Recombinant Human SPARC Protein, CF | R&D Systems 941-SP-050 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human SPARC Protein, CF

catalog :

941-SP-050

quantity :

50 ug

price :

432 USD

citations: 7

Reference
Okada T, Suzuki H, Travis Z, Altay O, Tang J, Zhang J. SPARC Aggravates Blood-Brain Barrier Disruption via Integrin αVβ3/MAPKs/MMP-9 Signaling Pathway after Subarachnoid Hemorrhage. Oxid Med Cell Longev. 2021;2021:9739977 pubmed publisher
Chang C, Yen M, Liao S, Hsu Y, Lai C, Chang K, et al. Secreted Protein Acidic and Rich in Cysteine (SPARC) Enhances Cell Proliferation, Migration, and Epithelial Mesenchymal Transition, and SPARC Expression is Associated with Tumor Grade in Head and Neck Cancer. Int J Mol Sci. 2017;18: pubmed publisher
Alkabie S, Basivireddy J, Zhou L, Roskams J, Rieckmann P, Quandt J. SPARC expression by cerebral microvascular endothelial cells in vitro and its influence on blood-brain barrier properties. J Neuroinflammation. 2016;13:225 pubmed publisher
Aguilera K, Rivera L, Hur H, Carbon J, Toombs J, Goldstein C, et al. Collagen signaling enhances tumor progression after anti-VEGF therapy in a murine model of pancreatic ductal adenocarcinoma. Cancer Res. 2014;74:1032-44 pubmed publisher
Giallongo C, La Cava P, Tibullo D, Barbagallo I, Parrinello N, Cupri A, et al. SPARC expression in CML is associated to imatinib treatment and to inhibition of leukemia cell proliferation. BMC Cancer. 2013;13:60 pubmed publisher
Brellier F, Ruggiero S, Zwolanek D, Martina E, Hess D, Brown Luedi M, et al. SMOC1 is a tenascin-C interacting protein over-expressed in brain tumors. Matrix Biol. 2011;30:225-33 pubmed publisher
Kirouac D, Ito C, Csaszar E, Roch A, Yu M, Sykes E, et al. Dynamic interaction networks in a hierarchically organized tissue. Mol Syst Biol. 2010;6:417 pubmed publisher

product information

master code :

941-SP

SKU :

941-SP-050

product name :

Recombinant Human SPARC Protein, CF

unit size :

50 ug

description :

The Recombinant Human SPARC Protein, CF from R&D Systems is derived from NS0. The Recombinant Human SPARC Protein, CF has been validated for the following applications: Bioactivity.

target :

SPARC

category :

Proteins and Enzymes

buffer :

Lyophilized from a 0.2 ╡m filtered solution in PBS.

conjugate :

Unconjugated

purity :

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie« Blue Staining.

species :

Human

observed molecular weight :

40-50 kDa, reducing conditions

theoretical molecular weight :

34 kDa

gene symbol :

SPARC

details of functionality :

Measured by its ability to inhibit the cell growth of Mv1Lu mink lung epithelial cells. Schiemann,áB.J. etáal . (2003) Mol. Biol. Cell. 14 :3977. The ED50 for this effect is 0.75-3.0 ╡g/mL.

endotoxin note :

<0.10 EU per 1 ╡g of the protein by the LAL method.

accessionNumbers :

NP_003109

applications :

Bioactivity

source long :

Mouse myeloma cell line, NS0-derived human SPARC protein Ala18-Ile303, with a C-terminal 10-His tag

source short :

NS0

USD :

432 USD

alt names :

Basement-membrane protein 40, BM-40, ONcysteine-rich protein, osteonectin, Secreted protein acidic and rich in cysteine, secreted protein, acidic, cysteine-rich (osteonectin)

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 3 months, -20 to -70 ░C under sterile conditions after reconstitution.