Recombinant Human ACE-2 Protein, CF | R&D Systems 933-ZN product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human ACE-2 Protein, CF

catalog :

933-ZN

quantity :

10 ug (also 100 ug)

price :

339 USD

citations: 11

Reference
Tai W, He L, Zhang X, Pu J, Voronin D, Jiang S, et al. Characterization of the receptor-binding domain (RBD) of 2019 novel coronavirus: implication for development of RBD protein as a viral attachment inhibitor and vaccine. Cell Mol Immunol. 2020;17:613-620 pubmed publisher
Larouche Lebel É, Loughran K, Oyama M, Solter P, Laughlin D, Sanchez M, et al. Plasma and tissue angiotensin-converting enzyme 2 activity and plasma equilibrium concentrations of angiotensin peptides in dogs with heart disease. J Vet Intern Med. 2019;33:1571-1584 pubmed publisher
South A, Nixon P, Chappell M, Diz D, Russell G, Shaltout H, et al. Obesity is Associated with Higher Blood Pressure and Higher Levels of Angiotensin II but Lower Angiotensin-(1-7) in Adolescents Born Preterm. J Pediatr. 2019;205:55-60.e1 pubmed publisher
Wang L, de Kloet A, Smeltzer M, Cahill K, Hiller H, Bruce E, et al. Coupling corticotropin-releasing-hormone and angiotensin converting enzyme 2 dampens stress responsiveness in male mice. Neuropharmacology. 2018;133:85-93 pubmed publisher
Kehoe P, Wong S, Al Mulhim N, Palmer L, Miners J. Angiotensin-converting enzyme 2 is reduced in Alzheimer's disease in association with increasing amyloid-? and tau pathology. Alzheimers Res Ther. 2016;8:50 pubmed
Heurich A, Hofmann Winkler H, Gierer S, Liepold T, Jahn O, Pohlmann S. TMPRSS2 and ADAM17 cleave ACE2 differentially and only proteolysis by TMPRSS2 augments entry driven by the severe acute respiratory syndrome coronavirus spike protein. J Virol. 2014;88:1293-307 pubmed publisher
Zhou Y, Lu K, Pfefferle S, Bertram S, Glowacka I, Drosten C, et al. A single asparagine-linked glycosylation site of the severe acute respiratory syndrome coronavirus spike glycoprotein facilitates inhibition by mannose-binding lectin through multiple mechanisms. J Virol. 2010;84:8753-64 pubmed publisher
Epelman S, Tang W, Chen S, Van Lente F, Francis G, Sen S. Detection of soluble angiotensin-converting enzyme 2 in heart failure: insights into the endogenous counter-regulatory pathway of the renin-angiotensin-aldosterone system. J Am Coll Cardiol. 2008;52:750-4 pubmed publisher
de Lang A, Osterhaus A, Haagmans B. Interferon-gamma and interleukin-4 downregulate expression of the SARS coronavirus receptor ACE2 in Vero E6 cells. Virology. 2006;353:474-81 pubmed
Follis K, York J, Nunberg J. Furin cleavage of the SARS coronavirus spike glycoprotein enhances cell-cell fusion but does not affect virion entry. Virology. 2006;350:358-69 pubmed
Imai Y, Kuba K, Rao S, Huan Y, Guo F, Guan B, et al. Angiotensin-converting enzyme 2 protects from severe acute lung failure. Nature. 2005;436:112-6 pubmed

product information

brand :

R&D Systems

master code :

933-ZN

SKU :

933-ZN-010

product name :

Recombinant Human ACE-2 Protein, CF

description :

The Recombinant Human ACE-2 Protein, CF from R&D Systems is derived from NS0. The Recombinant Human ACE-2 Protein, CF has been validated for the following applications: Enzyme Activity.

target :

ACE-2

category :

Proteins and Enzymes

unit size :

10 ug (also 100 ug)

buffer :

Supplied as a 0.2 µm filtered solution in Tris, NaCl, ZnCl2 and Glycerol.

conjugate :

Unconjugated

purity :

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

species :

Human

observed molecular weight :

101-111 kDa, reducing conditions

theoretical molecular weight :

85 kDa

gene symbol :

ACE2

details of functionality :

Measured by its binding ability in a functional ELISA with Recombinant Viral SARS-CoV-2 S Protein RBD Fc Chimera (Catalog # 10499-CV). Measured by its ability to cleave a fluorogenic peptide substrate, Mca-YVADAPK(Dnp)-OH (Catalog # ES007 ). The specific activity is 800 pmol/min/µg, as measured under the described conditions.

endotoxin note :

<1.0 EU per 1 µg of the protein by the LAL method.

catalog number base :

933-ZN

accessionNumbers :

Q9BYF1

applications :

Enzyme Activity

source :

Mouse myeloma cell line, NS0-derived human ACE-2 protein Gln18-Ser740, with a C-terminal 10-His tag

2020 USD :

339

2021 USD :

339 USD

alt names :

ACE2, ACEH, ACEHangiotensin I converting enzyme 2, ACE-related carboxypeptidase, angiotensin I converting enzyme (peptidyl-dipeptidase A) 2, angiotensin-converting enzyme 2, Angiotensin-converting enzyme homolog, DKFZp434A014, EC 3.4.17, EC 3.4.17.23, Metalloprotease MPROT15

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening.