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company name :
R&D Systems
product type :
antibody
product name :
Human/Mouse CD117/c-kit Antibody
catalog :
AF1356
quantity :
100 ug (also 25 ug)
price :
499 USD
clonality :
polyclonal
host :
domestic goat
conjugate :
nonconjugated
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry, immunocytochemistry, neutralization, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
citations: 78
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; fig s3
Chen Y, Lu C, Cheng W, Kuo K, Yu C, Ho H, et al. An experimental model for ovarian cancer: propagation of ovarian cancer initiating cells and generation of ovarian cancer organoids. BMC Cancer. 2022;22:967 pubmed publisher
  • western blot; mouse; 1:500; loading ...; fig 5b
Dong F, Chen M, Jiang L, Shen Z, Ma L, Han C, et al. PRMT5 Is Involved in Spermatogonial Stem Cells Maintenance by Regulating Plzf Expression via Modulation of Lysine Histone Modifications. Front Cell Dev Biol. 2021;9:673258 pubmed publisher
Iga T, Kobayashi H, Kusumoto D, Sanosaka T, Fujita N, Tai Nagara I, et al. Spatial heterogeneity of bone marrow endothelial cells unveils a distinct subtype in the epiphysis. Nat Cell Biol. 2023;25:1415-1425 pubmed publisher
Lei W, Li Y, Du Z, Su R, Meng T, Ning Y, et al. SRSF1-mediated alternative splicing is required for spermatogenesis. Int J Biol Sci. 2023;19:4883-4897 pubmed publisher
Li X, Ji S, Cipriani G, Hillestad M, Eisenman S, Barry M, et al. Adeno-associated virus-9 reverses delayed gastric emptying of solids in diabetic mice. Neurogastroenterol Motil. 2023;35:e14669 pubmed publisher
Chi R, Xu X, Li J, Xu X, Hu G, Brown P, et al. WNK1 is required during male pachynema to sustain fertility. iScience. 2023;26:107616 pubmed publisher
Grockowiak E, Korn C, Rak J, Lysenko V, Hallou A, Panvini F, et al. Different niches for stem cells carrying the same oncogenic driver affect pathogenesis and therapy response in myeloproliferative neoplasms. Nat Cancer. 2023;4:1193-1209 pubmed publisher
Hansen S, Larsen H, Pikkupeura L, Maciag G, Guiu J, M xfc ller I, et al. An organoid-based CRISPR-Cas9 screen for regulators of intestinal epithelial maturation and cell fate. Sci Adv. 2023;9:eadg4055 pubmed publisher
Zhang X, Liu Y, Sosa F, Gunewardena S, Crawford P, Zielen A, et al. Transcriptional metabolic reprogramming implements meiotic fate decision in mouse testicular germ cells. Cell Rep. 2023;42:112749 pubmed publisher
Liu C, Wang L, Li Y, Guo M, Hu J, Wang T, et al. RNase H1 facilitates recombinase recruitment by degrading DNA-RNA hybrids during meiosis. Nucleic Acids Res. 2023;51:7357-7375 pubmed publisher
Truong Thuy Nguyen V, Taheri N, Choi E, Kellogg T, Linden D, Hayashi Y. Insulin-Like Growth Factor1 Preserves Gastric Pacemaker Cells and Motor Function in Aging via ERK1/2 Activation. Cell Mol Gastroenterol Hepatol. 2023;16:369-383 pubmed publisher
Nakajima Takagi Y, Oshima M, Takano J, Koide S, Itokawa N, Uemura S, et al. Polycomb repressive complex 1.1 coordinates homeostatic and emergency myelopoiesis. elife. 2023;12: pubmed publisher
Hao X, Shen Y, Chen N, Zhang W, Valverde E, Wu L, et al. Osteoprogenitor-GMP crosstalk underpins solid tumor-induced systemic immunosuppression and persists after tumor removal. Cell Stem Cell. 2023;30:648-664.e8 pubmed publisher
Peng Y, Tang X, Shu H, Dong W, Shao H, Zhou B. Sertoli cells are the source of stem cell factor for spermatogenesis. Development. 2023;150: pubmed publisher
Uchida A, Imaimatsu K, Suzuki H, Han X, Ushioda H, Uemura M, et al. SOX17-positive rete testis epithelium is required for Sertoli valve formation and normal spermiogenesis in the male mouse. Nat Commun. 2022;13:7860 pubmed publisher
Klein Rodewald T, Micklich K, Sanz Moreno A, Tost M, Calzada Wack J, Adler T, et al. New C3H KitN824K/WT cancer mouse model develops late-onset malignant mammary tumors with high penetrance. Sci Rep. 2022;12:19793 pubmed publisher
Zheng Z, He H, Tang X, Zhang H, Gou F, Yang H, et al. Uncovering the emergence of HSCs in the human fetal bone marrow by single-cell RNA-seq analysis. Cell Stem Cell. 2022;29:1562-1579.e7 pubmed publisher
Kirsanov O, Johnson T, Malachowski T, Niedenberger B, Gilbert E, Bhowmick D, et al. Modeling mammalian spermatogonial differentiation and meiotic initiation in vitro. Development. 2022;149: pubmed publisher
Peart N, Johnson T, Lee S, Sears M, Yang F, Quesnel Valli xe8 res M, et al. The germ cell-specific RNA binding protein RBM46 is essential for spermatogonial differentiation in mice. PLoS Genet. 2022;18:e1010416 pubmed publisher
Mallick R, Gurzeler E, Toivanen P, Nieminen T, Yl xe4 Herttuala S. Novel Designed Proteolytically Resistant VEGF-B186R127S Promotes Angiogenesis in Mouse Heart by Recruiting Endothelial Progenitor Cells. Front Bioeng Biotechnol. 2022;10:907538 pubmed publisher
Wang L, Yuan P, Challis C, Ravindra Kumar S, Tach xe9 Y. Transduction of Systemically Administered Adeno-Associated Virus in the Colonic Enteric Nervous System and c-Kit Cells of Adult Mice. Front Neuroanat. 2022;16:884280 pubmed publisher
Moody S, Whiley P, Western P, Loveland K. The Impact of Activin A on Fetal Gonocytes: Chronic Versus Acute Exposure Outcomes. Front Endocrinol (Lausanne). 2022;13:896747 pubmed publisher
Hirano K, Nonami Y, Nakamura Y, Sato T, Sato T, Ishiguro K, et al. Temperature sensitivity of DNA double-strand break repair underpins heat-induced meiotic failure in mouse spermatogenesis. Commun Biol. 2022;5:504 pubmed publisher
Chen X, Li S, Liu X, Zhao J, Wu L, You R, et al. Stimulation of C-Kit+ Retinal Progenitor Cells by Stem Cell Factor Confers Protection Against Retinal Degeneration. Front Pharmacol. 2022;13:796380 pubmed publisher
Klingler S, Hsu K, Hua G, Martin M, Adileh M, Baslan T, et al. Disruption of the crypt niche promotes outgrowth of mutated colorectal tumor stem cells. JCI Insight. 2022;7: pubmed publisher
Heil J, Olsavszky V, Busch K, Klapproth K, de la Torre C, Sticht C, et al. Bone marrow sinusoidal endothelium controls terminal erythroid differentiation and reticulocyte maturation. Nat Commun. 2021;12:6963 pubmed publisher
Young I, Wu B, Andricovich J, Chuang S, Li R, Tzatsos A, et al. Differentiation of fetal hematopoietic stem cells requires ARID4B to restrict autocrine KITLG/KIT-Src signaling. Cell Rep. 2021;37:110036 pubmed publisher
Heinrich A, Bhandary B, Potter S, Ratner N, DeFalco T. Cdc42 activity in Sertoli cells is essential for maintenance of spermatogenesis. Cell Rep. 2021;37:109885 pubmed publisher
Progatzky F, Shapiro M, Chng S, García Cassani B, Classon C, Sevgi S, et al. Regulation of intestinal immunity and tissue repair by enteric glia. Nature. 2021;599:125-130 pubmed publisher
Kimura K, Breitbach M, Schildberg F, Hesse M, Fleischmann B. Bone marrow CD73+ mesenchymal stem cells display increased stemness in vitro and promote fracture healing in vivo. Bone Rep. 2021;15:101133 pubmed publisher
Hwang S, Wang J, Lee J, Lee H, Choi T, Choi S, et al. Yeokwisan, a Standardized Herbal Formula, Enhances Gastric Emptying via Modulation of the Ghrelin Pathway in a Loperamide-induced Functional Dyspepsia Mouse Model. Front Pharmacol. 2021;12:753153 pubmed publisher
Aschacher T, Schmidt K, Aschacher O, Eichmair E, Baranyi U, Winkler B, et al. Telocytes in the human ascending aorta: Characterization and exosome-related KLF-4/VEGF-A expression. J Cell Mol Med. 2021;25:9697-9709 pubmed publisher
Codino A, Turowski T, van de Lagemaat L, Ivanova I, Tavosanis A, Much C, et al. NANOS2 is a sequence-specific mRNA-binding protein that promotes transcript degradation in spermatogonial stem cells. iScience. 2021;24:102762 pubmed publisher
Rivero Hinojosa S, Pugacheva E, Kang S, Mendez Catala C, Kovalchuk A, Strunnikov A, et al. The combined action of CTCF and its testis-specific paralog BORIS is essential for spermatogenesis. Nat Commun. 2021;12:3846 pubmed publisher
Jelínková S, Sleiman Y, Fojtik P, Aimond F, Finan A, Hugon G, et al. Dystrophin Deficiency Causes Progressive Depletion of Cardiovascular Progenitor Cells in the Heart. Int J Mol Sci. 2021;22: pubmed publisher
Matsumoto K, Suenaga M, Mizutani Y, Matsui K, Yoshida A, Nakamoto T, et al. Role of transient receptor potential vanilloid subtype 2 in lower oesophageal sphincter in rat acid reflux oesophagitis. J Pharmacol Sci. 2021;146:125-135 pubmed publisher
Lee S, Kim J, Cong X, Yu G, Ryu J, Park K. Efficient Surface Immobilization of Chemically Modified Hyaluronans for Enhanced Bioactivity and Survival of In Vitro-Cultured Embryonic Salivary Gland Mesenchymal Cells. Polymers (Basel). 2021;13: pubmed publisher
Habara O, Logan C, Kanai Azuma M, Nusse R, Takase H. WNT signaling in pre-granulosa cells is required for ovarian folliculogenesis and female fertility. Development. 2021;148: pubmed publisher
Jia W, Kong L, Kidoya H, Naito H, Muramatsu F, Hayashi Y, et al. Indispensable role of Galectin-3 in promoting quiescence of hematopoietic stem cells. Nat Commun. 2021;12:2118 pubmed publisher
Suzuki S, McCarrey J, Hermann B. Differential RA responsiveness among subsets of mouse late progenitor spermatogonia. Reproduction. 2021;161:645-655 pubmed publisher
Suzuki S, McCarrey J, Hermann B. An mTORC1-dependent switch orchestrates the transition between mouse spermatogonial stem cells and clones of progenitor spermatogonia. Cell Rep. 2021;34:108752 pubmed publisher
Guimbal S, Cornuault L, Rouault P, Hollier P, Chapouly C, Bats M, et al. Mast Cells Are the Trigger of Small Vessel Disease and Diastolic Dysfunction in Diabetic Obese Mice. Arterioscler Thromb Vasc Biol. 2021;41:e193-e207 pubmed publisher
Imura Kishi K, Uchida A, Tsunekawa N, Suzuki H, Takase H, Hirate Y, et al. Low retinoic acid levels mediate regionalization of the Sertoli valve in the terminal segment of mouse seminiferous tubules. Sci Rep. 2021;11:1110 pubmed publisher
Baker S, Leigh W, Del Valle G, De Yturriaga I, Ward S, Cobine C, et al. Ca2+ signaling driving pacemaker activity in submucosal interstitial cells of Cajal in the murine colon. elife. 2021;10: pubmed publisher
Hou L, Voit R, Sankaran V, Springer T, Yuki K. CD11c regulates hematopoietic stem and progenitor cells under stress. Blood Adv. 2020;4:6086-6097 pubmed publisher
Su T, Yang Y, Lai S, Jeong J, Jung Y, McConnell M, et al. Single-Cell Transcriptomics Reveals Zone-Specific Alterations of Liver Sinusoidal Endothelial Cells in Cirrhosis. Cell Mol Gastroenterol Hepatol. 2021;11:1139-1161 pubmed publisher
Wanigasuriya I, Gouil Q, Kinkel S, Tapia Del Fierro A, Beck T, Roper E, et al. Smchd1 is a maternal effect gene required for genomic imprinting. elife. 2020;9: pubmed publisher
Apóstolo N, Smukowski S, Vanderlinden J, Condomitti G, Rybakin V, Ten Bos J, et al. Synapse type-specific proteomic dissection identifies IgSF8 as a hippocampal CA3 microcircuit organizer. Nat Commun. 2020;11:5171 pubmed publisher
Crosse E, Gordon Keylock S, Rybtsov S, Binagui Casas A, Felchle H, Nnadi N, et al. Multi-layered Spatial Transcriptomics Identify Secretory Factors Promoting Human Hematopoietic Stem Cell Development. Cell Stem Cell. 2020;27:822-839.e8 pubmed publisher
Sogawa Fujiwara C, Hanagata A, Fujiwara Y, Ishida Y, Tomiyasu H, Kunieda T, et al. Defective development and microcirculation of intestine in Npr2 mutant mice. Sci Rep. 2020;10:14761 pubmed publisher
Ebeid D, Firouzi F, Esquer C, Navarrete J, Wang B, Gude N, et al. PIM1 Promotes Survival of Cardiomyocytes by Upregulating c-Kit Protein Expression. Cells. 2020;9: pubmed publisher
Hayashi Y, Asuzu D, Bardsley M, Gajdos G, Kvasha S, Linden D, et al. Wnt-induced, TRP53-mediated Cell Cycle Arrest of Precursors Underlies Interstitial Cell of Cajal Depletion During Aging. Cell Mol Gastroenterol Hepatol. 2021;11:117-145 pubmed publisher
Mikedis M, Fan Y, Nicholls P, Endo T, Jackson E, Cobb S, et al. DAZL mediates a broad translational program regulating expansion and differentiation of spermatogonial progenitors. elife. 2020;9: pubmed publisher
Stringer J, Winship A, Zerafa N, Wakefield M, Hutt K. Oocytes can efficiently repair DNA double-strand breaks to restore genetic integrity and protect offspring health. Proc Natl Acad Sci U S A. 2020;117:11513-11522 pubmed publisher
Heinrich A, Potter S, Guo L, Ratner N, DeFalco T. Distinct Roles for Rac1 in Sertoli Cell Function during Testicular Development and Spermatogenesis. Cell Rep. 2020;31:107513 pubmed publisher
Liu C, Ma Z, Cai Z, Zhang F, Liu C, Chen T, et al. Identification of primordial germ cell-like cells as liver metastasis initiating cells in mouse tumour models. Cell Discov. 2020;6:15 pubmed publisher
CHALAZONITIS A, Li Z, Pham T, Chen J, Rao M, Lindholm P, et al. Cerebral dopamine neurotrophic factor is essential for enteric neuronal development, maintenance, and regulation of gastrointestinal transit. J Comp Neurol. 2020;528:2420-2444 pubmed publisher
Hou M, Han J, Li G, Kwon M, Jiang J, Emani S, et al. Multipotency of mouse trophoblast stem cells. Stem Cell Res Ther. 2020;11:55 pubmed publisher
Tillman H, Janke L, Funk A, Vogel P, Rehg J. Morphologic and Immunohistochemical Characterization of Spontaneous Lymphoma/Leukemia in NSG Mice. Vet Pathol. 2020;57:160-171 pubmed publisher
So E, Sun C, Wu K, Driesman A, Leggett S, Isaac M, et al. Lipid phosphatase SHIP-1 regulates chondrocyte hypertrophy and skeletal development. J Cell Physiol. 2019;: pubmed publisher
Legrand J, Chan A, La H, Rossello F, Ankö M, Fuller Pace F, et al. DDX5 plays essential transcriptional and post-transcriptional roles in the maintenance and function of spermatogonia. Nat Commun. 2019;10:2278 pubmed publisher
Agarwal P, Isringhausen S, Li H, Paterson A, He J, Gomariz Á, et al. Mesenchymal Niche-Specific Expression of Cxcl12 Controls Quiescence of Treatment-Resistant Leukemia Stem Cells. Cell Stem Cell. 2019;24:769-784.e6 pubmed publisher
Kidoya H, Muramatsu F, Shimamura T, Jia W, Satoh T, Hayashi Y, et al. Regnase-1-mediated post-transcriptional regulation is essential for hematopoietic stem and progenitor cell homeostasis. Nat Commun. 2019;10:1072 pubmed publisher
Korski K, Kubli D, Wang B, Khalafalla F, Monsanto M, Firouzi F, et al. Hypoxia Prevents Mitochondrial Dysfunction and Senescence in Human c-Kit+ Cardiac Progenitor Cells. Stem Cells. 2019;37:555-567 pubmed publisher
Xie D, Liao Y, Wu B, Chen Y, Lin W, Lu D, et al. Cardiac Nestin+ Cells Derived from Early Stage of Dilated Cardiomyopathy Enhanced the Survival of the Doxorubicin-Injured Cardiac Muscle HL-1 Cells. Int Heart J. 2018;59:180-189 pubmed publisher
Hosseini Z, Nelson D, Moskwa N, Sfakis L, Castracane J, Larsen M. FGF2-dependent mesenchyme and laminin-111 are niche factors in salivary gland organoids. J Cell Sci. 2018;131: pubmed publisher
Saeidi S, Shapouri F, de Iongh R, Casagranda F, Sutherland J, Western P, et al. Esrp1 is a marker of mouse fetal germ cells and differentially expressed during spermatogenesis. PLoS ONE. 2018;13:e0190925 pubmed publisher
Coutu D, Kokkaliaris K, Kunz L, Schroeder T. Multicolor quantitative confocal imaging cytometry. Nat Methods. 2018;15:39-46 pubmed publisher
Kawai Y, Oda A, Kanai Y, Goitsuka R. Germ cell-intrinsic requirement for the homeodomain transcription factor PKnox1/Prep1 in adult spermatogenesis. PLoS ONE. 2018;13:e0190702 pubmed publisher
Harrell J, Shroka T, Jacobsen B. Estrogen induces c-Kit and an aggressive phenotype in a model of invasive lobular breast cancer. Oncogenesis. 2017;6:396 pubmed publisher
Magadum A, Ding Y, He L, Kim T, Vasudevarao M, Long Q, et al. Live cell screening platform identifies PPARδ as a regulator of cardiomyocyte proliferation and cardiac repair. Cell Res. 2017;27:1002-1019 pubmed publisher
Carrieri C, Comazzetto S, Grover A, Morgan M, Buness A, Nerlov C, et al. A transit-amplifying population underpins the efficient regenerative capacity of the testis. J Exp Med. 2017;214:1631-1641 pubmed publisher
Monsanto M, White K, Kim T, Wang B, Fisher K, Ilves K, et al. Concurrent Isolation of 3 Distinct Cardiac Stem Cell Populations From a Single Human Heart Biopsy. Circ Res. 2017;121:113-124 pubmed publisher
Lee M, Ha S, Park C, Park P, Fuchs R, Wei L, et al. Transcriptome of interstitial cells of Cajal reveals unique and selective gene signatures. PLoS ONE. 2017;12:e0176031 pubmed publisher
Luo L, Tang J, Nishi K, Yan C, Dinh P, Cores J, et al. Fabrication of Synthetic Mesenchymal Stem Cells for the Treatment of Acute Myocardial Infarction in Mice. Circ Res. 2017;120:1768-1775 pubmed publisher
Feng A, Xiang Y, Gui L, Kaltsidis G, Feng Q, Lu W. Paracrine GABA and insulin regulate pancreatic alpha cell proliferation in a mouse model of type 1 diabetes. Diabetologia. 2017;60:1033-1042 pubmed publisher
Luo L, Urata Y, Yan C, Hasan A, Goto S, Guo C, et al. Radiation Exposure Decreases the Quantity and Quality of Cardiac Stem Cells in Mice. PLoS ONE. 2016;11:e0152179 pubmed publisher
Liu Q, Yang R, Huang X, Zhang H, He L, Zhang L, et al. Genetic lineage tracing identifies in situ Kit-expressing cardiomyocytes. Cell Res. 2016;26:119-30 pubmed publisher
product information
brand :
R&D Systems
master code :
AF1356
SKU :
AF1356
product name :
Human/Mouse CD117/c-kit Antibody
unit size :
100 ug (also 25 ug)
seo description :
The Human/Mouse CD117/c-kit Antibody from R&D Systems is a goat polyclonal antibody to CD117/c-kit. This antibody reacts with human,mouse,porcine,rat,transgenic mouse. The Human/Mouse CD117/c-kit Antibody has been validated for the following applications: Immunohistochemistry,Western Blot,Flow Cytometry,Simple Western,Neutralization,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunofluorescence,Immunocytochemistry,Immunocytochemistry/ Immunofluorescence,CyTOF-ready,Dual RNAscope ISH-IHC Compatible.
target :
CD117/c-kit
category :
Primary Antibodies
buffer :
Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.
clonality :
Polyclonal
concentration :
LYOPH
conjugate :
Unconjugated
dilution :
Western Blot 0.1 ug/mL, Simple Western 5 ug/mL, Flow Cytometry 2.5 ug/10^6 cells, Immunohistochemistry 5-15 ug/mL, CyTOF-ready, Dual RNAscope ISH-IHC 5-15 ug/mL
host :
Goat
immunogen :
Mouse myeloma cell line NS0-derived recombinant mouse CD117/c-kit, Gln25-Thr519 (Ala207Glu), Accession # P05532
isotype :
IgG
purity :
Antigen Affinity-purified
species :
Human,Mouse,Porcine,Rat,Transgenic Mouse
specificity :
Detects human and mouse CD117/c-kit in direct ELISAs and Western blots.
gene symbol :
KIT
top caption :
CD117/c-kit antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr).
accessionNumbers :
P05532
applications :
CyTOF-ready,Dual RNAscope ISH-IHC Compatible,Flow Cytometry,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Simple Western,Neutralization,Immunofluorescence,Immunocytochemistry,Immunocytochemistry/ Immunofluorescence
USD :
499 USD
alt names :
CD117, CD117 antigen, ckit, C-Kit, EC 2.7.10, EC 2.7.10.1, KIT, PBT, piebald trait, Proto-oncogene c-Kit, proto-oncogene tyrosine-protein kinase Kit, SCF R, SCFR, SCFRmast/stem cell growth factor receptor, soluble KIT variant 1, Tyrosine-protein kinase Kit, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.
more info or order :
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.