product summary
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company name :
R&D Systems
product type :
protein
product name :
Recombinant Human Cathepsin L Protein, CF
catalog :
952-CY-010
quantity :
10 ug
price :
432 USD
more info or order :
citations: 29
Reference
Chazot A, Zimberger C, Feracci M, Moussa A, Good S, Sommadossi J, et al. The activation cascade of the broad-spectrum antiviral bemnifosbuvir characterized at atomic resolution. PLoS Biol. 2024;22:e3002743 pubmed publisher
Sutton V, Watt S, Akhlaghi H, Cipolla D, Chen K, LaSala D, et al. Pharmacologic inhibition of dipeptidyl peptidase 1 (cathepsin C) does not block in vitro granzyme-mediated target cell killing by CD8 T or NK cells. Front Pharmacol. 2024;15:1396710 pubmed publisher
Heggelund J, Das S, Stamnaes J, Iversen R, Sollid L. Autoantibody binding and unique enzyme-substrate intermediate conformation of human transglutaminase 3. Nat Commun. 2023;14:6216 pubmed publisher
Nirschl C, Brodkin H, Domonkos C, Dwyer C, Hicklin D, Ismail N, et al. mWTX-330, an IL-12 INDUKINE Molecule, Activates and Reshapes Tumor-Infiltrating CD8+ T and NK Cells to Generate Antitumor Immunity. Cancer Immunol Res. 2023;11:962-977 pubmed publisher
Kim S, Lee K, Choi H, Kim E, Kang S, Han M, et al. Hederacolchiside A1 Suppresses Autophagy by Inhibiting Cathepsin C and Reduces the Growth of Colon Cancer. Cancers (Basel). 2023;15: pubmed publisher
Luebben A, Bender D, Becker S, Crowther L, Erven I, Hofmann K, et al. Cln5 represents a new type of cysteine-based S-depalmitoylase linked to neurodegeneration. Sci Adv. 2022;8:eabj8633 pubmed publisher
Shapira T, Monreal I, Dion S, Buchholz D, Imbiakha B, Olmstead A, et al. A TMPRSS2 inhibitor acts as a pan-SARS-CoV-2 prophylactic and therapeutic. Nature. 2022;605:340-348 pubmed publisher
Deng W, Bai Y, Deng F, Pan Y, Mei S, Zheng Z, et al. Streptococcal pyrogenic exotoxin B cleaves GSDMA and triggers pyroptosis. Nature. 2022;602:496-502 pubmed publisher
Linhares D, Faria F, Kodama R, Amorim A, Portaro F, Trevisan Silva D, et al. Novel Cysteine Protease Inhibitor Derived from the Haementeria vizottoi Leech: Recombinant Expression, Purification, and Characterization. Toxins (Basel). 2021;13: pubmed publisher
Li J, Liu S, Shi J, Zhu H. Activation of Tenofovir Alafenamide and Sofosbuvir in the Human Lung and Its Implications in the Development of Nucleoside/Nucleotide Prodrugs for Treating SARS-CoV-2 Pulmonary Infection. Pharmaceutics. 2021;13: pubmed publisher
Kim S, Lee S, Kim N, Joo M, Lee K, Lee M, et al. Decursin inhibits cell growth and autophagic flux in gastric cancer via suppression of cathepsin C. Am J Cancer Res. 2021;11:1304-1320 pubmed
Mittler E, Alkutkar T, Jangra R, Chandran K. Direct Intracellular Visualization of Ebola Virus-Receptor Interaction by In Situ Proximity Ligation. MBio. 2021;12: pubmed publisher
Høglund R, Torsetnes S, Lossius A, Bogen B, Homan E, BREMEL R, et al. Human Cysteine Cathepsins Degrade Immunoglobulin G In Vitro in a Predictable Manner. Int J Mol Sci. 2019;20: pubmed publisher
Dana D, Garcia J, Bhuiyan A, Rathod P, Joo L, Novoa D, et al. Cell penetrable, clickable and tagless activity-based probe of human cathepsin L. Bioorg Chem. 2019;85:505-514 pubmed publisher
Blau R, Epshtein Y, Pisarevsky E, Tiram G, Israeli Dangoor S, Yeini E, et al. Image-guided surgery using near-infrared Turn-ON fluorescent nanoprobes for precise detection of tumor margins. Theranostics. 2018;8:3437-3460 pubmed publisher
Schaal J, Tran D, Subramanian A, Patel R, Laragione T, Roberts K, et al. Suppression and resolution of autoimmune arthritis by rhesus ?-defensin-1, an immunomodulatory macrocyclic peptide. PLoS ONE. 2017;12:e0187868 pubmed publisher
Lee C, Stankowski J, Chew J, Cook C, Lam Y, Almeida S, et al. The lysosomal protein cathepsin L is a progranulin protease. Mol Neurodegener. 2017;12:55 pubmed publisher
Zhao X, Howell K, He S, Brannan J, Wec A, Davidson E, et al. Immunization-Elicited Broadly Protective Antibody Reveals Ebolavirus Fusion Loop as a Site of Vulnerability. Cell. 2017;169:891-904.e15 pubmed publisher
Piper A, Ross S, Redpath G, Lemckert F, Woolger N, Bournazos A, et al. Enzymatic cleavage of myoferlin releases a dual C2-domain module linked to ERK signalling. Cell Signal. 2017;33:30-40 pubmed publisher
Shivanna V, Kim Y, Chang K. Endosomal acidification and cathepsin L activity is required for calicivirus replication. Virology. 2014;464-465:287-295 pubmed publisher
Jiang H, Cheng X, Shi G, Hu L, Inoue A, Yamamura Y, et al. Cathepsin K-mediated Notch1 activation contributes to neovascularization in response to hypoxia. Nat Commun. 2014;5:3838 pubmed publisher
Guery L, Benikhlef N, Gautier T, Paul C, Jego G, Dufour E, et al. Fine-tuning nucleophosmin in macrophage differentiation and activation. Blood. 2011;118:4694-704 pubmed publisher
Zou F, Schäfer N, Palesch D, Brücken R, Beck A, Sienczyk M, et al. Regulation of cathepsin G reduces the activation of proinsulin-reactive T cells from type 1 diabetes patients. PLoS ONE. 2011;6:e22815 pubmed publisher
Gonzalez R, Seurynck Servoss S, Crowley S, Brown M, Omenn G, Hayes D, et al. Development and validation of sandwich ELISA microarrays with minimal assay interference. J Proteome Res. 2008;7:2406-14 pubmed publisher
Hamilton G, Colbert J, Schuettelkopf A, Watts C. Cystatin F is a cathepsin C-directed protease inhibitor regulated by proteolysis. EMBO J. 2008;27:499-508 pubmed publisher
Burster T, Marin Esteban V, Boehm B, Dunn S, Rotzschke O, Falk K, et al. Design of protease-resistant myelin basic protein-derived peptides by cleavage site directed amino acid substitutions. Biochem Pharmacol. 2007;74:1514-23 pubmed
Methot N, Rubin J, Guay D, Beaulieu C, Ethier D, Reddy T, et al. Inhibition of the activation of multiple serine proteases with a cathepsin C inhibitor requires sustained exposure to prevent pro-enzyme processing. J Biol Chem. 2007;282:20836-46 pubmed
Cheng T, Hitomi K, van Vlijmen Willems I, de Jongh G, Yamamoto K, Nishi K, et al. Cystatin M/E is a high affinity inhibitor of cathepsin V and cathepsin L by a reactive site that is distinct from the legumain-binding site. A novel clue for the role of cystatin M/E in epidermal cornification. J Biol Chem. 2006;281:15893-9 pubmed
Gocheva V, Zeng W, Ke D, Klimstra D, Reinheckel T, Peters C, et al. Distinct roles for cysteine cathepsin genes in multistage tumorigenesis. Genes Dev. 2006;20:543-56 pubmed
product information
brand :
R&D Systems
master code :
952-CY
SKU :
952-CY-010
product name :
Recombinant Human Cathepsin L Protein, CF
unit size :
10 ug
seo description :
The Recombinant Human Cathepsin L Protein, CF from R&D Systems is derived from NS0. The Recombinant Human Cathepsin L Protein, CF has been validated for the following applications: Enzyme Activity.
target :
Cathepsin L
category :
Proteins and Enzymes
buffer :
Supplied as a 0.2 ╡m filtered solution in Sodium Acetate and NaCl.
conjugate :
Unconjugated
dilution :
Enzyme Activity
purity :
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
species :
Human
observed molecular weight :
36 kDa, reducing conditions
theoretical molecular weight :
26 kDa
gene symbol :
CTSL
details of functionality :
Measured by its ability to cleave the fluorogenic peptide substrate Z-LR-AMC (Catalog # ES008 ). The specific activity is 25,000 pmol/min/╡g, as measured under the described conditions.
endotoxin note :
<1.0 EU per 1 ╡g of the protein by the LAL method.
accessionNumbers :
P07711
applications :
Enzyme Activity
source :
NS0-derived Recombinant Human Cathepsin L Protein, CF
USD :
432 USD
alt names :
cathepsin L, cathepsin L1, CATL, CTSL, CTSL1, CTSLEC 3.4.22.15, EC 3.4.22, FLJ31037, Major excreted protein, MEP
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 ░C as supplied. 3 months, -20 to -70 ░C under sterile conditions after opening.
more info or order :
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.