product summary
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company name :
R&D Systems
product type :
antibody
product name :
Human/Mouse TNF-alpha Antibody
catalog :
AF-410-NA
quantity :
100 ug (also 25 ug)
price :
509 USD
clonality :
polyclonal
host :
domestic goat
conjugate :
nonconjugated
reactivity :
human, mouse, rat
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, neutralization, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, blocking or activating experiments
more info or order :
citations: 52
Published Application/Species/Sample/DilutionReference
  • blocking or activating experiments; human; 3 ug/ml; loading ...; fig 4a
Zhou Q, Wu X, Wang X, Yu Z, Pan T, Li Z, et al. The reciprocal interaction between tumor cells and activated fibroblasts mediated by TNF-α/IL-33/ST2L signaling promotes gastric cancer metastasis. Oncogene. 2019;: pubmed publisher
  • blocking or activating experiments; mouse; 0.5 ug/ml; loading ...; fig 1f
Sul O, Rajasekaran M, Park H, Suh J, Choi H. MicroRNA-29b Enhances Osteoclast Survival by Targeting BCL-2-Modifying Factor after Lipopolysaccharide Stimulation. Oxid Med Cell Longev. 2019;2019:6018180 pubmed publisher
  • ELISA; mouse; fig 6
Chuang T, Guo Y, Seki S, Rosen A, Johanson D, Mandell J, et al. LRP1 expression in microglia is protective during CNS autoimmunity. Acta Neuropathol Commun. 2016;4:68 pubmed publisher
  • blocking or activating experiments; mouse; fig 5
Xu X, Meng Q, Erben U, Wang P, Glauben R, Kuhl A, et al. Myeloid-derived suppressor cells promote B-cell production of IgA in a TNFR2-dependent manner. Cell Mol Immunol. 2017;14:597-606 pubmed publisher
Zhong X, Wu H, Zhang W, Shi Y, Gwack Y, Xue H, et al. Distinct RORγt-dependent Th17 immune responses are required for autoimmune pathogenesis and protection against bacterial infection. Cell Rep. 2024;43:114951 pubmed publisher
Liu A, Hayashi M, Ohsugi Y, Katagiri S, Akira S, Iwata T, et al. The IL-33/ST2 axis is protective against acute inflammation during the course of periodontitis. Nat Commun. 2024;15:2707 pubmed publisher
Zhou X, Hao H, Chen Y, Cao W, Zhu Z, Ni Y, et al. Covalently grafted human serum albumin coating mitigates the foreign body response against silicone implants in mice. Bioact Mater. 2024;34:482-493 pubmed publisher
O Neill A, Mantri C, Tan C, Saron W, Nagaraj S, Kala M, et al. Mucosal SARS-CoV-2 vaccination of rodents elicits superior systemic T central memory function and cross-neutralising antibodies against variants of concern. EBioMedicine. 2024;99:104924 pubmed publisher
Oppezzo A, Monney L, Kilian H, Slimani L, Maczkowiak Chartois F, Rosselli F. Fanca deficiency is associated with alterations in osteoclastogenesis that are rescued by TNFα. Cell Biosci. 2023;13:115 pubmed publisher
Capellmann S, Sonntag R, Sch xfc ler H, Meurer S, Gan L, Kauffmann M, et al. Transformation of primary murine peritoneal mast cells by constitutive KIT activation is accompanied by loss of Cdkn2a/Arf expression. Front Immunol. 2023;14:1154416 pubmed publisher
Theocharidis G, Rahmani S, Lee S, Li Z, Lobao A, Kounas K, et al. Murine macrophages or their secretome delivered in alginate dressings enhance impaired wound healing in diabetic mice. Biomaterials. 2022;288:121692 pubmed publisher
Hwang Y, Kim H, Shin E. Effect of rottlerin on astrocyte phenotype polarization after trimethyltin insult in the dentate gyrus of mice. J Neuroinflammation. 2022;19:142 pubmed publisher
Picod A, Martino S, Cervera P, Manuceau G, Arca M, Wittner M, et al. Deregulated JAK3 mediates growth advantage and hemophagocytosis in extranodal nasal-type natural killer/T-cell lymphoma. Haematologica. 2022;107:2218-2225 pubmed publisher
Bai B, Li H, Han L, Mei Y, Hu C, Mei Q, et al. Molecular mechanism of the TGF‑β/Smad7 signaling pathway in ulcerative colitis. Mol Med Rep. 2022;25: pubmed publisher
Neufeldt C, Cerikan B, Cortese M, Frankish J, Lee J, Plociennikowska A, et al. SARS-CoV-2 infection induces a pro-inflammatory cytokine response through cGAS-STING and NF-κB. Commun Biol. 2022;5:45 pubmed publisher
Theocharidis G, Thomas B, Sarkar D, Mumme H, Pilcher W, Dwivedi B, et al. Single cell transcriptomic landscape of diabetic foot ulcers. Nat Commun. 2022;13:181 pubmed publisher
Luo Y, Yao F, Hu X, Li Y, Chen Y, Li Z, et al. M1 macrophages impair tight junctions between endothelial cells after spinal cord injury. Brain Res Bull. 2022;180:59-72 pubmed publisher
Kim G, Choi K, Kim H, Chung D. Alleviation of LPS-Induced Inflammation and Septic Shock by Lactiplantibacillus plantarum K8 Lysates. Int J Mol Sci. 2021;22: pubmed publisher
Kothari V, Tang J, He Y, Kramer F, Kanter J, Bornfeldt K. ADAM17 Boosts Cholesterol Efflux and Downstream Effects of High-Density Lipoprotein on Inflammatory Pathways in Macrophages. Arterioscler Thromb Vasc Biol. 2021;41:1854-1873 pubmed publisher
AlQranei M, Senbanjo L, Aljohani H, Hamza T, Chellaiah M. Lipopolysaccharide- TLR-4 Axis regulates Osteoclastogenesis independent of RANKL/RANK signaling. BMC Immunol. 2021;22:23 pubmed publisher
Najjar R, Akhavan N, Pourafshar S, Arjmandi B, Feresin R. Cornus officinalis var. koreana Kitam polyphenol extract decreases pro-inflammatory markers in lipopolysaccharide (LPS)-induced RAW 264.7 macrophages by reducing Akt phosphorylation. J Ethnopharmacol. 2021;270:113734 pubmed publisher
Shao B, Wang L, Yu Y, Chen L, Gan N, Huang W. Effects of CD4+ T lymphocytes from ovariectomized mice on bone marrow mesenchymal stem cell proliferation and osteogenic differentiation. Exp Ther Med. 2020;20:84 pubmed publisher
Liu S, Wang Z, Su Y, Ray R, Jing X, Wang Y, et al. Somatotopic Organization and Intensity Dependence in Driving Distinct NPY-Expressing Sympathetic Pathways by Electroacupuncture. Neuron. 2020;108:436-450.e7 pubmed publisher
Yoshida T, Das N, Carpenter A, Izadpanah R, Kumar S, Gautam S, et al. Minocycline reverses IL-17A/TRAF3IP2-mediated p38 MAPK/NF-κB/iNOS/NO-dependent cardiomyocyte contractile depression and death. Cell Signal. 2020;73:109690 pubmed publisher
Li Z, Zhang C, Du J, Zhao J, Shi M, Jin M, et al. Adipocytes promote tumor progression and induce PD-L1 expression via TNF-α/IL-6 signaling. Cancer Cell Int. 2020;20:179 pubmed publisher
Takagaki Y, Lee S, Dongqing Z, Kitada M, Kanasaki K, Koya D. Endothelial autophagy deficiency induces IL6 - dependent endothelial mesenchymal transition and organ fibrosis. Autophagy. 2020;:1-10 pubmed publisher
Liu X, Tonello R, Ling Y, Gao Y, Berta T. Paclitaxel-activated astrocytes produce mechanical allodynia in mice by releasing tumor necrosis factor-α and stromal-derived cell factor 1. J Neuroinflammation. 2019;16:209 pubmed publisher
Xu Y, Xu Y, Zhu Y, Sun H, Juguilon C, Li F, et al. Macrophage miR-34a Is a Key Regulator of Cholesterol Efflux and Atherosclerosis. Mol Ther. 2020;28:202-216 pubmed publisher
Wang P, Qi X, Xu G, Liu J, Guo J, Li X, et al. CCL28 promotes locomotor recovery after spinal cord injury via recruiting regulatory T cells. Aging (Albany NY). 2019;11:7402-7415 pubmed publisher
Kim S, Neuendorff N, Earnest D. Role of Proinflammatory Cytokines in Feedback Modulation of Circadian Clock Gene Rhythms by Saturated Fatty Acids. Sci Rep. 2019;9:8909 pubmed publisher
Zhang X, He D, Gao S, Wei Y, Wang L. Aspergillus fumigatus enhances human NK cell activity by regulating M1 macrophage polarization. Mol Med Rep. 2019;20:1241-1249 pubmed publisher
Pido Lopez J, Tanudjojo B, Farag S, Bondulich M, Andre R, Tabrizi S, et al. Inhibition of tumour necrosis factor alpha in the R6/2 mouse model of Huntington's disease by etanercept treatment. Sci Rep. 2019;9:7202 pubmed publisher
Evgin L, Huff A, Kottke T, Thompson J, Molan A, Driscoll C, et al. Suboptimal T-cell Therapy Drives a Tumor Cell Mutator Phenotype That Promotes Escape from First-Line Treatment. Cancer Immunol Res. 2019;7:828-840 pubmed publisher
Rosen D, Seki S, Fernandez Castaneda A, Beiter R, Eccles J, Woodfolk J, et al. Modulation of the sigma-1 receptor-IRE1 pathway is beneficial in preclinical models of inflammation and sepsis. Sci Transl Med. 2019;11: pubmed publisher
Wixey J, Lee K, Miller S, Goasdoue K, Colditz P, Tracey Bjorkman S, et al. Neuropathology in intrauterine growth restricted newborn piglets is associated with glial activation and proinflammatory status in the brain. J Neuroinflammation. 2019;16:5 pubmed publisher
Widmann C, Gandin C, Petit Paitel A, Lazdunski M, Heurteaux C. The Traditional Chinese Medicine MLC901 inhibits inflammation processes after focal cerebral ischemia. Sci Rep. 2018;8:18062 pubmed publisher
Righetti R, Dos Santos T, Camargo L, Aristóteles L, Fukuzaki S, de Souza F, et al. Protective Effects of Anti-IL17 on Acute Lung Injury Induced by LPS in Mice. Front Pharmacol. 2018;9:1021 pubmed publisher
Kremer M, Yalcin I, Goumon Y, Wurtz X, Nexon L, Daniel D, et al. A Dual Noradrenergic Mechanism for the Relief of Neuropathic Allodynia by the Antidepressant Drugs Duloxetine and Amitriptyline. J Neurosci. 2018;38:9934-9954 pubmed publisher
Lee C, Moon S, Jeong J, Lee S, Lee M, Yoo S, et al. Kaempferol targeting on the fibroblast growth factor receptor 3-ribosomal S6 kinase 2 signaling axis prevents the development of rheumatoid arthritis. Cell Death Dis. 2018;9:401 pubmed publisher
Zenke K, Muroi M, Tanamoto K. AKT1 distinctively suppresses MyD88-depenedent and TRIF-dependent Toll-like receptor signaling in a kinase activity-independent manner. Cell Signal. 2018;43:32-39 pubmed publisher
Kottke T, Evgin L, Shim K, Rommelfanger D, Boisgerault N, Zaidi S, et al. Subversion of NK-cell and TNF? Immune Surveillance Drives Tumor Recurrence. Cancer Immunol Res. 2017;5:1029-1045 pubmed publisher
Lee S, Kwon J, Kim S, Jung K, Cho M. Interferon-gamma regulates inflammatory cell death by targeting necroptosis in experimental autoimmune arthritis. Sci Rep. 2017;7:10133 pubmed publisher
Oh Y, Kwon Y, Jung B. Anti-inflammatory Effects of the Natural Compounds Cortex Phellodendri and Humulus japonicus on Pelvic Inflammatory Disease in Mice. Int J Med Sci. 2017;14:729-734 pubmed publisher
Matsushita N, Hassanein M, Martínez Clemente M, Lazaro R, French S, Xie W, et al. Gender difference in NASH susceptibility: Roles of hepatocyte Ikk? and Sult1e1. PLoS ONE. 2017;12:e0181052 pubmed publisher
Chung K, Chatzigeorgiou A, Economopoulou M, Garcia Martin R, Alexaki V, Mitroulis I, et al. A self-sustained loop of inflammation-driven inhibition of beige adipogenesis in obesity. Nat Immunol. 2017;18:654-664 pubmed publisher
Tsunekawa T, Banno R, Mizoguchi A, Sugiyama M, Tominaga T, Onoue T, et al. Deficiency of PTP1B Attenuates Hypothalamic Inflammation via Activation of the JAK2-STAT3 Pathway in Microglia. EBioMedicine. 2017;16:172-183 pubmed publisher
Dempsey C, Rubio Araiz A, Bryson K, Finucane O, Larkin C, Mills E, et al. Inhibiting the NLRP3 inflammasome with MCC950 promotes non-phlogistic clearance of amyloid-? and cognitive function in APP/PS1 mice. Brain Behav Immun. 2017;61:306-316 pubmed publisher
Munoz M, Burghi V, Miquet J, Cervino I, Quiroga D, Mazziotta L, et al. Chronic blockade of the AT2 receptor with PD123319 impairs insulin signaling in C57BL/6 mice. Peptides. 2017;88:37-45 pubmed publisher
Ha S, Sung J, Choi I, Kim Y. Oryza sativa (Rice) Hull Extract Inhibits Lipopolysaccharide-Induced Inflammatory Response in RAW264.7 Macrophages by Suppressing Extracellular Signal-regulated Kinase, c-Jun N-terminal Kinase, and Nuclear Factor-κB Activation. Pharmacogn Mag. 2016;12:295-301 pubmed
Lim S, Yuzhalin A, Gordon Weeks A, Muschel R. Tumor-infiltrating monocytes/macrophages promote tumor invasion and migration by upregulating S100A8 and S100A9 expression in cancer cells. Oncogene. 2016;35:5735-5745 pubmed publisher
Kremer M, Yalcin I, Nexon L, Wurtz X, Ceredig R, Daniel D, et al. The antiallodynic action of pregabalin in neuropathic pain is independent from the opioid system. Mol Pain. 2016;12: pubmed publisher
Teramachi J, Silbermann R, Yang P, Zhao W, Mohammad K, Guo J, et al. Blocking the ZZ domain of sequestosome1/p62 suppresses myeloma growth and osteoclast formation in vitro and induces dramatic bone formation in myeloma-bearing bones in vivo. Leukemia. 2016;30:390-8 pubmed publisher
product information
master code :
AF-410-NA
SKU :
AF-410-NA
product name :
Human/Mouse TNF-alpha Antibody
unit size :
100 ug (also 25 ug)
description :
The Human/Mouse TNF-alpha Antibody from R&D Systems is a goat polyclonal antibody to TNF-alpha. This antibody reacts with human,mouse,n/a,porcine,rat. The Human/Mouse TNF-alpha Antibody has been validated for the following applications: Neutralization,Functional Assay,Immunocytochemistry,ELISA Capture,Western Blot,Immunohistochemistry,Intracellular Staining by Flow Cytometry,Assay Development,Bioassay,ELISA Development,ELISA Development (Capture),Luminex Development,Flow Cytometry,Immunoprecipitation,Cell Culture,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,In vivo assay,ELISA Capture (Matched Antibody Pair),CyTOF-ready.
target :
TNF-alpha
category :
Primary Antibodies
buffer :
Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.
clonality :
Polyclonal
concentration :
LYOPH
conjugate :
Unconjugated
host :
Goat
immunogen :
E. coli -derived recombinant mouse TNF-alpha , Leu80-Leu235, Accession # P06804
isotype :
IgG
purity :
Antigen Affinity-purified
species :
Human,Mouse,N/A,Porcine,Rat
specificity :
Detects mouse TNF-alpha in ELISAs. Detects human and mouse TNF-alpha in Western blots. In sandwich immunoassays, approximately 50% cross-reactivity with recombinant rat TNF-alpha is observed.
gene symbol :
TNF
endotoxin note :
<0.10 EU per 1 ╡g of the antibody by the LAL method.
top caption :
Detection of Human TNF-a antibody by Western Blot.
accessionNumbers :
P06804
applications :
ELISA Capture,Western Blot,Neutralization,Immunohistochemistry,Intracellular Staining by Flow Cytometry,Assay Development,Bioassay,ELISA Development,ELISA Development (Capture),Luminex Development,Flow Cytometry,Immunoprecipitation,Functional Assay,Cell Culture,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,In vivo assay,Immunocytochemistry,ELISA Capture (Matched Antibody Pair),CyTOF-ready
USD :
509 USD
alt names :
APC1 protein, Cachectin, Cachetin, DIF, TNF, TNF, monocyte-derived, TNF-a, TNFA, TNFalpha, TNF-alphacachectin, TNFATNF, macrophage-derived, TNFG1F, TNFSF1A, TNFSF2, TNFSF2TNF superfamily, member 2, tumor necrosis factor, tumor necrosis factor (TNF superfamily, member 2), tumor necrosis factor alpha, Tumor necrosis factor ligand superfamily member 2, tumor necrosis factor-alpha
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.
more info or order :
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.