This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
R&D Systems
product type :
protein
product name :
Recombinant Human P-Selectin/CD62P Protein, CF
catalog :
ADP3
quantity :
50 ug (also 200 ug)
price :
259 USD
citations: 13
Reference
Rolfes V, Ribeiro L, Hawwari I, Böttcher L, Rosero N, Maasewerd S, et al. Platelets Fuel the Inflammasome Activation of Innate Immune Cells. Cell Rep. 2020;31:107615 pubmed publisher
Ye Z, Zhong L, Zhu S, Wang Y, Zheng J, Wang S, et al. The P-selectin and PSGL-1 axis accelerates atherosclerosis via activation of dendritic cells by the TLR4 signaling pathway. Cell Death Dis. 2019;10:507 pubmed publisher
Perdomo J, Leung H, Ahmadi Z, Yan F, Chong J, Passam F, et al. Neutrophil activation and NETosis are the major drivers of thrombosis in heparin-induced thrombocytopenia. Nat Commun. 2019;10:1322 pubmed publisher
Domínguez Luis M, Armas González E, Herrera García A, Arce Franco M, Feria M, Vicente Manzanares M, et al. L-selectin expression is regulated by CXCL8-induced reactive oxygen species produced during human neutrophil rolling. Eur J Immunol. 2019;49:386-397 pubmed publisher
Pepin M, Mezouar S, Pegon J, Muczynski V, Adam F, Bianchini E, et al. Soluble Siglec-5 associates to PSGL-1 and displays anti-inflammatory activity. Sci Rep. 2016;6:37953 pubmed publisher
Zuchtriegel G, Uhl B, Puhr Westerheide D, Pörnbacher M, Lauber K, Krombach F, et al. Platelets Guide Leukocytes to Their Sites of Extravasation. PLoS Biol. 2016;14:e1002459 pubmed publisher
Jung C, Yeh C, Hsu R, Lee C, Shun C, Chia J. Endocarditis pathogen promotes vegetation formation by inducing intravascular neutrophil extracellular traps through activated platelets. Circulation. 2015;131:571-81 pubmed publisher
Jang M, Kim Y, Bae E, Oh T, Choi H, Lee J, et al. Saikosaponin D isolated from Bupleurum falcatum inhibits selectin-mediated cell adhesion. Molecules. 2014;19:20340-9 pubmed publisher
Schneider Hohendorf T, Rossaint J, Mohan H, Böning D, Breuer J, Kuhlmann T, et al. VLA-4 blockade promotes differential routes into human CNS involving PSGL-1 rolling of T cells and MCAM-adhesion of TH17 cells. J Exp Med. 2014;211:1833-46 pubmed publisher
Frommhold D, Kamphues A, Hepper I, Pruenster M, Lukic I, Socher I, et al. RAGE and ICAM-1 cooperate in mediating leukocyte recruitment during acute inflammation in vivo. Blood. 2010;116:841-9 pubmed publisher
Deban L, Russo R, Sironi M, Moalli F, Scanziani M, Zambelli V, et al. Regulation of leukocyte recruitment by the long pentraxin PTX3. Nat Immunol. 2010;11:328-34 pubmed publisher
Wojciechowski J, Narasipura S, Charles N, Mickelsen D, Rana K, Blair M, et al. Capture and enrichment of CD34-positive haematopoietic stem and progenitor cells from blood circulation using P-selectin in an implantable device. Br J Haematol. 2008;140:673-81 pubmed publisher
Bestebroer J, Poppelier M, Ulfman L, Lenting P, Denis C, Van Kessel K, et al. Staphylococcal superantigen-like 5 binds PSGL-1 and inhibits P-selectin-mediated neutrophil rolling. Blood. 2007;109:2936-43 pubmed
product information
brand :
R&D Systems
master code :
ADP3
SKU :
ADP3-050
product name :
Recombinant Human P-Selectin/CD62P Protein, CF
description :
The Recombinant Human P-Selectin/CD62P Protein, CF from R&D Systems is derived from CHO. The Recombinant Human P-Selectin/CD62P Protein, CF has been validated for the following applications: Bioactivity.
target :
P-Selectin/CD62P
category :
Proteins and Enzymes
unit size :
50 ug (also 200 ug)
buffer :
Lyophilized from a 0.2 µm filtered solution in PBS containing Calcium and Magnesium with Sorbitol.
conjugate :
Unconjugated
purity :
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
species :
Human
observed molecular weight :
121-124 kDa, reducing conditions
theoretical molecular weight :
80 kDa
gene symbol :
SELP
details of functionality :
Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When 5 x 104 cells/well are added to human sP-Selectin coated plates (10 µg/mL with 100 µL/well), 50% will adhere after 1 hour incubation at room temperature.
endotoxin note :
<0.10 EU per 1 µg of the protein by the LAL method.
catalog number base :
ADP3
accessionNumbers :
P16109
applications :
Bioactivity
source :
Chinese Hamster Ovary cell line, CHO-derived human P-Selectin/CD62P protein Trp42-Ala771
2020 USD :
259
2021 USD :
259 USD
alt names :
CD62P, CD62P antigen, FLJ45155, GMP140, GRMP, PADGEM, PADGEMantigen CD62), PSEL, selectin P (granule membrane protein 140kDa, antigen CD62), SELP
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.