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company name :
R&D Systems
product type :
antibody
product name :
Mouse VEGF164 Antibody
catalog :
AF-493-NA
quantity :
100 ug (also 25 ug)
price :
519 USD
clonality :
polyclonal
host :
domestic goat
conjugate :
nonconjugated
reactivity :
human, mouse
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, neutralization, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, blocking or activating experiments
more info or order :
citations: 70
Published Application/Species/Sample/DilutionReference
  • western blot; mouse; 1:1000; loading ...; fig 1g, 1h
Ichikawa K, Watanabe Miyano S, Minoshima Y, Matsui J, Funahashi Y. Activated FGF2 signaling pathway in tumor vasculature is essential for acquired resistance to anti-VEGF therapy. Sci Rep. 2020;10:2939 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:100; loading ...; fig 6a
Zhang S, Liu W, Yang Y, Sun K, Li S, Xu H, et al. Tmem30a Deficiency in endothelial cells impairs cell proliferation and angiogenesis. J Cell Sci. 2019;: pubmed publisher
  • blocking or activating experiments; mouse; loading ...; fig 3a
Jiang Y, Lin X, Tang Z, Lee C, Tian G, Du Y, et al. Critical role of caveolin-1 in ocular neovascularization and multitargeted antiangiogenic effects of cavtratin via JNK. Proc Natl Acad Sci U S A. 2017;114:10737-10742 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:100; loading ...; fig 4b
Hara T, Nakaoka H, Hayashi T, Mimura K, Hoshino D, Inoue M, et al. Control of metastatic niche formation by targeting APBA3/Mint3 in inflammatory monocytes. Proc Natl Acad Sci U S A. 2017;114:E4416-E4424 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:80; loading ...; fig 3b
Lajko M, Cardona H, Taylor J, Shah R, Farrow K, Fawzi A. Hyperoxia-Induced Proliferative Retinopathy: Early Interruption of Retinal Vascular Development with Severe and Irreversible Neurovascular Disruption. PLoS ONE. 2016;11:e0166886 pubmed publisher
  • immunohistochemistry; mouse; fig 3
Jo D, Bae J, Chae S, Kim J, Han J, Hwang D, et al. Quantitative Proteomics Reveals β2 Integrin-mediated Cytoskeletal Rearrangement in Vascular Endothelial Growth Factor (VEGF)-induced Retinal Vascular Hyperpermeability. Mol Cell Proteomics. 2016;15:1681-91 pubmed publisher
  • blocking or activating experiments; mouse; fig 6
Zhao C, Zhang W, Zhao Y, Yang Y, Luo H, Ji G, et al. Endothelial Cords Promote Tumor Initial Growth prior to Vascular Function through a Paracrine Mechanism. Sci Rep. 2016;6:19404 pubmed publisher
  • immunohistochemistry; mouse; 1:200; fig 5
Kim Y, Nam H, Lee J, Park D, Kim C, Yu Y, et al. Methylation-dependent regulation of HIF-1α stability restricts retinal and tumour angiogenesis. Nat Commun. 2016;7:10347 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:250
Wang J, Xiao J, Wen D, Wu X, Mao Z, Zhang J, et al. Endothelial cell-anchored tissue factor pathway inhibitor regulates tumor metastasis to the lung in mice. Mol Carcinog. 2016;55:882-96 pubmed publisher
Abdelrahman A, Sandow P, Wang J, Xu Z, Rojas M, Bomalaski J, et al. Arginine deprivation/citrulline augmentation with ADI-PEG20 as novel therapy for complications in type 2 diabetes. Mol Metab. 2024;89:102020 pubmed publisher
Kumar B, Mishra M, Talreja D, Cashman S, Kumar Singh R. Cell-Penetrating Chaperone Nuc1 for Small- and Large-Molecule Delivery Into Retinal Cells and Tissues. Invest Ophthalmol Vis Sci. 2024;65:31 pubmed publisher
Tai Y, Chow A, Han S, Coker C, Ma W, Gu Y, et al. FLT1 activation in cancer cells promotes PARP-inhibitor resistance in breast cancer. EMBO Mol Med. 2024;16:1957-1980 pubmed publisher
Furue A, Hattori K, Hosono K, Tanabe M, Sato E, Honda M, et al. Inhibition of TP signaling promotes endometriosis growth and neovascularization. Mol Med Rep. 2023;28: pubmed publisher
Michail A, Gkikas D, Stellas D, Kaltezioti V, Politis P. Prox1 Suppresses the Proliferation of Breast Cancer Cells via Direct Inhibition of c-Myc Gene Expression. Cells. 2023;12: pubmed publisher
Damon Soubeyrand C, Bongiovanni A, Chorfa A, Goubely C, Pirot N, Pardanaud L, et al. Three-dimensional imaging of vascular development in the mouse epididymis. elife. 2023;12: pubmed publisher
Denninger J, Miller L, Walters A, Hosawi M, Sebring G, Rieskamp J, et al. Neural stem and progenitor cells support and protect adult hippocampal function via vascular endothelial growth factor secretion. bioRxiv. 2023;: pubmed publisher
Liu S, Hur Y, Cai X, Cong Q, Yang Y, Xu C, et al. A tissue injury sensing and repair pathway distinct from host pathogen defense. Cell. 2023;186:2127-2143.e22 pubmed publisher
Wei J, Lee W, Luo J, Huang H, Wang S, Chou R, et al. Vertical Sleeve Gastrectomy Offers Protection against Disturbed Flow-Induced Atherosclerosis in High-Fat Diet-Fed Mice. Int J Mol Sci. 2023;24: pubmed publisher
Lee E, Lee E, Kong E, Chon H, Llaiqui Condori M, Park C, et al. An agonistic anti-Tie2 antibody suppresses the normal-to-tumor vascular transition in the glioblastoma invasion zone. Exp Mol Med. 2023;55:470-484 pubmed publisher
Huang J, Mao Y, Li Q, Hong H, Tang N, Kang X, et al. Kallistatin prevents ovarian hyperstimulation syndrome by regulating vascular leakage. J Cell Mol Med. 2022;26:4613-4623 pubmed publisher
Tamura S, Mukaide M, Katsuragi Y, Fujii W, Odaira K, Suzuki N, et al. Periosteum-derived podoplanin-expressing stromal cells regulate nascent vascularization during epiphyseal marrow development. J Biol Chem. 2022;298:101833 pubmed publisher
Fu J, Shinjo T, Li Q, St Louis R, Park K, Yu M, et al. Regeneration of glomerular metabolism and function by podocyte pyruvate kinase M2 in diabetic nephropathy. JCI Insight. 2022;7: pubmed publisher
Fan L, Kan H, Chen X, Sun Y, Chen L, Shen W. Vascular endothelial growth factor-A/vascular endothelial growth factor2 signaling in spinal neurons contributes to bone cancer pain. Mol Pain. 2022;18:17448069221075891 pubmed publisher
Yang C, Feng X, Li Z, He Q. Plumbagin inhibits tumor angiogenesis of gastric carcinoma in mice by modulating nuclear factor-kappa B pathway. Transl Cancer Res. 2020;9:556-564 pubmed publisher
Martin Rodriguez O, Gauthier T, Bonnefoy F, Couturier M, Daoui A, Chagu xe9 C, et al. Pro-Resolving Factors Released by Macrophages After Efferocytosis Promote Mucosal Wound Healing in Inflammatory Bowel Disease. Front Immunol. 2021;12:754475 pubmed publisher
Yaguchi A, Oshikawa M, Watanabe G, Hiramatsu H, Uchida N, Hara C, et al. Efficient protein incorporation and release by a jigsaw-shaped self-assembling peptide hydrogel for injured brain regeneration. Nat Commun. 2021;12:6623 pubmed publisher
Kataru R, Baik J, Park H, Ly C, Shin J, Schwartz N, et al. Lymphatic-specific intracellular modulation of receptor tyrosine kinase signaling improves lymphatic growth and function. Sci Signal. 2021;14: pubmed publisher
Mirzapour Shafiyi F, Kametani Y, Hikita T, Hasegawa Y, Nakayama M. Numerical evaluation reveals the effect of branching morphology on vessel transport properties during angiogenesis. PLoS Comput Biol. 2021;17:e1008398 pubmed publisher
Perelli R, O Sullivan M, Zarnick S, Kay J. Environmental oxygen regulates astrocyte proliferation to guide angiogenesis during retinal development. Development. 2021;148: pubmed publisher
Philipp D, Holthaus M, Basoah V, Pfannkuche K, Suhr L, Wahlers T, et al. VEGF Contributes to Mesenchymal Stem Cell-Mediated Reversion of Nor1-Dependent Hypertrophy in iPS Cell-Derived Cardiomyocytes. Stem Cells Int. 2021;2021:8888575 pubmed publisher
Díaz Lezama N, Wolf A, Koch S, Pfaller A, Biber J, Guillonneau X, et al. PDGF Receptor Alpha Signaling Is Key for Müller Cell Homeostasis Functions. Int J Mol Sci. 2021;22: pubmed publisher
Yun H, Yee M, Lathrop K, Kinchington P, Hendricks R, St Leger A. Production of the Cytokine VEGF-A by CD4+ T and Myeloid Cells Disrupts the Corneal Nerve Landscape and Promotes Herpes Stromal Keratitis. Immunity. 2020;53:1050-1062.e5 pubmed publisher
Li S, Liu M, Do M, Chou C, Stamatiades E, Nixon B, et al. Cancer immunotherapy via targeted TGF-β signalling blockade in TH cells. Nature. 2020;587:121-125 pubmed publisher
Mulcrone P, Edwards S, Petrusca D, Haneline L, Delgado Calle J, Roodman G. Osteocyte Vegf-a contributes to myeloma-associated angiogenesis and is regulated by Fgf23. Sci Rep. 2020;10:17319 pubmed publisher
Zheng Q, Dai X, Fang W, Zheng Y, Zhang J, Liu Y, et al. Overexpression of microRNA-367 inhibits angiogenesis in ovarian cancer by downregulating the expression of LPA1. Cancer Cell Int. 2020;20:476 pubmed publisher
Shao Y, Chen J, Li X, Ma J. Detection and Quantification of Retinal Neovascularization Using BrdU Incorporation. Transl Vis Sci Technol. 2020;9:4 pubmed publisher
Zhu L, Parker M, Enemchukwu N, Shen M, Zhang G, Yan Q, et al. Combination of apolipoprotein-A-I/apolipoprotein-A-I binding protein and anti-VEGF treatment overcomes anti-VEGF resistance in choroidal neovascularization in mice. Commun Biol. 2020;3:386 pubmed publisher
Sheibani N, Zaitoun I, Wang S, Darjatmoko S, Suscha A, Song Y, et al. Inhibition of retinal neovascularization by a PEDF-derived nonapeptide in newborn mice subjected to oxygen-induced ischemic retinopathy. Exp Eye Res. 2020;195:108030 pubmed publisher
Wang Z, Wu C, Zhang M, Dong A, Niu R, Zhang J. Sevoflurane promotes the proliferation of HUVECs by activating VEGF signaling. Exp Ther Med. 2020;19:1336-1342 pubmed publisher
Wu J, Li Y, Chen A, Hong C, Zhang C, Wang H, et al. Inhibition of Sema4D/PlexinB1 signaling alleviates vascular dysfunction in diabetic retinopathy. EMBO Mol Med. 2020;12:e10154 pubmed publisher
Xu N, Bo Q, Shao R, Liang J, Zhai Y, Yang S, et al. Chitinase-3-Like-1 Promotes M2 Macrophage Differentiation and Induces Choroidal Neovascularization in Neovascular Age-Related Macular Degeneration. Invest Ophthalmol Vis Sci. 2019;60:4596-4605 pubmed publisher
Sheibani N, Wang S, Darjatmoko S, Fisk D, Shahi P, Pattnaik B, et al. Novel anti-angiogenic PEDF-derived small peptides mitigate choroidal neovascularization. Exp Eye Res. 2019;188:107798 pubmed publisher
Fan Y, Zhao L, Xie W, Yi D, He S, Chen D, et al. Serum miRNAs are potential biomarkers for the detection of disc degeneration, among which miR-26a-5p suppresses Smad1 to regulate disc homeostasis. J Cell Mol Med. 2019;23:6679-6689 pubmed publisher
Oe Y, Fushima T, Sato E, Sekimoto A, Kisu K, Sato H, et al. Protease-activated receptor 2 protects against VEGF inhibitor-induced glomerular endothelial and podocyte injury. Sci Rep. 2019;9:2986 pubmed publisher
Kolb A, Shupp A, Mukhopadhyay D, Marini F, Bussard K. Osteoblasts are "educated" by crosstalk with metastatic breast cancer cells in the bone tumor microenvironment. Breast Cancer Res. 2019;21:31 pubmed publisher
John B, Naczki C, Patel C, Ghoneum A, Qasem S, Salih Z, et al. Regulation of the bi-directional cross-talk between ovarian cancer cells and adipocytes by SPARC. Oncogene. 2019;38:4366-4383 pubmed publisher
Balser C, Wolf A, Herb M, Langmann T. Co-inhibition of PGF and VEGF blocks their expression in mononuclear phagocytes and limits neovascularization and leakage in the murine retina. J Neuroinflammation. 2019;16:26 pubmed publisher
Nakayama T, Nagata E, Masuda H, Asahara T, Takizawa S. Regeneration-associated cell transplantation contributes to tissue recovery in mice with acute ischemic stroke. PLoS ONE. 2019;14:e0210198 pubmed publisher
Zhou T, Zheng Y, Sun L, Badea S, Jin Y, Liu Y, et al. Microvascular endothelial cells engulf myelin debris and promote macrophage recruitment and fibrosis after neural injury. Nat Neurosci. 2019;22:421-435 pubmed publisher
Subileau M, Merdzhanova G, Ciais D, Collin Faure V, Feige J, Bailly S, et al. Bone Morphogenetic Protein 9 Regulates Early Lymphatic-Specified Endothelial Cell Expansion during Mouse Embryonic Stem Cell Differentiation. Stem Cell Reports. 2019;12:98-111 pubmed publisher
Nishida Y, Yamada Y, Kanemaru H, Ohazama A, Maeda T, Seo K. Vascularization via activation of VEGF-VEGFR signaling is essential for peripheral nerve regeneration. Biomed Res. 2018;39:287-294 pubmed publisher
Adrian Segarra J, Sreenivasan K, Gajawada P, Lörchner H, Braun T, Pöling J. The AB loop of oncostatin M (OSM) determines species-specific signaling in humans and mice. J Biol Chem. 2018;293:20181-20199 pubmed publisher
Schrand B, Clark E, Levay A, Capote A, Martínez O, Brenneman R, et al. Hapten-mediated recruitment of polyclonal antibodies to tumors engenders antitumor immunity. Nat Commun. 2018;9:3348 pubmed publisher
Ding J, Tan X, Song K, Ma W, Xiao J, Song Y, et al. Bushen Huoxue Recipe Alleviates Implantation Loss in Mice by Enhancing Estrogen-Progesterone Signals and Promoting Decidual Angiogenesis Through FGF2 During Early Pregnancy. Front Pharmacol. 2018;9:437 pubmed publisher
Kobori T, Hamasaki S, Kitaura A, Yamazaki Y, Nishinaka T, Niwa A, et al. Interleukin-18 Amplifies Macrophage Polarization and Morphological Alteration, Leading to Excessive Angiogenesis. Front Immunol. 2018;9:334 pubmed publisher
Chen J, Qiao Y, Tang B, Chen G, Liu X, Yang B, et al. Modulation of Salmonella Tumor-Colonization and Intratumoral Anti-angiogenesis by Triptolide and Its Mechanism. Theranostics. 2017;7:2250-2260 pubmed publisher
Chen X, Yu T, Zhang J, Li Y, Chen H, Yang G, et al. CYP4A in tumor-associated macrophages promotes pre-metastatic niche formation and metastasis. Oncogene. 2017;36:5045-5057 pubmed publisher
Basavarajappa H, Sulaiman R, Qi X, Shetty T, Sheik Pran Babu S, Sishtla K, et al. Ferrochelatase is a therapeutic target for ocular neovascularization. EMBO Mol Med. 2017;9:786-801 pubmed publisher
Reina Torres E, Wen J, Liu K, Li G, Sherwood J, Chang J, et al. VEGF as a Paracrine Regulator of Conventional Outflow Facility. Invest Ophthalmol Vis Sci. 2017;58:1899-1908 pubmed publisher
Morita A, Ushikubo H, Mori A, Arima S, Sakamoto K, Nagamitsu T, et al. A delay in vascularization induces abnormal astrocyte proliferation and migration in the mouse retina. Dev Dyn. 2017;246:186-200 pubmed publisher
Kragl M, Schubert R, Karsjens H, Otter S, Bartosinska B, Jeruschke K, et al. The biomechanical properties of an epithelial tissue determine the location of its vasculature. Nat Commun. 2016;7:13560 pubmed publisher
Sun J, Jiao K, Niu L, Jiao Y, Song Q, Shen L, et al. Intrafibrillar silicified collagen scaffold modulates monocyte to promote cell homing, angiogenesis and bone regeneration. Biomaterials. 2017;113:203-216 pubmed publisher
Zheng L, Zhao C, Du Y, Lin X, Jiang Y, Lee C, et al. PDGF-CC underlies resistance to VEGF-A inhibition and combinatorial targeting of both suppresses pathological angiogenesis more efficiently. Oncotarget. 2016;7:77902-77915 pubmed publisher
Wang H, Han X, Bretz C, Becker S, Gambhir D, Smith G, et al. Retinal pigment epithelial cell expression of active Rap 1a by scAAV2 inhibits choroidal neovascularization. Mol Ther Methods Clin Dev. 2016;3:16056 pubmed publisher
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product information
master code :
AF-493-NA
SKU :
AF-493-NA
product name :
Mouse VEGF164 Antibody
unit size :
100 ug (also 25 ug)
description :
The Mouse VEGF164 Antibody from R&D Systems is a goat polyclonal antibody to VEGF. This antibody reacts with human,mouse,n/a,rabbit,transgenic mouse. The Mouse VEGF164 Antibody has been validated for the following applications: Neutralization,Immunohistochemistry,ELISA Capture,In vivo assay,Western Blot,Blocking,ELISA Development,ELISA Development (Capture),ELISA Development (Detection),Luminex Development,Functional Assay,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunocytochemistry,ELISA Capture (Matched Antibody Pair).
target :
VEGF
category :
Primary Antibodies
buffer :
Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.
clonality :
Polyclonal
concentration :
LYOPH
conjugate :
Unconjugated
host :
Goat
immunogen :
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse VEGF164, Ala27-Arg190, Accession # AAA40547
isotype :
IgG
purity :
Antigen Affinity-purified
species :
Human,Mouse,Rabbit,Transgenic Mouse
specificity :
Detects mouse VEGF in ELISAs and Western blots. In Western blots, detection of recombinant mouse (rm) VEGF164, rmVEGF120, rmVEGF188, and recombinant rat VEGF164 is observered but no cross-reactivity with rmVEGF-B167,rmVEGF-B186, or recombinant human (rh) VEGF165 is observed. In sandwich immunoassays, less than 0.04% cross-reactivity with rhVEGF121, rhVEGF165, and rhVEGF/PlGF is observed.
gene symbol :
VEGFA
endotoxin note :
<0.10 EU per 1 ╡g of the antibody by the LAL method.
top caption :
Detection of Recombinant Human, Mouse, and Rat VEGF antibody by Western Blot.
accessionNumbers :
AAA40547
applications :
In vivo assay,Neutralization,Western Blot,Immunohistochemistry,Blocking,ELISA Development,ELISA Development (Capture),ELISA Development (Detection),Luminex Development,Functional Assay,ELISA Capture,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunocytochemistry,ELISA Capture (Matched Antibody Pair)
USD :
519 USD
alt names :
MVCD1, VAS, vascular endothelial growth factor A, Vascular permeability factor, Vasculotropin, VEGFA, VEGF-A, VEGFMGC70609, VPF, VPFvascular endothelial growth factor
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.
more info or order :
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.