product summary
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company name :
R&D Systems
product type :
antibody
product name :
Human/Mouse/Rat/Canine ALCAM/CD166 Antibody
catalog :
AF1172
quantity :
100 ug (also 25 ug)
price :
499 USD
clonality :
polyclonal
host :
domestic goat
conjugate :
nonconjugated
reactivity :
human, mouse, rat, dogs
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, immunohistochemistry knockout validation
more info or order :
R&D Systems product webpage
citations: 12
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry knockout validation; mouse; 1:50; loading ...; fig s1c
Delgado C, Bu L, Zhang J, Liu F, Sall J, Liang F, et al. Neural cell adhesion molecule is required for ventricular conduction system development. Development. 2021;148: pubmed publisher
Mapunda J, Pareja J, Vladymyrov M, Bouillet E, H xe9 lie P, Pleska x10d P, et al. VE-cadherin in arachnoid and pia mater cells serves as a suitable landmark for in vivo imaging of CNS immune surveillance and inflammation. Nat Commun. 2023;14:5837 pubmed publisher
Yeo R, Zhou O, Zhong B, Sun E, Navarro Negredo P, Nair S, et al. Chromatin accessibility dynamics of neurogenic niche cells reveal defects in neural stem cell adhesion and migration during aging. Nat Aging. 2023;3:866-893 pubmed publisher
He S, Li S, Guo J, Zeng X, Liang D, Zhu Y, et al. CD166-specific CAR-T cells potently target colorectal cancer cells. Transl Oncol. 2023;27:101575 pubmed publisher
Yamashita H, Fukuda K, Hattori F. Hepatocyte-like Cells Derived from Human Pluripotent Stem Cells Can Be Enriched by a Combination of Mitochondrial Content and Activated Leukocyte Cell Adhesion Molecule. JMA J. 2019;2:174-183 pubmed publisher
Ng A, Khoshakhlagh P, Rojo Arias J, Pasquini G, Wang K, Swiersy A, et al. A comprehensive library of human transcription factors for cell fate engineering. Nat Biotechnol. 2020;: pubmed publisher
Iwagishi R, Tanaka R, Seto M, Takagi T, Norioka N, Ueyama T, et al. Negatively charged amino acids in the stalk region of membrane proteins reduce ectodomain shedding. J Biol Chem. 2020;295:12343-12352 pubmed publisher
Renard H, Tyckaert F, Lo Giudice C, Hirsch T, Valades Cruz C, Lemaigre C, et al. Endophilin-A3 and Galectin-8 control the clathrin-independent endocytosis of CD166. Nat Commun. 2020;11:1457 pubmed publisher
Bye C, Rytova V, Alsanie W, Parish C, Thompson L. Axonal Growth of Midbrain Dopamine Neurons is Modulated by the Cell Adhesion Molecule ALCAM Through Trans-Heterophilic Interactions with L1cam, Chl1, and Semaphorins. J Neurosci. 2019;39:6656-6667 pubmed publisher
Liu J, Reggiani J, Laboulaye M, Pandey S, Chen B, Rubenstein J, et al. Tbr1 instructs laminar patterning of retinal ganglion cell dendrites. Nat Neurosci. 2018;21:659-670 pubmed publisher
Coutu D, Kokkaliaris K, Kunz L, Schroeder T. Multicolor quantitative confocal imaging cytometry. Nat Methods. 2018;15:39-46 pubmed publisher
Lécuyer M, Saint Laurent O, Bourbonnière L, Larouche S, Larochelle C, Michel L, et al. Dual role of ALCAM in neuroinflammation and blood-brain barrier homeostasis. Proc Natl Acad Sci U S A. 2017;114:E524-E533 pubmed publisher
product information
brand :
R&D Systems
master code :
AF1172
SKU :
AF1172
product name :
Human/Mouse/Rat/Canine ALCAM/CD166 Antibody
unit size :
100 ug (also 25 ug)
seo description :
The Human/Mouse/Rat/Canine ALCAM/CD166 Antibody from R&D Systems is a goat polyclonal antibody to ALCAM/CD166. This antibody reacts with canine,human,mouse,rat. The Human/Mouse/Rat/Canine ALCAM/CD166 Antibody has been validated for the following applications: Immunocytochemistry,Flow Cytometry,Immunoprecipitation,Western Blot,Immunohistochemistry,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunofluorescence,Simple Western,CyTOF-ready.
target :
ALCAM/CD166
category :
Primary Antibodies
buffer :
Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.
clonality :
Polyclonal
concentration :
LYOPH
conjugate :
Unconjugated
dilution :
Western Blot 0.2 ug/mL, Simple Western 12.5 ug/mL, Flow Cytometry 0.25 ug/10^6 cells, Immunohistochemistry 5-15 ug/mL, Immunocytochemistry 5-15 ug/mL, CyTOF-ready
host :
Goat
immunogen :
Mouse myeloma cell line NS0-derived recombinant mouse ALCAM/CD166, Trp28-Lys527, Accession # AAC06342
isotype :
IgG
purity :
Antigen Affinity-purified
species :
Canine,Human,Mouse,Rat
specificity :
Detects human, mouse and rat ALCAM/CD166 in Western blots. In direct ELISAs, less than 10% cross-reactivity with rhBCAM, recombinant mouse (rm) OCAM, and rmMAdCAM-1 is observed. Detects canine ALCAM/CD166 in flow cytometry.
gene symbol :
ALCAM
top caption :
Detection of Human, Mouse, and Rat ALCAM/CD166 antibody by Western Blot.
accessionNumbers :
AAC06342
applications :
Simple Western,CyTOF-ready,Flow Cytometry,Immunohistochemistry,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunofluorescence,Immunocytochemistry
USD :
499 USD
alt names :
activated leucocyte cell adhesion molecule, activated leukocyte cell adhesion moleculeFLJ38514, CD166, CD166 antigen, MEMDMGC71733
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.
more info or order :
R&D Systems product webpage
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.