Human PD-1 Antibody | R&D Systems AF1086 product information

product summary

company name :

R&D Systems

product type :

antibody

product name :

Human PD-1 Antibody

catalog :

AF1086

quantity :

100 ug (also 25 ug)

price :

499 USD

clonality :

polyclonal

host :

domestic goat

conjugate :

nonconjugated

reactivity :

human, mouse, rhesus macaque

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, neutralization, flow cytometry, immunohistochemistry - paraffin section

more info or order :

R&D Systems product webpage

citations: 37

Reference
H xe4 nze J, Schulte Herbr xfc ggen J, Hofmann R, Hegele A. Modulation of immune checkpoint regulators in interferon γ induced urothelial carcinoma and activated T-lymphocyte cells by cytostatics. Genes Immun. 2023;24:149-153 pubmed publisher
Wu J, Zhu R, Wang Z, Chen X, Xu T, Liu Y, et al. Exosomes in malignant pleural effusion from lung cancer patients impaired the cytotoxicity of double-negative T cells. Transl Oncol. 2023;27:101564 pubmed publisher
Guo Q, Shen S, Guan G, Zhu C, Zou C, Cao J, et al. Cancer cell intrinsic TIM-3 induces glioblastoma progression. iScience. 2022;25:105329 pubmed publisher
Goubet A, Lordello L, Alves Costa Silva C, Peguillet I, Gazzano M, Mbogning Fonkou M, et al. Escherichia coli-Specific CXCL13-Producing TFH Are Associated with Clinical Efficacy of Neoadjuvant PD-1 Blockade against Muscle-Invasive Bladder Cancer. Cancer Discov. 2022;12:2280-2307 pubmed publisher
Shankar S, Stolp J, Juvet S, Beckett J, Macklin P, Issa F, et al. Ex vivo-expanded human CD19+TIM-1+ regulatory B cells suppress immune responses in vivo and are dependent upon the TIM-1/STAT3 axis. Nat Commun. 2022;13:3121 pubmed publisher
Gan F, Zhang C, Xia L, Deng S. Tumor-endogenous PD-1 promotes cell proliferation and predicts poor survival in non-small cell lung cancer. Transl Cancer Res. 2022;11:3-13 pubmed publisher
Keber C, Derigs M, Schultz C, Wegner M, Lingelbach S, Wischmann V, et al. Cellular and soluble immune checkpoint signaling forms PD-L1 and PD-1 in renal tumor tissue and in blood. Cancer Immunol Immunother. 2022;71:2381-2389 pubmed publisher
Rafei Shamsabadi D, Lehr S, Behrens M, Meiss F. Additive Intralesional Interleukin-2 Improves Progression-Free Survival in a Distinct Subgroup of Melanoma Patients with Prior Progression under Immunotherapy. Cancers (Basel). 2022;14: pubmed publisher
Cheng B, Ding K, Chen P, Ji J, Luo T, Guo X, et al. Anti-PD-L1/TGF-βR fusion protein (SHR-1701) overcomes disrupted lymphocyte recovery-induced resistance to PD-1/PD-L1 inhibitors in lung cancer. Cancer Commun (Lond). 2022;42:17-36 pubmed publisher
von Schoenfeld A, Bronsert P, Poc M, Fuller A, Filby A, Kraft S, et al. Multiple Immunostainings with Different Epitope Retrievals-The FOLGAS Protocol. Int J Mol Sci. 2021;23: pubmed publisher
Takasu C, Yamashita S, Morine Y, Yoshikawa K, Tokunaga T, Nishi M, et al. The role of the immunoescape in colorectal cancer liver metastasis. PLoS ONE. 2021;16:e0259940 pubmed publisher
Sarkar S, Spencer D, Barnette P, Pandey S, Sutton W, Basu M, et al. CD4+ T Cells Are Dispensable for Induction of Broad Heterologous HIV Neutralizing Antibodies in Rhesus Macaques. Front Immunol. 2021;12:757811 pubmed publisher
Weber M, Walters Laird C, Kol A, Santos Rocha C, Hirao L, Mende A, et al. Gut germinal center regeneration and enhanced antiviral immunity by mesenchymal stem/stromal cells in SIV infection. JCI Insight. 2021;6: pubmed publisher
Glorieux C, Xia X, He Y, Hu Y, Cremer K, Robert A, et al. Regulation of PD-L1 expression in K-ras-driven cancers through ROS-mediated FGFR1 signaling. Redox Biol. 2021;38:101780 pubmed publisher
Takasu C, Nishi M, Yoshikawa K, Tokunaga T, Kashihara H, Yoshimoto T, et al. Impact of sidedness of colorectal cancer on tumor immunity. PLoS ONE. 2020;15:e0240408 pubmed publisher
Deng R, Zuo C, Li Y, Xue B, Xun Z, Guo Y, et al. The innate immune effector ISG12a promotes cancer immunity by suppressing the canonical Wnt/β-catenin signaling pathway. Cell Mol Immunol. 2020;17:1163-1179 pubmed publisher
Gesser B, Rasmussen M, Iversen L. Dimethyl Fumarate Targets MSK1, RSK1, 2 and IKKα/β Kinases and Regulates NF-κB /p65 Activation in Psoriasis: A Demonstration of the Effect on Peripheral Blood Mononuclear Cells, Drawn from Two Patients with Severe Psoriasis Before a. Psoriasis (Auckl). 2020;10:1-11 pubmed publisher
Bucur O, Fu F, Calderon M, Mylvaganam G, Ly N, Day J, et al. Nanoscale imaging of clinical specimens using conventional and rapid-expansion pathology. Nat Protoc. 2020;15:1649-1672 pubmed publisher
Eckel Passow J, Ho T, Serie D, Cheville J, Houston Thompson R, Costello B, et al. Concordance of PD-1 and PD-L1 (B7-H1) in paired primary and metastatic clear cell renal cell carcinoma. Cancer Med. 2020;9:1152-1160 pubmed publisher
Baruah P, Bullenkamp J, Wilson P, Lee M, Kaski J, Dumitriu I. TLR9 Mediated Tumor-Stroma Interactions in Human Papilloma Virus (HPV)-Positive Head and Neck Squamous Cell Carcinoma Up-Regulate PD-L1 and PD-L2. Front Immunol. 2019;10:1644 pubmed publisher
Yang S, Wei J, Cui Y, Park G, Shah P, Deng Y, et al. m6A mRNA demethylase FTO regulates melanoma tumorigenicity and response to anti-PD-1 blockade. Nat Commun. 2019;10:2782 pubmed publisher
Taechangam N, Iyer S, Walker N, Arzi B, Borjesson D. Mechanisms utilized by feline adipose-derived mesenchymal stem cells to inhibit T lymphocyte proliferation. Stem Cell Res Ther. 2019;10:188 pubmed publisher
Li S, Folkvord J, Kovács K, Wagstaff R, Mwakalundwa G, Rendahl A, et al. Low levels of SIV-specific CD8+ T cells in germinal centers characterizes acute SIV infection. PLoS Pathog. 2019;15:e1007311 pubmed publisher
Li B, SONG T, Wang F, Yin C, Li Z, Lin J, et al. Tumor-derived exosomal HMGB1 promotes esophageal squamous cell carcinoma progression through inducing PD1+ TAM expansion. Oncogenesis. 2019;8:17 pubmed publisher
Sampedro Nuñez M, Serrano Somavilla A, Adrados M, Cameselle Teijeiro J, Blanco Carrera C, Cabezas Agricola J, et al. Analysis of expression of the PD-1/PD-L1 immune checkpoint system and its prognostic impact in gastroenteropancreatic neuroendocrine tumors. Sci Rep. 2018;8:17812 pubmed publisher
Wang F, Li B, Wei Y, Zhao Y, Wang L, Zhang P, et al. Tumor-derived exosomes induce PD1+ macrophage population in human gastric cancer that promotes disease progression. Oncogenesis. 2018;7:41 pubmed publisher
Du S, McCall N, Park K, Guan Q, Fontina P, Ertel A, et al. Blockade of Tumor-Expressed PD-1 promotes lung cancer growth. Oncoimmunology. 2018;7:e1408747 pubmed publisher
Fan W, Demers A, Wan Y, Li Q. Altered Ratio of T Follicular Helper Cells to T Follicular Regulatory Cells Correlates with Autoreactive Antibody Response in Simian Immunodeficiency Virus-Infected Rhesus Macaques. J Immunol. 2018;200:3180-3187 pubmed publisher
Rahman M, McKinnon K, Karpova T, Ball D, Venzon D, Fan W, et al. Associations of Simian Immunodeficiency Virus (SIV)-Specific Follicular CD8+ T Cells with Other Follicular T Cells Suggest Complex Contributions to SIV Viremia Control. J Immunol. 2018;200:2714-2726 pubmed publisher
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Liu Y, Cheng Y, Xu Y, Wang Z, Du X, Li C, et al. Increased expression of programmed cell death protein 1 on NK cells inhibits NK-cell-mediated anti-tumor function and indicates poor prognosis in digestive cancers. Oncogene. 2017;36:6143-6153 pubmed publisher
Silveira E, Rogers K, Gumber S, Amancha P, Xiao P, Woollard S, et al. Immune Cell Dynamics in Rhesus Macaques Infected with a Brazilian Strain of Zika Virus. J Immunol. 2017;199:1003-1011 pubmed publisher
Martinez Murillo P, Tran K, Guenaga J, Lindgren G, Adori M, Feng Y, et al. Particulate Array of Well-Ordered HIV Clade C Env Trimers Elicits Neutralizing Antibodies that Display a Unique V2 Cap Approach. Immunity. 2017;46:804-817.e7 pubmed publisher
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Obeid J, Erdag G, Smolkin M, Deacon D, Patterson J, Chen L, et al. PD-L1, PD-L2 and PD-1 expression in metastatic melanoma: Correlation with tumor-infiltrating immune cells and clinical outcome. Oncoimmunology. 2016;5:e1235107 pubmed publisher
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product information

brand :

R&D Systems

master code :

AF1086

SKU :

AF1086

product name :

Human PD-1 Antibody

unit size :

100 ug (also 25 ug)

seo description :

The Human PD-1 Antibody from R&D Systems is a goat polyclonal antibody to PD-1. This antibody reacts with feline,human,mouse,primate,primate - cercocebus atys (sooty mangabey),primate - macaca mulatta (rhesus macaque). The Human PD-1 Antibody has been validated for the following applications: Immunohistochemistry,Bioassay,ELISA Development,Flow Cytometry,Western Blot,Neutralization,Functional Assay,Immunohistochemistry-Paraffin,Immunocytochemistry,Blockade of Receptor-ligand Interaction,ELISA Capture (Matched Antibody Pair),CyTOF-ready,Dual RNAscope ISH-IHC Compatible.

target :

PD-1

category :

Primary Antibodies

buffer :

Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.

clonality :

Polyclonal

concentration :

LYOPH

conjugate :

Unconjugated

dilution :

Western Blot 0.5-2 ug/mL, Flow Cytometry 0.25 ug/10^6 cells, Immunohistochemistry 5-15 ug/mL, Blockade of Receptor-ligand Interaction, CyTOF-ready, Dual RNAscope ISH-IHC 5-15 ug/mL, ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL

host :

Goat

immunogen :

Mouse myeloma cell line NS0-derived recombinant human PD-1, Leu25-Gln167, Accession # Q8IX89

isotype :

IgG

purity :

Antigen Affinity-purified

species :

Feline,Human,Mouse,Primate,Primate - Cercocebus atys (Sooty Mangabey),Primate - Macaca mulatta (Rhesus Macaque)

specificity :

Detects human PD-1 in ELISAs and Western blots. In sandwich ELISAs, less than 2% cross-reactivity with recombinant mouse PD-1 and less than 0.2% cross-reactivity with recombinant human (rh) CD28, rhICOS, and rhCTLA-4 is observed.

gene symbol :

PDCD1

endotoxin note :

<0.10 EU per 1 ╡g of the antibody by the LAL method.

top caption :

Detection of Human PD-1 antibody by Western Blot.

accessionNumbers :

Q8IX89

applications :

Blockade of Receptor-ligand Interaction,ELISA Capture (Matched Antibody Pair),CyTOF-ready,Dual RNAscope ISH-IHC Compatible,Bioassay,ELISA Development,Flow Cytometry,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Neutralization,Immunocytochemistry,Functional Assay

USD :

499 USD

alt names :

CD279, CD279 antigen, hPD-1, PD1, PD1hPD-l, PDCD1, programmed cell death 1, programmed cell death protein 1, Protein PD-1, SLEB2

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage