Chew E, et al. Kidney organoid models reveal cilium-autophagy metabolic axis as a therapeutic target for PKD both in vitro and in vivo. Cell Stem Cell. 2024;31:52-70.e8 pubmed publisher
Overeem A, Chang Y, Moustakas I, Roelse C, Hillenius S, Helm T, et al. Efficient and scalable generation of primordial germ cells in 2D culture using basement membrane extract overlay. Cell Rep Methods. 2023;3:100488 pubmed publisher
Chen Y, Chauhan S, Gong C, Dayton H, Xu C, De La Cruz E, et al. Scalable projected Light Sheet Microscopy for high-resolution imaging of living and cleared samples. bioRxiv. 2023;: pubmed publisher
Cui S, Fang X, Lee H, Shin Y, Koh E, Chung S, et al. Modeling of Fabry disease nephropathy using patient derived human induced pluripotent stem cells and kidney organoid system. J Transl Med. 2023;21:138 pubmed publisher
Hiratsuka K, Miyoshi T, Kroll K, Gupta N, Valerius M, Ferrante T, et al. Organoid-on-a-chip model of human ARPKD reveals mechanosensing pathomechanisms for drug discovery. Sci Adv. 2022;8:eabq0866 pubmed publisher
Amo L, D xed ez Garc xed a J, Tamayo Orbegozo E, Maruri N, Larrucea S. Podocalyxin Expressed in Antigen Presenting Cells Promotes Interaction With T Cells and Alters Centrosome Translocation to the Contact Site. Front Immunol. 2022;13:835527 pubmed publisher
Nystrom S, Li G, Datta S, Soldano K, Silas D, Weins A, et al. JAK inhibitor blocks COVID-19 cytokine-induced JAK/STAT/APOL1 signaling in glomerular cells and podocytopathy in human kidney organoids. JCI Insight. 2022;7: pubmed publisher
Gupta N, Matsumoto T, Hiratsuka K, Garcia Saiz E, Galichon P, Miyoshi T, et al. Modeling injury and repair in kidney organoids reveals that homologous recombination governs tubular intrinsic repair. Sci Transl Med. 2022;14:eabj4772 pubmed publisher
Winkler E, Kim C, Ross J, Garcia J, Gil E, Oh I, et al. A single-cell atlas of the normal and malformed human brain vasculature. Science. 2022;375:eabi7377 pubmed publisher
Helms L, Marchianò S, Stanaway I, Hsiang T, Juliar B, Saini S, et al. Cross-validation of SARS-CoV-2 responses in kidney organoids and clinical populations. JCI Insight. 2021;6: pubmed publisher
Hernandez J, Wang X, Vazquez Segoviano M, Lopez Marfil M, Sobral Reyes M, Moran Horowich A, et al. A tissue-bioengineering strategy for modeling rare human kidney diseases in vivo. Nat Commun. 2021;12:6496 pubmed publisher
Gupta A, Ivancic D, Naved B, Wertheim J, Oxburgh L. An efficient method to generate kidney organoids at the air-liquid interface. J Biol Methods. 2021;8:e150 pubmed publisher
Paulson D, Harms R, Ward C, Latterell M, Pazour G, Fink D. Loss of Primary Cilia Protein IFT20 Dysregulates Lymphatic Vessel Patterning in Development and Inflammation. Front Cell Dev Biol. 2021;9:672625 pubmed publisher
Chen Y, Tristan C, Chen L, Jovanovic V, Malley C, Chu P, et al. A versatile polypharmacology platform promotes cytoprotection and viability of human pluripotent and differentiated cells. Nat Methods. 2021;18:528-541 pubmed publisher
Ettou S, Jung Y, Miyoshi T, Jain D, Hiratsuka K, Schumacher V, et al. Epigenetic transcriptional reprogramming by WT1 mediates a repair response during podocyte injury. Sci Adv. 2020;6:eabb5460 pubmed publisher
Kumar Gupta A, Sarkar P, Wertheim J, Pan X, Carroll T, Oxburgh L. Asynchronous mixing of kidney progenitor cells potentiates nephrogenesis in organoids. Commun Biol. 2020;3:231 pubmed publisher
Yu A, Zhao L, Eaton K, Ho S, Chen J, Poe S, et al. Gut Microbiota Modulate CD8 T Cell Responses to Influence Colitis-Associated Tumorigenesis. Cell Rep. 2020;31:107471 pubmed publisher
Santelli A, Sun I, Eirin A, Abumoawad A, Woollard J, Lerman A, et al. Senescent Kidney Cells in Hypertensive Patients Release Urinary Extracellular Vesicles. J Am Heart Assoc. 2019;8:e012584 pubmed publisher
