product summary
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company name :
R&D Systems
product type :
antibody
product name :
Human IL-6 Antibody
catalog :
AF-206-NA
quantity :
100 ug (also 25 ug)
price :
519 USD
clonality :
polyclonal
host :
domestic goat
conjugate :
nonconjugated
reactivity :
human, mouse, rhesus macaque
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, neutralization, flow cytometry, chromatin immunoprecipitation, immunohistochemistry - paraffin section, proximity ligation assay
more info or order :
R&D Systems product webpage
citations: 22
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; human; loading ...; fig s5f
  • western blot; human; loading ...; fig 5h
Fernández Duran I, Quintanilla A, Tarrats N, Birch J, Hari P, Millar F, et al. Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence. Cell Death Differ. 2022;29:1267-1282 pubmed publisher
Sinclair J, Vedelago C, Ryan F, Carney M, Redd M, Lynn M, et al. Post-acute sequelae of SARS-CoV-2 cardiovascular symptoms are associated with trace-level cytokines that affect cardiomyocyte function. Nat Microbiol. 2024;9:3135-3147 pubmed publisher
Yan M, Chen X, Ye Q, Li H, Zhang L, Wang Y. IL-33-dependent NF-κB activation inhibits apoptosis and drives chemoresistance in acute myeloid leukemia. Cytokine. 2024;180:156672 pubmed publisher
Urban L, Nov xe1 k x, x10c oma M, Dvo x159 xe1 nkov xe1 B, Lacina L, x160 xe1 chov xe1 J, et al. Unravelling heterogeneous effects of cancer‑associated fibroblasts on poor prognosis markers in breast cancer EM‑G3 cell line: In vitro‑targeted treatment (anti‑IL-6, anti‑VEGF-A, anti‑MFGE8) based on tr. Oncol Rep. 2024;51: pubmed publisher
Ozmen A, Nwabuobi C, Tang Z, Guo X, Larsen K, Guller S, et al. Leptin-Mediated Induction of IL-6 Expression in Hofbauer Cells Contributes to Preeclampsia Pathogenesis. Int J Mol Sci. 2023;25: pubmed publisher
Yang J, Lin W, Tai S, Ciou Y, Chung C, Chen J, et al. Suppression of TGFβ-Induced Interleukin-6 Secretion by Sinulariolide from Soft Corals through Attenuation of the p38-NF-kB Pathway in Carcinoma Cells. Int J Mol Sci. 2023;24: pubmed publisher
Newman S, Wilson B, Mamerow D, Wollant B, Nyein H, Rosenberg Hasson Y, et al. Extending the dynamic range of biomarker quantification through molecular equalization. Nat Commun. 2023;14:4192 pubmed publisher
Zeng W, Xiong L, Wu W, Li S, Liu J, Yang L, et al. CCL18 signaling from tumor-associated macrophages activates fibroblasts to adopt a chemoresistance-inducing phenotype. Oncogene. 2022;: pubmed publisher
Sun Y, Lu Y, Chen J, Bao J, Zhang H, Sun J, et al. Effects of interleukin 1β on long noncoding RNA and mRNA expression profiles of human synovial fluid derived mesenchymal stem cells. Sci Rep. 2022;12:8432 pubmed publisher
Pereira J, Bessa C, Matos P, Jordan P. Pro-Inflammatory Cytokines Trigger the Overexpression of Tumour-Related Splice Variant RAC1B in Polarized Colorectal Cells. Cancers (Basel). 2022;14: pubmed publisher
Wik L, Nordberg N, Broberg J, Bj xf6 rkesten J, Assarsson E, Henriksson S, et al. Proximity Extension Assay in Combination with Next-Generation Sequencing for High-throughput Proteome-wide Analysis. Mol Cell Proteomics. 2021;20:100168 pubmed publisher
Burston H, Kent O, Communal L, Udaskin M, Sun R, Brown K, et al. Inhibition of relaxin autocrine signaling confers therapeutic vulnerability in ovarian cancer. J Clin Invest. 2021;131: pubmed publisher
Ji Q, Zhou L, Sui H, Yang L, Wu X, Song Q, et al. Primary tumors release ITGBL1-rich extracellular vesicles to promote distal metastatic tumor growth through fibroblast-niche formation. Nat Commun. 2020;11:1211 pubmed publisher
Hwang B, Engel L, Goueli S, Zegzouti H. A homogeneous bioluminescent immunoassay to probe cellular signaling pathway regulation. Commun Biol. 2020;3:8 pubmed publisher
Muñoz D, Yannone S, Daemen A, Sun Y, Vakar Lopez F, Kawahara M, et al. Targetable mechanisms driving immunoevasion of persistent senescent cells link chemotherapy-resistant cancer to aging. JCI Insight. 2019;5: pubmed publisher
Kolb A, Shupp A, Mukhopadhyay D, Marini F, Bussard K. Osteoblasts are "educated" by crosstalk with metastatic breast cancer cells in the bone tumor microenvironment. Breast Cancer Res. 2019;21:31 pubmed publisher
Yin Y, Hao H, Cheng Y, Zang L, Liu J, Gao J, et al. Human umbilical cord-derived mesenchymal stem cells direct macrophage polarization to alleviate pancreatic islets dysfunction in type 2 diabetic mice. Cell Death Dis. 2018;9:760 pubmed publisher
Klaesson A, Grannas K, Ebai T, Heldin J, Koos B, Leino M, et al. Improved efficiency of in situ protein analysis by proximity ligation using UnFold probes. Sci Rep. 2018;8:5400 pubmed publisher
Jalili R, Horecka J, Swartz J, Davis R, Persson H. Streamlined circular proximity ligation assay provides high stringency and compatibility with low-affinity antibodies. Proc Natl Acad Sci U S A. 2018;115:E925-E933 pubmed publisher
Sameni M, Cavallo Medved D, Franco O, Chalasani A, Ji K, Aggarwal N, et al. Pathomimetic avatars reveal divergent roles of microenvironment in invasive transition of ductal carcinoma in situ. Breast Cancer Res. 2017;19:56 pubmed publisher
Larsen J, Petersen C. SorLA in Interleukin-6 Signaling and Turnover. Mol Cell Biol. 2017;37: pubmed publisher
Kim B, Kim H, Kim S, Haegeman G, Tsang B, Dhanasekaran D, et al. Adipose Stromal Cells from Visceral and Subcutaneous Fat Facilitate Migration of Ovarian Cancer Cells via IL-6/JAK2/STAT3 Pathway. Cancer Res Treat. 2017;49:338-349 pubmed publisher
product information
brand :
R&D Systems
master code :
AF-206-NA
SKU :
AF-206-NA
product name :
Human IL-6 Antibody
unit size :
100 ug (also 25 ug)
seo description :
The Human IL-6 Antibody from R&D Systems is a goat polyclonal antibody to IL-6. This antibody reacts with human,mouse,n/a,primate - macaca mulatta (rhesus macaque). The Human IL-6 Antibody has been validated for the following applications: Bioassay,Immunohistochemistry,Western Blot,Chromatin Immunoprecipitation (ChIP),Affinity Assay,ELISA Development,Proximity Ligation Assay (PLA),Flow Cytometry,Neutralization,Functional Assay,ELISA Capture,ELISA Detection,Immunohistochemistry-Paraffin,Immunocytochemistry.
target :
IL-6
category :
Primary Antibodies
buffer :
Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.
clonality :
Polyclonal
concentration :
LYOPH
conjugate :
Unconjugated
dilution :
Neutralization 15-45 ng/mL, Immunocytochemistry 5-15 ug/mL
host :
Goat
immunogen :
E. coli -derived recombinant human IL-6, Pro29-Met212, Accession # P05231
isotype :
IgG
purity :
Antigen Affinity-purified
species :
Human,Mouse,N/A,Primate - Macaca mulatta (Rhesus Macaque)
specificity :
Detects human IL-6 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse IL-6 is observed.
gene symbol :
IL6
endotoxin note :
<0.10 EU per 1 ╡g of the antibody by the LAL method.
top caption :
IL-6 antibody in Human PBMCs by Immunocytochemistry (ICC).
accessionNumbers :
P05231
applications :
Affinity Assay,Bioassay,ELISA Development,Proximity Ligation Assay (PLA),Flow Cytometry,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Neutralization,Chromatin Immunoprecipitation (ChIP),Immunocytochemistry,Functional Assay,ELISA Capture,ELISA Detection
USD :
519 USD
alt names :
B-cell differentiation factor, B-cell stimulatory factor 2, BSF2, BSF-2, CDF, CTL differentiation factor, HSF, hybridoma growth factor, IFNB2, IFN-beta-2, IL6, IL-6, Interferon beta-2, interleukin 6 (interferon, beta 2), interleukin BSF-2, interleukin-6, MGI-2A
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.
more info or order :
R&D Systems product webpage
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.