product summary
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company name :
R&D Systems
product type :
antibody
product name :
Mouse PD-L1/B7-H1 Antibody
catalog :
AF1019
quantity :
100 ug (also 25 ug)
price :
499 USD
clonality :
polyclonal
host :
domestic goat
conjugate :
nonconjugated
reactivity :
human, mouse
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
citations: 50
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - paraffin section; mouse; 1:100; fig s7f
Taniguchi H, Caeser R, Chavan S, Zhan Y, Chow A, Manoj P, et al. WEE1 inhibition enhances the antitumor immune response to PD-L1 blockade by the concomitant activation of STING and STAT1 pathways in SCLC. Cell Rep. 2022;39:110814 pubmed publisher
  • western blot; mouse; loading ...; fig 10p, 10q
Liu J, Wang C, Cheng T, Rixiati Y, Ji C, Deng M, et al. Circadian Clock Disruption Suppresses PDL1+ Intraepithelial B Cells in Experimental Colitis and Colitis-Associated Colorectal Cancer. Cell Mol Gastroenterol Hepatol. 2021;12:251-276 pubmed publisher
Wei C, Huang L, Kreh B, Liu X, Tyutyunyk Massey L, Kawakami M, et al. A novel retinoic acid receptor-γ agonist antagonizes immune checkpoint resistance in lung cancers by altering the tumor immune microenvironment. Sci Rep. 2023;13:14907 pubmed publisher
Rachdi L, Zhou Z, Berthault C, Louren xe7 o C, Fouque A, Domet T, et al. Tryptophan metabolism promotes immune evasion in human pancreatic β cells. EBioMedicine. 2023;95:104740 pubmed publisher
Zhang W, Pan X, Xu Y, Guo H, Zheng M, Chen X, et al. Mevalonate improves anti-PD-1/PD-L1 efficacy by stabilizing CD274 mRNA. Acta Pharm Sin B. 2023;13:2585-2600 pubmed publisher
Shrestha M, Wang D, Ben David Y, Zacksenhaus E. CDK4/6 inhibitors and the pRB-E2F1 axis suppress PVR and PD-L1 expression in triple-negative breast cancer. Oncogenesis. 2023;12:29 pubmed publisher
Tseng H, Banerjee S, Qian B, Lai M, Wu T, Hsu T, et al. Design, synthesis, and biological activity of dual monoamine oxidase A and heat shock protein 90 inhibitors, N-Methylpropargylamine-conjugated 4-isopropylresorcinol for glioblastoma. Eur J Med Chem. 2023;256:115459 pubmed publisher
Xu C, Xia Y, Zhang B, Drokow E, Li H, Xu S, et al. Macrophages facilitate tumor cell PD-L1 expression via an IL-1β-centered loop to attenuate immune checkpoint blockade. MedComm (2020). 2023;4:e242 pubmed publisher
Wang S, Kuai Y, Lin S, Li L, Gu Q, Zhang X, et al. NF-κB Activator 1 downregulation in macrophages activates STAT3 to promote adenoma-adenocarcinoma transition and immunosuppression in colorectal cancer. BMC Med. 2023;21:115 pubmed publisher
Sato M, Maishi N, Hida Y, Yanagawa Matsuda A, Alam M, Sakakibara Konishi J, et al. Angiogenic inhibitor pre-administration improves the therapeutic effects of immunotherapy. Cancer Med. 2023;12:9760-9773 pubmed publisher
De Burghgrave M, Louren xe7 o C, Berthault C, Aiello V, Villalba A, Fouque A, et al. Pancreatic Islet Cells Response to IFNγ Relies on Their Spatial Location within an Islet. Cells. 2022;12: pubmed publisher
Shahbandi A, Chiu F, Ungerleider N, Kvadas R, Mheidly Z, Sun M, et al. Breast cancer cells survive chemotherapy by activating targetable immune-modulatory programs characterized by PD-L1 or CD80. Nat Cancer. 2022;3:1513-1533 pubmed publisher
Mokkapati S, Narayan V, Manyam G, Lim A, Duplisea J, Kokorovic A, et al. Lentiviral interferon: A novel method for gene therapy in bladder cancer. Mol Ther Oncolytics. 2022;26:141-157 pubmed publisher
Tengesdal I, Li S, Powers N, May M, Neff C, Joosten L, et al. Activation of Host-NLRP3 Inflammasome in Myeloid Cells Dictates Response to Anti-PD-1 Therapy in Metastatic Breast Cancers. Pharmaceuticals (Basel). 2022;15: pubmed publisher
Li Y, Wu L, Liu Y, Ma S, Huang B, Feng X, et al. A novel multifunctional anti-PD-L1-CD16a-IL15 induces potent cancer cell killing in PD-L1-positive tumour cells. Transl Oncol. 2022;21:101424 pubmed publisher
Barley T, Murphy P, Wang X, Bowman B, Mormol J, Mager C, et al. Mitogen-activated protein kinase phosphatase-1 controls PD-L1 expression by regulating type I interferon during systemic Escherichia coli infection. J Biol Chem. 2022;298:101938 pubmed publisher
Pham T, Spaulding C, Shields M, Metropulos A, Shah D, Khalafalla M, et al. Inhibition of MNKs promotes macrophage immunosuppressive phenotype to limit CD8+ T cell antitumor immunity. JCI Insight. 2022;7: pubmed publisher
Sandker G, Adema G, Molkenboer Kuenen J, Wierstra P, Bussink J, Heskamp S, et al. PD-L1 Antibody Pharmacokinetics and Tumor Targeting in Mouse Models for Infectious Diseases. Front Immunol. 2022;13:837370 pubmed publisher
Shinchi Y, Ishizuka S, Komohara Y, Matsubara E, Mito R, Pan C, et al. The expression of PD-1 ligand 1 on macrophages and its clinical impacts and mechanisms in lung adenocarcinoma. Cancer Immunol Immunother. 2022;71:2645-2661 pubmed publisher
Jin J, Sivakumar I, Mironchik Y, Krishnamachary B, Wildes F, Barnett J, et al. PD-L1 near Infrared Photoimmunotherapy of Ovarian Cancer Model. Cancers (Basel). 2022;14: pubmed publisher
Hagemans I, Wierstra P, Steuten K, Molkenboer Kuenen J, van Dalen D, ter Beest M, et al. Multiscale imaging of therapeutic anti-PD-L1 antibody localization using molecularly defined imaging agents. J Nanobiotechnology. 2022;20:64 pubmed publisher
Synn C, Kim S, Lee H, Kim M, Kim J, Lee J, et al. SKI-G-801, an AXL kinase inhibitor, blocks metastasis through inducing anti-tumor immune responses and potentiates anti-PD-1 therapy in mouse cancer models. Clin Transl Immunology. 2022;11:e1364 pubmed publisher
Ishikura N, Sugimoto M, Yorozu K, Kurasawa M, Kondoh O. Anti‑VEGF antibody triggers the effect of anti‑PD‑L1 antibody in PD‑L1low and immune desert‑like mouse tumors. Oncol Rep. 2022;47: pubmed publisher
Shi Z, Mori Akiyama Y, Du W, Fultz R, Zhao Y, Ruan W, et al. Loss of H2R Signaling Disrupts Neutrophil Homeostasis and Promotes Inflammation-Associated Colonic Tumorigenesis in Mice. Cell Mol Gastroenterol Hepatol. 2022;13:717-737 pubmed publisher
Kim J, Jang H, Lee G, Hur Y, Keum J, Jo J, et al. A Novel Kinase Inhibitor AX-0085 Inhibits Interferon-γ-Mediated Induction of PD-L1 Expression and Promotes Immune Reaction to Lung Adenocarcinoma Cells. Cells. 2021;11: pubmed publisher
Morrissey S, Zhang F, Ding C, Montoya Durango D, Hu X, Yang C, et al. Tumor-derived exosomes drive immunosuppressive macrophages in a pre-metastatic niche through glycolytic dominant metabolic reprogramming. Cell Metab. 2021;33:2040-2058.e10 pubmed publisher
Lenehan P, Cirella A, Uchida A, Crowley S, Sharova T, Boland G, et al. Type 2 immunity is maintained during cancer-associated adipose tissue wasting. Immunother Adv. 2021;1:ltab011 pubmed publisher
El Meskini R, Atkinson D, Kulaga A, Abdelmaksoud A, Gumprecht M, Pate N, et al. Distinct Biomarker Profiles and TCR Sequence Diversity Characterize the Response to PD-L1 Blockade in a Mouse Melanoma Model. Mol Cancer Res. 2021;19:1422-1436 pubmed publisher
Kim S, Kim S, Nam G, Hong Y, Kim G, Choi Y, et al. In situ immunogenic clearance induced by a combination of photodynamic therapy and rho-kinase inhibition sensitizes immune checkpoint blockade response to elicit systemic antitumor immunity against intraocular melanoma and its metastasis. J Immunother Cancer. 2021;9: pubmed publisher
Fernandes R, Su L, Nishiga Y, Ren J, Bhuiyan A, Cheng N, et al. Immune receptor inhibition through enforced phosphatase recruitment. Nature. 2020;586:779-784 pubmed publisher
Lerbs T, Cui L, King M, Chai T, Muscat C, Chung L, et al. CD47 prevents the elimination of diseased fibroblasts in scleroderma. JCI Insight. 2020;5: pubmed publisher
Philippou Y, Sjoberg H, Murphy E, Alyacoubi S, Jones K, Gordon Weeks A, et al. Impacts of combining anti-PD-L1 immunotherapy and radiotherapy on the tumour immune microenvironment in a murine prostate cancer model. Br J Cancer. 2020;123:1089-1100 pubmed publisher
Shi Y, Fu Y, Zhang X, Zhao G, Yao Y, Guo Y, et al. Romidepsin (FK228) regulates the expression of the immune checkpoint ligand PD-L1 and suppresses cellular immune functions in colon cancer. Cancer Immunol Immunother. 2021;70:61-73 pubmed publisher
Mondello P, Tadros S, Teater M, Fontan L, Chang A, Jain N, et al. Selective Inhibition of HDAC3 Targets Synthetic Vulnerabilities and Activates Immune Surveillance in Lymphoma. Cancer Discov. 2020;10:440-459 pubmed publisher
Hong Y, Kim Y, Kim G, Nam G, Kim S, Park Y, et al. Degradation of tumour stromal hyaluronan by small extracellular vesicle-PH20 stimulates CD103+ dendritic cells and in combination with PD-L1 blockade boosts anti-tumour immunity. J Extracell Vesicles. 2019;8:1670893 pubmed publisher
Schreiber L, Urbiola C, Das K, Spiesschaert B, Kimpel J, Heinemann F, et al. The lytic activity of VSV-GP treatment dominates the therapeutic effects in a syngeneic model of lung cancer. Br J Cancer. 2019;121:647-658 pubmed publisher
Burrack A, Spartz E, Raynor J, Wang I, Olson M, Stromnes I. Combination PD-1 and PD-L1 Blockade Promotes Durable Neoantigen-Specific T Cell-Mediated Immunity in Pancreatic Ductal Adenocarcinoma. Cell Rep. 2019;28:2140-2155.e6 pubmed publisher
Ritzmann F, Jungnickel C, Vella G, Kamyschnikow A, Herr C, Li D, et al. IL-17C-mediated innate inflammation decreases the response to PD-1 blockade in a model of Kras-driven lung cancer. Sci Rep. 2019;9:10353 pubmed publisher
Huang C, Tang X, Li S, Wang Q, Xie B, Xu J, et al. Immunopotentiator Aikejia improves the therapeutic efficacy of PD-1/PD-L1 immunosuppressive pathway in CT26.WT cancer cell. J Cancer. 2019;10:3472-3480 pubmed publisher
Kataru R, Ly C, Shin J, Park H, Baik J, Rehal S, et al. Tumor lymphatic function regulates tumor inflammatory and immunosuppressive microenvironments. Cancer Immunol Res. 2019;: pubmed publisher
Jagoda E, Vasalatiy O, Basuli F, Opina A, Williams M, Wong K, et al. Immuno-PET Imaging of the Programmed Cell Death-1 Ligand (PD-L1) Using a Zirconium-89 Labeled Therapeutic Antibody, Avelumab. Mol Imaging. 2019;18:1536012119829986 pubmed publisher
Yahata T, Mizoguchi M, Kimura A, Orimo T, Toujima S, Kuninaka Y, et al. Programmed cell death ligand 1 disruption by clustered regularly interspaced short palindromic repeats/Cas9-genome editing promotes antitumor immunity and suppresses ovarian cancer progression. Cancer Sci. 2019;110:1279-1292 pubmed publisher
Bartee M, Dryja P, Bartee E. Chimeric tumor modeling reveals role of partial PDL1 expression in resistance to virally induced immunotherapy. J Immunother Cancer. 2019;7:11 pubmed publisher
Osum K, Burrack A, Martinov T, Sahli N, Mitchell J, Tucker C, et al. Interferon-gamma drives programmed death-ligand 1 expression on islet β cells to limit T cell function during autoimmune diabetes. Sci Rep. 2018;8:8295 pubmed publisher
Srinivasan P, Wu X, Basu M, Rossi C, Sandler A. PD-L1 checkpoint inhibition and anti-CTLA-4 whole tumor cell vaccination counter adaptive immune resistance: A mouse neuroblastoma model that mimics human disease. PLoS Med. 2018;15:e1002497 pubmed publisher
Dimitrov V, Bouttier M, Boukhaled G, Salehi Tabar R, Avramescu R, Memari B, et al. Hormonal vitamin D up-regulates tissue-specific PD-L1 and PD-L2 surface glycoprotein expression in humans but not mice. J Biol Chem. 2017;292:20657-20668 pubmed publisher
Bartee M, Dunlap K, Bartee E. Tumor-Localized Secretion of Soluble PD1 Enhances Oncolytic Virotherapy. Cancer Res. 2017;77:2952-2963 pubmed publisher
Sung S, Ge Y, Dai C, Wang H, Fu S, Sharma R, et al. Dependence of Glomerulonephritis Induction on Novel Intraglomerular Alternatively Activated Bone Marrow-Derived Macrophages and Mac-1 and PD-L1 in Lupus-Prone NZM2328 Mice. J Immunol. 2017;198:2589-2601 pubmed publisher
Mascia F, Schloemann D, Cataisson C, McKinnon K, Krymskaya L, Wolcott K, et al. Cell autonomous or systemic EGFR blockade alters the immune-environment in squamous cell carcinomas. Int J Cancer. 2016;139:2593-7 pubmed publisher
Seifert A, Zeng S, Zhang J, Kim T, Cohen N, Beckman M, et al. PD-1/PD-L1 Blockade Enhances T-cell Activity and Antitumor Efficacy of Imatinib in Gastrointestinal Stromal Tumors. Clin Cancer Res. 2017;23:454-465 pubmed publisher
product information
brand :
R&D Systems
master code :
AF1019
SKU :
AF1019
product name :
Mouse PD-L1/B7-H1 Antibody
unit size :
100 ug (also 25 ug)
seo description :
The Mouse PD-L1/B7-H1 Antibody from R&D Systems is a goat polyclonal antibody to PD-L1. This antibody reacts with human,mouse. The Mouse PD-L1/B7-H1 Antibody has been validated for the following applications: Western Blot,Flow Cytometry,ELISA,Immunohistochemistry,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunocytochemistry,CyTOF-ready,Dual RNAscope ISH-IHC Compatible.
target :
PD-L1/B7-H1
category :
Primary Antibodies
buffer :
Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.
clonality :
Polyclonal
concentration :
LYOPH
conjugate :
Unconjugated
dilution :
Western Blot 0.5 ug/mL, Flow Cytometry 0.25 ug/10^6 cells, Immunohistochemistry 5-15 ug/mL, CyTOF-ready, Dual RNAscope ISH-IHC 5-15 ug/mL
host :
Goat
immunogen :
Mouse myeloma cell line NS0-derived recombinant mouse PD-L1/B7-H1, Phe19-Thr238, Accession # Q9EP73
isotype :
IgG
purity :
Antigen Affinity-purified
species :
Human,Mouse
specificity :
Detects mouse PD-L1/B7-H1 in direct ELISAs and Western blots.
gene symbol :
CD274
top caption :
Detection of Mouse PD-L1/B7-H1 antibody by Western Blot.
accessionNumbers :
Q9EP73
applications :
CyTOF-ready,Dual RNAscope ISH-IHC Compatible,Flow Cytometry,ELISA,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunocytochemistry
USD :
499 USD
alt names :
Avelumab, B7-H, B7H1, B7-H1, B7H1PDCD1L1, CD274, CD274 antigenMGC142294, CD274 molecule, PDCD1L1, PDCD1LG1, PDCD1LG1MGC142296, PDL1, PD-L1, PD-L1B7 homolog 1, PDL1PDCD1 ligand 1, programmed cell death 1 ligand 1, Programmed death ligand 1
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.
more info or order :
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.