product summary
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company name :
R&D Systems
product type :
antibody
product name :
Human Osteopontin/OPN Antibody
catalog :
AF1433
quantity :
100 ug (also 25 ug)
price :
489 USD
clonality :
polyclonal
host :
domestic goat
conjugate :
nonconjugated
reactivity :
human, mouse, rat
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, neutralization, flow cytometry, immunohistochemistry - paraffin section
more info or order :
R&D Systems product webpage
citations: 32
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; human; loading ...; fig 3a
Lu C, Liu Z, Klement J, Yang D, Merting A, Poschel D, et al. WDR5-H3K4me3 epigenetic axis regulates OPN expression to compensate PD-L1 function to promote pancreatic cancer immune escape. J Immunother Cancer. 2021;9: pubmed publisher
Wang X, Ramos R, Phan A, Yamaga K, Flesher J, Jiang S, et al. Signalling by senescent melanocytes hyperactivates hair growth. Nature. 2023;618:808-817 pubmed publisher
Lad B, Beniwal A, Jain S, Shukla P, Jung J, Shah S, et al. Glioblastoma induces the recruitment and differentiation of hybrid neutrophils from skull bone marrow. bioRxiv. 2023;: pubmed publisher
Eun J, Yoon J, Ahn H, Kim S, Kim Y, Lim S, et al. Cancer-associated fibroblast-derived secreted phosphoprotein 1 contributes to resistance of hepatocellular carcinoma to sorafenib and lenvatinib. Cancer Commun (Lond). 2023;43:455-479 pubmed publisher
Kazakova E, Rakina M, Sudarskikh T, Iamshchikov P, Tarasova A, Tashireva L, et al. Angiogenesis regulators S100A4, SPARC and SPP1 correlate with macrophage infiltration and are prognostic biomarkers in colon and rectal cancers. Front Oncol. 2023;13:1058337 pubmed publisher
Li H, Br xe4 unig S, Dhapolar P, Karlsson G, Lang S, Scheding S. Identification of phenotypically, functionally, and anatomically distinct stromal niche populations in human bone marrow based on single-cell RNA sequencing. elife. 2023;12: pubmed publisher
Zhou Y, Tada M, Cai Z, Andhey P, Swain A, Miller K, et al. Human early-onset dementia caused by DAP12 deficiency reveals a unique signature of dysregulated microglia. Nat Immunol. 2023;24:545-557 pubmed publisher
Matsubara E, Komohara Y, Esumi S, Shinchi Y, Ishizuka S, Mito R, et al. SPP1 Derived from Macrophages Is Associated with a Worse Clinical Course and Chemo-Resistance in Lung Adenocarcinoma. Cancers (Basel). 2022;14: pubmed publisher
Sui Y, Hu W, Zhang W, Li D, Zhu H, You Q, et al. Insights into homeobox B9: a propeller for metastasis in dormant prostate cancer progenitor cells. Br J Cancer. 2021;125:1003-1015 pubmed publisher
Hajal C, Shin Y, Li L, Serrano J, Jacks T, Kamm R. The CCL2-CCR2 astrocyte-cancer cell axis in tumor extravasation at the brain. Sci Adv. 2021;7: pubmed publisher
Park J, Park S, Kim J, Jang K, Seonwoo H, Chung J. Enhanced Osteogenic Differentiation of Periodontal Ligament Stem Cells Using a Graphene Oxide-Coated Poly(ε-caprolactone) Scaffold. Polymers (Basel). 2021;13: pubmed publisher
Viventi S, Frausin S, Howden S, Lim S, Finol Urdaneta R, McArthur J, et al. In vivo survival and differentiation of Friedreich ataxia iPSC-derived sensory neurons transplanted in the adult dorsal root ganglia. Stem Cells Transl Med. 2021;10:1157-1169 pubmed publisher
Tokuda K, Morine Y, Miyazaki K, Yamada S, Saito Y, Nishi M, et al. The interaction between cancer associated fibroblasts and tumor associated macrophages via the osteopontin pathway in the tumor microenvironment of hepatocellular carcinoma. Oncotarget. 2021;12:333-343 pubmed publisher
Lim J, Jang K, Son H, Park S, Kim J, Kim H, et al. Aligned Nanofiber-Guided Bone Regeneration Barrier Incorporated with Equine Bone-Derived Hydroxyapatite for Alveolar Bone Regeneration. Polymers (Basel). 2020;13: pubmed publisher
Gao X, Jia G, Guttman A, DePianto D, Morshead K, Sun K, et al. Osteopontin Links Myeloid Activation and Disease Progression in Systemic Sclerosis. Cell Rep Med. 2020;1:100140 pubmed publisher
Bruijn L, van den Akker B, van Rhijn C, Hamming J, Lindeman J. Extreme Diversity of the Human Vascular Mesenchymal Cell Landscape. J Am Heart Assoc. 2020;9:e017094 pubmed publisher
Tan X, Shi L, Banerjee P, Liu X, Guo H, Yu J, et al. A protumorigenic secretory pathway activated by p53 deficiency in lung adenocarcinoma. J Clin Invest. 2021;131: pubmed publisher
Haraguchi T, Ayukawa Y, Shibata Y, Takeshita T, Atsuta I, Ogino Y, et al. Effect of Calcium Chloride Hydrothermal Treatment of Titanium on Protein, Cellular, and Bacterial Adhesion Properties. J Clin Med. 2020;9: pubmed publisher
Beguier F, Housset M, Roubeix C, Augustin S, Zagar Y, Nous C, et al. The 10q26 Risk Haplotype of Age-Related Macular Degeneration Aggravates Subretinal Inflammation by Impairing Monocyte Elimination. Immunity. 2020;53:429-441.e8 pubmed publisher
Dion xed sio de Sousa I, Marques D, Pr xed ncipe C, Portugal R, Canberk S, Prazeres H, et al. Predictive Biomarkers and Patient Outcome in Platinum-Resistant (PLD-Treated) Ovarian Cancer. Diagnostics (Basel). 2020;10: pubmed publisher
Sadaghianloo N, Contenti J, Dufies M, Parola J, Rouleau M, Lee S, et al. Co-culture of human fibroblasts, smooth muscle and endothelial cells promotes osteopontin induction in hypoxia. J Cell Mol Med. 2020;24:2931-2941 pubmed publisher
Weiss Bilka H, Meagher M, Gargac J, Niebur G, Roeder R, Wagner D. Mineral deposition and vascular invasion of hydroxyapatite reinforced collagen scaffolds seeded with human adipose-derived stem cells. Biomater Res. 2019;23:15 pubmed publisher
Zhang Y, Williams P, Jacobi A, Wang C, Goel A, Hirano A, et al. Elevating Growth Factor Responsiveness and Axon Regeneration by Modulating Presynaptic Inputs. Neuron. 2019;: pubmed publisher
Kapelouzou A, Kontogiannis C, Tsilimigras D, Georgiopoulos G, Kaklamanis L, Tsourelis L, et al. Differential expression patterns of Toll Like Receptors and Interleukin-37 between calcific aortic and mitral valve cusps in humans. Cytokine. 2019;116:150-160 pubmed publisher
Wei J, Marisetty A, Schrand B, Gabrusiewicz K, Hashimoto Y, Ott M, et al. Osteopontin mediates glioblastoma-associated macrophage infiltration and is a potential therapeutic target. J Clin Invest. 2019;129:137-149 pubmed publisher
Cochain C, Vafadarnejad E, Arampatzi P, Pelisek J, Winkels H, Ley K, et al. Single-Cell RNA-Seq Reveals the Transcriptional Landscape and Heterogeneity of Aortic Macrophages in Murine Atherosclerosis. Circ Res. 2018;122:1661-1674 pubmed publisher
Zhao G, Hu M, Li C, Lee J, Yuan K, Zhu G, et al. Osteopontin contributes to effective neutrophil recruitment, IL-1β production and apoptosis in Aspergillus fumigatus keratitis. Immunol Cell Biol. 2018;96:401-412 pubmed publisher
Alshawaf A, Viventi S, Qiu W, D Abaco G, Nayagam B, Erlichster M, et al. Phenotypic and Functional Characterization of Peripheral Sensory Neurons derived from Human Embryonic Stem Cells. Sci Rep. 2018;8:603 pubmed publisher
Chen Y, Xu H, Lin G. Generation of iPSC-derived limb progenitor-like cells for stimulating phalange regeneration in the adult mouse. Cell Discov. 2017;3:17046 pubmed publisher
Coyac B, Hoac B, Chafey P, Falgayrac G, Slimani L, Rowe P, et al. Defective Mineralization in X-Linked Hypophosphatemia Dental Pulp Cell Cultures. J Dent Res. 2018;97:184-191 pubmed publisher
Jakovac H, Grubić Kezele T, Sućurović S, Mulac Jericevic B, Radosević Stasić B. Osteopontin-metallothionein I/II interactions in experimental autoimmune encephalomyelitis. Neuroscience. 2017;350:133-145 pubmed publisher
Jürets A, Le Bras M, Staffler G, Stein G, Leitner L, Neuhofer A, et al. Inhibition of Cellular Adhesion by Immunological Targeting of Osteopontin Neoepitopes Generated through Matrix Metalloproteinase and Thrombin Cleavage. PLoS ONE. 2016;11:e0148333 pubmed publisher
product information
brand :
R&D Systems
master code :
AF1433
SKU :
AF1433
product name :
Human Osteopontin/OPN Antibody
unit size :
100 ug (also 25 ug)
seo description :
The Human Osteopontin/OPN Antibody from R&D Systems is a goat polyclonal antibody to Osteopontin/OPN. This antibody reacts with human,mouse,n/a,rat,xenograft. The Human Osteopontin/OPN Antibody has been validated for the following applications: Immunohistochemistry,Flow Cytometry,Flourescense Microscopy,Protein Adsorption,Western Blot,Neutralization,Functional Assay,ELISA Capture,Immunohistochemistry-Paraffin,Immunocytochemistry,ELISA,Dual RNAscope ISH-IHC Compatible.
target :
Osteopontin/OPN
category :
Primary Antibodies
buffer :
Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.
clonality :
Polyclonal
concentration :
LYOPH
conjugate :
Unconjugated
dilution :
ELISA, Immunohistochemistry 5-15 ug/mL, Neutralization 0.300 - 3.60 ug/mL, Immunocytochemistry 1-15 ug/mL, Dual RNAscope ISH-IHC 5-15 ug/mL
host :
Goat
immunogen :
Human milk-derived Osteopontin/OPN
isotype :
IgG
purity :
Antigen Affinity-purified
species :
Human,Mouse,N/A,Rat,Xenograft
specificity :
Detects human Osteopontin/OPN in direct ELISAs and Western blots.
gene symbol :
SPP1
endotoxin note :
<0.10 EU per 1 ╡g of the antibody by the LAL method.
top caption :
Osteopontin/OPN antibody in HepG2 Human Cell Line by Immunocytochemistry (ICC).
applications :
ELISA,Dual RNAscope ISH-IHC Compatible,Flourescense Microscopy,Protein Adsorption,Flow Cytometry,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Neutralization,Immunocytochemistry,Functional Assay,ELISA Capture
USD :
489 USD
alt names :
BNSP, Bone sialoprotein 1, Eta-1, MGC110940, Nephropontin, OPN, osteopontin, secreted phosphoprotein 1bone sialoprotein I, early T-lymphocyteactivation 1), secreted phosphoprotein-1 (osteopontin, bone sialoprotein), Spp1, SPP-1, SPP1/CALPHA1 fusion, Urinary stone protein, uropontin
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.
more info or order :
R&D Systems product webpage
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.