product summary
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company name :
R&D Systems
product type :
antibody
product name :
Human CD28 Antibody
catalog :
AF-342-PB
quantity :
200 ug (also 25 ug)
price :
529 USD
clonality :
polyclonal
host :
domestic goat
conjugate :
nonconjugated
reactivity :
human, mouse
application :
immunohistochemistry, immunocytochemistry, flow cytometry
more info or order :
citations: 5
Reference
Popugailo A, Rotfogel Z, Levy M, Turgeman O, Hillman D, Levy R, et al. The homodimer interfaces of costimulatory receptors B7 and CD28 control their engagement and pro-inflammatory signaling. J Biomed Sci. 2023;30:49 pubmed publisher
Yu H, Cui S, Mei Y, Li Q, Wu L, Duan S, et al. Mesangial Cells Exhibit Features of Antigen-Presenting Cells and Activate CD4+ T Cell Responses. J Immunol Res. 2019;2019:2121849 pubmed publisher
Banerjee P, Ries M, Janaka S, Grandea A, Wiseman R, O Connor D, et al. Diversification of Bw4 Specificity and Recognition of a Nonclassical MHC Class I Molecule Implicated in Maternal-Fetal Tolerance by Killer Cell Ig-like Receptors of the Rhesus Macaque. J Immunol. 2018;201:2776-2786 pubmed publisher
Boyette L, Macedo C, Hadi K, Elinoff B, Walters J, Ramaswami B, et al. Phenotype, function, and differentiation potential of human monocyte subsets. PLoS ONE. 2017;12:e0176460 pubmed publisher
Ellinghaus U, Cortini A, Pinder C, Le Friec G, Kemper C, Vyse T. Dysregulated CD46 shedding interferes with Th1-contraction in systemic lupus erythematosus. Eur J Immunol. 2017;47:1200-1210 pubmed publisher
product information
brand :
R&D Systems
master code :
AF-342-PB
SKU :
AF-342-PB
product name :
Human CD28 Antibody
unit size :
200 ug (also 25 ug)
seo description :
The Human CD28 Antibody from R&D Systems is a goat polyclonal antibody to CD28. This antibody reacts with human,mouse. The Human CD28 Antibody has been validated for the following applications: ELISA Development,Flow Cytometry,Functional Assay,Cell Culture,Immunohistochemistry,Agonist Activity,Immunocytochemistry,CyTOF-ready.
target :
CD28
category :
Primary Antibodies
buffer :
Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.
clonality :
Polyclonal
concentration :
LYOPH
conjugate :
Unconjugated
dilution :
Flow Cytometry 2.5 ug/10^6 cells, Immunohistochemistry 3-15 ug/mL, Agonist Activity 0.3-0.6 ug/mL, Immunocytochemistry 5-15 ug/mL, CyTOF-ready
host :
Goat
immunogen :
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD28, Asn19-Pro152, Accession # P10747
isotype :
IgG
purity :
Antigen Affinity-purified
species :
Human,Mouse
specificity :
Detects human CD28 in direct ELISAs and Western blots.
gene symbol :
CD28
endotoxin note :
<0.10 EU per 1 ╡g of the antibody by the LAL method.
accessionNumbers :
P10747
applications :
Agonist Activity,Immunocytochemistry,CyTOF-ready,ELISA Development,Flow Cytometry,Immunohistochemistry,Functional Assay,Cell Culture
USD :
529 USD
alt names :
CD28, CD28 antigen, CD28 antigen (Tp44), CD28 molecule, MGC138290, T-cell-specific surface glycoprotein CD28, Tp44
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.
more info or order :
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.