Recombinant Human P-Selectin/CD62P Protein, CF | R&D Systems ADP3-050 product information

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human P-Selectin/CD62P Protein, CF

catalog :

ADP3-050

quantity :

50 ug (also 200 ug)

price :

325 USD

more info or order :

R&D Systems product webpage

citations: 17

Reference
Datta R, Miskolci V, Gallego L xf3 pez G, Britt E, Gillette A, Kralovec A, et al. Single cell autofluorescence imaging reveals immediate metabolic shifts of neutrophils with activation across biological systems. bioRxiv. 2024;: pubmed publisher
Yeini E, Ofek P, Pozzi S, Albeck N, Ben Shushan D, Tiram G, et al. P-selectin axis plays a key role in microglia immunophenotype and glioblastoma progression. Nat Commun. 2021;12:1912 pubmed publisher
Fitterer R, Lajqi T, Kranig S, Braun M, Theissig N, Kuss N, et al. L-Arginine Modulates Neonatal Leukocyte Recruitment in a Gestational Age-Dependent Manner. J Clin Med. 2020;9: pubmed publisher
Rolfes V, Ribeiro L, Hawwari I, Böttcher L, Rosero N, Maasewerd S, et al. Platelets Fuel the Inflammasome Activation of Innate Immune Cells. Cell Rep. 2020;31:107615 pubmed publisher
Schwarz S, Gockel L, Naggi A, Barash U, Gobec M, Bendas G, et al. Glycosaminoglycans as Tools to Decipher the Platelet Tumor Cell Interaction: A Focus on P-Selectin. Molecules. 2020;25: pubmed publisher
Ye Z, Zhong L, Zhu S, Wang Y, Zheng J, Wang S, et al. The P-selectin and PSGL-1 axis accelerates atherosclerosis via activation of dendritic cells by the TLR4 signaling pathway. Cell Death Dis. 2019;10:507 pubmed publisher
Perdomo J, Leung H, Ahmadi Z, Yan F, Chong J, Passam F, et al. Neutrophil activation and NETosis are the major drivers of thrombosis in heparin-induced thrombocytopenia. Nat Commun. 2019;10:1322 pubmed publisher
Domínguez Luis M, Armas González E, Herrera García A, Arce Franco M, Feria M, Vicente Manzanares M, et al. L-selectin expression is regulated by CXCL8-induced reactive oxygen species produced during human neutrophil rolling. Eur J Immunol. 2019;49:386-397 pubmed publisher
Pepin M, Mezouar S, Pegon J, Muczynski V, Adam F, Bianchini E, et al. Soluble Siglec-5 associates to PSGL-1 and displays anti-inflammatory activity. Sci Rep. 2016;6:37953 pubmed publisher
Zuchtriegel G, Uhl B, Puhr Westerheide D, PÃ¶rnbacher M, Lauber K, Krombach F, et al. Platelets Guide Leukocytes to Their Sites of Extravasation. PLoS Biol. 2016;14:e1002459 pubmed publisher
Jung C, Yeh C, Hsu R, Lee C, Shun C, Chia J. Endocarditis pathogen promotes vegetation formation by inducing intravascular neutrophil extracellular traps through activated platelets. Circulation. 2015;131:571-81 pubmed publisher
Jang M, Kim Y, Bae E, Oh T, Choi H, Lee J, et al. Saikosaponin D isolated from Bupleurum falcatum inhibits selectin-mediated cell adhesion. Molecules. 2014;19:20340-9 pubmed publisher
Schneider Hohendorf T, Rossaint J, Mohan H, Böning D, Breuer J, Kuhlmann T, et al. VLA-4 blockade promotes differential routes into human CNS involving PSGL-1 rolling of T cells and MCAM-adhesion of TH17 cells. J Exp Med. 2014;211:1833-46 pubmed publisher
Frommhold D, Kamphues A, Hepper I, Pruenster M, Lukic I, Socher I, et al. RAGE and ICAM-1 cooperate in mediating leukocyte recruitment during acute inflammation in vivo. Blood. 2010;116:841-9 pubmed publisher
Deban L, Russo R, Sironi M, Moalli F, Scanziani M, Zambelli V, et al. Regulation of leukocyte recruitment by the long pentraxin PTX3. Nat Immunol. 2010;11:328-34 pubmed publisher
Wojciechowski J, Narasipura S, Charles N, Mickelsen D, Rana K, Blair M, et al. Capture and enrichment of CD34-positive haematopoietic stem and progenitor cells from blood circulation using P-selectin in an implantable device. Br J Haematol. 2008;140:673-81 pubmed publisher
Bestebroer J, Poppelier M, Ulfman L, Lenting P, Denis C, Van Kessel K, et al. Staphylococcal superantigen-like 5 binds PSGL-1 and inhibits P-selectin-mediated neutrophil rolling. Blood. 2007;109:2936-43 pubmed

product information

brand :

R&D Systems

master code :

ADP3

SKU :

ADP3-050

product name :

Recombinant Human P-Selectin/CD62P Protein, CF

unit size :

50 ug (also 200 ug)

seo description :

The Recombinant Human P-Selectin/CD62P Protein, CF from R&D Systems is derived from CHO. The Recombinant Human P-Selectin/CD62P Protein, CF has been validated for the following applications: Bioactivity.

target :

P-Selectin/CD62P

category :

Proteins and Enzymes

buffer :

Lyophilized from a 0.2 ╡m filtered solution in PBS containing Calcium and Magnesium with Sorbitol.

conjugate :

Unconjugated

dilution :

Bioactivity

purity :

>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie« Blue Staining.

species :

Human

observed molecular weight :

121-124 kDa, reducing conditions

theoretical molecular weight :

80 kDa

gene symbol :

SELP

details of functionality :

Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When 5 x 104 cells/well are added to human sP-Selectin coated plates (10 ╡g/mL with 100 ╡L/well), 50% will adhere after 1 hour incubation at room temperature.

endotoxin note :

<0.10 EU per 1 ╡g of the protein by the LAL method.

accessionNumbers :

P16109

applications :

Bioactivity

source :

CHO-derived Recombinant Human P-Selectin/CD62P Protein, CF

USD :

325 USD

alt names :

CD62P, CD62P antigen, FLJ45155, GMP140, GRMP, PADGEM, PADGEMantigen CD62), PSEL, selectin P (granule membrane protein 140kDa, antigen CD62), SELP

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 3 months, -20 to -70 ░C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage