Recombinant Human Cathepsin B Protein, CF | R&D Systems 953-CY product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human Cathepsin B Protein, CF

catalog :

953-CY

quantity :

10 ug

price :

339 USD

citations: 12

Reference
Adem S, Jain S, Sveiven M, Zhou X, O Donoghue A, Hall D. Giant magnetoresistive biosensors for real-time quantitative detection of protease activity. Sci Rep. 2020;10:7941 pubmed publisher
Høglund R, Torsetnes S, Lossius A, Bogen B, Homan E, BREMEL R, et al. Human Cysteine Cathepsins Degrade Immunoglobulin G In Vitro in a Predictable Manner. Int J Mol Sci. 2019;20: pubmed publisher
Huo D, Zhu J, Chen G, Chen Q, Zhang C, Luo X, et al. Eradication of unresectable liver metastasis through induction of tumour specific energy depletion. Nat Commun. 2019;10:3051 pubmed publisher
Lee C, Stankowski J, Chew J, Cook C, Lam Y, Almeida S, et al. The lysosomal protein cathepsin L is a progranulin protease. Mol Neurodegener. 2017;12:55 pubmed publisher
Larimer B, Wehrenberg Klee E, Dubois F, Mehta A, Kalomeris T, Flaherty K, et al. Granzyme B PET Imaging as a Predictive Biomarker of Immunotherapy Response. Cancer Res. 2017;77:2318-2327 pubmed publisher
Shivanna V, Kim Y, Chang K. Endosomal acidification and cathepsin L activity is required for calicivirus replication. Virology. 2014;464-465:287-295 pubmed publisher
Jiang H, Cheng X, Shi G, Hu L, Inoue A, Yamamura Y, et al. Cathepsin K-mediated Notch1 activation contributes to neovascularization in response to hypoxia. Nat Commun. 2014;5:3838 pubmed publisher
Bruchard M, Mignot G, Derangère V, Chalmin F, Chevriaux A, Vegran F, et al. Chemotherapy-triggered cathepsin B release in myeloid-derived suppressor cells activates the Nlrp3 inflammasome and promotes tumor growth. Nat Med. 2013;19:57-64 pubmed publisher
Guery L, Benikhlef N, Gautier T, Paul C, Jego G, Dufour E, et al. Fine-tuning nucleophosmin in macrophage differentiation and activation. Blood. 2011;118:4694-704 pubmed publisher
Burster T, Marin Esteban V, Boehm B, Dunn S, Rotzschke O, Falk K, et al. Design of protease-resistant myelin basic protein-derived peptides by cleavage site directed amino acid substitutions. Biochem Pharmacol. 2007;74:1514-23 pubmed
Cheng T, Hitomi K, van Vlijmen Willems I, de Jongh G, Yamamoto K, Nishi K, et al. Cystatin M/E is a high affinity inhibitor of cathepsin V and cathepsin L by a reactive site that is distinct from the legumain-binding site. A novel clue for the role of cystatin M/E in epidermal cornification. J Biol Chem. 2006;281:15893-9 pubmed
Gocheva V, Zeng W, Ke D, Klimstra D, Reinheckel T, Peters C, et al. Distinct roles for cysteine cathepsin genes in multistage tumorigenesis. Genes Dev. 2006;20:543-56 pubmed

product information

brand :

R&D Systems

master code :

953-CY

SKU :

953-CY-010

product name :

Recombinant Human Cathepsin B Protein, CF

description :

The Recombinant Human Cathepsin B Protein, CF from R&D Systems is derived from NS0. The Recombinant Human Cathepsin B Protein, CF has been validated for the following applications: Enzyme Activity.

target :

Cathepsin B

category :

Proteins and Enzymes

unit size :

10 ug

buffer :

Supplied as a 0.2 µm filtered solution in Tris and NaCl.

conjugate :

Unconjugated

purity :

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

species :

Human

observed molecular weight :

43 kDa and 36 kDa, reducing conditions

theoretical molecular weight :

37 kDa (Pro) 29 kDa (Mature)

gene symbol :

CTSB

details of functionality :

Measured by its ability to cleave the fluorogenic peptide substrate Z-LR-AMC (Catalog # ES008 ). The specific activity is 2,500 pmol/min/µg, as measured under the described conditions.

endotoxin note :

<1.0 EU per 1 µg of the protein by the LAL method.

catalog number base :

953-CY

accessionNumbers :

P07858

applications :

Enzyme Activity

source :

Mouse myeloma cell line, NS0-derived human Cathepsin B protein Arg18-Ile339 (pro) & Phe74-Ile339 (mature), both with a C-terminal 10-His tag

2020 USD :

339

2021 USD :

339 USD

alt names :

APP secretase, APPS, cathepsin B, Cathepsin B1, CPSBamyloid precursor protein secretase, CTSB, cysteine protease, EC 3.4.22, EC 3.4.22.1

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening.