Recombinant Human SIRP alpha/CD172a His-tag Protein, CF | R&D Systems 9378-SA-050 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human SIRP alpha/CD172a His-tag Protein, CF

catalog :

9378-SA-050

quantity :

50 ug

price :

443 USD

citations: 3

Reference
Antonsen K, Jensen A, Carstensen M, Nejsum L, Sorensen B, Etzerodt A, et al. Proinflammatory polarization strongly reduces human macrophage in vitro phagocytosis of tumor cells in response to CD47 blockade. Eur J Immunol. 2024;54:e2350824 pubmed publisher
Vladimirova Y, M xf8 lmer M, Antonsen K, M xf8 ller N, Rittig N, Nielsen M, et al. A New Serum Macrophage Checkpoint Biomarker for Innate Immunotherapy: Soluble Signal-Regulatory Protein Alpha (sSIRPα). Biomolecules. 2022;12: pubmed publisher
van Bommel P, He Y, Schepel I, Hendriks M, Wiersma V, van Ginkel R, et al. CD20-selective inhibition of CD47-SIRPα "don't eat me" signaling with a bispecific antibody-derivative enhances the anticancer activity of daratumumab, alemtuzumab and obinutuzumab. Oncoimmunology. 2018;7:e1386361 pubmed publisher

product information

master code :

9378-SA

SKU :

9378-SA-050

product name :

Recombinant Human SIRP alpha/CD172a His-tag Protein, CF

unit size :

50 ug

description :

The Recombinant Human SIRP alpha/CD172a His-tag Protein, CF from R&D Systems is derived from CHO. The Recombinant Human SIRP alpha/CD172a His-tag Protein, CF has been validated for the following applications: Bioactivity.

target :

SIRP alpha/CD172a

category :

Proteins and Enzymes

buffer :

Lyophilized from a 0.2 ╡m filtered solution in PBS.

conjugate :

Unconjugated

purity :

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie« Blue Staining.

species :

Human

observed molecular weight :

44-63 kDa, reducing conditions

theoretical molecular weight :

38 kDa

details of functionality :

Measured by its binding ability in a functional ELISA. When Recombinant Human SIRP alpha /CD172a His-tag is coated at 0.5 ╡g/mL, 100 ╡L/well, Recombinant Human CD47 Fc Chimera (Catalog # 4670-CD ) binds with an ED50 of 0.04-0.24 ╡g/mL.

endotoxin note :

<0.10 EU per 1 ╡g of the protein by the LAL method.

top caption :

Recombinant Human SIRP alpha/CD172a His-tag Protein Bioactivity

accessionNumbers :

NP_542970.1

applications :

Bioactivity

source long :

Chinese Hamster Ovary cell line, CHO-derived human SIRP alpha/CD172a protein Gly27 & Glu31-Arg370, with a C-terminal 6-His tag

source short :

CHO

USD :

443 USD

alt names :

Bit, BITbrain-immunoglobulin-like molecule with tyrosine-based activation motifs, Brain Ig-like molecule with tyrosine-based activation motifs, CD172 antigen-like family member A, CD172a, CD172a antigen, Inhibitory receptor SHPS-1, Macrophage fusion receptor, MFR, MFRtyrosine phosphatase SHP substrate 1, MYD1, MYD-1, MyD-1 antigen, P84, protein tyrosine phosphatase, non-receptor type substrate 1, PTPNS1, SHP substrate 1, SHPS1, SHPS-1, SHPS1CD172A, signal-regulatory protein alpha, Signal-regulatory protein alpha-1, Signal-regulatory protein alpha-2, Signal-regulatory protein alpha-3, SIRPA, SIRPalpha, SIRP-ALPHA-1, Sirp-alpha-2, SIRPalpha2, Sirp-alpha-3, SIRPtyrosine-protein phosphatase non-receptor type substrate 1

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 3 months, -20 to -70 ░C under sterile conditions after reconstitution.