Low J, Li P, Chew E, Zhou B, Suzuki K, Zhang T, et al. Generation of Human PSC-Derived Kidney Organoids with Patterned Nephron Segments and a De Novo Vascular Network. Cell Stem Cell. 2019;25:373-387.e9 pubmed publisher
Hochane M, van den Berg P, Fan X, Bérenger Currias N, Adegeest E, Bialecka M, et al. Single-cell transcriptomics reveals gene expression dynamics of human fetal kidney development. PLoS Biol. 2019;17:e3000152 pubmed publisher
Harder J, Menon R, Otto E, Zhou J, Eddy S, Wys N, et al. Organoid single cell profiling identifies a transcriptional signature of glomerular disease. JCI Insight. 2019;4: pubmed publisher
Bajaj P, Rodrigues A, Steppan C, Engle S, Mathialagan S, Schroeter T. Human Pluripotent Stem Cell-Derived Kidney Model for Nephrotoxicity Studies. Drug Metab Dispos. 2018;46:1703-1711 pubmed publisher
Itai S, Ohishi T, Kaneko M, Yamada S, Abe S, Nakamura T, et al. Anti-podocalyxin antibody exerts antitumor effects via antibody-dependent cellular cytotoxicity in mouse xenograft models of oral squamous cell carcinoma. Oncotarget. 2018;9:22480-22497 pubmed publisher
Saito S, Hiemori K, Kiyoi K, Tateno H. Glycome analysis of extracellular vesicles derived from human induced pluripotent stem cells using lectin microarray. Sci Rep. 2018;8:3997 pubmed publisher
Müller Deile J, Schroder P, Beverly Staggs L, Hiss R, Fiedler J, Nyström J, et al. Overexpression of preeclampsia induced microRNA-26a-5p leads to proteinuria in zebrafish. Sci Rep. 2018;8:3621 pubmed publisher
Kang H, Lee M, Lee K, Hughes M, Kwon B, Lee S, et al. Loss of podocalyxin causes a novel syndromic type of congenital nephrotic syndrome. Exp Mol Med. 2017;49:e414 pubmed publisher
Volk Draper L, Hall K, Wilber A, Ran S. Lymphatic endothelial progenitors originate from plastic myeloid cells activated by toll-like receptor-4. PLoS ONE. 2017;12:e0179257 pubmed publisher
Morizane R, Bonventre J. Generation of nephron progenitor cells and kidney organoids from human pluripotent stem cells. Nat Protoc. 2017;12:195-207 pubmed publisher
Ziegler N, Awwad K, Fisslthaler B, Reis M, Devraj K, Corada M, et al. ?-Catenin Is Required for Endothelial Cyp1b1 Regulation Influencing Metabolic Barrier Function. J Neurosci. 2016;36:8921-35 pubmed publisher
product information
brand :
R&D Systems
master code :
AF1658
SKU :
AF1658
product name :
Human Podocalyxin Antibody
unit size :
100 ug (also 25 ug)
seo description :
The Human Podocalyxin Antibody from R&D Systems is a goat polyclonal antibody to Podocalyxin Like. This antibody reacts with human,mouse. The Human Podocalyxin Antibody has been validated for the following applications: Immunocytochemistry,Flow Cytometry,Western Blot,Immunohistochemistry,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,CyTOF-ready,Knockout Validated.
target :
Podocalyxin
category :
Primary Antibodies
buffer :
Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.
clonality :
Polyclonal
concentration :
LYOPH
conjugate :
Unconjugated
dilution :
Western Blot 1 ug/mL, Flow Cytometry 0.25 ug/10^6 cells, Immunohistochemistry 5-15 ug/mL, CyTOF-ready, Knockout Validated
host :
Goat
immunogen :
Mouse myeloma cell line NS0-derived recombinant human Podocalyxin, Ser23-Arg427, Accession # AAB61574
isotype :
IgG
purity :
Antigen Affinity-purified
species :
Human,Mouse
specificity :
Detects human Podocalyxin in direct ELISAs and Western blots.
gene symbol :
PODXL
Antibody validation :
Knockout/Knockdown
top caption :
Detection of Human Podocalyxin antibody by Western Blot.
accessionNumbers :
AAB61574
applications :
CyTOF-ready,Knockout Validated,Flow Cytometry,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunocytochemistry
USD :
499 USD
alt names :
GCTM, Gp200MGC138240, PCLP, PCLP1, PCLP-1, PCLPGCTM-2 antigen, PCpodocalyxin, POD XL, podocalyxin-like, Podocalyxin-like protein 1, PODXL
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.
more info or order :
R&D Systems product webpage
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.