Recombinant Human TIMP-3 Protein, CF | R&D Systems 973-TM-010 product information

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human TIMP-3 Protein, CF

catalog :

973-TM-010

quantity :

10 ug

price :

567 USD

more info or order :

R&D Systems product webpage

citations: 29

Reference
Varga A, Németh I, Kemeny L, Varga J, Tiszlavicz L, Kumar D, et al. Elevated Serum Gastrin Is Associated with Melanoma Progression: Putative Role in Increased Migration and Invasion of Melanoma Cells. Int J Mol Sci. 2023;24: pubmed publisher
Abu El Asrar A, Nawaz M, Ahmad A, Dillemans L, Siddiquei M, Allegaert E, et al. CD40 Ligand-CD40 Interaction Is an Intermediary between Inflammation and Angiogenesis in Proliferative Diabetic Retinopathy. Int J Mol Sci. 2023;24: pubmed publisher
Tonello R, Silveira Prudente A, Hoon Lee S, Faith Cohen C, Xie W, Paranjpe A, et al. Single-cell analysis of dorsal root ganglia reveals metalloproteinase signaling in satellite glial cells and pain. Brain Behav Immun. 2023;113:401-414 pubmed publisher
Minns A, Qi Y, Yamamoto K, Lee K, Ahnstr xf6 m J, Santamaria S. The C-terminal domains of ADAMTS1 contain exosites involved in its proteoglycanase activity. J Biol Chem. 2023;299:103048 pubmed publisher
Pan B, Zhan X, Li J. MicroRNA-574 Impacts Granulosa Cell Estradiol Production via Targeting TIMP3 and ERK1/2 Signaling Pathway. Front Endocrinol (Lausanne). 2022;13:852127 pubmed publisher
Mwaura A, Riaz M, Maoga J, Mecha E, Omwandho C, Scheiner Bobis G, et al. Role of Betaglycan in TGF-β Signaling and Wound Healing in Human Endometriotic Epithelial Cells and in Endometriosis. Biology (Basel). 2022;11: pubmed publisher
Abu El Asrar A, Ahmad A, Nawaz M, Siddiquei M, De Zutter A, Vanbrabant L, et al. Tissue Inhibitor of Metalloproteinase-3 Ameliorates Diabetes-Induced Retinal Inflammation. Front Physiol. 2021;12:807747 pubmed publisher
Santamaria S, Martin D, Dong X, Yamamoto K, Apte S, Ahnstr xf6 m J. Post-translational regulation and proteolytic activity of the metalloproteinase ADAMTS8. J Biol Chem. 2021;297:101323 pubmed publisher
Cassuto J, Folestad A, Göthlin J, Malchau H, Kärrholm J. Concerted actions by MMPs, ADAMTS and serine proteases during remodeling of the cartilage callus into bone during osseointegration of hip implants. Bone Rep. 2020;13:100715 pubmed publisher
Mitlöhner J, Kaushik R, Niekisch H, Blondiaux A, Gee C, Happel M, et al. Dopamine Receptor Activation Modulates the Integrity of the Perisynaptic Extracellular Matrix at Excitatory Synapses. Cells. 2020;9: pubmed publisher
Su C, Chang Y, Chien M, Hsieh Y, Chen M, Lin C, et al. Loss of TIMP3 by promoter methylation of Sp1 binding site promotes oral cancer metastasis. Cell Death Dis. 2019;10:793 pubmed publisher
Nielsen M, Andersen M, Rittig N, Rødgaard Hansen S, Grønbaek H, Moestrup S, et al. The macrophage-related biomarkers sCD163 and sCD206 are released by different shedding mechanisms. J Leukoc Biol. 2019;106:1129-1138 pubmed publisher
Ruiz Gómez G, Vogel S, Moller S, Pisabarro M, Hempel U. Glycosaminoglycans influence enzyme activity of MMP2 and MMP2/TIMP3 complex formation - Insights at cellular and molecular level. Sci Rep. 2019;9:4905 pubmed publisher
Zhai H, Qi X, Li Z, Zhang W, Li C, Ji L, et al. TIMP‑3 suppresses the proliferation and migration of SMCs from the aortic neck of atherosclerotic AAA in rabbits, via decreased MMP‑2 and MMP‑9 activity, and reduced TNF‑α expression. Mol Med Rep. 2018;18:2061-2067 pubmed publisher
Dave J, Mirabella T, Weatherbee S, Greif D. Pericyte ALK5/TIMP3 Axis Contributes to Endothelial Morphogenesis in the Developing Brain. Dev Cell. 2018;44:665-678.e6 pubmed publisher
Ohrvik H, Logeman B, Turk B, Reinheckel T, Thiele D. Cathepsin Protease Controls Copper and Cisplatin Accumulation via Cleavage of the Ctr1 Metal-binding Ectodomain. J Biol Chem. 2016;291:13905-16 pubmed publisher
Wong K, Zhu F, Khatri I, Huo Q, Spaner D, Gorczynski R. Characterization of CD200 Ectodomain Shedding. PLoS ONE. 2016;11:e0152073 pubmed publisher
Sanz R, Ferraro G, Fournier A. IgLON cell adhesion molecules are shed from the cell surface of cortical neurons to promote neuronal growth. J Biol Chem. 2015;290:4330-42 pubmed publisher
Uchikawa S, Yoda M, Tohmonda T, Kanaji A, Matsumoto M, Toyama Y, et al. ADAM17 regulates IL-1 signaling by selectively releasing IL-1 receptor type 2 from the cell surface. Cytokine. 2015;71:238-45 pubmed publisher
Lajoie L, Congy Jolivet N, Bolzec A, Gouilleux Gruart V, Sicard E, Sung H, et al. ADAM17-mediated shedding of Fc?RIIIA on human NK cells: identification of the cleavage site and relationship with activation. J Immunol. 2014;192:741-51 pubmed publisher
Fröhlich C, Klitgaard M, Noer J, Kotzsch A, Nehammer C, Kronqvist P, et al. ADAM12 is expressed in the tumour vasculature and mediates ectodomain shedding of several membrane-anchored endothelial proteins. Biochem J. 2013;452:97-109 pubmed publisher
Golubkov V, Strongin A. Insights into ectodomain shedding and processing of protein-tyrosine pseudokinase 7 (PTK7). J Biol Chem. 2012;287:42009-18 pubmed publisher
Krstic D, Rodríguez M, Knuesel I. Regulated proteolytic processing of Reelin through interplay of tissue plasminogen activator (tPA), ADAMTS-4, ADAMTS-5, and their modulators. PLoS ONE. 2012;7:e47793 pubmed publisher
Dierker T, Dreier R, Petersen A, Bordych C, Grobe K. Heparan sulfate-modulated, metalloprotease-mediated sonic hedgehog release from producing cells. J Biol Chem. 2009;284:8013-22 pubmed publisher
Sun Q, Weber C, Sohail A, Bernardo M, Toth M, Zhao H, et al. MMP25 (MT6-MMP) is highly expressed in human colon cancer, promotes tumor growth, and exhibits unique biochemical properties. J Biol Chem. 2007;282:21998-2010 pubmed
Fisher K, Pop A, Koh W, Anthis N, Saunders W, Davis G. Tumor cell invasion of collagen matrices requires coordinate lipid agonist-induced G-protein and membrane-type matrix metalloproteinase-1-dependent signaling. Mol Cancer. 2006;5:69 pubmed
Saunders W, Bohnsack B, Faske J, Anthis N, Bayless K, Hirschi K, et al. Coregulation of vascular tube stabilization by endothelial cell TIMP-2 and pericyte TIMP-3. J Cell Biol. 2006;175:179-91 pubmed
Tellier E, Canault M, Rebsomen L, Bonardo B, Juhan Vague I, Nalbone G, et al. The shedding activity of ADAM17 is sequestered in lipid rafts. Exp Cell Res. 2006;312:3969-80 pubmed
Von Tresckow B, Kallen K, Von Strandmann E, Borchmann P, Lange H, Engert A, et al. Depletion of cellular cholesterol and lipid rafts increases shedding of CD30. J Immunol. 2004;172:4324-31 pubmed

product information

brand :

R&D Systems

master code :

973-TM

SKU :

973-TM-010

product name :

Recombinant Human TIMP-3 Protein, CF

unit size :

10 ug

seo description :

The Recombinant Human TIMP-3 Protein, CF from R&D Systems is derived from NS0. The Recombinant Human TIMP-3 Protein, CF has been validated for the following applications: Inhibition Activity.

target :

TIMP-3

category :

Proteins and Enzymes

buffer :

Lyophilized from a 0.2 ╡m filtered solution in Tris and NaCl.

conjugate :

Unconjugated

dilution :

Inhibition Activity

purity :

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie« Blue Staining.

species :

Human

observed molecular weight :

26 kDa, reducing conditions

theoretical molecular weight :

22 kDa

gene symbol :

TIMP3

details of functionality :

Measured by its ability to inhibit human MMP-2 cleavage of a fluorogenic peptide substrate Mca-PLGL-Dpa-AR-NH2 (Catalog # ES001 ). The IC50 value is approximately 3 nM, under conditions the described conditions.

endotoxin note :

<1.0 EU per 1 ╡g of the protein by the LAL method.

accessionNumbers :

P35625

applications :

Inhibition Activity

source :

NS0-derived Recombinant Human TIMP-3 Protein, CF

USD :

567 USD

alt names :

HSMRK222, K222, K222TA2, metalloproteinase inhibitor 3, MIG-5 protein, Protein MIG-5, pseudoinflammatory), SFD, TIMP metallopeptidase inhibitor 3, TIMP3, TIMP-3, tissue inhibitor of metalloproteinase 3 (Sorsby fundus dystrophy, Tissue inhibitor of metalloproteinases 3

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 ░C as supplied. 3 months, -20 to -70 ░C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage