product summary
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company name :
R&D Systems
product type :
antibody
product name :
Human/Mouse/Rat TrkB Antibody
catalog :
AF1494
quantity :
100 ug (also 25 ug)
price :
489 USD
clonality :
polyclonal
host :
domestic goat
conjugate :
nonconjugated
reactivity :
human, mouse, rat, chicken
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, neutralization, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, immunohistochemistry - free floating section
more info or order :
citations: 70
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - frozen section; mouse; 1:100; loading ...; fig 2e
Karpinski B, Maynard T, Bryan C, Yitsege G, Horvath A, Lee N, et al. Selective disruption of trigeminal sensory neurogenesis and differentiation in a mouse model of 22q11.2 deletion syndrome. Dis Model Mech. 2022;15: pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 1a
Annamneedi A, Del Angel M, Gundelfinger E, Stork O, Caliskan G. The Presynaptic Scaffold Protein Bassoon in Forebrain Excitatory Neurons Mediates Hippocampal Circuit Maturation: Potential Involvement of TrkB Signalling. Int J Mol Sci. 2021;22: pubmed publisher
  • immunohistochemistry - free floating section; mouse; 0.5 ug/ml; loading ...; fig 1c
Fiedler D, Sasi M, Blum R, Klinke C, Andreatta M, Pape H, et al. Brain-Derived Neurotrophic Factor/Tropomyosin Receptor Kinase B Signaling Controls Excitability and Long-Term Depression in Oval Nucleus of the BNST. J Neurosci. 2021;41:435-445 pubmed publisher
  • western blot; human; 1:50; loading ...; fig 9g
Fulgenzi G, Hong Z, Tomassoni Ardori F, Barella L, Becker J, Barrick C, et al. Novel metabolic role for BDNF in pancreatic β-cell insulin secretion. Nat Commun. 2020;11:1950 pubmed publisher
  • immunocytochemistry; human; 1:500; fig 2b
Alshawaf A, Viventi S, Qiu W, D Abaco G, Nayagam B, Erlichster M, et al. Phenotypic and Functional Characterization of Peripheral Sensory Neurons derived from Human Embryonic Stem Cells. Sci Rep. 2018;8:603 pubmed publisher
  • immunocytochemistry; mouse
  • immunohistochemistry; mouse; loading ...; fig 7b
Guimarães Camboa N, Cattaneo P, Sun Y, Moore Morris T, Gu Y, Dalton N, et al. Pericytes of Multiple Organs Do Not Behave as Mesenchymal Stem Cells In Vivo. Cell Stem Cell. 2017;20:345-359.e5 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:500; fig 1
Schütze S, Orozco I, Jentsch T. KCNQ Potassium Channels Modulate Sensitivity of Skin Down-hair (D-hair) Mechanoreceptors. J Biol Chem. 2016;291:5566-75 pubmed publisher
Gupta R, Dittmeier M, Wohlleben G, Nickl V, Bischler T, Luzak V, et al. Atypical cellular responses mediated by intracellular constitutive active TrkB (NTRK2) kinase domains and a solely intracellular NTRK2-fusion oncogene. Cancer Gene Ther. 2024;31:1357-1379 pubmed publisher
Kim H, Lee H, Jeon Y, Jang S, Shin Y, Yun J, et al. Targeting SARM1 improves autophagic stress-induced axonal neuropathy. Autophagy. 2024;20:29-44 pubmed publisher
Ayon Olivas M, Wolf D, Andreska T, Granado N, L xfc ningschr xf6 r P, Ip C, et al. Dopaminergic Input Regulates the Sensitivity of Indirect Pathway Striatal Spiny Neurons to Brain-Derived Neurotrophic Factor. Biology (Basel). 2023;12: pubmed publisher
Alitalo O, Gonz xe1 lez Hern xe1 ndez G, Rosenholm M, Kohtala P, Matsui N, M xfc ller H, et al. Linking Hypothermia and Altered Metabolism with TrkB Activation. ACS Chem Neurosci. 2023;14:3212-3225 pubmed publisher
Lopes Rodrigues V, Boxy P, Sim E, Park D, Habeck M, Carbonell J, et al. AraC interacts with p75NTR transmembrane domain to induce cell death of mature neurons. Cell Death Dis. 2023;14:440 pubmed publisher
Moliner R, Girych M, Brunello C, Kovaleva V, Biojone C, Enkavi G, et al. Psychedelics promote plasticity by directly binding to BDNF receptor TrkB. Nat Neurosci. 2023;26:1032-1041 pubmed publisher
Hovhannisyan A, Lindquist K, Belugin S, Mecklenburg J, Ibrahim T, Tram M, et al. Sensory innervation of masseter, temporal and lateral pterygoid muscles in common marmosets. bioRxiv. 2023;: pubmed publisher
Schmidbauer D, Fink S, Rousset F, L xf6 wenheim H, Senn P, Glueckert R. Closing the Gap between the Auditory Nerve and Cochlear Implant Electrodes: Which Neurotrophin Cocktail Performs Best for Axonal Outgrowth and Is Electrical Stimulation Beneficial?. Int J Mol Sci. 2023;24: pubmed publisher
Mohamed O, Abdallah D, Fayez A, Mohamed R, El Abhar H. Morin post-treatment surpassed calpeptin in ameliorating 3-NP-induced cortical neurotoxicity via modulation of glutamate/calpain axis, Kidins220, and BDNF/TrkB/AKT/CREB trajectory. Int Immunopharmacol. 2023;116:109771 pubmed publisher
Biojone C, C Casarotto P, Cannarozzo C, Fred S, Herrera Rodr xed guez R, Lesnikova A, et al. nNOS-induced tyrosine nitration of TRKB impairs BDNF signaling and restrains neuronal plasticity. Prog Neurobiol. 2023;222:102413 pubmed publisher
Hennlein L, Ghanawi H, Gerstner F, Palominos Garc xed a E, Yıldırım E, Saal Bauernschubert L, et al. Plastin 3 rescues cell surface translocation and activation of TrkB in spinal muscular atrophy. J Cell Biol. 2023;222: pubmed publisher
Tosolini A, Sleigh J, Surana S, Rhymes E, Cahalan S, Schiavo G. BDNF-dependent modulation of axonal transport is selectively impaired in ALS. Acta Neuropathol Commun. 2022;10:121 pubmed publisher
Ilchibaeva T, Tsybko A, Zeug A, M xfc ller F, Guseva D, Bischoff S, et al. Serotonin Receptor 5-HT2A Regulates TrkB Receptor Function in Heteroreceptor Complexes. Cells. 2022;11: pubmed publisher
Rodocker H, Bordbar A, Larson M, Biltz R, Wangler L, Fadda P, et al. Breaking Mental Barriers Promotes Recovery After Spinal Cord Injury. Front Mol Neurosci. 2022;15:868563 pubmed publisher
Mitre M, Saadipour K, Williams K, Khatri L, Froemke R, Chao M. Transactivation of TrkB Receptors by Oxytocin and Its G Protein-Coupled Receptor. Front Mol Neurosci. 2022;15:891537 pubmed publisher
Deng C, Reinhard S, Hennlein L, Eilts J, Sachs S, Doose S, et al. Impaired dynamic interaction of axonal endoplasmic reticulum and ribosomes contributes to defective stimulus-response in spinal muscular atrophy. Transl Neurodegener. 2022;11:31 pubmed publisher
Ateaque S, Merkouris S, Wyatt S, Allen N, Xie J, DiStefano P, et al. Selective activation and down-regulation of Trk receptors by neurotrophins in human neurons co-expressing TrkB and TrkC. J Neurochem. 2022;161:463-477 pubmed publisher
Tolman Z, Chaverra M, George L, Lefcort F. Elp1 is required for development of visceral sensory peripheral and central circuitry. Dis Model Mech. 2022;15: pubmed publisher
Chang L, Masada M, Kojima M, Yamamoto N. Involvement of Denervated Midbrain-Derived Factors in the Formation of Ectopic Cortico-Mesencephalic Projection after Hemispherectomy. J Neurosci. 2022;42:749-761 pubmed publisher
Virtuoso A, Tveden Nyborg P, Schou Pedersen A, Lykkesfeldt J, M xfc ller H, Elfving B, et al. A Long-Term Energy-Rich Diet Increases Prefrontal BDNF in Sprague-Dawley Rats. Nutrients. 2021;14: pubmed publisher
Lindquist K, Belugin S, Hovhannisyan A, Corey T, Salmon A, Akopian A. Identification of Trigeminal Sensory Neuronal Types Innervating Masseter Muscle. Eneuro. 2021;8: pubmed publisher
Muzyka V, Badea T. Genetic interplay between transcription factor Pou4f1/Brn3a and neurotrophin receptor Ret in retinal ganglion cell type specification. Neural Dev. 2021;16:5 pubmed publisher
Lesnikova A, Casarotto P, Moliner R, Fred S, Biojone C, Castr xe9 n E. Perineuronal Net Receptor PTPσ Regulates Retention of Memories. Front Synaptic Neurosci. 2021;13:672475 pubmed publisher
De Bacco F, Orzan F, Erriquez J, Casanova E, Barault L, Albano R, et al. ERBB3 overexpression due to miR-205 inactivation confers sensitivity to FGF, metabolic activation, and liability to ERBB3 targeting in glioblastoma. Cell Rep. 2021;36:109455 pubmed publisher
Viventi S, Frausin S, Howden S, Lim S, Finol Urdaneta R, McArthur J, et al. In vivo survival and differentiation of Friedreich ataxia iPSC-derived sensory neurons transplanted in the adult dorsal root ganglia. Stem Cells Transl Med. 2021;10:1157-1169 pubmed publisher
Fleury S, Boukhatem I, Le Blanc J, Welman M, Lordkipanidzé M. Tissue-Specificity of Antibodies Raised Against TrkB and p75NTR Receptors; Implications for Platelets as Models of Neurodegenerative Diseases. Front Immunol. 2021;12:606861 pubmed publisher
Casarotto P, Girych M, Fred S, Kovaleva V, Moliner R, Enkavi G, et al. Antidepressant drugs act by directly binding to TRKB neurotrophin receptors. Cell. 2021;184:1299-1313.e19 pubmed publisher
Sultan N, Amin L, Zaher A, Grawish M, Scheven B. Dental pulp stem cells stimulate neuronal differentiation of PC12 cells. Neural Regen Res. 2021;16:1821-1828 pubmed publisher
Kampanis V, Tolou Dabbaghian B, Zhou L, Roth W, Puttagunta R. Cyclic Stretch of Either PNS or CNS Located Nerves Can Stimulate Neurite Outgrowth. Cells. 2020;10: pubmed publisher
Lesnikova A, Casarotto P, Fred S, Voipio M, Winkel F, Steinzeig A, et al. Chondroitinase and Antidepressants Promote Plasticity by Releasing TRKB from Dephosphorylating Control of PTPσ in Parvalbumin Neurons. J Neurosci. 2021;41:972-980 pubmed publisher
Kartalou G, Salgueiro Pereira A, Endres T, Lesnikova A, Casarotto P, Pousinha P, et al. Anti-Inflammatory Treatment with FTY720 Starting after Onset of Symptoms Reverses Synaptic Deficits in an AD Mouse Model. Int J Mol Sci. 2020;21: pubmed publisher
Sun D, Milibari L, Pan J, Ren X, Yao L, Zhao Y, et al. Critical Roles of Embryonic Born Dorsal Dentate Granule Neurons for Activity-Dependent Increases in BDNF, Adult Hippocampal Neurogenesis, and Antianxiety-like Behaviors. Biol Psychiatry. 2021;89:600-614 pubmed publisher
Faure L, Wang Y, Kastriti M, Fontanet P, Cheung K, Petitpré C, et al. Single cell RNA sequencing identifies early diversity of sensory neurons forming via bi-potential intermediates. Nat Commun. 2020;11:4175 pubmed publisher
Stupack J, Xiong X, Jiang L, Zhang T, Zhou L, Campos A, et al. Soluble SORLA Enhances Neurite Outgrowth and Regeneration through Activation of the EGF Receptor/ERK Signaling Axis. J Neurosci. 2020;40:5908-5921 pubmed publisher
Xiao D, Deng Q, Guo Y, Huang X, Zou M, Zhong J, et al. Generation of self-organized sensory ganglion organoids and retinal ganglion cells from fibroblasts. Sci Adv. 2020;6:eaaz5858 pubmed publisher
Pagella P, Caton J, Meisel C, Mitsiadis T. Ameloblastomas Exhibit Stem Cell Potential, Possess Neurotrophic Properties, and Establish Connections with Trigeminal Neurons. Cells. 2020;9: pubmed publisher
Kim S, Hadley S, Maddison M, Patil M, Cha B, Kollarik M, et al. Mapping of Sensory Nerve Subsets within the Vagal Ganglia and the Brainstem Using Reporter Mice for Pirt, TRPV1, 5-HT3, and Tac1 Expression. Eneuro. 2020;7: pubmed publisher
Gómez Fernández P, Lopez de Lapuente Portilla A, Astobiza I, Mena J, Urtasun A, Altmann V, et al. The Rare IL22RA2 Signal Peptide Coding Variant rs28385692 Decreases Secretion of IL-22BP Isoform-1, -2 and -3 and Is Associated with Risk for Multiple Sclerosis. Cells. 2020;9: pubmed publisher
Moon G, Kim S, Jeon M, Lee K, Jang I, Nakamura M, et al. Therapeutic Potential of AAV1-Rheb(S16H) Transduction Against Alzheimer's Disease. J Clin Med. 2019;8: pubmed publisher
Jeon M, Moon G, Kim S, Choi M, Oh Y, Kim D, et al. Neurotrophic interactions between neurons and astrocytes following AAV1-Rheb(S16H) transduction in the hippocampus in vivo. Br J Pharmacol. 2020;177:668-686 pubmed publisher
Fred S, Laukkanen L, Brunello C, Vesa L, Göös H, Cardon I, et al. Pharmacologically diverse antidepressants facilitate TRKB receptor activation by disrupting its interaction with the endocytic adaptor complex AP-2. J Biol Chem. 2019;294:18150-18161 pubmed publisher
Paixão S, Loschek L, Gaitanos L, Alcalà Morales P, Goulding M, Klein R. Identification of Spinal Neurons Contributing to the Dorsal Column Projection Mediating Fine Touch and Corrective Motor Movements. Neuron. 2019;104:749-764.e6 pubmed publisher
Wang Y, Wu H, Zelenin P, Fontanet P, Wanderoy S, Petitpré C, et al. Muscle-selective RUNX3 dependence of sensorimotor circuit development. Development. 2019;146: pubmed publisher
Wang Y, Wu H, Fontanet P, Codeluppi S, Akkuratova N, Petitpré C, et al. A cell fitness selection model for neuronal survival during development. Nat Commun. 2019;10:4137 pubmed publisher
Acton D, Ren X, Di Costanzo S, Dalet A, Bourane S, Bertocchi I, et al. Spinal Neuropeptide Y1 Receptor-Expressing Neurons Form an Essential Excitatory Pathway for Mechanical Itch. Cell Rep. 2019;28:625-639.e6 pubmed publisher
Patil M, Hovhannisyan A, Wangzhou A, Mecklenburg J, Koek W, Goffin V, et al. Prolactin receptor expression in mouse dorsal root ganglia neuronal subtypes is sex-dependent. J Neuroendocrinol. 2019;31:e12759 pubmed publisher
Richner M, Pallesen L, Ulrichsen M, Poulsen E, Holm T, Login H, et al. Sortilin gates neurotensin and BDNF signaling to control peripheral neuropathic pain. Sci Adv. 2019;5:eaav9946 pubmed publisher
Wiszniak S, Schwarz Q. Notch signalling defines dorsal root ganglia neuroglial fate choice during early neural crest cell migration. BMC Neurosci. 2019;20:21 pubmed publisher
Takeoka A, Arber S. Functional Local Proprioceptive Feedback Circuits Initiate and Maintain Locomotor Recovery after Spinal Cord Injury. Cell Rep. 2019;27:71-85.e3 pubmed publisher
Desiderio S, Vermeiren S, Van Campenhout C, Kricha S, Malki E, Richts S, et al. Prdm12 Directs Nociceptive Sensory Neuron Development by Regulating the Expression of the NGF Receptor TrkA. Cell Rep. 2019;26:3522-3536.e5 pubmed publisher
Bartesaghi L, Wang Y, Fontanet P, Wanderoy S, Berger F, Wu H, et al. PRDM12 Is Required for Initiation of the Nociceptive Neuron Lineage during Neurogenesis. Cell Rep. 2019;26:3484-3492.e4 pubmed publisher
Diniz C, Biojone C, Joca S, Rantamäki T, Castren E, Guimarães F, et al. Dual mechanism of TRKB activation by anandamide through CB1 and TRPV1 receptors. Peerj. 2019;7:e6493 pubmed publisher
Zhang M, Wang Y, Geng J, Zhou S, Xiao B. Mechanically Activated Piezo Channels Mediate Touch and Suppress Acute Mechanical Pain Response in Mice. Cell Rep. 2019;26:1419-1431.e4 pubmed publisher
Sar Shalom H, Goldner R, Golan Vaishenker Y, Yaron A. Balance between BDNF and Semaphorins gates the innervation of the mammary gland. elife. 2019;8: pubmed publisher
Patil M, Hovhannisyan A, Akopian A. Characteristics of sensory neuronal groups in CGRP-cre-ER reporter mice: Comparison to Nav1.8-cre, TRPV1-cre and TRPV1-GFP mouse lines. PLoS ONE. 2018;13:e0198601 pubmed publisher
Pérez Rando M, Castillo Gomez E, Bueno Fernandez C, Nacher J. The TrkB agonist 7,8-dihydroxyflavone changes the structural dynamics of neocortical pyramidal neurons and improves object recognition in mice. Brain Struct Funct. 2018;223:2393-2408 pubmed publisher
Kim S, Moon G, Oh Y, Park J, Shin W, Jeong J, et al. Protection of nigral dopaminergic neurons by AAV1 transduction with Rheb(S16H) against neurotoxic inflammation in vivo. Exp Mol Med. 2018;50:e440 pubmed publisher
Bernal Sierra Y, Haseleu J, Kozlenkov A, Begay V, Lewin G. Genetic Tracing of Cav3.2 T-Type Calcium Channel Expression in the Peripheral Nervous System. Front Mol Neurosci. 2017;10:70 pubmed publisher
Chen K, Zhang L, Tan M, Lai C, Li A, Ren C, et al. Treadmill exercise suppressed stress-induced dendritic spine elimination in mouse barrel cortex and improved working memory via BDNF/TrkB pathway. Transl Psychiatry. 2017;7:e1069 pubmed publisher
Price E, Krasowska Zoladek A, Nanda K, Stachel S, Henze D. Development of a pharmacodynamic biomarker to measure target engagement from inhibition of the NGF-TrkA pathway. J Neurosci Methods. 2017;282:34-42 pubmed publisher
Lao Peregrín C, Ballesteros J, Fernández M, Zamora Moratalla A, Saavedra A, Gomez Lazaro M, et al. Caffeine-mediated BDNF release regulates long-term synaptic plasticity through activation of IRS2 signaling. Addict Biol. 2017;22:1706-1718 pubmed publisher
Reed Geaghan E, Wright M, See L, Adelman P, Lee K, Koerber H, et al. Merkel Cell-Driven BDNF Signaling Specifies SAI Neuron Molecular and Electrophysiological Phenotypes. J Neurosci. 2016;36:4362-76 pubmed publisher
Usoskin D, Furlan A, Islam S, Abdo H, Lönnerberg P, Lou D, et al. Unbiased classification of sensory neuron types by large-scale single-cell RNA sequencing. Nat Neurosci. 2015;18:145-53 pubmed publisher
product information
master code :
AF1494
SKU :
AF1494
product name :
Human/Mouse/Rat TrkB Antibody
unit size :
100 ug (also 25 ug)
description :
The Human/Mouse/Rat TrkB Antibody from R&D Systems is a goat polyclonal antibody to TrkB. This antibody reacts with avian - chicken,human,mouse,primate,primate - callithrix jacchus (common marmoset),rat,transgenic mouse. The Human/Mouse/Rat TrkB Antibody has been validated for the following applications: Immunohistochemistry,Western Blot,IHC fixed frozen,Bioassay,ELISA Development (Capture),IHC-F,Flow Cytometry,Immunoprecipitation,Neutralization,ELISA Capture,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunocytochemistry,Simple Western,Blockade of Receptor-ligand Interaction.
target :
TrkB
category :
Primary Antibodies
buffer :
Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.
clonality :
Polyclonal
concentration :
LYOPH
conjugate :
Unconjugated
host :
Goat
immunogen :
Mouse myeloma cell line NS0-derived recombinant mouse TrkB, Cys32-His429, Accession # P15209
isotype :
IgG
purity :
Antigen Affinity-purified
species :
Avian - Chicken,Human,Mouse,Primate,Primate - Callithrix jacchus (Common Marmoset),Rat,Transgenic Mouse
specificity :
Detects mouse TrkB in direct ELISAs. Detects human, mouse, and rat TrkB in Western blots.
gene symbol :
NTRK2
endotoxin note :
<0.10 EU per 1 ╡g of the antibody by the LAL method.
top caption :
Detection of Human, Mouse, and Rat TrkB antibody by Western Blot.
accessionNumbers :
P15209
applications :
Western Blot,IHC fixed frozen,Immunohistochemistry,Bioassay,ELISA Development (Capture),IHC-F,Flow Cytometry,Immunoprecipitation,Neutralization,ELISA Capture,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunocytochemistry,Simple Western,Blockade of Receptor-ligand Interaction
USD :
489 USD
alt names :
BDNF/NT-3 growth factors receptor, EC 2.7.10, EC 2.7.10.1, GP145-TrkB, Neurotrophic tyrosine kinase receptor type 2, neurotrophic tyrosine kinase, receptor, type 2, NTRK2, trk-B, TrkB tyrosine kinase, TRKBBDNF-tropomyosine receptor kinase B, tyrosine kinase receptor B
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.
more info or order :
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.