Rat IL-1▀/IL-1F2 Antibody | R&D Systems AF-501-NA product information

product summary

company name :

R&D Systems

product type :

antibody

product name :

Rat IL-1▀/IL-1F2 Antibody

catalog :

AF-501-NA

quantity :

100 ug (also 25 ug)

price :

499 USD

clonality :

polyclonal

host :

domestic goat

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, neutralization, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, immunohistochemistry - free floating section

more info or order :

R&D Systems product webpage

citations: 36

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - free floating section; rat; 1:500; loading ...; fig 4f	Fern xe1 ndez Arjona M, Le xf3 n Rodr xed guez A, Grondona J, L xf3 pez xc1 valos M. Long-term priming of hypothalamic microglia is associated with energy balance disturbances under diet-induced obesity. Glia. 2022;70:1734-1761 pubmed publisher
	Sancho Alonso M, Arenas Y, Izquierdo Altarejos P, Martinez Garcia M, Llansola M, Felipo V. Enhanced Activation of the S1PR2-IL-1β-Src-BDNF-TrkB Pathway Mediates Neuroinflammation in the Hippocampus and Cognitive Impairment in Hyperammonemic Rats. Int J Mol Sci. 2023;24: pubmed publisher
	Koo Y, Lim C, Darai A, Lee J, Kim W, Han I, et al. Shape-memory collagen scaffold combined with hyaluronic acid for repairing intervertebral disc. Biomater Res. 2023;27:26 pubmed publisher
	Bhujel B, Yang S, Kim H, Kim S, Min B, Choi B, et al. An Injectable Engineered Cartilage Gel Improves Intervertebral Disc Repair in a Rat Nucleotomy Model. Int J Mol Sci. 2023;24: pubmed publisher
	Ikebukuro T, Arima T, Kasamatsu M, Nakano Y, Tobita Y, Uchiyama M, et al. Disulfiram Ophthalmic Solution Inhibited Macrophage Infiltration by Suppressing Macrophage Pseudopodia Formation in a Rat Corneal Alkali Burn Model. Int J Mol Sci. 2023;24: pubmed publisher
	Nagayach A, Bhaskar R, Patro I. Microglia activation and inflammation in hippocampus attenuates memory and mood functions during experimentally induced diabetes in rat. J Chem Neuroanat. 2022;125:102160 pubmed publisher
	Stekic A, Zeljkovic M, Zaric Kontic M, Mihajlovic K, Adzic M, Stevanovic I, et al. Intermittent Theta Burst Stimulation Ameliorates Cognitive Deficit and Attenuates Neuroinflammation via PI3K/Akt/mTOR Signaling Pathway in Alzheimer's-Like Disease Model. Front Aging Neurosci. 2022;14:889983 pubmed publisher
	Xu T, Li X, Guo Y, Uhlin E, Holmberg L, Mitra S, et al. Multiple therapeutic effects of human neural stem cells derived from induced pluripotent stem cells in a rat model of post-traumatic syringomyelia. EBioMedicine. 2022;77:103882 pubmed publisher
	Jiang M, Xie H, Zhang C, Wang T, Tian H, Lu L, et al. Enhancing fractalkine/CX3CR1 signalling pathway can reduce neuroinflammation by attenuating microglia activation in experimental diabetic retinopathy. J Cell Mol Med. 2022;26:1229-1244 pubmed publisher
	Wang Z, Xia Y, Wu Z, Kang S, Zhang J, Liu P, et al. Neuronal ApoE4 stimulates C/EBPβ activation, promoting Alzheimer's disease pathology in a mouse model. Prog Neurobiol. 2022;209:102212 pubmed publisher
	Dragi x107 M, Mitrovi x107 N, Ad x17e i x107 M, Nedeljkovi x107 N, Grkovi x107 I. Microglial- and Astrocyte-Specific Expression of Purinergic Signaling Components and Inflammatory Mediators in the Rat Hippocampus During Trimethyltin-Induced Neurodegeneration. ASN Neuro. 2021;13:17590914211044882 pubmed publisher
	Finney C, Shvetcov A, Westbrook R, Morris M, Jones N. The selective estrogen receptor modulator tamoxifen protects against subtle cognitive decline and early markers of injury 24 h after hippocampal silent infarct in male Sprague-Dawley rats. Horm Behav. 2021;134:105016 pubmed publisher
	Dragi x107 M, Zeljkovi x107 M, Stevanovi x107 I, Ad x17e i x107 M, Steki x107 A, Mihajlovi x107 K, et al. Downregulation of CD73/A2AR-Mediated Adenosine Signaling as a Potential Mechanism of Neuroprotective Effects of Theta-Burst Transcranial Magnetic Stimulation in Acute Experimental Autoimmune Encephalomyelitis. Brain Sci. 2021;11: pubmed publisher
	Fern xe1 ndez Arjona M, Le xf3 n Rodr xed guez A, L xf3 pez xc1 valos M, Grondona J. Microglia activated by microbial neuraminidase contributes to ependymal cell death. Fluids Barriers CNS. 2021;18:15 pubmed publisher
	Liu Y, Fu H, Wu Y, Nie B, Liu F, Wang T, et al. Elamipretide (SS-31) Improves Functional Connectivity in Hippocampus and Other Related Regions Following Prolonged Neuroinflammation Induced by Lipopolysaccharide in Aged Rats. Front Aging Neurosci. 2021;13:600484 pubmed publisher
	Wang Z, Xia Y, Liu P, Liu X, Edgington Mitchell L, Lei K, et al. ApoE4 activates C/EBPβ/δ-secretase with 27-hydroxycholesterol, driving the pathogenesis of Alzheimer's disease. Prog Neurobiol. 2021;202:102032 pubmed publisher
	Yamashita T, Kamikaseda S, Tanaka A, Tozaki Saitoh H, Caaveiro J, Inoue K, et al. New Inhibitory Effects of Cilnidipine on Microglial P2X7 Receptors and IL-1β Release: An Involvement in its Alleviating Effect on Neuropathic Pain. Cells. 2021;10: pubmed publisher
	Chen Z, Xie X, Jiang N, Li J, Shen L, Zhang Y. CCR5 signaling promotes lipopolysaccharide-induced macrophage recruitment and alveolar developmental arrest. Cell Death Dis. 2021;12:184 pubmed publisher
	Rusbana T, Agista A, Saputra W, Ohsaki Y, Watanabe K, Ardiansyah A, et al. Supplementation with Fermented Rice Bran Attenuates Muscle Atrophy in a Diabetic Rat Model. Nutrients. 2020;12: pubmed publisher
	Frank M, Baratta M, Zhang K, Fallon I, Pearson M, Liu G, et al. Acute stress induces the rapid and transient induction of caspase-1, gasdermin D and release of constitutive IL-1β protein in dorsal hippocampus. Brain Behav Immun. 2020;90:70-80 pubmed publisher
	Neves A, Farias F, de Magalh xe3 es S, Araldi D, Pagliusi M, Tambeli C, et al. Peripheral Inflammatory Hyperalgesia Depends on P2X7 Receptors in Satellite Glial Cells. Front Physiol. 2020;11:473 pubmed publisher
	Lambert W, Pasini S, Collyer J, Formichella C, Ghose P, Carlson B, et al. Of Mice and Monkeys: Neuroprotective Efficacy of the p38 Inhibitor BIRB 796 Depends on Model Duration in Experimental Glaucoma. Sci Rep. 2020;10:8535 pubmed publisher
	Fernández Arjona M, Grondona J, Fernández Llebrez P, López Ávalos M. Microglial activation by microbial neuraminidase through TLR2 and TLR4 receptors. J Neuroinflammation. 2019;16:245 pubmed publisher
	Yamamoto M, Kim M, Imai H, Itakura Y, Ohtsuki G. Microglia-Triggered Plasticity of Intrinsic Excitability Modulates Psychomotor Behaviors in Acute Cerebellar Inflammation. Cell Rep. 2019;28:2923-2938.e8 pubmed publisher
	Wang Z, Xiang J, Liu X, Yu S, Manfredsson F, Sandoval I, et al. Deficiency in BDNF/TrkB Neurotrophic Activity Stimulates δ-Secretase by Upregulating C/EBPβ in Alzheimer's Disease. Cell Rep. 2019;28:655-669.e5 pubmed publisher
	Aires I, Boia R, Rodrigues Neves A, Madeira M, Marques C, Ambrósio A, et al. Blockade of microglial adenosine A2A receptor suppresses elevated pressure-induced inflammation, oxidative stress, and cell death in retinal cells. Glia. 2019;67:896-914 pubmed publisher
	Lai H, Lu C, Wong C, Lin B, Chan S, Kuo C, et al. Baicalein attenuates neuropathic pain and improves sciatic nerve function recovery in rats with partial sciatic nerve transection. J Chin Med Assoc. 2018;81:955-963 pubmed publisher
	Jo C, Kim S, Park J, Kim G. Anti-Inflammatory Action of Sitagliptin and Linagliptin in Doxorubicin Nephropathy. Kidney Blood Press Res. 2018;43:987-999 pubmed publisher
	Zhang P, Bi R, Gan Y. Glial interleukin-1β upregulates neuronal sodium channel 1.7 in trigeminal ganglion contributing to temporomandibular joint inflammatory hypernociception in rats. J Neuroinflammation. 2018;15:117 pubmed publisher
	Taoro Gonzalez L, Arenas Y, Cabrera Pastor A, Felipo V. Hyperammonemia alters membrane expression of GluA1 and GluA2 subunits of AMPA receptors in hippocampus by enhancing activation of the IL-1 receptor: underlying mechanisms. J Neuroinflammation. 2018;15:36 pubmed publisher
	Wang H, Tsay H, Shih H, Chen Y, Chang K, Agrawal D, et al. Andrographolide relieved pathological pain generated by spared nerve injury model in mice. Pharm Biol. 2018;56:124-131 pubmed publisher
	Shih C, Hwang H, Chang C, Su H, Chen P, Kuo H, et al. Anti-Inflammatory and Antinociceptive Effects of Ethyl Acetate Fraction of an Edible Red Macroalgae Sarcodia ceylanica. Int J Mol Sci. 2017;18: pubmed publisher
	Natoli R, Fernando N, Madigan M, Chu Tan J, Valter K, Provis J, et al. Microglia-derived IL-1Î² promotes chemokine expression by MÃ¼ller cells and RPE in focal retinal degeneration. Mol Neurodegener. 2017;12:31 pubmed publisher
	Hong J, Li Y, Hua X, Bai Y, Wang C, Zhu C, et al. Lymphatic Circulation Disseminates Bartonella Infection Into Bloodstream. J Infect Dis. 2017;215:303-311 pubmed publisher
	García Pupo L, Zaldo Castro A, Exarchou V, Tacoronte Morales J, Pieters L, Vanden Berghe W, et al. In Vitro Neuroprotective and Anti-Inflammatory Activities of Natural and Semi-Synthetic Spirosteroid Analogues. Molecules. 2016;21: pubmed publisher
	Hernandez Rabaza V, Cabrera Pastor A, Taoro Gonzalez L, Gonzalez Usano A, Agusti A, Balzano T, et al. Neuroinflammation increases GABAergic tone and impairs cognitive and motor function in hyperammonemia by increasing GAT-3 membrane expression. Reversal by sulforaphane by promoting M2 polarization of microglia. J Neuroinflammation. 2016;13:83 pubmed publisher

product information

brand :

R&D Systems

master code :

AF-501-NA

SKU :

AF-501-NA

product name :

Rat IL-1▀/IL-1F2 Antibody

unit size :

100 ug (also 25 ug)

seo description :

The Rat IL-1ß/IL-1F2 Antibody from R&D Systems is a goat polyclonal antibody to IL-1 beta/IL-1F2. This antibody reacts with mouse,rat. The Rat IL-1ß/IL-1F2 Antibody has been validated for the following applications: ELISA Development,ELISA Development (Capture),Western Blot,Neutralization,Immunohistochemistry,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunocytochemistry,ELISA Capture (Matched Antibody Pair).

target :

IL-1 beta/IL-1F2

category :

Primary Antibodies

buffer :

Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.

clonality :

Polyclonal

concentration :

LYOPH

conjugate :

Unconjugated

dilution :

Western Blot 0.1 ug/mL, Neutralization 0.5-2.0 ug/mL, Immunocytochemistry 5-15 ug/mL, ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL

host :

Goat

immunogen :

E. coli -derived recombinant rat IL-1▀/IL-1F2 (R D Systems, Catalog # 501-RL), Val117-Ser268, Accession # Q63264

isotype :

IgG

purity :

Antigen Affinity-purified

species :

Mouse,Rat

specificity :

Detects rat IL-1▀/IL-1F2 in ELISAs and Western blots. In sandwich immunoassays, less than 1% cross-reactivity with recombinant mouse IL-1▀ is observed and less than 0.5% cross-reactivity with recombinant human IL-1▀ and recombinant porcine IL-1▀ is observed.

gene symbol :

IL1B

endotoxin note :

<0.10 EU per 1 ╡g of the antibody by the LAL method.

top caption :

Cell Proliferation Induced by IL-1ss/IL-1F2 and Neutralization by Rat IL-1ss/IL-1F2 Antibody.

accessionNumbers :

Q63264

applications :

ELISA Capture (Matched Antibody Pair),ELISA Development,ELISA Development (Capture),Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Neutralization,Immunocytochemistry

USD :

499 USD

alt names :

catabolin, IL-1, IL1 beta, IL-1 beta, IL-1b, IL1B, IL1-BETA, IL-1F2, IL1F2IL-1 beta, interleukin 1, beta, interleukin-1 beta, preinterleukin 1 beta, pro-interleukin-1-beta

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage