Recombinant Human ACE-2 Protein, CF | R&D Systems 933-ZN-010 product information

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product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human ACE-2 Protein, CF

catalog :

933-ZN-010

quantity :

10 ug (also 100 ug)

price :

432 USD

citations: 30

Reference
Klegerman M, Peng T, Seferovich I, Rahbar M, Hessabi M, Tahanan A, et al. Absolute concentration estimation of COVID-19 convalescent and post-vaccination IgG antibodies. PLoS ONE. 2024;19:e0311777 pubmed publisher
Gaglione S, Rosales T, Schmidt Hong L, Baker B, Birnbaum M. SARS-CoV-2 spike does not interact with the T cell receptor or directly activate T cells. Proc Natl Acad Sci U S A. 2024;121:e2406615121 pubmed publisher
Bhargavan B, Kanmogne G. SARS-CoV-2 Spike Proteins and Cell-Cell Communication Induce P-Selectin and Markers of Endothelial Injury, NETosis, and Inflammation in Human Lung Microvascular Endothelial Cells and Neutrophils: Implications for the Pathogenesis of COVID-19 Coagulop. Int J Mol Sci. 2023;24: pubmed publisher
Bouillet L, Deroux A, Benmarce M, Gu xe9 rin C, Bouvet L, Garnier O, et al. Molecular Mechanisms of Endothelialitis in SARS-CoV-2 Infection: Evidence for VE-Cadherin Cleavage by ACE2. Int J Mol Sci. 2023;24: pubmed publisher
Suresh V, Sheik D, Detomasi T, Zhao T, Zepeda T, Saladi S, et al. A Prototype Assay Multiplexing SARS-CoV-2 3CL-Protease and Angiotensin-Converting Enzyme 2 for Saliva-Based Diagnostics in COVID-19. Biosensors (Basel). 2023;13: pubmed publisher
Fu L, Gilham D, Stotz S, Sarsons C, Rakai B, Tsujikawa L, et al. Dual mechanism: Epigenetic inhibitor apabetalone reduces SARS-CoV-2 Delta and Omicron variant spike binding and attenuates SARS-CoV-2 RNA induced inflammation. Int Immunopharmacol. 2023;117:109929 pubmed publisher
Guo X, Cao J, Cai J, Wu J, Huang J, Asthana P, et al. Control of SARS-CoV-2 infection by MT1-MMP-mediated shedding of ACE2. Nat Commun. 2022;13:7907 pubmed publisher
Kim J, Min S, Lee J, Shin H, Choi H, Yang H, et al. Development and Characterization of Phage-Display-Derived Novel Human Monoclonal Antibodies against the Receptor Binding Domain of SARS-CoV-2. Biomedicines. 2022;10: pubmed publisher
Reindl Schwaighofer R, H xf6 dlmoser S, Domenig O, Krenn K, Eskandary F, Krenn S, et al. The systemic renin-angiotensin system in COVID-19. Sci Rep. 2022;12:20117 pubmed publisher
Bhargavan B, Kanmogne G. SARS-CoV-2 Spike Proteins and Cell-Cell Communication Inhibits TFPI and Induces Thrombogenic Factors in Human Lung Microvascular Endothelial Cells and Neutrophils: Implications for COVID-19 Coagulopathy Pathogenesis. Int J Mol Sci. 2022;23: pubmed publisher
Haddad M, Gaudreault R, Sasseville G, Nguyen P, Wiebe H, van de Ven T, et al. Molecular Interactions of Tannic Acid with Proteins Associated with SARS-CoV-2 Infectivity. Int J Mol Sci. 2022;23: pubmed publisher
Yamaguchi T, Hoshizaki M, Minato T, Nirasawa S, Asaka M, Niiyama M, et al. ACE2-like carboxypeptidase B38-CAP protects from SARS-CoV-2-induced lung injury. Nat Commun. 2021;12:6791 pubmed publisher
Shukla S, Cho K, Rustagi V, Gao X, Fu X, Zhang S, et al. "Molecular Masks" for ACE2 to Effectively and Safely Block SARS-CoV-2 Virus Entry. Int J Mol Sci. 2021;22: pubmed publisher
Polak Y, Speth R. Metabolism of angiotensin peptides by angiotensin converting enzyme 2 (ACE2) and analysis of the effect of excess zinc on ACE2 enzymatic activity. Peptides. 2021;137:170477 pubmed publisher
Larouche Lebel É, Loughran K, Huh T, Oyama M. Effect of angiotensin receptor blockers and angiotensin converting enzyme 2 on plasma equilibrium angiotensin peptide concentrations in dogs with heart disease. J Vet Intern Med. 2021;35:22-32 pubmed publisher
Huh T, Larouche Lebel É, Loughran K, Oyama M. Effect of angiotensin receptor blockers and angiotensin-converting enzyme 2 on plasma equilibrium angiotensin peptide concentrations in cats with heart disease. J Vet Intern Med. 2021;35:33-42 pubmed publisher
Lu J, Sun P. High affinity binding of SARS-CoV-2 spike protein enhances ACE2 carboxypeptidase activity. J Biol Chem. 2020;295:18579-18588 pubmed publisher
Lu J, Sun P. High affinity binding of SARS-CoV-2 spike protein enhances ACE2 carboxypeptidase activity. bioRxiv. 2020;: pubmed publisher
Song J, Qu H, Hu B, Bi C, Li M, Wang L, et al. Physiological cyclic stretch up-regulates angiotensin-converting enzyme 2 expression to reduce proliferation and migration of vascular smooth muscle cells. Biosci Rep. 2020;40: pubmed publisher
Tai W, He L, Zhang X, Pu J, Voronin D, Jiang S, et al. Characterization of the receptor-binding domain (RBD) of 2019 novel coronavirus: implication for development of RBD protein as a viral attachment inhibitor and vaccine. Cell Mol Immunol. 2020;17:613-620 pubmed publisher
Larouche Lebel É, Loughran K, Oyama M, Solter P, Laughlin D, Sanchez M, et al. Plasma and tissue angiotensin-converting enzyme 2 activity and plasma equilibrium concentrations of angiotensin peptides in dogs with heart disease. J Vet Intern Med. 2019;33:1571-1584 pubmed publisher
South A, Nixon P, Chappell M, Diz D, Russell G, Shaltout H, et al. Obesity is Associated with Higher Blood Pressure and Higher Levels of Angiotensin II but Lower Angiotensin-(1-7) in Adolescents Born Preterm. J Pediatr. 2019;205:55-60.e1 pubmed publisher
Wang L, de Kloet A, Smeltzer M, Cahill K, Hiller H, Bruce E, et al. Coupling corticotropin-releasing-hormone and angiotensin converting enzyme 2 dampens stress responsiveness in male mice. Neuropharmacology. 2018;133:85-93 pubmed publisher
Kehoe P, Wong S, Al Mulhim N, Palmer L, Miners J. Angiotensin-converting enzyme 2 is reduced in Alzheimer's disease in association with increasing amyloid-? and tau pathology. Alzheimers Res Ther. 2016;8:50 pubmed
Heurich A, Hofmann Winkler H, Gierer S, Liepold T, Jahn O, Pohlmann S. TMPRSS2 and ADAM17 cleave ACE2 differentially and only proteolysis by TMPRSS2 augments entry driven by the severe acute respiratory syndrome coronavirus spike protein. J Virol. 2014;88:1293-307 pubmed publisher
Zhou Y, Lu K, Pfefferle S, Bertram S, Glowacka I, Drosten C, et al. A single asparagine-linked glycosylation site of the severe acute respiratory syndrome coronavirus spike glycoprotein facilitates inhibition by mannose-binding lectin through multiple mechanisms. J Virol. 2010;84:8753-64 pubmed publisher
Epelman S, Tang W, Chen S, Van Lente F, Francis G, Sen S. Detection of soluble angiotensin-converting enzyme 2 in heart failure: insights into the endogenous counter-regulatory pathway of the renin-angiotensin-aldosterone system. J Am Coll Cardiol. 2008;52:750-4 pubmed publisher
de Lang A, Osterhaus A, Haagmans B. Interferon-gamma and interleukin-4 downregulate expression of the SARS coronavirus receptor ACE2 in Vero E6 cells. Virology. 2006;353:474-81 pubmed
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product information

master code :

933-ZN

SKU :

933-ZN-010

product name :

Recombinant Human ACE-2 Protein, CF

unit size :

10 ug (also 100 ug)

description :

The Recombinant Human ACE-2 Protein, CF from R&D Systems is derived from NS0. The Recombinant Human ACE-2 Protein, CF has been validated for the following applications: Enzyme Activity.

target :

ACE-2

category :

Proteins and Enzymes

buffer :

Supplied as a 0.2 ╡m filtered solution in Tris, NaCl, ZnCl2 and Glycerol.

conjugate :

Unconjugated

purity :

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

species :

Human

observed molecular weight :

101-111 kDa, reducing conditions

theoretical molecular weight :

85 kDa

gene symbol :

ACE2

details of functionality :

Measured by its binding ability in a functional ELISA. When Recombinant SARS-CoV-2 Spike RBD Fc Chimera (Catalog # 10499-CV ) is immobilized at 0.2 ╡g/mL (100 ╡L/well), Recombinant Human ACE-2 (Catalog # 933-ZN) binds with an ED50 of 1.00-12.0 ng/mL. Measured by its ability to cleave a fluorogenic peptide substrate, Mca-YVADAPK(Dnp)-OH (Catalog # ES007 ). The specific activity is 800 pmol/min/╡g, as measured under the described conditions.

endotoxin note :

<1.0 EU per 1 ╡g of the protein by the LAL method.

accessionNumbers :

Q9BYF1

applications :

Enzyme Activity

source long :

Mouse myeloma cell line, NS0-derived human ACE-2 protein Gln18-Ser740, with a C-terminal 10-His tag

source short :

NS0

extended description :

Analyzed by SEC-MALS

USD :

432 USD

product details :

Analyzed by SEC-MALS

alt names :

ACE2, ACEH, ACEHangiotensin I converting enzyme 2, ACE-related carboxypeptidase, angiotensin I converting enzyme (peptidyl-dipeptidase A) 2, angiotensin-converting enzyme 2, Angiotensin-converting enzyme homolog, DKFZp434A014, EC 3.4.17, EC 3.4.17.23, Metalloprotease MPROT15

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 ░C as supplied. 3 months, -20 to -70 ░C under sterile conditions after opening.