Human VEGF165 Antibody | R&D Systems AF-293-NA product information

product summary

company name :

R&D Systems

product type :

antibody

product name :

Human VEGF165 Antibody

catalog :

AF-293-NA

quantity :

100 ug (also 25 ug)

price :

529 USD

clonality :

polyclonal

host :

domestic goat

conjugate :

nonconjugated

reactivity :

human, mouse, rat, chicken, bovine

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, neutralization, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, proximity ligation assay, blocking or activating experiments

more info or order :

R&D Systems product webpage

citations: 27

Published Application/Species/Sample/Dilution	Reference
proximity ligation assay; human; 1:100; loading ...; fig 6c	Njau F, Shushakova N, Schenk H, Wulfmeyer V, Bollin R, Menne J, et al. Calcium dobesilate reduces VEGF signaling by interfering with heparan sulfate binding site and protects from vascular complications in diabetic mice. PLoS ONE. 2020;15:e0218494 pubmed publisher
blocking or activating experiments; human; fig 6b	Xu W, Li B, Guan X, Chung S, Wang Y, Yip Y, et al. Cancer cell-secreted IGF2 instigates fibroblasts and bone marrow-derived vascular progenitor cells to promote cancer progression. Nat Commun. 2017;8:14399 pubmed publisher
	Mou X, Shah J, Roye Y, Du C, Musah S. An ultrathin membrane mediates tissue-specific morphogenesis and barrier function in a human kidney chip. Sci Adv. 2024;10:eadn2689 pubmed publisher
	Li R, Weng X, Hu X, Wang J, Zheng L. Pigment epithelium‑derived factor inhibits proliferation, invasion and angiogenesis, and induces ferroptosis of extravillous trophoblasts by targeting Wnt‑β‑catenin/VEGF signaling in placenta accreta spectrum. Mol Med Rep. 2024;29: pubmed publisher
	Quan B, Li Z, Yang H, Li S, Yan X, Wang Y. The splicing factor YBX1 promotes the progression of osteosarcoma by upregulating VEGF165 and downregulating VEGF165b. Heliyon. 2023;9:e18706 pubmed publisher
	Pedersen T, Fojan P, Pedersen A, Magnusson N, Gurevich L. Amperometric Biosensor for Quantitative Measurement Using Sandwich Immunoassays. Biosensors (Basel). 2023;13: pubmed publisher
	Benchama O, Malamas M, Praveen K, Ethier E, Williams M, Makriyannis A, et al. Inhibition of triple negative breast cancer-associated inflammation and progression by N- acylethanolamine acid amide hydrolase (NAAA). Sci Rep. 2022;12:22255 pubmed publisher
	Brewer W, Xet Mull A, Yu A, Sweeney M, Walton E, Tobin D. Macrophage NFATC2 mediates angiogenic signaling during mycobacterial infection. Cell Rep. 2022;41:111817 pubmed publisher
	Lehmann G, Ginsberg M, Nolan D, Rodr xed guez C, Mart xed nez Gonz xe1 lez J, Zeng S, et al. Retinal Pigment Epithelium-Secreted VEGF-A Induces Alpha-2-Macroglobulin Expression in Endothelial Cells. Cells. 2022;11: pubmed publisher
	Han H, Kim Y, Mo H, Choi S, Lee K, Rim Y, et al. Preferential stimulation of melanocytes by M2 macrophages to produce melanin through vascular endothelial growth factor. Sci Rep. 2022;12:6416 pubmed publisher
	Li P, Xin H, Lu L. Extracellular vesicle-encapsulated microRNA-424 exerts inhibitory function in ovarian cancer by targeting MYB. J Transl Med. 2021;19:4 pubmed publisher
	Murrieta Coxca J, Gutiérrez Samudio R, El Shorafa H, Groten T, Rodríguez Martínez S, Cancino Diaz M, et al. Role of IL-36 Cytokines in the Regulation of Angiogenesis Potential of Trophoblast Cells. Int J Mol Sci. 2020;22: pubmed publisher
	Dong D, Fu Y, Chen F, Zhang J, Jia H, Li J, et al. Hyperoxia sensitizes hypoxic HeLa cells to ionizing radiation by downregulating HIF‑1α and VEGF expression. Mol Med Rep. 2021;23: pubmed publisher
	Nossin Y, Farrell E, Koevoet W, Somoza R, Caplan A, Brachvogel B, et al. Angiogenic Potential of Tissue Engineered Cartilage From Human Mesenchymal Stem Cells Is Modulated by Indian Hedgehog and Serpin E1. Front Bioeng Biotechnol. 2020;8:327 pubmed publisher
	Carbone M, Lacal P, Messinese S, De Giglio L, Pozzilli C, Persechino S, et al. Multiple Sclerosis Treatment and Melanoma Development. Int J Mol Sci. 2020;21: pubmed publisher
	Donzelli S, Sacconi A, Turco C, Gallo E, Milano E, Iosue I, et al. Paracrine Signaling from Breast Cancer Cells Causes Activation of ID4 Expression in Tumor-Associated Macrophages. Cells. 2020;9: pubmed publisher
	Wu J, Li Y, Chen A, Hong C, Zhang C, Wang H, et al. Inhibition of Sema4D/PlexinB1 signaling alleviates vascular dysfunction in diabetic retinopathy. EMBO Mol Med. 2020;12:e10154 pubmed publisher
	Pehrsson S, Hölttä M, Linhardt G, Danielson R, Carlsson L. Rapid Production of Human VEGF-A following Intradermal Injection of Modified VEGF-A mRNA Demonstrated by Cutaneous Microdialysis in the Rabbit and Pig In Vivo. Biomed Res Int. 2019;2019:3915851 pubmed publisher
	Kim K, Kuang S, Song Q, Gavin T, Roseguini B. Impact of heat therapy on recovery after eccentric exercise in humans. J Appl Physiol (1985). 2019;126:965-976 pubmed publisher
	Donzelli S, Milano E, Pruszko M, Sacconi A, Masciarelli S, Iosue I, et al. Expression of ID4 protein in breast cancer cells induces reprogramming of tumour-associated macrophages. Breast Cancer Res. 2018;20:59 pubmed publisher
	Jalili R, Horecka J, Swartz J, Davis R, Persson H. Streamlined circular proximity ligation assay provides high stringency and compatibility with low-affinity antibodies. Proc Natl Acad Sci U S A. 2018;115:E925-E933 pubmed publisher
	Ikari J, Nelson A, Obaid J, Girón Martínez A, Ikari K, Makino F, et al. Reduced microRNA-503 expression augments lung fibroblast VEGF production in chronic obstructive pulmonary disease. PLoS ONE. 2017;12:e0184039 pubmed publisher
	Hettema W, Wynne C, Lang B, Altendorfer M, Czeloth N, Lohmann R, et al. A randomized, single-blind, Phase I trial (INVICTAN-1) assessing the bioequivalence and safety of BI 695502, a bevacizumab biosimilar candidate, in healthy subjects. Expert Opin Investig Drugs. 2017;26:889-896 pubmed publisher
	Yang S, Qi F, Tang C, Wang H, Qin H, Li X, et al. CD147 promotes the proliferation, invasiveness, migration and angiogenesis of human lung carcinoma cells. Oncol Lett. 2017;13:898-904 pubmed publisher
	Zheng L, Zhao C, Du Y, Lin X, Jiang Y, Lee C, et al. PDGF-CC underlies resistance to VEGF-A inhibition and combinatorial targeting of both suppresses pathological angiogenesis more efficiently. Oncotarget. 2016;7:77902-77915 pubmed publisher
	Micera A, Quaranta L, Esposito G, Floriani I, Pocobelli A, Saccà S, et al. Differential Protein Expression Profiles in Glaucomatous Trabecular Meshwork: An Evaluation Study on a Small Primary Open Angle Glaucoma Population. Adv Ther. 2016;33:252-67 pubmed publisher
	Gao Y, Zhou Z, Lu S, Huang X, Zhang C, Jiang R, et al. Chemokine CCL15 Mediates Migration of Human Bone Marrow-Derived Mesenchymal Stem Cells Toward Hepatocellular Carcinoma. Stem Cells. 2016;34:1112-22 pubmed publisher

product information

master code :

AF-293-NA

SKU :

AF-293-NA

product name :

Human VEGF165 Antibody

unit size :

100 ug (also 25 ug)

description :

The Human VEGF165 Antibody from R&D Systems is a goat polyclonal antibody to VEGF. This antibody reacts with avian - chicken,avian - quail,bovine,guinea pig,human,mouse,porcine,primate - macaca mulatta (rhesus macaque),rabbit,rat. The Human VEGF165 Antibody has been validated for the following applications: Neutralization,Immunohistochemistry,ELISA Capture,Immunocytochemistry,Western Blot,Affinity Assay,Bioassay,Column,ELISA Development,ELISA Development (Capture),ELISA Development (Detection),Immunoassay Development,ELISA,Immunoprecipitation,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Dual RNAscope ISH-IHC Compatible.

target :

VEGF

category :

Primary Antibodies

buffer :

Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.

clonality :

Polyclonal

concentration :

LYOPH

conjugate :

Unconjugated

host :

Goat

immunogen :

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human VEGF165, Ala27-Arg191, Accession # NP_001165097.1

isotype :

IgG

purity :

Antigen Affinity-purified

species :

Avian - Chicken,Avian - Quail,Bovine,Guinea Pig,Human,Mouse,Porcine,Primate - Macaca mulatta (Rhesus Macaque),Rabbit,Rat

specificity :

Detects human VEGF in direct ELISAs and Western blots.

gene symbol :

VEGFA

endotoxin note :

<0.10 EU per 1 ╡g of the antibody by the LAL method.

top caption :

Detection of Human VEGF antibody by Western Blot.

accessionNumbers :

NP_001165097.1

applications :

ELISA Capture,Immunohistochemistry,Immunocytochemistry,Neutralization,Western Blot,Affinity Assay,Bioassay,Column,ELISA Development,ELISA Development (Capture),ELISA Development (Detection),Immunoassay Development,ELISA,Immunoprecipitation,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Dual RNAscope ISH-IHC Compatible

USD :

529 USD

alt names :

MVCD1, VAS, vascular endothelial growth factor A, Vascular permeability factor, Vasculotropin, VEGFA, VEGF-A, VEGFMGC70609, VPF, VPFvascular endothelial growth factor

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage