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company name :
R&D Systems
product type :
antibody
product name :
Mouse SOST/Sclerostin Antibody
catalog :
AF1589
quantity :
100 ug (also 25 ug)
price :
499 USD
clonality :
polyclonal
host :
domestic goat
conjugate :
nonconjugated
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
citations: 68
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - paraffin section; mouse; 1:400; loading ...; fig 4d
Zhao D, Riquelme M, Guda T, Tu C, Xu H, Gu S, et al. Connexin hemichannels with prostaglandin release in anabolic function of bone to mechanical loading. elife. 2022;11: pubmed publisher
  • western blot; mouse; loading ...; fig 4b
Kim S, Henneicke H, Cavanagh L, Macfarlane E, Thai L, Foong D, et al. Osteoblastic glucocorticoid signaling exacerbates high-fat-diet- induced bone loss and obesity. Bone Res. 2021;9:40 pubmed publisher
  • immunohistochemistry; mouse; 1:100; loading ...
Teufel S, Köckemann P, Fabritius C, Wolff L, Bertrand J, Pap T, et al. Loss of the WNT9a ligand aggravates the rheumatoid arthritis-like symptoms in hTNF transgenic mice. Cell Death Dis. 2021;12:494 pubmed publisher
Turkkahraman H, Flanagan S, Zhu T, Akel N, Marino S, Ortega Gonzalez D, et al. Sclerostin antibody corrects periodontal disease in type 2 diabetic mice. JCI Insight. 2024;9: pubmed publisher
Takami K, Okamoto K, Etani Y, Hirao M, Miyama A, Okamura G, et al. Anti-NF-κB peptide derived from nuclear acidic protein attenuates ovariectomy-induced osteoporosis in mice. JCI Insight. 2023;8: pubmed publisher
Qin L, Chen Z, Yang D, He T, Xu Z, Zhang P, et al. Osteocyte β3 integrin promotes bone mass accrual and force-induced bone formation in mice. J Orthop Translat. 2023;40:58-71 pubmed publisher
Aobulikasimu A, Liu T, Piao J, Sato S, Ochi H, Okawa A, et al. SIRT6-PAI-1 axis is a promising therapeutic target in aging-related bone metabolic disruption. Sci Rep. 2023;13:7991 pubmed publisher
Guo Q, Chen N, Qian C, Qi C, Noller K, Wan M, et al. Sympathetic Innervation Regulates Osteocyte-Mediated Cortical Bone Resorption during Lactation. Adv Sci (Weinh). 2023;10:e2207602 pubmed publisher
Lamand xe9 S, Ng E, Cameron T, Kung L, Sampurno L, Rowley L, et al. Modeling human skeletal development using human pluripotent stem cells. Proc Natl Acad Sci U S A. 2023;120:e2211510120 pubmed publisher
Madel M, Halper J, Ib xe1 xf1 ez L, Claire L, Rouleau M, Boutin A, et al. Specific targeting of inflammatory osteoclastogenesis by the probiotic yeast S. boulardii CNCM I-745 reduces bone loss in osteoporosis. elife. 2023;12: pubmed publisher
Hiraga T, Horibe K, Koide M, Yamashita T, Kobayashi Y. Sclerostin blockade promotes bone metastases of Wnt-responsive breast cancer cells. Cancer Sci. 2023;114:2460-2470 pubmed publisher
Gao X, Sun X, Cheng H, Ruzbarsky J, Mullen M, Huard M, et al. MRL/MpJ Mice Resist to Age-Related and Long-Term Ovariectomy-Induced Bone Loss: Implications for Bone Regeneration and Repair. Int J Mol Sci. 2023;24: pubmed publisher
Kurgan N, Baranowski B, Stoikos J, MacNeil A, Fajardo V, Macpherson R, et al. Characterization of sclerostin's response within white adipose tissue to an obesogenic diet at rest and in response to acute exercise in male mice. Front Physiol. 2022;13:1061715 pubmed publisher
Yoshimoto T, Kittaka M, Doan A, Urata R, Prideaux M, Rojas R, et al. Osteocytes directly regulate osteolysis via MYD88 signaling in bacterial bone infection. Nat Commun. 2022;13:6648 pubmed publisher
Jeffery E, Mann T, Pool J, Zhao Z, Morrison S. Bone marrow and periosteal skeletal stem/progenitor cells make distinct contributions to bone maintenance and repair. Cell Stem Cell. 2022;29:1547-1561.e6 pubmed publisher
Jeon H, Kang J, Li J, KIM D, Yuan G, Almer N, et al. The Effect of IFT80 Deficiency in Osteocytes on Orthodontic Loading-Induced and Physiologic Bone Remodeling: In Vivo Study. Life (Basel). 2022;12: pubmed publisher
Zhao D, Hua R, Riquelme M, Cheng H, Guda T, Xu H, et al. Osteocytes regulate bone anabolic response to mechanical loading in male mice via activation of integrin α5. Bone Res. 2022;10:49 pubmed publisher
Wei T, Shan Z, Wen X, Zhao N, Shen G. Dynamic alternations of RANKL/OPG ratio expressed by cementocytes in response to orthodontic‑induced external apical root resorption in a rat model. Mol Med Rep. 2022;26: pubmed publisher
Jiang Y, Wang Z, Liu X, Wan M, Liu Y, Jiao B, et al. The Protective Effects of Osteocyte-Derived Extracellular Vesicles Against Alzheimer's Disease Diminished with Aging. Adv Sci (Weinh). 2022;9:e2105316 pubmed publisher
Moriishi T, Ito T, Fukuyama R, Qin X, Komori H, Kaneko H, et al. Sp7 Transgenic Mice with a Markedly Impaired Lacunocanalicular Network Induced Sost and Reduced Bone Mass by Unloading. Int J Mol Sci. 2022;23: pubmed publisher
Wang Z, Luo Z, Li F, Cao J, Rao S, Liu Y, et al. Aged bone matrix-derived extracellular vesicles as a messenger for calcification paradox. Nat Commun. 2022;13:1453 pubmed publisher
Gadomski S, Fielding C, Garc xed a Garc xed a A, Korn C, Kapeni C, Ashraf S, et al. A cholinergic neuroskeletal interface promotes bone formation during postnatal growth and exercise. Cell Stem Cell. 2022;29:528-544.e9 pubmed publisher
Kim J, Yang Y, Xie J, Lee O, Kim J, Hong J, et al. Regulation of sclerostin by the SIRT1 stabilization pathway in osteocytes. Cell Death Differ. 2022;: pubmed publisher
Lawson L, Brodt M, Migotsky N, Chermside Scabbo C, Palaniappan R, Silva M. Osteoblast-Specific Wnt Secretion Is Required for Skeletal Homeostasis and Loading-Induced Bone Formation in Adult Mice. J Bone Miner Res. 2022;37:108-120 pubmed publisher
Wehrle E, Paul G, Tourolle N xe9 Betts D, Kuhn G, M xfc ller R. Individualized cyclic mechanical loading improves callus properties during the remodelling phase of fracture healing in mice as assessed from time-lapsed in vivo imaging. Sci Rep. 2021;11:23037 pubmed publisher
Dixit M, Duran Ortiz S, Yildirim G, Poudel S, Louis L, Bartke A, et al. Induction of somatopause in adult mice compromises bone morphology and exacerbates bone loss during aging. Aging Cell. 2021;20:e13505 pubmed publisher
Jackson E, Lara Castillo N, Akhter M, Dallas M, Scott J, Ganesh T, et al. Osteocyte Wnt/β-catenin pathway activation upon mechanical loading is altered in ovariectomized mice. Bone Rep. 2021;15:101129 pubmed publisher
Gould N, Williams K, Joca H, Torre O, Lyons J, Leser J, et al. Disparate bone anabolic cues activate bone formation by regulating the rapid lysosomal degradation of sclerostin protein. elife. 2021;10: pubmed publisher
Baroi S, Czernik P, Chougule A, Griffin P, Lecka Czernik B. PPARG in osteocytes controls sclerostin expression, bone mass, marrow adiposity and mediates TZD-induced bone loss. Bone. 2021;147:115913 pubmed publisher
Wang Z, Weng Y, Ishihara Y, Odagaki N, Ei Hsu Hlaing E, Izawa T, et al. Loading history changes the morphology and compressive force-induced expression of receptor activator of nuclear factor kappa B ligand/osteoprotegerin in MLO-Y4 osteocytes. Peerj. 2020;8:e10244 pubmed publisher
Bailey K, NGUYEN J, Yee C, Dole N, Dang A, Alliston T. Mechanosensitive Control of Articular Cartilage and Subchondral Bone Homeostasis in Mice Requires Osteocytic Transforming Growth Factor β Signaling. Arthritis Rheumatol. 2021;73:414-425 pubmed publisher
Koide M, Yamashita T, Murakami K, Uehara S, Nakamura K, Nakamura M, et al. Sclerostin expression in trabecular bone is downregulated by osteoclasts. Sci Rep. 2020;10:13751 pubmed publisher
Prideaux M, Kitase Y, Kimble M, O Connell T, Bonewald L. Taurine, an osteocyte metabolite, protects against oxidative stress-induced cell death and decreases inhibitors of the Wnt/β-catenin signaling pathway. Bone. 2020;137:115374 pubmed publisher
Williams K, Leser J, Gould N, Joca H, Lyons J, Khairallah R, et al. TRPV4 calcium influx controls sclerostin protein loss independent of purinergic calcium oscillations. Bone. 2020;136:115356 pubmed publisher
Song J, Pineault K, Dones J, Raines R, Wellik D. Hox genes maintain critical roles in the adult skeleton. Proc Natl Acad Sci U S A. 2020;117:7296-7304 pubmed publisher
Li J, Xue J, Jing Y, Wang M, Shu R, Xu H, et al. SOST Deficiency Aggravates Osteoarthritis in Mice by Promoting Sclerosis of Subchondral Bone. Biomed Res Int. 2019;2019:7623562 pubmed publisher
Wehrle E, Tourolle Né Betts D, Kuhn G, Scheuren A, Hofmann S, Muller R. Evaluation of longitudinal time-lapsed in vivo micro-CT for monitoring fracture healing in mouse femur defect models. Sci Rep. 2019;9:17445 pubmed publisher
Pacicca D, Brown T, Watkins D, Kover K, Yan Y, Prideaux M, et al. Elevated glucose acts directly on osteocytes to increase sclerostin expression in diabetes. Sci Rep. 2019;9:17353 pubmed publisher
Metzger C, Narayanan S, Elizondo J, Carter A, Zawieja D, Hogan H, et al. DSS-induced colitis produces inflammation-induced bone loss while irisin treatment mitigates the inflammatory state in both gut and bone. Sci Rep. 2019;9:15144 pubmed publisher
Staines K, Ikpegbu E, Törnqvist A, Dillon S, Javaheri B, Amin A, et al. Conditional deletion of E11/podoplanin in bone protects against load-induced osteoarthritis. BMC Musculoskelet Disord. 2019;20:344 pubmed publisher
Ohori F, Kitaura H, Marahleh A, Kishikawa A, Ogawa S, Qi J, et al. Effect of TNF-α-Induced Sclerostin on Osteocytes during Orthodontic Tooth Movement. J Immunol Res. 2019;2019:9716758 pubmed publisher
Pineault K, Song J, Kozloff K, Lucas D, Wellik D. Hox11 expressing regional skeletal stem cells are progenitors for osteoblasts, chondrocytes and adipocytes throughout life. Nat Commun. 2019;10:3168 pubmed publisher
Nguyen Yamamoto L, Tanaka K, St Arnaud R, Goltzman D. Vitamin D-regulated osteocytic sclerostin and BMP2 modulate uremic extraskeletal calcification. JCI Insight. 2019;4: pubmed publisher
Lotinun S, Ishihara Y, Nagano K, Kiviranta R, Carpentier V, Neff L, et al. Cathepsin K-deficient osteocytes prevent lactation-induced bone loss and parathyroid hormone suppression. J Clin Invest. 2019;129:3058-3071 pubmed publisher
Kamimoto H, Kobayashi Y, Moriyama K. Relaxin 2 carried by magnetically directed liposomes accelerates rat midpalatal suture expansion and subsequent new bone formation. Bone Rep. 2019;10:100202 pubmed publisher
Saito H, Gasser A, Bolamperti S, Maeda M, Matthies L, Jahn K, et al. TG-interacting factor 1 (Tgif1)-deficiency attenuates bone remodeling and blunts the anabolic response to parathyroid hormone. Nat Commun. 2019;10:1354 pubmed publisher
Inoue M, Ono T, Kameo Y, Sasaki F, Ono T, Adachi T, et al. Forceful mastication activates osteocytes and builds a stout jawbone. Sci Rep. 2019;9:4404 pubmed publisher
Wang K, Le L, Chun B, Tiede Lewis L, Shiflett L, Prideaux M, et al. A Novel Osteogenic Cell Line That Differentiates Into GFP-Tagged Osteocytes and Forms Mineral With a Bone-Like Lacunocanalicular Structure. J Bone Miner Res. 2019;34:979-995 pubmed publisher
Supakul S, Yao K, Ochi H, Shimada T, Hashimoto K, Sunamura S, et al. Pericytes as a Source of Osteogenic Cells in Bone Fracture Healing. Int J Mol Sci. 2019;20: pubmed publisher
Kim A, Kim J, Kim A, Sohn Y, Cha J, Bak E, et al. Simvastatin attenuates tibial bone loss in rats with type 1 diabetes and periodontitis. J Transl Med. 2018;16:306 pubmed publisher
Wu Y, Yuan X, Perez K, Hyman S, Wang L, Pellegrini G, et al. Aberrantly elevated Wnt signaling is responsible for cementum overgrowth and dental ankylosis. Bone. 2019;122:176-183 pubmed publisher
Svandová E, Veselá B, Lesot H, Sadoine J, Poliard A, Matalova E. FasL Modulates Expression of Mmp2 in Osteoblasts. Front Physiol. 2018;9:1314 pubmed publisher
Xiao L, Fei Y, Hurley M. FGF2 crosstalk with Wnt signaling in mediating the anabolic action of PTH on bone formation. Bone Rep. 2018;9:136-144 pubmed publisher
Yamaguchi Y, Kumagai K, Imai S, Miyatake K, Saito T. Sclerostin is upregulated in the early stage of chondrogenic differentiation, but not required in endochondral ossification in vitro. PLoS ONE. 2018;13:e0201839 pubmed publisher
Meo Burt P, Xiao L, Hurley M. FGF23 Regulates Wnt/β-Catenin Signaling-Mediated Osteoarthritis in Mice Overexpressing High-Molecular-Weight FGF2. Endocrinology. 2018;159:2386-2396 pubmed publisher
Javaheri B, Carriero A, Wood M, de Souza R, Lee P, Shefelbine S, et al. Transient peak-strain matching partially recovers the age-impaired mechanoadaptive cortical bone response. Sci Rep. 2018;8:6636 pubmed publisher
Narayanan S, Metzger C, Bloomfield S, Zawieja D. Inflammation-induced lymphatic architecture and bone turnover changes are ameliorated by irisin treatment in chronic inflammatory bowel disease. FASEB J. 2018;32:4848-4861 pubmed publisher
Morrell A, Brown G, Robinson S, Sattler R, Baik A, Zhen G, et al. Mechanically induced Ca2+ oscillations in osteocytes release extracellular vesicles and enhance bone formation. Bone Res. 2018;6:6 pubmed publisher
Nakatani T, Chen T, Johnson J, Westendorf J, Partridge N. The Deletion of Hdac4 in Mouse Osteoblasts Influences Both Catabolic and Anabolic Effects in Bone. J Bone Miner Res. 2018;33:1362-1375 pubmed publisher
Gardinier J, Rostami N, Juliano L, Zhang C. Bone adaptation in response to treadmill exercise in young and adult mice. Bone Rep. 2018;8:29-37 pubmed publisher
Sakurai A, Hasegawa T, Kudo A, Shen Z, Nagai T, Abe M, et al. Chronological immunolocalization of sclerostin and FGF23 in the mouse metaphyseal trabecular and cortical bone. Biomed Res. 2017;38:257-267 pubmed publisher
Cui P, Liu H, Sun J, Amizuka N, Sun Q, Li M. Zoledronate promotes bone formation by blocking osteocyte-osteoblast communication during bone defect healing. Histol Histopathol. 2018;33:89-99 pubmed publisher
Ramaswamy G, Kim H, Zhang D, Lounev V, WU J, Choi Y, et al. Gsα Controls Cortical Bone Quality by Regulating Osteoclast Differentiation via cAMP/PKA and β-Catenin Pathways. Sci Rep. 2017;7:45140 pubmed publisher
Ogura K, Iimura T, Makino Y, Sugie Oya A, Takakura A, Takao Kawabata R, et al. Short-term intermittent administration of parathyroid hormone facilitates osteogenesis by different mechanisms in cancellous and cortical bone. Bone Rep. 2016;5:7-14 pubmed publisher
Shu R, Bai D, Sheu T, He Y, Yang X, Xue C, et al. Sclerostin Promotes Bone Remodeling in the Process of Tooth Movement. PLoS ONE. 2017;12:e0167312 pubmed publisher
Metzger C, Narayanan A, Zawieja D, Bloomfield S. Inflammatory Bowel Disease in a Rodent Model Alters Osteocyte Protein Levels Controlling Bone Turnover. J Bone Miner Res. 2017;32:802-813 pubmed publisher
Chandra A, Lin T, Young T, Tong W, Ma X, Tseng W, et al. Suppression of Sclerostin Alleviates Radiation-Induced Bone Loss by Protecting Bone-Forming Cells and Their Progenitors Through Distinct Mechanisms. J Bone Miner Res. 2017;32:360-372 pubmed publisher
Amri N, Djolé S, Petit S, Babajko S, Coudert A, Castaneda B, et al. Distorted Patterns of Dentinogenesis and Eruption in Msx2 Null Mutants: Involvement of Sost/Sclerostin. Am J Pathol. 2016;186:2577-87 pubmed publisher
product information
brand :
R&D Systems
master code :
AF1589
SKU :
AF1589
product name :
Mouse SOST/Sclerostin Antibody
unit size :
100 ug (also 25 ug)
seo description :
The Mouse SOST/Sclerostin Antibody from R&D Systems is a goat polyclonal antibody to SOST/Sclerostin. This antibody reacts with human,mouse,n/a,rat,xenograft. The Mouse SOST/Sclerostin Antibody has been validated for the following applications: Immunohistochemistry,Flow Cytometry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunocytochemistry.
target :
SOST/Sclerostin
category :
Primary Antibodies
buffer :
Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.
clonality :
Polyclonal
concentration :
LYOPH
conjugate :
Unconjugated
dilution :
Western Blot 2 ug/mL, Immunohistochemistry 5-15 ug/mL
host :
Goat
immunogen :
Mouse myeloma cell line NS0-derived recombinant mouse SOST/Sclerostin, Gln24-Tyr211, Accession # NP_077769
isotype :
IgG
purity :
Antigen Affinity-purified
species :
Human,Mouse,N/A,Rat,Xenograft
specificity :
Detects mouse SOST/Sclerostin in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human SOST is observed.
gene symbol :
SOST
top caption :
Detection of Human and Mouse SOST/Sclerostin antibody by Western Blot.
accessionNumbers :
NP_077769
applications :
Flow Cytometry,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunocytochemistry
USD :
499 USD
alt names :
sclerostin, VBCHsclerosteosis
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.
more info or order :
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.